Conserved amino acids W423 and N424 in receptor-binding domain of SARS-CoV are potential targets for therapeutic monoclonal antibody.
Identifieur interne : 001D84 ( Ncbi/Merge ); précédent : 001D83; suivant : 001D85Conserved amino acids W423 and N424 in receptor-binding domain of SARS-CoV are potential targets for therapeutic monoclonal antibody.
Auteurs : Chao Bian [République populaire de Chine] ; Xiuqin Zhang ; Xingfeng Cai ; Linqi Zhang ; Zhiwei Chen ; Ye Zha ; Ying Xu ; Ke Xu ; Wei Lu ; Linchen Yan ; Jianwei Yuan ; Jiannan Feng ; Pei Hao ; Qidi Wang ; Guoping Zhao ; Gang Liu ; Xueliang Zhu ; Hao Shen ; Bojian Zheng ; Beifen Shen ; Bing SunSource :
- Virology [ 1096-0341 ] ; 2009.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (immunologie), Anticorps antiviraux (isolement et purification), Anticorps antiviraux (usage thérapeutique), Anticorps monoclonaux (immunologie), Anticorps monoclonaux (isolement et purification), Anticorps monoclonaux (usage thérapeutique), Attachement viral, Données de séquences moléculaires, Femelle, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (), Glycoprotéines membranaires (antagonistes et inhibiteurs), Glycoprotéines membranaires (immunologie), Humains, Modèles moléculaires, Protéines de l'enveloppe virale (), Protéines de l'enveloppe virale (antagonistes et inhibiteurs), Protéines de l'enveloppe virale (immunologie), Souris, Souris de lignée BALB C, Structure tertiaire des protéines, Séquence conservée (immunologie), Séquence d'acides aminés, Tests de neutralisation, Virus du SRAS (immunologie), Virus du SRAS (physiologie), Épitopes (immunologie).
- MESH :
- antagonistes et inhibiteurs : Glycoprotéines membranaires, Protéines de l'enveloppe virale.
- immunologie : Anticorps antiviraux, Anticorps monoclonaux, Glycoprotéines membranaires, Protéines de l'enveloppe virale, Séquence conservée, Virus du SRAS, Épitopes.
- isolement et purification : Anticorps antiviraux, Anticorps monoclonaux.
- physiologie : Virus du SRAS.
- usage thérapeutique : Anticorps antiviraux, Anticorps monoclonaux.
- Animaux, Attachement viral, Données de séquences moléculaires, Femelle, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires, Humains, Modèles moléculaires, Protéines de l'enveloppe virale, Souris, Souris de lignée BALB C, Structure tertiaire des protéines, Séquence d'acides aminés, Tests de neutralisation.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Antibodies, Monoclonal (immunology), Antibodies, Monoclonal (isolation & purification), Antibodies, Monoclonal (therapeutic use), Antibodies, Viral (immunology), Antibodies, Viral (isolation & purification), Antibodies, Viral (therapeutic use), Conserved Sequence (immunology), Epitopes (immunology), Female, Humans, Membrane Glycoproteins (antagonists & inhibitors), Membrane Glycoproteins (chemistry), Membrane Glycoproteins (immunology), Mice, Mice, Inbred BALB C, Models, Molecular, Molecular Sequence Data, Neutralization Tests, Protein Structure, Tertiary, SARS Virus (immunology), SARS Virus (physiology), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (antagonists & inhibitors), Viral Envelope Proteins (chemistry), Viral Envelope Proteins (immunology), Virus Attachment.
- MESH :
- chemical , antagonists & inhibitors : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , chemistry : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , immunology : Antibodies, Monoclonal, Antibodies, Viral, Epitopes, Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , isolation & purification : Antibodies, Monoclonal, Antibodies, Viral.
- chemical , therapeutic use : Antibodies, Monoclonal, Antibodies, Viral.
- immunology : Conserved Sequence, SARS Virus.
- physiology : SARS Virus.
- Amino Acid Sequence, Animals, Female, Humans, Mice, Mice, Inbred BALB C, Models, Molecular, Molecular Sequence Data, Neutralization Tests, Protein Structure, Tertiary, Spike Glycoprotein, Coronavirus, Virus Attachment.
Abstract
The receptor-binding domain (RBD) on spike protein of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is the main region interacting with the viral receptor-ACE2 and is a useful target for induction of neutralizing antibodies against SARS-CoV infection. Here we generated two monoclonal antibodies (mAbs), targeting RBD, with marked virus neutralizing activity. The mAbs recognize a new conformational epitope which consists of several discontinuous peptides (aa. 343-367, 373-390 and 411-428) and is spatially located neighboring the receptor-binding motif (RPM) region of the RBD. Importantly, W423 and N424 residues are essential for mAb recognition and are highly conserved among 107 different strains of SARS, indicating that the residues are the most critical in the epitope which is a novel potential target for therapeutic mAbs. A human-mouse chimeric antibody, based upon the original murine mAb, was also constructed and shown to possess good neutralizing activity and high affinity.
DOI: 10.1016/j.virol.2008.09.029
PubMed: 18986662
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 001A34
- to stream PubMed, to step Curation: 001A34
- to stream PubMed, to step Checkpoint: 001920
Links to Exploration step
pubmed:18986662Le document en format XML
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<author><name sortKey="Zhu, Xueliang" sort="Zhu, Xueliang" uniqKey="Zhu X" first="Xueliang" last="Zhu">Xueliang Zhu</name>
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<term>Antibodies, Monoclonal (therapeutic use)</term>
<term>Antibodies, Viral (immunology)</term>
<term>Antibodies, Viral (isolation & purification)</term>
<term>Antibodies, Viral (therapeutic use)</term>
<term>Conserved Sequence (immunology)</term>
<term>Epitopes (immunology)</term>
<term>Female</term>
<term>Humans</term>
<term>Membrane Glycoproteins (antagonists & inhibitors)</term>
<term>Membrane Glycoproteins (chemistry)</term>
<term>Membrane Glycoproteins (immunology)</term>
<term>Mice</term>
<term>Mice, Inbred BALB C</term>
<term>Models, Molecular</term>
<term>Molecular Sequence Data</term>
<term>Neutralization Tests</term>
<term>Protein Structure, Tertiary</term>
<term>SARS Virus (immunology)</term>
<term>SARS Virus (physiology)</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Viral Envelope Proteins (antagonists & inhibitors)</term>
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<term>Données de séquences moléculaires</term>
<term>Femelle</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires ()</term>
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<term>Souris de lignée BALB C</term>
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<term>Séquence d'acides aminés</term>
<term>Tests de neutralisation</term>
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<term>Virus du SRAS (physiologie)</term>
<term>Épitopes (immunologie)</term>
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<term>Séquence conservée</term>
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<term>Animals</term>
<term>Female</term>
<term>Humans</term>
<term>Mice</term>
<term>Mice, Inbred BALB C</term>
<term>Models, Molecular</term>
<term>Molecular Sequence Data</term>
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<term>Protéines de l'enveloppe virale</term>
<term>Souris</term>
<term>Souris de lignée BALB C</term>
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<front><div type="abstract" xml:lang="en">The receptor-binding domain (RBD) on spike protein of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is the main region interacting with the viral receptor-ACE2 and is a useful target for induction of neutralizing antibodies against SARS-CoV infection. Here we generated two monoclonal antibodies (mAbs), targeting RBD, with marked virus neutralizing activity. The mAbs recognize a new conformational epitope which consists of several discontinuous peptides (aa. 343-367, 373-390 and 411-428) and is spatially located neighboring the receptor-binding motif (RPM) region of the RBD. Importantly, W423 and N424 residues are essential for mAb recognition and are highly conserved among 107 different strains of SARS, indicating that the residues are the most critical in the epitope which is a novel potential target for therapeutic mAbs. A human-mouse chimeric antibody, based upon the original murine mAb, was also constructed and shown to possess good neutralizing activity and high affinity.</div>
</front>
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<DateCompleted><Year>2009</Year>
<Month>01</Month>
<Day>16</Day>
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<Month>04</Month>
<Day>07</Day>
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<Title>Virology</Title>
<ISOAbbreviation>Virology</ISOAbbreviation>
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<ArticleTitle>Conserved amino acids W423 and N424 in receptor-binding domain of SARS-CoV are potential targets for therapeutic monoclonal antibody.</ArticleTitle>
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<Abstract><AbstractText>The receptor-binding domain (RBD) on spike protein of severe acute respiratory syndrome-associated coronavirus (SARS-CoV) is the main region interacting with the viral receptor-ACE2 and is a useful target for induction of neutralizing antibodies against SARS-CoV infection. Here we generated two monoclonal antibodies (mAbs), targeting RBD, with marked virus neutralizing activity. The mAbs recognize a new conformational epitope which consists of several discontinuous peptides (aa. 343-367, 373-390 and 411-428) and is spatially located neighboring the receptor-binding motif (RPM) region of the RBD. Importantly, W423 and N424 residues are essential for mAb recognition and are highly conserved among 107 different strains of SARS, indicating that the residues are the most critical in the epitope which is a novel potential target for therapeutic mAbs. A human-mouse chimeric antibody, based upon the original murine mAb, was also constructed and shown to possess good neutralizing activity and high affinity.</AbstractText>
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<ForeName>Chao</ForeName>
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<Author ValidYN="Y"><LastName>Zhang</LastName>
<ForeName>Xiuqin</ForeName>
<Initials>X</Initials>
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<Author ValidYN="Y"><LastName>Cai</LastName>
<ForeName>Xingfeng</ForeName>
<Initials>X</Initials>
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<Author ValidYN="Y"><LastName>Zhang</LastName>
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<Author ValidYN="Y"><LastName>Chen</LastName>
<ForeName>Zhiwei</ForeName>
<Initials>Z</Initials>
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<Author ValidYN="Y"><LastName>Zha</LastName>
<ForeName>Ye</ForeName>
<Initials>Y</Initials>
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<Author ValidYN="Y"><LastName>Xu</LastName>
<ForeName>Ying</ForeName>
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<ForeName>Wei</ForeName>
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<ForeName>Gang</ForeName>
<Initials>G</Initials>
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<Author ValidYN="Y"><LastName>Zhu</LastName>
<ForeName>Xueliang</ForeName>
<Initials>X</Initials>
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<Author ValidYN="Y"><LastName>Shen</LastName>
<ForeName>Hao</ForeName>
<Initials>H</Initials>
</Author>
<Author ValidYN="Y"><LastName>Zheng</LastName>
<ForeName>Bojian</ForeName>
<Initials>B</Initials>
</Author>
<Author ValidYN="Y"><LastName>Shen</LastName>
<ForeName>Beifen</ForeName>
<Initials>B</Initials>
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<Author ValidYN="Y"><LastName>Sun</LastName>
<ForeName>Bing</ForeName>
<Initials>B</Initials>
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<Day>04</Day>
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</ArticleIdList>
<ReferenceList><Reference><Citation>Nat Rev Microbiol. 2003 Dec;1(3):209-18</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15035025</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Cell Res. 2004 Oct;14(5):400-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15450134</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1948-51</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12748314</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2005 Mar;79(5):2678-88</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15708987</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>FEBS Lett. 2005 Apr 11;579(10):2130-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15811330</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2004 Jul;78(13):7217-26</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15194798</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Biochem Biophys Res Commun. 2004 Nov 12;324(2):773-81</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15474494</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Biol Chem. 2006 Nov 10;281(45):34610-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16954221</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Science. 2005 Sep 16;309(5742):1864-8</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16166518</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Biochem Biophys Res Commun. 2004 Dec 10;325(2):445-52</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15530413</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2004 Feb 24;101(8):2536-41</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14983044</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2005 Feb;79(3):1635-44</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15650189</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2005 Sep;79(18):11638-46</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16140741</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virol J. 2005 Aug 25;2:73</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16122388</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Biol Chem. 2004 Jan 30;279(5):3197-201</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14670965</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Nature. 2003 Nov 27;426(6965):450-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14647384</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Biol Chem. 2006 Jun 9;281(23):15829-36</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16597622</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Science. 2003 May 30;300(5624):1394-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12730500</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Virol. 2005 May;79(10):5900-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15857975</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>J Immunol. 2005 Apr 15;174(8):4908-15</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15814718</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Virology. 2005 Mar 30;334(1):134-43</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15749129</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
<ReferenceList><Reference><Citation>Proc Natl Acad Sci U S A. 2005 Feb 15;102(7):2430-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15695582</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>République populaire de Chine</li>
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<name sortKey="Chen, Zhiwei" sort="Chen, Zhiwei" uniqKey="Chen Z" first="Zhiwei" last="Chen">Zhiwei Chen</name>
<name sortKey="Feng, Jiannan" sort="Feng, Jiannan" uniqKey="Feng J" first="Jiannan" last="Feng">Jiannan Feng</name>
<name sortKey="Hao, Pei" sort="Hao, Pei" uniqKey="Hao P" first="Pei" last="Hao">Pei Hao</name>
<name sortKey="Liu, Gang" sort="Liu, Gang" uniqKey="Liu G" first="Gang" last="Liu">Gang Liu</name>
<name sortKey="Lu, Wei" sort="Lu, Wei" uniqKey="Lu W" first="Wei" last="Lu">Wei Lu</name>
<name sortKey="Shen, Beifen" sort="Shen, Beifen" uniqKey="Shen B" first="Beifen" last="Shen">Beifen Shen</name>
<name sortKey="Shen, Hao" sort="Shen, Hao" uniqKey="Shen H" first="Hao" last="Shen">Hao Shen</name>
<name sortKey="Sun, Bing" sort="Sun, Bing" uniqKey="Sun B" first="Bing" last="Sun">Bing Sun</name>
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<name sortKey="Xu, Ke" sort="Xu, Ke" uniqKey="Xu K" first="Ke" last="Xu">Ke Xu</name>
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<name sortKey="Yan, Linchen" sort="Yan, Linchen" uniqKey="Yan L" first="Linchen" last="Yan">Linchen Yan</name>
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<name sortKey="Zhang, Linqi" sort="Zhang, Linqi" uniqKey="Zhang L" first="Linqi" last="Zhang">Linqi Zhang</name>
<name sortKey="Zhang, Xiuqin" sort="Zhang, Xiuqin" uniqKey="Zhang X" first="Xiuqin" last="Zhang">Xiuqin Zhang</name>
<name sortKey="Zhao, Guoping" sort="Zhao, Guoping" uniqKey="Zhao G" first="Guoping" last="Zhao">Guoping Zhao</name>
<name sortKey="Zheng, Bojian" sort="Zheng, Bojian" uniqKey="Zheng B" first="Bojian" last="Zheng">Bojian Zheng</name>
<name sortKey="Zhu, Xueliang" sort="Zhu, Xueliang" uniqKey="Zhu X" first="Xueliang" last="Zhu">Xueliang Zhu</name>
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