Rapid identification of emerging infectious agents using PCR and electrospray ionization mass spectrometry.
Identifieur interne : 001948 ( Ncbi/Merge ); précédent : 001947; suivant : 001949Rapid identification of emerging infectious agents using PCR and electrospray ionization mass spectrometry.
Auteurs : Rangarajan Sampath [États-Unis] ; Thomas A. Hall ; Christian Massire ; Feng Li ; Lawrence B. Blyn ; Mark W. Eshoo ; Steven A. Hofstadler ; David J. EckerSource :
- Annals of the New York Academy of Sciences [ 0077-8923 ] ; 2007.
Descripteurs français
- KwdFr :
- Contrôle des maladies contagieuses (), Humains, Maladies transmissibles émergentes (diagnostic), Maladies transmissibles émergentes (microbiologie), Maladies transmissibles émergentes (parasitologie), Maladies transmissibles émergentes (virologie), Maladies virales (diagnostic), Maladies virales (virologie), Réaction de polymérisation en chaîne (), Spectrométrie de masse ESI (), Virus (isolement et purification).
- MESH :
- diagnostic : Maladies transmissibles émergentes, Maladies virales.
- isolement et purification : Virus.
- microbiologie : Maladies transmissibles émergentes.
- parasitologie : Maladies transmissibles émergentes.
- virologie : Maladies transmissibles émergentes, Maladies virales.
- Contrôle des maladies contagieuses, Humains, Réaction de polymérisation en chaîne, Spectrométrie de masse ESI.
English descriptors
- KwdEn :
- Communicable Disease Control (methods), Communicable Diseases, Emerging (diagnosis), Communicable Diseases, Emerging (microbiology), Communicable Diseases, Emerging (parasitology), Communicable Diseases, Emerging (virology), Humans, Polymerase Chain Reaction (methods), Spectrometry, Mass, Electrospray Ionization (methods), Virus Diseases (diagnosis), Virus Diseases (virology), Viruses (isolation & purification).
- MESH :
- diagnosis : Communicable Diseases, Emerging, Virus Diseases.
- isolation & purification : Viruses.
- methods : Communicable Disease Control, Polymerase Chain Reaction, Spectrometry, Mass, Electrospray Ionization.
- microbiology : Communicable Diseases, Emerging.
- parasitology : Communicable Diseases, Emerging.
- virology : Communicable Diseases, Emerging, Virus Diseases.
- Humans.
Abstract
Newly emergent infectious diseases are a global public health problem. The population dense regions of Southeast Asia are the epicenter of many emerging diseases, as evidenced by the outbreak of Nipah, SARS, avian influenza (H5N1), Dengue, and enterovirus 71 in this region in the past decade. Rapid identification, epidemiologic surveillance, and mitigation of transmission are major challenges in ensuring public health safety. Here we describe a powerful new approach for infectious disease surveillance that is based on polymerase chain reaction (PCR) to amplify nucleic acid targets from large groupings of organisms, electrospray ionization mass spectrometry (ESI-MS) for accurate mass measurements of the PCR products, and base composition signature analysis to identify organisms in a sample. This approach is capable of automated analysis of more than 1,500 PCR reactions a day. It is applicable to the surveillance of bacterial, viral, fungal, or protozoal pathogens and will facilitate rapid characterization of known and emerging pathogens.
DOI: 10.1196/annals.1408.008
PubMed: 17470915
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pubmed:17470915Le document en format XML
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<front><div type="abstract" xml:lang="en">Newly emergent infectious diseases are a global public health problem. The population dense regions of Southeast Asia are the epicenter of many emerging diseases, as evidenced by the outbreak of Nipah, SARS, avian influenza (H5N1), Dengue, and enterovirus 71 in this region in the past decade. Rapid identification, epidemiologic surveillance, and mitigation of transmission are major challenges in ensuring public health safety. Here we describe a powerful new approach for infectious disease surveillance that is based on polymerase chain reaction (PCR) to amplify nucleic acid targets from large groupings of organisms, electrospray ionization mass spectrometry (ESI-MS) for accurate mass measurements of the PCR products, and base composition signature analysis to identify organisms in a sample. This approach is capable of automated analysis of more than 1,500 PCR reactions a day. It is applicable to the surveillance of bacterial, viral, fungal, or protozoal pathogens and will facilitate rapid characterization of known and emerging pathogens.</div>
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<Abstract><AbstractText>Newly emergent infectious diseases are a global public health problem. The population dense regions of Southeast Asia are the epicenter of many emerging diseases, as evidenced by the outbreak of Nipah, SARS, avian influenza (H5N1), Dengue, and enterovirus 71 in this region in the past decade. Rapid identification, epidemiologic surveillance, and mitigation of transmission are major challenges in ensuring public health safety. Here we describe a powerful new approach for infectious disease surveillance that is based on polymerase chain reaction (PCR) to amplify nucleic acid targets from large groupings of organisms, electrospray ionization mass spectrometry (ESI-MS) for accurate mass measurements of the PCR products, and base composition signature analysis to identify organisms in a sample. This approach is capable of automated analysis of more than 1,500 PCR reactions a day. It is applicable to the surveillance of bacterial, viral, fungal, or protozoal pathogens and will facilitate rapid characterization of known and emerging pathogens.</AbstractText>
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