SrasV1, Ncbi, Merge, bibRecord, 001D65

Spike protein, S, of human coronavirus HKU1: role in viral life cycle and application in antibody detection.

Identifieur interne : 001D65 ( Ncbi/Merge ); précédent : 001D64; suivant : 001D66

Spike protein, S, of human coronavirus HKU1: role in viral life cycle and application in antibody detection.

Auteurs : Che-Man Chan [Hong Kong] ; Patrick C Y. Woo ; Susanna K P. Lau ; Herman Tse ; Hong-Lin Chen ; Feng Li ; Bo-Jian Zheng ; Ling Chen ; Jian-Dong Huang ; Kwok-Yung Yuen

Source :

Experimental biology and medicine (Maywood, N.J.) [ 1535-3702 ] ; 2008.

RBID : pubmed:18849544

Descripteurs français

English descriptors

KwdEn :
- Animals, Antibodies, Viral (genetics), Antibodies, Viral (immunology), Antibodies, Viral (metabolism), Cell Line, Coronavirus (classification), Coronavirus (genetics), Coronavirus (immunology), Coronavirus (metabolism), Cricetinae, Fluorescein-5-isothiocyanate (metabolism), Fluorescent Antibody Technique, Indirect, Fluorescent Dyes (metabolism), Glycosylation, Humans, Immunization, Kidney (cytology), Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Membrane Glycoproteins (metabolism), Mice, Mice, Inbred BALB C, Physiological Phenomena (genetics), Recombinant Proteins (immunology), Recombinant Proteins (metabolism), Spike Glycoprotein, Coronavirus, Transfection, Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology), Viral Envelope Proteins (metabolism).
MESH :
- chemical , genetics : Antibodies, Viral, Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , immunology : Antibodies, Viral, Membrane Glycoproteins, Recombinant Proteins, Viral Envelope Proteins.
- chemical , metabolism : Antibodies, Viral, Fluorescein-5-isothiocyanate, Fluorescent Dyes, Membrane Glycoproteins, Recombinant Proteins, Viral Envelope Proteins.
- classification : Coronavirus.
- cytology : Kidney.
- genetics : Coronavirus, Physiological Phenomena.
- immunology : Coronavirus.
- metabolism : Coronavirus.
- Animals, Cell Line, Cricetinae, Fluorescent Antibody Technique, Indirect, Glycosylation, Humans, Immunization, Mice, Mice, Inbred BALB C, Spike Glycoprotein, Coronavirus, Transfection.

Abstract

We recently described the discovery, genome, clinical features, genotypes and evolution of a novel and global human respiratory virus named human coronavirus HKU1 (HCoV-HKU1) which is not yet culturable. We expressed a C-terminal FLAG-tagged CoV-HKU1 spike (S) protein by the Semliki Forest Virus (SFV) system and investigated its maturation profile. Pulse chase labeling revealed that S-FLAG was expressed as high-mannose N-glycans of monomers and trimers. It was predominantly cleaved into subdomains S1 and S2 during maturation. S1 was secreted into the medium. Immunofluorescence analysis visualized S along the secretory pathway from endoplasmic reticulum to plasma membrane. Cleavage of S and release of HCoV-HKU1 S pseudotyped virus were inhibited by furin or furin-like enzyme inhibitors. The cell-based expressed full-length S-FLAG could be recognized by the convalescent serum obtained from a patient with HCoV-HKU1 pneumonia. The data suggest that the native form of HCoV-HKU1 spike expressed in our system can be used in developing serological diagnostic assay and in understanding the role of S in the viral life cycle.

DOI: 10.3181/0806-RM-197
PubMed: 18849544

Links toward previous steps (curation, corpus...)

to stream PubMed, to step Corpus: 001A53
to stream PubMed, to step Curation: 001A53
to stream PubMed, to step Checkpoint: 001A14

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pubmed:18849544

Le document en format XML

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<front><div type="abstract" xml:lang="en">We recently described the discovery, genome, clinical features, genotypes and evolution of a novel and global human respiratory virus named human coronavirus HKU1 (HCoV-HKU1) which is not yet culturable. We expressed a C-terminal FLAG-tagged CoV-HKU1 spike (S) protein by the Semliki Forest Virus (SFV) system and investigated its maturation profile. Pulse chase labeling revealed that S-FLAG was expressed as high-mannose N-glycans of monomers and trimers. It was predominantly cleaved into subdomains S1 and S2 during maturation. S1 was secreted into the medium. Immunofluorescence analysis visualized S along the secretory pathway from endoplasmic reticulum to plasma membrane. Cleavage of S and release of HCoV-HKU1 S pseudotyped virus were inhibited by furin or furin-like enzyme inhibitors. The cell-based expressed full-length S-FLAG could be recognized by the convalescent serum obtained from a patient with HCoV-HKU1 pneumonia. The data suggest that the native form of HCoV-HKU1 spike expressed in our system can be used in developing serological diagnostic assay and in understanding the role of S in the viral life cycle.</div>
</front>
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<Abstract><AbstractText>We recently described the discovery, genome, clinical features, genotypes and evolution of a novel and global human respiratory virus named human coronavirus HKU1 (HCoV-HKU1) which is not yet culturable. We expressed a C-terminal FLAG-tagged CoV-HKU1 spike (S) protein by the Semliki Forest Virus (SFV) system and investigated its maturation profile. Pulse chase labeling revealed that S-FLAG was expressed as high-mannose N-glycans of monomers and trimers. It was predominantly cleaved into subdomains S1 and S2 during maturation. S1 was secreted into the medium. Immunofluorescence analysis visualized S along the secretory pathway from endoplasmic reticulum to plasma membrane. Cleavage of S and release of HCoV-HKU1 S pseudotyped virus were inhibited by furin or furin-like enzyme inhibitors. The cell-based expressed full-length S-FLAG could be recognized by the convalescent serum obtained from a patient with HCoV-HKU1 pneumonia. The data suggest that the native form of HCoV-HKU1 spike expressed in our system can be used in developing serological diagnostic assay and in understanding the role of S in the viral life cycle.</AbstractText>
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<MeshHeading><DescriptorName UI="D014759" MajorTopicYN="N">Viral Envelope Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2008</Year>
<Month>10</Month>
<Day>14</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2009</Year>
<Month>1</Month>
<Day>23</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2008</Year>
<Month>10</Month>
<Day>14</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">18849544</ArticleId>
<ArticleId IdType="pii">0806-RM-197</ArticleId>
<ArticleId IdType="doi">10.3181/0806-RM-197</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>Hong Kong</li>
</country>
</list>
<tree><noCountry><name sortKey="Chen, Hong Lin" sort="Chen, Hong Lin" uniqKey="Chen H" first="Hong-Lin" last="Chen">Hong-Lin Chen</name>
<name sortKey="Chen, Ling" sort="Chen, Ling" uniqKey="Chen L" first="Ling" last="Chen">Ling Chen</name>
<name sortKey="Huang, Jian Dong" sort="Huang, Jian Dong" uniqKey="Huang J" first="Jian-Dong" last="Huang">Jian-Dong Huang</name>
<name sortKey="Lau, Susanna K P" sort="Lau, Susanna K P" uniqKey="Lau S" first="Susanna K P" last="Lau">Susanna K P. Lau</name>
<name sortKey="Li, Feng" sort="Li, Feng" uniqKey="Li F" first="Feng" last="Li">Feng Li</name>
<name sortKey="Tse, Herman" sort="Tse, Herman" uniqKey="Tse H" first="Herman" last="Tse">Herman Tse</name>
<name sortKey="Woo, Patrick C Y" sort="Woo, Patrick C Y" uniqKey="Woo P" first="Patrick C Y" last="Woo">Patrick C Y. Woo</name>
<name sortKey="Yuen, Kwok Yung" sort="Yuen, Kwok Yung" uniqKey="Yuen K" first="Kwok-Yung" last="Yuen">Kwok-Yung Yuen</name>
<name sortKey="Zheng, Bo Jian" sort="Zheng, Bo Jian" uniqKey="Zheng B" first="Bo-Jian" last="Zheng">Bo-Jian Zheng</name>
</noCountry>
<country name="Hong Kong"><noRegion><name sortKey="Chan, Che Man" sort="Chan, Che Man" uniqKey="Chan C" first="Che-Man" last="Chan">Che-Man Chan</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>

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   |texte=   Spike protein, S, of human coronavirus HKU1: role in viral life cycle and application in antibody detection.
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Spike protein, S, of human coronavirus HKU1: role in viral life cycle and application in antibody detection.

Spike protein, S, of human coronavirus HKU1: role in viral life cycle and application in antibody detection.

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