[Expression of SARS coronavirus nucleocapsid protein and construction of its DNA vaccine].
Identifieur interne : 001236 ( Ncbi/Merge ); précédent : 001235; suivant : 001237[Expression of SARS coronavirus nucleocapsid protein and construction of its DNA vaccine].
Auteurs : Kai-Jun Xiang [République populaire de Chine] ; Qi-Yi Zeng ; Yong-Qiang Ding ; Bing Zhu ; Rong ZhouSource :
- Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology [ 1007-8738 ] ; 2005.
Descripteurs français
- KwdFr :
- Protéines nucléocapside (génétique), Protéines nucléocapside (immunologie), Protéines nucléocapside (isolement et purification), Protéines nucléocapside (métabolisme), Test ELISA, Vaccins à ADN (génétique), Vaccins à ADN (immunologie), Virus du SRAS (génétique), Virus du SRAS (immunologie), Électrophorèse sur gel de polyacrylamide.
- MESH :
- génétique : Protéines nucléocapside, Vaccins à ADN, Virus du SRAS.
- immunologie : Protéines nucléocapside, Vaccins à ADN, Virus du SRAS.
- isolement et purification : Protéines nucléocapside.
- métabolisme : Protéines nucléocapside.
- Test ELISA, Électrophorèse sur gel de polyacrylamide.
English descriptors
- KwdEn :
- Electrophoresis, Polyacrylamide Gel, Enzyme-Linked Immunosorbent Assay, Nucleocapsid Proteins (genetics), Nucleocapsid Proteins (immunology), Nucleocapsid Proteins (isolation & purification), Nucleocapsid Proteins (metabolism), SARS Virus (genetics), SARS Virus (immunology), Vaccines, DNA (genetics), Vaccines, DNA (immunology).
- MESH :
- chemical , genetics : Nucleocapsid Proteins, Vaccines, DNA.
- chemical , immunology : Nucleocapsid Proteins, Vaccines, DNA.
- chemical , isolation & purification : Nucleocapsid Proteins.
- chemical , metabolism : Nucleocapsid Proteins.
- genetics : SARS Virus.
- immunology : SARS Virus.
- Electrophoresis, Polyacrylamide Gel, Enzyme-Linked Immunosorbent Assay.
Abstract
To express the nucleocapsid (N) protein of SARS coronavirus (SARS-CoV) in E. coli and construct its DNA vaccine.
PubMed: 16256037
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pubmed:16256037Le document en format XML
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<author><name sortKey="Xiang, Kai Jun" sort="Xiang, Kai Jun" uniqKey="Xiang K" first="Kai-Jun" last="Xiang">Kai-Jun Xiang</name>
<affiliation wicri:level="1"><nlm:affiliation>Guangzhou Huayin Genetic Science and Technology Co., Ltd., Guangzhou 510150, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Guangzhou Huayin Genetic Science and Technology Co., Ltd., Guangzhou 510150</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Zeng, Qi Yi" sort="Zeng, Qi Yi" uniqKey="Zeng Q" first="Qi-Yi" last="Zeng">Qi-Yi Zeng</name>
</author>
<author><name sortKey="Ding, Yong Qiang" sort="Ding, Yong Qiang" uniqKey="Ding Y" first="Yong-Qiang" last="Ding">Yong-Qiang Ding</name>
</author>
<author><name sortKey="Zhu, Bing" sort="Zhu, Bing" uniqKey="Zhu B" first="Bing" last="Zhu">Bing Zhu</name>
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<author><name sortKey="Zhou, Rong" sort="Zhou, Rong" uniqKey="Zhou R" first="Rong" last="Zhou">Rong Zhou</name>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">[Expression of SARS coronavirus nucleocapsid protein and construction of its DNA vaccine].</title>
<author><name sortKey="Xiang, Kai Jun" sort="Xiang, Kai Jun" uniqKey="Xiang K" first="Kai-Jun" last="Xiang">Kai-Jun Xiang</name>
<affiliation wicri:level="1"><nlm:affiliation>Guangzhou Huayin Genetic Science and Technology Co., Ltd., Guangzhou 510150, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Guangzhou Huayin Genetic Science and Technology Co., Ltd., Guangzhou 510150</wicri:regionArea>
<placeName><settlement type="city">Jiangmen</settlement>
<region type="province">Guangdong</region>
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<author><name sortKey="Zeng, Qi Yi" sort="Zeng, Qi Yi" uniqKey="Zeng Q" first="Qi-Yi" last="Zeng">Qi-Yi Zeng</name>
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<author><name sortKey="Ding, Yong Qiang" sort="Ding, Yong Qiang" uniqKey="Ding Y" first="Yong-Qiang" last="Ding">Yong-Qiang Ding</name>
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<author><name sortKey="Zhu, Bing" sort="Zhu, Bing" uniqKey="Zhu B" first="Bing" last="Zhu">Bing Zhu</name>
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<author><name sortKey="Zhou, Rong" sort="Zhou, Rong" uniqKey="Zhou R" first="Rong" last="Zhou">Rong Zhou</name>
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<series><title level="j">Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology</title>
<idno type="ISSN">1007-8738</idno>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Electrophoresis, Polyacrylamide Gel</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Nucleocapsid Proteins (genetics)</term>
<term>Nucleocapsid Proteins (immunology)</term>
<term>Nucleocapsid Proteins (isolation & purification)</term>
<term>Nucleocapsid Proteins (metabolism)</term>
<term>SARS Virus (genetics)</term>
<term>SARS Virus (immunology)</term>
<term>Vaccines, DNA (genetics)</term>
<term>Vaccines, DNA (immunology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Protéines nucléocapside (génétique)</term>
<term>Protéines nucléocapside (immunologie)</term>
<term>Protéines nucléocapside (isolement et purification)</term>
<term>Protéines nucléocapside (métabolisme)</term>
<term>Test ELISA</term>
<term>Vaccins à ADN (génétique)</term>
<term>Vaccins à ADN (immunologie)</term>
<term>Virus du SRAS (génétique)</term>
<term>Virus du SRAS (immunologie)</term>
<term>Électrophorèse sur gel de polyacrylamide</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Nucleocapsid Proteins</term>
<term>Vaccines, DNA</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Nucleocapsid Proteins</term>
<term>Vaccines, DNA</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Nucleocapsid Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Nucleocapsid Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines nucléocapside</term>
<term>Vaccins à ADN</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Protéines nucléocapside</term>
<term>Vaccins à ADN</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Protéines nucléocapside</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines nucléocapside</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Electrophoresis, Polyacrylamide Gel</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Test ELISA</term>
<term>Électrophorèse sur gel de polyacrylamide</term>
</keywords>
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<front><div type="abstract" xml:lang="en">To express the nucleocapsid (N) protein of SARS coronavirus (SARS-CoV) in E. coli and construct its DNA vaccine.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16256037</PMID>
<DateCompleted><Year>2009</Year>
<Month>04</Month>
<Day>23</Day>
</DateCompleted>
<DateRevised><Year>2005</Year>
<Month>10</Month>
<Day>31</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">1007-8738</ISSN>
<JournalIssue CitedMedium="Print"><Volume>21</Volume>
<Issue>6</Issue>
<PubDate><Year>2005</Year>
<Month>Nov</Month>
</PubDate>
</JournalIssue>
<Title>Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology</Title>
<ISOAbbreviation>Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi</ISOAbbreviation>
</Journal>
<ArticleTitle>[Expression of SARS coronavirus nucleocapsid protein and construction of its DNA vaccine].</ArticleTitle>
<Pagination><MedlinePgn>731-3, 737</MedlinePgn>
</Pagination>
<Abstract><AbstractText Label="AIM" NlmCategory="OBJECTIVE">To express the nucleocapsid (N) protein of SARS coronavirus (SARS-CoV) in E. coli and construct its DNA vaccine.</AbstractText>
<AbstractText Label="METHODS" NlmCategory="METHODS">The prokaryotic expression vector pQEN containing N gene was constructed and transformed into the E. coli. The recombinant N protein was then expressed and purified by Ni(2+)-NTA affinity resin. In addition, the N gene was cloned into the eukaryotic expression plasmid pSecTagB and the eukaryotic recombinant expression vector pSecN was obtained. The DNA vaccine pSecN was injected to immunize the BALB/c mice to produce the antiserum against N protein of SARS-CoV. Subsequently, the reactivity of the antiserum with recombinant N protein and SARS-CoV particles was assayed by ELISA.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">Recombinant N protein reacted strongly and specifically with the sera from immunized mice and SARS patients. Similarly, the sera of immunized mice could also react specifically with SARS-CoV particles.</AbstractText>
<AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">The recombinant N protein could be used as a good antigen to detect SARS. The DNA vaccine pSecN could also efficiently induce the production of IgG against N protein of SARS-CoV, which offered clues to the development of a potential DNA vaccine.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Xiang</LastName>
<ForeName>Kai-jun</ForeName>
<Initials>KJ</Initials>
<AffiliationInfo><Affiliation>Guangzhou Huayin Genetic Science and Technology Co., Ltd., Guangzhou 510150, China.</Affiliation>
</AffiliationInfo>
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<Author ValidYN="Y"><LastName>Zeng</LastName>
<ForeName>Qi-yi</ForeName>
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<Author ValidYN="Y"><LastName>Ding</LastName>
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<Author ValidYN="Y"><LastName>Zhu</LastName>
<ForeName>Bing</ForeName>
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<Author ValidYN="Y"><LastName>Zhou</LastName>
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<Language>chi</Language>
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<MedlineJournalInfo><Country>China</Country>
<MedlineTA>Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi</MedlineTA>
<NlmUniqueID>101139110</NlmUniqueID>
<ISSNLinking>1007-8738</ISSNLinking>
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<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D019590">Nucleocapsid Proteins</NameOfSubstance>
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<NameOfSubstance UI="D019444">Vaccines, DNA</NameOfSubstance>
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<NameOfSubstance UI="C099602">nucleocapsid protein, Coronavirus</NameOfSubstance>
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<MeshHeadingList><MeshHeading><DescriptorName UI="D004591" MajorTopicYN="N">Electrophoresis, Polyacrylamide Gel</DescriptorName>
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<MeshHeading><DescriptorName UI="D004797" MajorTopicYN="N">Enzyme-Linked Immunosorbent Assay</DescriptorName>
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<MeshHeading><DescriptorName UI="D019590" MajorTopicYN="N">Nucleocapsid Proteins</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
<QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName>
<QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D019444" MajorTopicYN="N">Vaccines, DNA</DescriptorName>
<QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName>
<QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName>
</MeshHeading>
</MeshHeadingList>
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<settlement><li>Jiangmen</li>
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<tree><noCountry><name sortKey="Ding, Yong Qiang" sort="Ding, Yong Qiang" uniqKey="Ding Y" first="Yong-Qiang" last="Ding">Yong-Qiang Ding</name>
<name sortKey="Zeng, Qi Yi" sort="Zeng, Qi Yi" uniqKey="Zeng Q" first="Qi-Yi" last="Zeng">Qi-Yi Zeng</name>
<name sortKey="Zhou, Rong" sort="Zhou, Rong" uniqKey="Zhou R" first="Rong" last="Zhou">Rong Zhou</name>
<name sortKey="Zhu, Bing" sort="Zhu, Bing" uniqKey="Zhu B" first="Bing" last="Zhu">Bing Zhu</name>
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