Long-lived effector/central memory T-cell responses to severe acute respiratory syndrome coronavirus (SARS-CoV) S antigen in recovered SARS patients
Identifieur interne : 001540 ( Ncbi/Merge ); précédent : 001539; suivant : 001541Long-lived effector/central memory T-cell responses to severe acute respiratory syndrome coronavirus (SARS-CoV) S antigen in recovered SARS patients
Auteurs : Li-Tao Yang [République populaire de Chine] ; Hui Peng [République populaire de Chine] ; Zhao-Ling Zhu [République populaire de Chine] ; Gang Li [République populaire de Chine] ; Zi-Tong Huang [République populaire de Chine] ; Zhi-Xin Zhao [République populaire de Chine] ; Richard A. Koup [États-Unis] ; Robert T. Bailer [États-Unis] ; Chang-You Wu [République populaire de Chine]Source :
- Clinical Immunology (Orlando, Fla.) [ 1521-6616 ] ; 2006.
Abstract
The role of cell-mediated immunity in human SARS-CoV infection is still not well understood. In this study, we found that memory T-cell responses against the spike (S) protein were persistent for more than 1 year after SARS-CoV infection by detecting the production of IFN-γ using ELISA and ELISpot assays. Flow cytometric analysis showed that both CD4+ and CD8+ T cells were involved in cellular responses against SARS-CoV infection. Interestingly, most of SARS-CoV S-specific memory CD4+ T cells were central memory cells expressing CD45RO+ CCR7+ CD62L−. However, the majority of memory CD8+ T cells revealed effector memory phenotype expressing CD45RO− CCR7− CD62L−. Thus, our study provides the evidence that SARS-CoV infection in humans can induce cellular immune response that is persistent for a long period of time. These data may have an important implication in the possibility of designing effective vaccine against SARS-CoV infection, specifically in defining T-cell populations that are implicated in protective immunity.
Url:
DOI: 10.1016/j.clim.2006.05.002
PubMed: 16781892
PubMed Central: 7106132
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to severe acute respiratory syndrome coronavirus (SARS-CoV) S antigen in recovered SARS patients</title><author><name sortKey="Yang, Li Tao" sort="Yang, Li Tao" uniqKey="Yang L" first="Li-Tao" last="Yang">Li-Tao Yang</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Immunology, Zhongshan Medical School, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou 510080, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Immunology, Zhongshan Medical School, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou 510080</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Peng, Hui" sort="Peng, Hui" uniqKey="Peng H" first="Hui" last="Peng">Hui Peng</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Immunology, Zhongshan Medical School, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou 510080, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Immunology, Zhongshan Medical School, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou 510080</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Zhu, Zhao Ling" sort="Zhu, Zhao Ling" uniqKey="Zhu Z" first="Zhao-Ling" last="Zhu">Zhao-Ling Zhu</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Immunology, Zhongshan Medical School, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou 510080, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Immunology, Zhongshan Medical School, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou 510080</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Li, Gang" sort="Li, Gang" uniqKey="Li G" first="Gang" last="Li">Gang Li</name><affiliation wicri:level="1"><nlm:aff id="aff2">Third affiliated hospital of the Sun Yat-sen University, Guangzhou 510630, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Third affiliated hospital of the Sun Yat-sen University, Guangzhou 510630</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Huang, Zi Tong" sort="Huang, Zi Tong" uniqKey="Huang Z" first="Zi-Tong" last="Huang">Zi-Tong Huang</name><affiliation wicri:level="1"><nlm:aff id="aff3">Second affiliated hospital of the Sun Yat-sen University, Guangzhou 510120, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Second affiliated hospital of the Sun Yat-sen University, Guangzhou 510120</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Zhao, Zhi Xin" sort="Zhao, Zhi Xin" uniqKey="Zhao Z" first="Zhi-Xin" last="Zhao">Zhi-Xin Zhao</name><affiliation wicri:level="1"><nlm:aff id="aff2">Third affiliated hospital of the Sun Yat-sen University, Guangzhou 510630, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Third affiliated hospital of the Sun Yat-sen University, Guangzhou 510630</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Koup, Richard A" sort="Koup, Richard A" uniqKey="Koup R" first="Richard A." last="Koup">Richard A. Koup</name><affiliation wicri:level="2"><nlm:aff id="aff4">Vaccine Research Center, NIAID/NIH, Bethesda, MD 20892, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Vaccine Research Center, NIAID/NIH, Bethesda, MD 20892</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Bailer, Robert T" sort="Bailer, Robert T" uniqKey="Bailer R" first="Robert T." last="Bailer">Robert T. Bailer</name><affiliation wicri:level="2"><nlm:aff id="aff4">Vaccine Research Center, NIAID/NIH, Bethesda, MD 20892, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Vaccine Research Center, NIAID/NIH, Bethesda, MD 20892</wicri:regionArea><placeName><region type="state">Maryland</region></placeName></affiliation></author><author><name sortKey="Wu, Chang You" sort="Wu, Chang You" uniqKey="Wu C" first="Chang-You" last="Wu">Chang-You Wu</name><affiliation wicri:level="1"><nlm:aff id="aff1">Department of Immunology, Zhongshan Medical School, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou 510080, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Immunology, Zhongshan Medical School, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou 510080</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author></analytic><series><title level="j">Clinical Immunology (Orlando, Fla.)</title><idno type="ISSN">1521-6616</idno><idno type="eISSN">1521-7035</idno><imprint><date when="2006">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The role of cell-mediated immunity in human SARS-CoV infection is still not well understood. In this study, we found that memory T-cell responses against the spike (S) protein were persistent for more than 1 year after SARS-CoV infection by detecting the production of IFN-γ using ELISA and ELISpot assays. Flow cytometric analysis showed that both CD4<sup>+</sup> and CD8<sup>+</sup> T cells were involved in cellular responses against SARS-CoV infection. Interestingly, most of SARS-CoV S-specific memory CD4<sup>+</sup> T cells were central memory cells expressing CD45RO<sup>+</sup> CCR7<sup>+</sup> CD62L<sup>−</sup>. However, the majority of memory CD8<sup>+</sup> T cells revealed effector memory phenotype expressing CD45RO<sup>−</sup> CCR7<sup>−</sup> CD62L<sup>−</sup>. Thus, our study provides the evidence that SARS-CoV infection in humans can induce cellular immune response that is persistent for a long period of time. 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Clin Immunol</journal-id><journal-id journal-id-type="iso-abbrev">Clin. Immunol</journal-id><journal-title-group><journal-title>Clinical Immunology (Orlando, Fla.)</journal-title></journal-title-group><issn pub-type="ppub">1521-6616</issn><issn pub-type="epub">1521-7035</issn><publisher><publisher-name>Elsevier Inc.</publisher-name></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">16781892</article-id><article-id pub-id-type="pmc">7106132</article-id><article-id pub-id-type="publisher-id">S1521-6616(06)00735-2</article-id><article-id pub-id-type="doi">10.1016/j.clim.2006.05.002</article-id><article-categories><subj-group subj-group-type="heading"><subject>Article</subject></subj-group></article-categories><title-group><article-title>Long-lived effector/central memory T-cell responses to severe acute respiratory syndrome coronavirus (SARS-CoV) S antigen in recovered SARS patients</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Yang</surname><given-names>Li-Tao</given-names></name><xref rid="aff1" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Peng</surname><given-names>Hui</given-names></name><xref rid="aff1" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Zhu</surname><given-names>Zhao-Ling</given-names></name><xref rid="aff1" ref-type="aff">a</xref></contrib><contrib contrib-type="author"><name><surname>Li</surname><given-names>Gang</given-names></name><xref rid="aff2" ref-type="aff">b</xref></contrib><contrib contrib-type="author"><name><surname>Huang</surname><given-names>Zi-Tong</given-names></name><xref rid="aff3" ref-type="aff">c</xref></contrib><contrib contrib-type="author"><name><surname>Zhao</surname><given-names>Zhi-Xin</given-names></name><xref rid="aff2" ref-type="aff">b</xref></contrib><contrib contrib-type="author"><name><surname>Koup</surname><given-names>Richard A.</given-names></name><xref rid="aff4" ref-type="aff">d</xref></contrib><contrib contrib-type="author"><name><surname>Bailer</surname><given-names>Robert T.</given-names></name><xref rid="aff4" ref-type="aff">d</xref></contrib><contrib contrib-type="author"><name><surname>Wu</surname><given-names>Chang-You</given-names></name><email>Changyou_wu@yahoo.com</email><xref rid="aff1" ref-type="aff">a</xref><xref rid="cor1" ref-type="corresp">⁎</xref></contrib></contrib-group><aff id="aff1"><label>a</label>Department of Immunology, Zhongshan Medical School, Sun Yat-sen University, 74 Zhongshan 2nd Road, Guangzhou 510080, China</aff><aff id="aff2"><label>b</label>Third affiliated hospital of the Sun Yat-sen University, Guangzhou 510630, China</aff><aff id="aff3"><label>c</label>Second affiliated hospital of the Sun Yat-sen University, Guangzhou 510120, China</aff><aff id="aff4"><label>d</label>Vaccine Research Center, NIAID/NIH, Bethesda, MD 20892, USA</aff><author-notes><corresp id="cor1"><label>⁎</label>Corresponding author. Fax: +86 20 87331552. <email>Changyou_wu@yahoo.com</email></corresp></author-notes><pub-date pub-type="pmc-release"><day>16</day><month>6</month><year>2006</year></pub-date><pmc-comment> PMC Release delay is 0 months and 0 days and was based on .</pmc-comment>
<pub-date pub-type="ppub"><month>8</month><year>2006</year></pub-date><pub-date pub-type="epub"><day>16</day><month>6</month><year>2006</year></pub-date><volume>120</volume><issue>2</issue><fpage>171</fpage><lpage>178</lpage><history><date date-type="received"><day>22</day><month>11</month><year>2005</year></date><date date-type="rev-recd"><day>21</day><month>4</month><year>2006</year></date><date date-type="accepted"><day>1</day><month>5</month><year>2006</year></date></history><permissions><copyright-statement>Copyright © 2006 Elsevier Inc. All rights reserved.</copyright-statement><copyright-year>2006</copyright-year><copyright-holder>Elsevier Inc.</copyright-holder><license><license-p>Since January 2020 Elsevier has created a COVID-19 resource centre with free information in English and Mandarin on the novel coronavirus COVID-19. The COVID-19 resource centre is hosted on Elsevier Connect, the company's public news and information website. Elsevier hereby grants permission to make all its COVID-19-related research that is available on the COVID-19 resource centre - including this research content - immediately available in PubMed Central and other publicly funded repositories, such as the WHO COVID database with rights for unrestricted research re-use and analyses in any form or by any means with acknowledgement of the original source. These permissions are granted for free by Elsevier for as long as the COVID-19 resource centre remains active.</license-p></license></permissions><abstract><p>The role of cell-mediated immunity in human SARS-CoV infection is still not well understood. In this study, we found that memory T-cell responses against the spike (S) protein were persistent for more than 1 year after SARS-CoV infection by detecting the production of IFN-γ using ELISA and ELISpot assays. Flow cytometric analysis showed that both CD4<sup>+</sup> and CD8<sup>+</sup> T cells were involved in cellular responses against SARS-CoV infection. Interestingly, most of SARS-CoV S-specific memory CD4<sup>+</sup> T cells were central memory cells expressing CD45RO<sup>+</sup> CCR7<sup>+</sup> CD62L<sup>−</sup>. However, the majority of memory CD8<sup>+</sup> T cells revealed effector memory phenotype expressing CD45RO<sup>−</sup> CCR7<sup>−</sup> CD62L<sup>−</sup>. Thus, our study provides the evidence that SARS-CoV infection in humans can induce cellular immune response that is persistent for a long period of time. These data may have an important implication in the possibility of designing effective vaccine against SARS-CoV infection, specifically in defining T-cell populations that are implicated in protective immunity.</p></abstract><kwd-group><title>Keywords</title><kwd>SARS</kwd><kwd>SARS-CoV</kwd><kwd>S protein</kwd><kwd>IFN-γ</kwd><kwd>Cellular immune response</kwd><kwd>Memory T cells</kwd></kwd-group></article-meta></front></pmc><affiliations><list><country><li>République populaire de Chine</li><li>États-Unis</li></country><region><li>Guangdong</li><li>Maryland</li></region><settlement><li>Jiangmen</li></settlement></list><tree><country name="République populaire de Chine"><region name="Guangdong"><name sortKey="Yang, Li Tao" sort="Yang, Li Tao" uniqKey="Yang L" first="Li-Tao" last="Yang">Li-Tao Yang</name></region><name sortKey="Huang, Zi Tong" sort="Huang, Zi Tong" uniqKey="Huang Z" first="Zi-Tong" last="Huang">Zi-Tong Huang</name><name sortKey="Li, Gang" sort="Li, Gang" uniqKey="Li G" first="Gang" last="Li">Gang Li</name><name sortKey="Peng, Hui" sort="Peng, Hui" uniqKey="Peng H" first="Hui" last="Peng">Hui Peng</name><name sortKey="Wu, Chang You" sort="Wu, Chang You" uniqKey="Wu C" first="Chang-You" last="Wu">Chang-You Wu</name><name sortKey="Zhao, Zhi Xin" sort="Zhao, Zhi Xin" uniqKey="Zhao Z" first="Zhi-Xin" last="Zhao">Zhi-Xin Zhao</name><name sortKey="Zhu, Zhao Ling" sort="Zhu, Zhao Ling" uniqKey="Zhu Z" first="Zhao-Ling" last="Zhu">Zhao-Ling Zhu</name></country><country name="États-Unis"><region name="Maryland"><name sortKey="Koup, Richard A" sort="Koup, Richard A" uniqKey="Koup R" first="Richard A." last="Koup">Richard A. Koup</name></region><name sortKey="Bailer, Robert T" sort="Bailer, Robert T" uniqKey="Bailer R" first="Robert T." last="Bailer">Robert T. Bailer</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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