A novel strategy for analyzing RNA-protein interactions by surface plasmon resonance biosensor.
Identifieur interne : 001C53 ( Ncbi/Merge ); précédent : 001C52; suivant : 001C54A novel strategy for analyzing RNA-protein interactions by surface plasmon resonance biosensor.
Auteurs : Yaling Yang [République populaire de Chine] ; Quan Wang ; Deyin GuoSource :
- Molecular biotechnology [ 1073-6085 ] ; 2008.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , metabolism : RNA, Viral, Viral Proteins.
- chemical : DNA Primers.
- genetics : SARS Virus.
- instrumentation : Surface Plasmon Resonance.
- Base Sequence, Biosensing Techniques, Electrophoretic Mobility Shift Assay, Nucleic Acid Hybridization.
Abstract
Surface plasmon resonance (SPR) biosensor is a promising technology for its various advantages including the real-time measurement of biomolecular interactions without labeling. A method of hybridizing RNAs on the surface of the streptavidin-coated (SA) sensor chip to study RNA-protein interactions was described in this paper. In our study, it has been shown that the nucleocapsid (N) protein of severe acute respiratory syndrome coronavirus (SARS-CoV) has a high binding affinity for the leader sequence of SARS-CoV genome. Effect of temperature on the RNA-DNA hybridization was also examined. This method can provide the affinity of interactions with high sensitivity. Therefore, it will be useful in screening binding candidates for a given RNA target motif with one chip.
DOI: 10.1007/s12033-008-9066-3
PubMed: 18465270
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A method of hybridizing RNAs on the surface of the streptavidin-coated (SA) sensor chip to study RNA-protein interactions was described in this paper. In our study, it has been shown that the nucleocapsid (N) protein of severe acute respiratory syndrome coronavirus (SARS-CoV) has a high binding affinity for the leader sequence of SARS-CoV genome. Effect of temperature on the RNA-DNA hybridization was also examined. This method can provide the affinity of interactions with high sensitivity. Therefore, it will be useful in screening binding candidates for a given RNA target motif with one chip.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">18465270</PMID><DateCompleted><Year>2008</Year><Month>11</Month><Day>14</Day></DateCompleted><DateRevised><Year>2020</Year><Month>03</Month><Day>25</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">1073-6085</ISSN><JournalIssue CitedMedium="Print"><Volume>40</Volume><Issue>1</Issue><PubDate><Year>2008</Year><Month>Sep</Month></PubDate></JournalIssue><Title>Molecular biotechnology</Title><ISOAbbreviation>Mol. 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