Development of a red-shifted fluorescence-based assay for SARS-coronavirus 3CL protease: identification of a novel class of anti-SARS agents from the tropical marine sponge Axinella corrugata.
Identifieur interne : 001614 ( Ncbi/Merge ); précédent : 001613; suivant : 001615Development of a red-shifted fluorescence-based assay for SARS-coronavirus 3CL protease: identification of a novel class of anti-SARS agents from the tropical marine sponge Axinella corrugata.
Auteurs : Pamela Hamill [Canada] ; Derek Hudson ; Richard Y. Kao ; Polly Chow ; Meera Raj ; Hongyan Xu ; Martin J. Richer ; François JeanSource :
- Biological chemistry [ 1431-6730 ] ; 2006.
Descripteurs français
- KwdFr :
- Animaux, Antiviraux (), Antiviraux (pharmacologie), Cellules Vero, Cinétique, Cysteine endopeptidases (), Cysteine endopeptidases (isolement et purification), Facteurs temps, Inhibiteurs de protéases (), Inhibiteurs de protéases (pharmacologie), Ombelliférones (), Ombelliférones (pharmacologie), Porifera (), Prolifération cellulaire (), Protéines virales (), Protéines virales (antagonistes et inhibiteurs), Protéines virales (isolement et purification), Relation structure-activité, Réplication virale (), Réplication virale (physiologie), Sensibilité et spécificité, Spectrométrie de fluorescence (), Structure moléculaire, Virus du SRAS (), Virus du SRAS (enzymologie), Évaluation préclinique de médicament.
- MESH :
- antagonistes et inhibiteurs : Protéines virales.
- enzymologie : Virus du SRAS.
- isolement et purification : Cysteine endopeptidases, Protéines virales.
- pharmacologie : Antiviraux, Inhibiteurs de protéases, Ombelliférones.
- physiologie : Réplication virale.
- Animaux, Antiviraux, Cellules Vero, Cinétique, Cysteine endopeptidases, Facteurs temps, Inhibiteurs de protéases, Ombelliférones, Porifera, Prolifération cellulaire, Protéines virales, Relation structure-activité, Réplication virale, Sensibilité et spécificité, Spectrométrie de fluorescence, Structure moléculaire, Virus du SRAS, Évaluation préclinique de médicament.
English descriptors
- KwdEn :
- Animals, Antiviral Agents (chemistry), Antiviral Agents (pharmacology), Cell Proliferation (drug effects), Chlorocebus aethiops, Cysteine Endopeptidases (chemistry), Cysteine Endopeptidases (isolation & purification), Drug Evaluation, Preclinical, Kinetics, Molecular Structure, Porifera (chemistry), Porifera (classification), Protease Inhibitors (chemistry), Protease Inhibitors (classification), Protease Inhibitors (pharmacology), SARS Virus (drug effects), SARS Virus (enzymology), Sensitivity and Specificity, Spectrometry, Fluorescence (methods), Structure-Activity Relationship, Time Factors, Umbelliferones (chemistry), Umbelliferones (pharmacology), Vero Cells, Viral Proteins (antagonists & inhibitors), Viral Proteins (chemistry), Viral Proteins (isolation & purification), Virus Replication (drug effects), Virus Replication (physiology).
- MESH :
- chemical , antagonists & inhibitors : Viral Proteins.
- chemical , chemistry : Antiviral Agents, Cysteine Endopeptidases, Protease Inhibitors, Umbelliferones, Viral Proteins.
- chemical , classification : Protease Inhibitors.
- chemical , isolation & purification : Cysteine Endopeptidases, Viral Proteins.
- chemical , pharmacology : Antiviral Agents, Protease Inhibitors, Umbelliferones.
- chemistry : Porifera.
- classification : Porifera.
- drug effects : Cell Proliferation, SARS Virus, Virus Replication.
- enzymology : SARS Virus.
- methods : Spectrometry, Fluorescence.
- physiology : Virus Replication.
- Animals, Chlorocebus aethiops, Drug Evaluation, Preclinical, Kinetics, Molecular Structure, Sensitivity and Specificity, Structure-Activity Relationship, Time Factors, Vero Cells.
Abstract
SARS-coronavirus (SARS-CoV) encodes a main protease, 3CLpro, which plays an essential role in the viral life cycle and is currently the prime target for discovering new anti-coronavirus agents. In this article, we report our success in developing a novel red-shifted (RS) fluorescence-based assay for 3CLpro and its application for identifying small-molecule anti-SARS agents from marine organisms. We have synthesised and characterised the first generation of a red-shifted internally quenched fluorogenic substrate (RS-IQFS) for 3CLpro based on resonance energy transfer between the donor and acceptor pair CAL Fluor Red 610 and Black Hole Quencher-1 (Km and kcat values of 14 microM and 0.65 min-1). The RS-IQFS primary sequence was selected based on the results of our screening analysis of 3CLpro performed using a series of blue-shifted (BS)-IQFSs corresponding to the 3CLpro-mediated cleavage junctions of the SARS-CoV polyproteins. In contrast to BS-IQFSs, the RS-IQFS was not susceptible to fluorescence interference from coloured samples and allowed for successful screening of marine natural products and identification of a coumarin derivative, esculetin-4-carboxylic acid ethyl ester, a novel 3CLpro inhibitor (IC50=46 microM) and anti-SARS agent (EC50=112 microM; median toxic concentration>800 microM) from the tropical marine sponge Axinella corrugata.
DOI: 10.1515/BC.2006.131
PubMed: 16895476
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 002132
- to stream PubMed, to step Curation: 002132
- to stream PubMed, to step Checkpoint: 002226
Links to Exploration step
pubmed:16895476Le document en format XML
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Kao</name></author><author><name sortKey="Chow, Polly" sort="Chow, Polly" uniqKey="Chow P" first="Polly" last="Chow">Polly Chow</name></author><author><name sortKey="Raj, Meera" sort="Raj, Meera" uniqKey="Raj M" first="Meera" last="Raj">Meera Raj</name></author><author><name sortKey="Xu, Hongyan" sort="Xu, Hongyan" uniqKey="Xu H" first="Hongyan" last="Xu">Hongyan Xu</name></author><author><name sortKey="Richer, Martin J" sort="Richer, Martin J" uniqKey="Richer M" first="Martin J" last="Richer">Martin J. Richer</name></author><author><name sortKey="Jean, Francois" sort="Jean, Francois" uniqKey="Jean F" first="François" last="Jean">François Jean</name></author></analytic><series><title level="j">Biological chemistry</title><idno type="ISSN">1431-6730</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antiviral Agents (chemistry)</term><term>Antiviral Agents (pharmacology)</term><term>Cell Proliferation (drug effects)</term><term>Chlorocebus aethiops</term><term>Cysteine Endopeptidases (chemistry)</term><term>Cysteine Endopeptidases (isolation & purification)</term><term>Drug Evaluation, Preclinical</term><term>Kinetics</term><term>Molecular Structure</term><term>Porifera (chemistry)</term><term>Porifera (classification)</term><term>Protease Inhibitors (chemistry)</term><term>Protease Inhibitors (classification)</term><term>Protease Inhibitors (pharmacology)</term><term>SARS Virus (drug effects)</term><term>SARS Virus (enzymology)</term><term>Sensitivity and Specificity</term><term>Spectrometry, Fluorescence (methods)</term><term>Structure-Activity Relationship</term><term>Time Factors</term><term>Umbelliferones (chemistry)</term><term>Umbelliferones (pharmacology)</term><term>Vero Cells</term><term>Viral Proteins (antagonists & inhibitors)</term><term>Viral Proteins (chemistry)</term><term>Viral Proteins (isolation & purification)</term><term>Virus Replication (drug effects)</term><term>Virus Replication (physiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Antiviraux ()</term><term>Antiviraux (pharmacologie)</term><term>Cellules Vero</term><term>Cinétique</term><term>Cysteine endopeptidases ()</term><term>Cysteine endopeptidases (isolement et purification)</term><term>Facteurs temps</term><term>Inhibiteurs de protéases ()</term><term>Inhibiteurs de protéases (pharmacologie)</term><term>Ombelliférones ()</term><term>Ombelliférones (pharmacologie)</term><term>Porifera ()</term><term>Prolifération cellulaire ()</term><term>Protéines virales ()</term><term>Protéines virales (antagonistes et inhibiteurs)</term><term>Protéines virales (isolement et purification)</term><term>Relation structure-activité</term><term>Réplication virale ()</term><term>Réplication virale (physiologie)</term><term>Sensibilité et spécificité</term><term>Spectrométrie de fluorescence ()</term><term>Structure moléculaire</term><term>Virus du SRAS ()</term><term>Virus du SRAS (enzymologie)</term><term>Évaluation préclinique de médicament</term></keywords><keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Antiviral Agents</term><term>Cysteine Endopeptidases</term><term>Protease Inhibitors</term><term>Umbelliferones</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="classification" xml:lang="en"><term>Protease Inhibitors</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Cysteine Endopeptidases</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Antiviral Agents</term><term>Protease Inhibitors</term><term>Umbelliferones</term></keywords><keywords scheme="MESH" qualifier="antagonistes et inhibiteurs" xml:lang="fr"><term>Protéines virales</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>Porifera</term></keywords><keywords scheme="MESH" qualifier="classification" xml:lang="en"><term>Porifera</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>Cell Proliferation</term><term>SARS Virus</term><term>Virus Replication</term></keywords><keywords scheme="MESH" qualifier="enzymologie" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="enzymology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Cysteine endopeptidases</term><term>Protéines virales</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Spectrometry, Fluorescence</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antiviraux</term><term>Inhibiteurs de protéases</term><term>Ombelliférones</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Réplication virale</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Virus Replication</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Drug Evaluation, Preclinical</term><term>Kinetics</term><term>Molecular Structure</term><term>Sensitivity and Specificity</term><term>Structure-Activity Relationship</term><term>Time Factors</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Antiviraux</term><term>Cellules Vero</term><term>Cinétique</term><term>Cysteine endopeptidases</term><term>Facteurs temps</term><term>Inhibiteurs de protéases</term><term>Ombelliférones</term><term>Porifera</term><term>Prolifération cellulaire</term><term>Protéines virales</term><term>Relation structure-activité</term><term>Réplication virale</term><term>Sensibilité et spécificité</term><term>Spectrométrie de fluorescence</term><term>Structure moléculaire</term><term>Virus du SRAS</term><term>Évaluation préclinique de médicament</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">SARS-coronavirus (SARS-CoV) encodes a main protease, 3CLpro, which plays an essential role in the viral life cycle and is currently the prime target for discovering new anti-coronavirus agents. In this article, we report our success in developing a novel red-shifted (RS) fluorescence-based assay for 3CLpro and its application for identifying small-molecule anti-SARS agents from marine organisms. We have synthesised and characterised the first generation of a red-shifted internally quenched fluorogenic substrate (RS-IQFS) for 3CLpro based on resonance energy transfer between the donor and acceptor pair CAL Fluor Red 610 and Black Hole Quencher-1 (Km and kcat values of 14 microM and 0.65 min-1). The RS-IQFS primary sequence was selected based on the results of our screening analysis of 3CLpro performed using a series of blue-shifted (BS)-IQFSs corresponding to the 3CLpro-mediated cleavage junctions of the SARS-CoV polyproteins. In contrast to BS-IQFSs, the RS-IQFS was not susceptible to fluorescence interference from coloured samples and allowed for successful screening of marine natural products and identification of a coumarin derivative, esculetin-4-carboxylic acid ethyl ester, a novel 3CLpro inhibitor (IC50=46 microM) and anti-SARS agent (EC50=112 microM; median toxic concentration>800 microM) from the tropical marine sponge Axinella corrugata.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16895476</PMID><DateCompleted><Year>2006</Year><Month>12</Month><Day>12</Day></DateCompleted><DateRevised><Year>2020</Year><Month>03</Month><Day>25</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1431-6730</ISSN><JournalIssue CitedMedium="Print"><Volume>387</Volume><Issue>8</Issue><PubDate><Year>2006</Year><Month>Aug</Month></PubDate></JournalIssue><Title>Biological chemistry</Title><ISOAbbreviation>Biol. Chem.</ISOAbbreviation></Journal><ArticleTitle>Development of a red-shifted fluorescence-based assay for SARS-coronavirus 3CL protease: identification of a novel class of anti-SARS agents from the tropical marine sponge Axinella corrugata.</ArticleTitle><Pagination><MedlinePgn>1063-74</MedlinePgn></Pagination><Abstract><AbstractText>SARS-coronavirus (SARS-CoV) encodes a main protease, 3CLpro, which plays an essential role in the viral life cycle and is currently the prime target for discovering new anti-coronavirus agents. In this article, we report our success in developing a novel red-shifted (RS) fluorescence-based assay for 3CLpro and its application for identifying small-molecule anti-SARS agents from marine organisms. We have synthesised and characterised the first generation of a red-shifted internally quenched fluorogenic substrate (RS-IQFS) for 3CLpro based on resonance energy transfer between the donor and acceptor pair CAL Fluor Red 610 and Black Hole Quencher-1 (Km and kcat values of 14 microM and 0.65 min-1). The RS-IQFS primary sequence was selected based on the results of our screening analysis of 3CLpro performed using a series of blue-shifted (BS)-IQFSs corresponding to the 3CLpro-mediated cleavage junctions of the SARS-CoV polyproteins. In contrast to BS-IQFSs, the RS-IQFS was not susceptible to fluorescence interference from coloured samples and allowed for successful screening of marine natural products and identification of a coumarin derivative, esculetin-4-carboxylic acid ethyl ester, a novel 3CLpro inhibitor (IC50=46 microM) and anti-SARS agent (EC50=112 microM; median toxic concentration>800 microM) from the tropical marine sponge Axinella corrugata.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Hamill</LastName><ForeName>Pamela</ForeName><Initials>P</Initials><AffiliationInfo><Affiliation>Department of Microbiology and Immunology, Life Sciences Centre, University of British Columbia, 3559-2350 Health Sciences Mall, Vancouver, BC V6T 1Z3, Canada.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Hudson</LastName><ForeName>Derek</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Kao</LastName><ForeName>Richard Y</ForeName><Initials>RY</Initials></Author><Author ValidYN="Y"><LastName>Chow</LastName><ForeName>Polly</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Raj</LastName><ForeName>Meera</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Hongyan</ForeName><Initials>H</Initials></Author><Author ValidYN="Y"><LastName>Richer</LastName><ForeName>Martin J</ForeName><Initials>MJ</Initials></Author><Author ValidYN="Y"><LastName>Jean</LastName><ForeName>François</ForeName><Initials>F</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>Germany</Country><MedlineTA>Biol Chem</MedlineTA><NlmUniqueID>9700112</NlmUniqueID><ISSNLinking>1431-6730</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000998">Antiviral Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011480">Protease Inhibitors</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014468">Umbelliferones</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014764">Viral Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C515382">esculetin-4-carboxylic acid ethyl ester</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.4.22.-</RegistryNumber><NameOfSubstance UI="C099456">3C-like proteinase, Coronavirus</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.4.22.-</RegistryNumber><NameOfSubstance UI="D003546">Cysteine Endopeptidases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><CommentsCorrectionsList><CommentsCorrections RefType="CommentIn"><RefSource>Biol Chem. 2007 Sep;388(9):875</RefSource><PMID Version="1">17696769</PMID></CommentsCorrections></CommentsCorrectionsList><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000998" MajorTopicYN="N">Antiviral Agents</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D049109" MajorTopicYN="N">Cell Proliferation</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D002522" MajorTopicYN="N">Chlorocebus aethiops</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003546" MajorTopicYN="N">Cysteine Endopeptidases</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D004353" MajorTopicYN="N">Drug Evaluation, Preclinical</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007700" MajorTopicYN="N">Kinetics</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015394" MajorTopicYN="N">Molecular Structure</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011161" MajorTopicYN="N">Porifera</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000145" MajorTopicYN="Y">classification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011480" MajorTopicYN="N">Protease Inhibitors</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000145" MajorTopicYN="N">classification</QualifierName><QualifierName UI="Q000494" MajorTopicYN="N">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000201" MajorTopicYN="Y">enzymology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013050" MajorTopicYN="N">Spectrometry, Fluorescence</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013329" MajorTopicYN="N">Structure-Activity Relationship</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013997" MajorTopicYN="N">Time Factors</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014468" MajorTopicYN="N">Umbelliferones</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="N">chemistry</QualifierName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014709" MajorTopicYN="N">Vero Cells</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014764" MajorTopicYN="N">Viral Proteins</DescriptorName><QualifierName UI="Q000037" MajorTopicYN="N">antagonists & inhibitors</QualifierName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014779" MajorTopicYN="N">Virus Replication</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="N">drug effects</QualifierName><QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2006</Year><Month>8</Month><Day>10</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2006</Year><Month>12</Month><Day>13</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2006</Year><Month>8</Month><Day>10</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16895476</ArticleId><ArticleId IdType="doi">10.1515/BC.2006.131</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>Canada</li></country></list><tree><noCountry><name sortKey="Chow, Polly" sort="Chow, Polly" uniqKey="Chow P" first="Polly" last="Chow">Polly Chow</name><name sortKey="Hudson, Derek" sort="Hudson, Derek" uniqKey="Hudson D" first="Derek" last="Hudson">Derek Hudson</name><name sortKey="Jean, Francois" sort="Jean, Francois" uniqKey="Jean F" first="François" last="Jean">François Jean</name><name sortKey="Kao, Richard Y" sort="Kao, Richard Y" uniqKey="Kao R" first="Richard Y" last="Kao">Richard Y. Kao</name><name sortKey="Raj, Meera" sort="Raj, Meera" uniqKey="Raj M" first="Meera" last="Raj">Meera Raj</name><name sortKey="Richer, Martin J" sort="Richer, Martin J" uniqKey="Richer M" first="Martin J" last="Richer">Martin J. Richer</name><name sortKey="Xu, Hongyan" sort="Xu, Hongyan" uniqKey="Xu H" first="Hongyan" last="Xu">Hongyan Xu</name></noCountry><country name="Canada"><noRegion><name sortKey="Hamill, Pamela" sort="Hamill, Pamela" uniqKey="Hamill P" first="Pamela" last="Hamill">Pamela Hamill</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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|type= RBID
|clé= pubmed:16895476
|texte= Development of a red-shifted fluorescence-based assay for SARS-coronavirus 3CL protease: identification of a novel class of anti-SARS agents from the tropical marine sponge Axinella corrugata.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/RBID.i -Sk "pubmed:16895476" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |