SrasV1, Ncbi, Merge, bibRecord, 001337

Important role for the transmembrane domain of severe acute respiratory syndrome coronavirus spike protein during entry.

Identifieur interne : 001337 ( Ncbi/Merge ); précédent : 001336; suivant : 001338

Important role for the transmembrane domain of severe acute respiratory syndrome coronavirus spike protein during entry.

Auteurs : Rene Broer [Pays-Bas] ; Bertrand Boson ; Willy Spaan ; François-Loïc Cosset ; Jeroen Corver

Source :

Journal of virology [ 0022-538X ] ; 2006.

RBID : pubmed:16415007

Descripteurs français

English descriptors

KwdEn :
- Amino Acid Sequence, Animals, Base Sequence, Cell Line, Chlorocebus aethiops, DNA, Viral (genetics), Humans, Luciferases, Renilla (genetics), Membrane Fusion (physiology), Membrane Glycoproteins (chemistry), Membrane Glycoproteins (genetics), Membrane Glycoproteins (physiology), Molecular Sequence Data, Protein Structure, Quaternary, Protein Structure, Tertiary, Receptors, Virus (genetics), Recombinant Fusion Proteins (genetics), Recombinant Fusion Proteins (physiology), SARS Virus (genetics), SARS Virus (pathogenicity), SARS Virus (physiology), Spike Glycoprotein, Coronavirus, Vero Cells, Viral Envelope Proteins (chemistry), Viral Envelope Proteins (genetics), Viral Envelope Proteins (physiology).
MESH :
- chemical , chemistry : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , genetics : DNA, Viral, Luciferases, Renilla, Membrane Glycoproteins, Receptors, Virus, Recombinant Fusion Proteins, Viral Envelope Proteins.
- genetics : SARS Virus.
- pathogenicity : SARS Virus.
- physiology : Membrane Fusion, Membrane Glycoproteins, Recombinant Fusion Proteins, SARS Virus, Viral Envelope Proteins.
- Amino Acid Sequence, Animals, Base Sequence, Cell Line, Chlorocebus aethiops, Humans, Molecular Sequence Data, Protein Structure, Quaternary, Protein Structure, Tertiary, Spike Glycoprotein, Coronavirus, Vero Cells.

Abstract

The spike protein (S) of severe acute respiratory syndrome coronavirus (SARS-CoV) is responsible for receptor binding and membrane fusion. It contains a highly conserved transmembrane domain that consists of three parts: an N-terminal tryptophan-rich domain, a central domain, and a cysteine-rich C-terminal domain. The cytoplasmic tail of S has previously been shown to be required for assembly. Here, the roles of the transmembrane and cytoplasmic domains of S in the infectivity and membrane fusion activity of SARS-CoV have been studied. SARS-CoV S-pseudotyped retrovirus (SARSpp) was used to measure S-mediated infectivity. In addition, the cell-cell fusion activity of S was monitored by a Renilla luciferase-based cell-cell fusion assay. S(VSV-Cyt), an S chimera with a cytoplasmic tail derived from vesicular stomatitis virus G protein (VSV-G), and S(MHV-TMDCyt), an S chimera with the cytoplasmic and transmembrane domains of mouse hepatitis virus, displayed wild-type-like activity in both assays. S(VSV-TMDCyt), a chimera with the cytoplasmic and transmembrane domains of VSV-G, was impaired in the SARSpp and cell-cell fusion assays, showing 3 to 25% activity compared to the wild type, depending on the assay and the cells used. Examination of the oligomeric state of the chimeric S proteins in SARSpp revealed that S(VSV-TMDCyt) trimers were less stable than wild-type S trimers, possibly explaining the lowered fusogenicity and infectivity.

DOI: 10.1128/JVI.80.3.1302-1310.2006
PubMed: 16415007

Links toward previous steps (curation, corpus...)

to stream PubMed, to step Corpus: 002379
to stream PubMed, to step Curation: 002379
to stream PubMed, to step Checkpoint: 002147

Links to Exploration step

pubmed:16415007

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Important role for the transmembrane domain of severe acute respiratory syndrome coronavirus spike protein during entry.</title>
<author><name sortKey="Broer, Rene" sort="Broer, Rene" uniqKey="Broer R" first="Rene" last="Broer">Rene Broer</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Medical Microbiology, E4-P, Leiden University Medical Center, P.O. Box 9600, 2300 RC Leiden, The Netherlands.</nlm:affiliation>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea>Department of Medical Microbiology, E4-P, Leiden University Medical Center, P.O. Box 9600, 2300 RC Leiden</wicri:regionArea>
<wicri:noRegion>2300 RC Leiden</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Boson, Bertrand" sort="Boson, Bertrand" uniqKey="Boson B" first="Bertrand" last="Boson">Bertrand Boson</name>
</author>
<author><name sortKey="Spaan, Willy" sort="Spaan, Willy" uniqKey="Spaan W" first="Willy" last="Spaan">Willy Spaan</name>
</author>
<author><name sortKey="Cosset, Francois Loic" sort="Cosset, Francois Loic" uniqKey="Cosset F" first="François-Loïc" last="Cosset">François-Loïc Cosset</name>
</author>
<author><name sortKey="Corver, Jeroen" sort="Corver, Jeroen" uniqKey="Corver J" first="Jeroen" last="Corver">Jeroen Corver</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2006">2006</date>
<idno type="RBID">pubmed:16415007</idno>
<idno type="pmid">16415007</idno>
<idno type="doi">10.1128/JVI.80.3.1302-1310.2006</idno>
<idno type="wicri:Area/PubMed/Corpus">002379</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002379</idno>
<idno type="wicri:Area/PubMed/Curation">002379</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002379</idno>
<idno type="wicri:Area/PubMed/Checkpoint">002147</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002147</idno>
<idno type="wicri:Area/Ncbi/Merge">001337</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Important role for the transmembrane domain of severe acute respiratory syndrome coronavirus spike protein during entry.</title>
<author><name sortKey="Broer, Rene" sort="Broer, Rene" uniqKey="Broer R" first="Rene" last="Broer">Rene Broer</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Medical Microbiology, E4-P, Leiden University Medical Center, P.O. Box 9600, 2300 RC Leiden, The Netherlands.</nlm:affiliation>
<country xml:lang="fr">Pays-Bas</country>
<wicri:regionArea>Department of Medical Microbiology, E4-P, Leiden University Medical Center, P.O. Box 9600, 2300 RC Leiden</wicri:regionArea>
<wicri:noRegion>2300 RC Leiden</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Boson, Bertrand" sort="Boson, Bertrand" uniqKey="Boson B" first="Bertrand" last="Boson">Bertrand Boson</name>
</author>
<author><name sortKey="Spaan, Willy" sort="Spaan, Willy" uniqKey="Spaan W" first="Willy" last="Spaan">Willy Spaan</name>
</author>
<author><name sortKey="Cosset, Francois Loic" sort="Cosset, Francois Loic" uniqKey="Cosset F" first="François-Loïc" last="Cosset">François-Loïc Cosset</name>
</author>
<author><name sortKey="Corver, Jeroen" sort="Corver, Jeroen" uniqKey="Corver J" first="Jeroen" last="Corver">Jeroen Corver</name>
</author>
</analytic>
<series><title level="j">Journal of virology</title>
<idno type="ISSN">0022-538X</idno>
<imprint><date when="2006" type="published">2006</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Base Sequence</term>
<term>Cell Line</term>
<term>Chlorocebus aethiops</term>
<term>DNA, Viral (genetics)</term>
<term>Humans</term>
<term>Luciferases, Renilla (genetics)</term>
<term>Membrane Fusion (physiology)</term>
<term>Membrane Glycoproteins (chemistry)</term>
<term>Membrane Glycoproteins (genetics)</term>
<term>Membrane Glycoproteins (physiology)</term>
<term>Molecular Sequence Data</term>
<term>Protein Structure, Quaternary</term>
<term>Protein Structure, Tertiary</term>
<term>Receptors, Virus (genetics)</term>
<term>Recombinant Fusion Proteins (genetics)</term>
<term>Recombinant Fusion Proteins (physiology)</term>
<term>SARS Virus (genetics)</term>
<term>SARS Virus (pathogenicity)</term>
<term>SARS Virus (physiology)</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Vero Cells</term>
<term>Viral Envelope Proteins (chemistry)</term>
<term>Viral Envelope Proteins (genetics)</term>
<term>Viral Envelope Proteins (physiology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ADN viral (génétique)</term>
<term>Animaux</term>
<term>Cellules Vero</term>
<term>Données de séquences moléculaires</term>
<term>Fusion membranaire (physiologie)</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires ()</term>
<term>Glycoprotéines membranaires (génétique)</term>
<term>Glycoprotéines membranaires (physiologie)</term>
<term>Humains</term>
<term>Lignée cellulaire</term>
<term>Luciférases de Renilla (génétique)</term>
<term>Protéines de fusion recombinantes (génétique)</term>
<term>Protéines de fusion recombinantes (physiologie)</term>
<term>Protéines de l'enveloppe virale ()</term>
<term>Protéines de l'enveloppe virale (génétique)</term>
<term>Protéines de l'enveloppe virale (physiologie)</term>
<term>Récepteurs viraux (génétique)</term>
<term>Structure quaternaire des protéines</term>
<term>Structure tertiaire des protéines</term>
<term>Séquence d'acides aminés</term>
<term>Séquence nucléotidique</term>
<term>Virus du SRAS (génétique)</term>
<term>Virus du SRAS (pathogénicité)</term>
<term>Virus du SRAS (physiologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Membrane Glycoproteins</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Viral</term>
<term>Luciferases, Renilla</term>
<term>Membrane Glycoproteins</term>
<term>Receptors, Virus</term>
<term>Recombinant Fusion Proteins</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN viral</term>
<term>Glycoprotéines membranaires</term>
<term>Luciférases de Renilla</term>
<term>Protéines de fusion recombinantes</term>
<term>Protéines de l'enveloppe virale</term>
<term>Récepteurs viraux</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Fusion membranaire</term>
<term>Glycoprotéines membranaires</term>
<term>Protéines de fusion recombinantes</term>
<term>Protéines de l'enveloppe virale</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>Membrane Fusion</term>
<term>Membrane Glycoproteins</term>
<term>Recombinant Fusion Proteins</term>
<term>SARS Virus</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Base Sequence</term>
<term>Cell Line</term>
<term>Chlorocebus aethiops</term>
<term>Humans</term>
<term>Molecular Sequence Data</term>
<term>Protein Structure, Quaternary</term>
<term>Protein Structure, Tertiary</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Vero Cells</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Cellules Vero</term>
<term>Données de séquences moléculaires</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires</term>
<term>Humains</term>
<term>Lignée cellulaire</term>
<term>Protéines de l'enveloppe virale</term>
<term>Structure quaternaire des protéines</term>
<term>Structure tertiaire des protéines</term>
<term>Séquence d'acides aminés</term>
<term>Séquence nucléotidique</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The spike protein (S) of severe acute respiratory syndrome coronavirus (SARS-CoV) is responsible for receptor binding and membrane fusion. It contains a highly conserved transmembrane domain that consists of three parts: an N-terminal tryptophan-rich domain, a central domain, and a cysteine-rich C-terminal domain. The cytoplasmic tail of S has previously been shown to be required for assembly. Here, the roles of the transmembrane and cytoplasmic domains of S in the infectivity and membrane fusion activity of SARS-CoV have been studied. SARS-CoV S-pseudotyped retrovirus (SARSpp) was used to measure S-mediated infectivity. In addition, the cell-cell fusion activity of S was monitored by a Renilla luciferase-based cell-cell fusion assay. S(VSV-Cyt), an S chimera with a cytoplasmic tail derived from vesicular stomatitis virus G protein (VSV-G), and S(MHV-TMDCyt), an S chimera with the cytoplasmic and transmembrane domains of mouse hepatitis virus, displayed wild-type-like activity in both assays. S(VSV-TMDCyt), a chimera with the cytoplasmic and transmembrane domains of VSV-G, was impaired in the SARSpp and cell-cell fusion assays, showing 3 to 25% activity compared to the wild type, depending on the assay and the cells used. Examination of the oligomeric state of the chimeric S proteins in SARSpp revealed that S(VSV-TMDCyt) trimers were less stable than wild-type S trimers, possibly explaining the lowered fusogenicity and infectivity.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16415007</PMID>
<DateCompleted><Year>2006</Year>
<Month>02</Month>
<Day>27</Day>
</DateCompleted>
<DateRevised><Year>2019</Year>
<Month>12</Month>
<Day>10</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">0022-538X</ISSN>
<JournalIssue CitedMedium="Print"><Volume>80</Volume>
<Issue>3</Issue>
<PubDate><Year>2006</Year>
<Month>Feb</Month>
</PubDate>
</JournalIssue>
<Title>Journal of virology</Title>
<ISOAbbreviation>J. Virol.</ISOAbbreviation>
</Journal>
<ArticleTitle>Important role for the transmembrane domain of severe acute respiratory syndrome coronavirus spike protein during entry.</ArticleTitle>
<Pagination><MedlinePgn>1302-10</MedlinePgn>
</Pagination>
<Abstract><AbstractText>The spike protein (S) of severe acute respiratory syndrome coronavirus (SARS-CoV) is responsible for receptor binding and membrane fusion. It contains a highly conserved transmembrane domain that consists of three parts: an N-terminal tryptophan-rich domain, a central domain, and a cysteine-rich C-terminal domain. The cytoplasmic tail of S has previously been shown to be required for assembly. Here, the roles of the transmembrane and cytoplasmic domains of S in the infectivity and membrane fusion activity of SARS-CoV have been studied. SARS-CoV S-pseudotyped retrovirus (SARSpp) was used to measure S-mediated infectivity. In addition, the cell-cell fusion activity of S was monitored by a Renilla luciferase-based cell-cell fusion assay. S(VSV-Cyt), an S chimera with a cytoplasmic tail derived from vesicular stomatitis virus G protein (VSV-G), and S(MHV-TMDCyt), an S chimera with the cytoplasmic and transmembrane domains of mouse hepatitis virus, displayed wild-type-like activity in both assays. S(VSV-TMDCyt), a chimera with the cytoplasmic and transmembrane domains of VSV-G, was impaired in the SARSpp and cell-cell fusion assays, showing 3 to 25% activity compared to the wild type, depending on the assay and the cells used. Examination of the oligomeric state of the chimeric S proteins in SARSpp revealed that S(VSV-TMDCyt) trimers were less stable than wild-type S trimers, possibly explaining the lowered fusogenicity and infectivity.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Broer</LastName>
<ForeName>Rene</ForeName>
<Initials>R</Initials>
<AffiliationInfo><Affiliation>Department of Medical Microbiology, E4-P, Leiden University Medical Center, P.O. Box 9600, 2300 RC Leiden, The Netherlands.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Boson</LastName>
<ForeName>Bertrand</ForeName>
<Initials>B</Initials>
</Author>
<Author ValidYN="Y"><LastName>Spaan</LastName>
<ForeName>Willy</ForeName>
<Initials>W</Initials>
</Author>
<Author ValidYN="Y"><LastName>Cosset</LastName>
<ForeName>François-Loïc</ForeName>
<Initials>FL</Initials>
</Author>
<Author ValidYN="Y"><LastName>Corver</LastName>
<ForeName>Jeroen</ForeName>
<Initials>J</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>J Virol</MedlineTA>
<NlmUniqueID>0113724</NlmUniqueID>
<ISSNLinking>0022-538X</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D004279">DNA, Viral</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D008562">Membrane Glycoproteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D011991">Receptors, Virus</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D011993">Recombinant Fusion Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D064370">Spike Glycoprotein, Coronavirus</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D014759">Viral Envelope Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>EC 1.13.12.5</RegistryNumber>
<NameOfSubstance UI="D049410">Luciferases, Renilla</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000595" MajorTopicYN="N">Amino Acid Sequence</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002460" MajorTopicYN="N">Cell Line</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002522" MajorTopicYN="N">Chlorocebus aethiops</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D004279" MajorTopicYN="N">DNA, Viral</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D049410" MajorTopicYN="N">Luciferases, Renilla</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008561" MajorTopicYN="N">Membrane Fusion</DescriptorName>
<QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008562" MajorTopicYN="N">Membrane Glycoproteins</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008969" MajorTopicYN="N">Molecular Sequence Data</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D020836" MajorTopicYN="N">Protein Structure, Quaternary</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017434" MajorTopicYN="N">Protein Structure, Tertiary</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011991" MajorTopicYN="N">Receptors, Virus</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011993" MajorTopicYN="N">Recombinant Fusion Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000502" MajorTopicYN="N">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000472" MajorTopicYN="N">pathogenicity</QualifierName>
<QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D064370" MajorTopicYN="N">Spike Glycoprotein, Coronavirus</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014709" MajorTopicYN="N">Vero Cells</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014759" MajorTopicYN="N">Viral Envelope Proteins</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName>
<QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName>
<QualifierName UI="Q000502" MajorTopicYN="Y">physiology</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2006</Year>
<Month>1</Month>
<Day>18</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2006</Year>
<Month>2</Month>
<Day>28</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2006</Year>
<Month>1</Month>
<Day>18</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">16415007</ArticleId>
<ArticleId IdType="pii">80/3/1302</ArticleId>
<ArticleId IdType="doi">10.1128/JVI.80.3.1302-1310.2006</ArticleId>
<ArticleId IdType="pmc">PMC1346921</ArticleId>
</ArticleIdList>
<ReferenceList><Reference><Citation>Virology. 1995 Dec 20;214(2):453-63</Citation>
<ArticleIdList><ArticleId IdType="pubmed">8553547</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Gene. 1991 Dec 15;108(2):201-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">1660838</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 1999 Jul;73(7):6089-92</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10364363</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 1999 Oct;73(10):8152-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10482565</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2004 Nov 2;101(44):15748-53</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15496474</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 2004 Nov 19;279(47):49414-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15345712</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2004 Dec 28;101(52):17958-63</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15604146</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2005 Feb;79(3):1743-52</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15650199</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochemistry. 2005 Jan 25;44(3):947-58</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15654751</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2005 Jun;79(11):7195-206</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15890958</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>EMBO J. 2005 Apr 20;24(8):1634-43</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15791205</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2005 Aug 16;102(33):11876-81</Citation>
<ArticleIdList><ArticleId IdType="pubmed">16081529</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Virology. 2000 Mar 30;269(1):212-24</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10725213</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2000 Sep;74(17):8038-47</Citation>
<ArticleIdList><ArticleId IdType="pubmed">10933713</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Gene Ther. 2000 Oct;7(19):1613-23</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11083469</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Virology. 2001 Jan 20;279(2):371-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11162792</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biosci Rep. 2000 Dec;20(6):519-33</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11426692</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2002 May;76(9):4603-11</Citation>
<ArticleIdList><ArticleId IdType="pubmed">11932425</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2003 Jan;77(2):830-40</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12502799</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Exp Med. 2003 Mar 3;197(5):633-42</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12615904</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nat Rev Mol Cell Biol. 2003 Apr;4(4):309-19</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12671653</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Lancet. 2003 Apr 19;361(9366):1319-25</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12711465</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>N Engl J Med. 2003 May 15;348(20):1967-76</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12690091</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>N Engl J Med. 2003 May 15;348(20):1953-66</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12690092</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Science. 2003 May 30;300(5624):1399-404</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12730501</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2003 Aug;77(16):8801-11</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12885899</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Mol Biol. 2003 Aug 29;331(5):991-1004</Citation>
<ArticleIdList><ArticleId IdType="pubmed">12927536</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochem Biophys Res Commun. 2003 Dec 26;312(4):1159-64</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14651994</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biophys J. 2003 Dec;85(6):3769-80</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14645067</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 2003 Nov 27;426(6965):450-4</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14647384</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 2004 Jan 30;279(5):3197-201</Citation>
<ArticleIdList><ArticleId IdType="pubmed">14670965</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 2004 Mar 23;101(12):4240-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15010527</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2004 May;78(9):4552-60</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15078936</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Pathol. 2004 Jun;203(2):631-7</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15141377</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2004 Jun;78(11):5642-50</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15140961</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2004 Jun;78(12):6134-42</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15163706</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2004 Jul;78(13):7217-26</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15194798</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Biol Chem. 2004 Jul 16;279(29):30514-22</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15123674</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2004 Sep;78(17):9007-15</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15308697</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochem Biophys Res Commun. 2004 Sep 10;322(1):93-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15313178</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2004 Sep;78(18):9904-17</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15331724</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Biochem Biophys Res Commun. 2004 Sep 3;321(4):994-1000</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15358126</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2004 Oct;78(19):10328-35</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15367599</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2004 Oct;78(19):10628-35</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15367630</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 2004 Nov;78(21):12090-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">15479853</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Nature. 1970 Aug 15;227(5259):680-5</Citation>
<ArticleIdList><ArticleId IdType="pubmed">5432063</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>Proc Natl Acad Sci U S A. 1984 Apr;81(7):2035-9</Citation>
<ArticleIdList><ArticleId IdType="pubmed">6371808</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 1985 Dec;56(3):904-11</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2999443</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 1985 Dec;56(3):912-20</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2999444</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Mol Biol. 1987 Aug 20;196(4):963-6</Citation>
<ArticleIdList><ArticleId IdType="pubmed">3681988</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 1990 Nov;64(11):5367-75</Citation>
<ArticleIdList><ArticleId IdType="pubmed">2170676</ArticleId>
</ArticleIdList>
</Reference>
<Reference><Citation>J Virol. 1999 Mar;73(3):2469-80</Citation>
<ArticleIdList><ArticleId IdType="pubmed">9971832</ArticleId>
</ArticleIdList>
</Reference>
</ReferenceList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>Pays-Bas</li>
</country>
</list>
<tree><noCountry><name sortKey="Boson, Bertrand" sort="Boson, Bertrand" uniqKey="Boson B" first="Bertrand" last="Boson">Bertrand Boson</name>
<name sortKey="Corver, Jeroen" sort="Corver, Jeroen" uniqKey="Corver J" first="Jeroen" last="Corver">Jeroen Corver</name>
<name sortKey="Cosset, Francois Loic" sort="Cosset, Francois Loic" uniqKey="Cosset F" first="François-Loïc" last="Cosset">François-Loïc Cosset</name>
<name sortKey="Spaan, Willy" sort="Spaan, Willy" uniqKey="Spaan W" first="Willy" last="Spaan">Willy Spaan</name>
</noCountry>
<country name="Pays-Bas"><noRegion><name sortKey="Broer, Rene" sort="Broer, Rene" uniqKey="Broer R" first="Rene" last="Broer">Rene Broer</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Ncbi/Merge

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001337 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd -nk 001337 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    SrasV1
   |flux=    Ncbi
   |étape=   Merge
   |type=    RBID
   |clé=     pubmed:16415007
   |texte=   Important role for the transmembrane domain of severe acute respiratory syndrome coronavirus spike protein during entry.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/RBID.i   -Sk "pubmed:16415007" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd   \
       | NlmPubMed2Wicri -a SrasV1

This area was generated with Dilib version V0.6.33.
Data generation: Tue Apr 28 14:49:16 2020. Site generation: Sat Mar 27 22:06:49 2021

	Serveur d'exploration SRAS
	Attention, ce site est en cours de développement ! Attention, site généré par des moyens informatiques à partir de corpus bruts. Les informations ne sont donc pas validées.

Serveur d'exploration SRAS

Important role for the transmembrane domain of severe acute respiratory syndrome coronavirus spike protein during entry.

Important role for the transmembrane domain of severe acute respiratory syndrome coronavirus spike protein during entry.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki