[Expression of SARS coronavirus nucleocapsid protein and construction of its DNA vaccine].
Identifieur interne : 001236 ( Ncbi/Merge ); précédent : 001235; suivant : 001237[Expression of SARS coronavirus nucleocapsid protein and construction of its DNA vaccine].
Auteurs : Kai-Jun Xiang [République populaire de Chine] ; Qi-Yi Zeng ; Yong-Qiang Ding ; Bing Zhu ; Rong ZhouSource :
- Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology [ 1007-8738 ] ; 2005.
Descripteurs français
- KwdFr :
- Protéines nucléocapside (génétique), Protéines nucléocapside (immunologie), Protéines nucléocapside (isolement et purification), Protéines nucléocapside (métabolisme), Test ELISA, Vaccins à ADN (génétique), Vaccins à ADN (immunologie), Virus du SRAS (génétique), Virus du SRAS (immunologie), Électrophorèse sur gel de polyacrylamide.
- MESH :
- génétique : Protéines nucléocapside, Vaccins à ADN, Virus du SRAS.
- immunologie : Protéines nucléocapside, Vaccins à ADN, Virus du SRAS.
- isolement et purification : Protéines nucléocapside.
- métabolisme : Protéines nucléocapside.
- Test ELISA, Électrophorèse sur gel de polyacrylamide.
English descriptors
- KwdEn :
- Electrophoresis, Polyacrylamide Gel, Enzyme-Linked Immunosorbent Assay, Nucleocapsid Proteins (genetics), Nucleocapsid Proteins (immunology), Nucleocapsid Proteins (isolation & purification), Nucleocapsid Proteins (metabolism), SARS Virus (genetics), SARS Virus (immunology), Vaccines, DNA (genetics), Vaccines, DNA (immunology).
- MESH :
- chemical , genetics : Nucleocapsid Proteins, Vaccines, DNA.
- chemical , immunology : Nucleocapsid Proteins, Vaccines, DNA.
- chemical , isolation & purification : Nucleocapsid Proteins.
- chemical , metabolism : Nucleocapsid Proteins.
- genetics : SARS Virus.
- immunology : SARS Virus.
- Electrophoresis, Polyacrylamide Gel, Enzyme-Linked Immunosorbent Assay.
Abstract
To express the nucleocapsid (N) protein of SARS coronavirus (SARS-CoV) in E. coli and construct its DNA vaccine.
PubMed: 16256037
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pubmed:16256037Le document en format XML
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Ding</name></author><author><name sortKey="Zhu, Bing" sort="Zhu, Bing" uniqKey="Zhu B" first="Bing" last="Zhu">Bing Zhu</name></author><author><name sortKey="Zhou, Rong" sort="Zhou, Rong" uniqKey="Zhou R" first="Rong" last="Zhou">Rong Zhou</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:16256037</idno><idno type="pmid">16256037</idno><idno type="wicri:Area/PubMed/Corpus">002472</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002472</idno><idno type="wicri:Area/PubMed/Curation">002472</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002472</idno><idno type="wicri:Area/PubMed/Checkpoint">002367</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002367</idno><idno type="wicri:Area/Ncbi/Merge">001236</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">[Expression of SARS coronavirus nucleocapsid protein and construction of its DNA vaccine].</title><author><name sortKey="Xiang, Kai Jun" sort="Xiang, Kai Jun" uniqKey="Xiang K" first="Kai-Jun" last="Xiang">Kai-Jun Xiang</name><affiliation wicri:level="1"><nlm:affiliation>Guangzhou Huayin Genetic Science and Technology Co., Ltd., Guangzhou 510150, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangzhou Huayin Genetic Science and Technology Co., Ltd., Guangzhou 510150</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Zeng, Qi Yi" sort="Zeng, Qi Yi" uniqKey="Zeng Q" first="Qi-Yi" last="Zeng">Qi-Yi Zeng</name></author><author><name sortKey="Ding, Yong Qiang" sort="Ding, Yong Qiang" uniqKey="Ding Y" first="Yong-Qiang" last="Ding">Yong-Qiang Ding</name></author><author><name sortKey="Zhu, Bing" sort="Zhu, Bing" uniqKey="Zhu B" first="Bing" last="Zhu">Bing Zhu</name></author><author><name sortKey="Zhou, Rong" sort="Zhou, Rong" uniqKey="Zhou R" first="Rong" last="Zhou">Rong Zhou</name></author></analytic><series><title level="j">Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology</title><idno type="ISSN">1007-8738</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Electrophoresis, Polyacrylamide Gel</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Nucleocapsid Proteins (genetics)</term><term>Nucleocapsid Proteins (immunology)</term><term>Nucleocapsid Proteins (isolation & purification)</term><term>Nucleocapsid Proteins (metabolism)</term><term>SARS Virus (genetics)</term><term>SARS Virus (immunology)</term><term>Vaccines, DNA (genetics)</term><term>Vaccines, DNA (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Protéines nucléocapside (génétique)</term><term>Protéines nucléocapside (immunologie)</term><term>Protéines nucléocapside (isolement et purification)</term><term>Protéines nucléocapside (métabolisme)</term><term>Test ELISA</term><term>Vaccins à ADN (génétique)</term><term>Vaccins à ADN (immunologie)</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (immunologie)</term><term>Électrophorèse sur gel de polyacrylamide</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Nucleocapsid Proteins</term><term>Vaccines, DNA</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Nucleocapsid Proteins</term><term>Vaccines, DNA</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Nucleocapsid Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Nucleocapsid Proteins</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines nucléocapside</term><term>Vaccins à ADN</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Protéines nucléocapside</term><term>Vaccins à ADN</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Protéines nucléocapside</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines nucléocapside</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Electrophoresis, Polyacrylamide Gel</term><term>Enzyme-Linked Immunosorbent Assay</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Test ELISA</term><term>Électrophorèse sur gel de polyacrylamide</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To express the nucleocapsid (N) protein of SARS coronavirus (SARS-CoV) in E. coli and construct its DNA vaccine.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16256037</PMID><DateCompleted><Year>2009</Year><Month>04</Month><Day>23</Day></DateCompleted><DateRevised><Year>2005</Year><Month>10</Month><Day>31</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1007-8738</ISSN><JournalIssue CitedMedium="Print"><Volume>21</Volume><Issue>6</Issue><PubDate><Year>2005</Year><Month>Nov</Month></PubDate></JournalIssue><Title>Xi bao yu fen zi mian yi xue za zhi = Chinese journal of cellular and molecular immunology</Title><ISOAbbreviation>Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi</ISOAbbreviation></Journal><ArticleTitle>[Expression of SARS coronavirus nucleocapsid protein and construction of its DNA vaccine].</ArticleTitle><Pagination><MedlinePgn>731-3, 737</MedlinePgn></Pagination><Abstract><AbstractText Label="AIM" NlmCategory="OBJECTIVE">To express the nucleocapsid (N) protein of SARS coronavirus (SARS-CoV) in E. coli and construct its DNA vaccine.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">The prokaryotic expression vector pQEN containing N gene was constructed and transformed into the E. coli. The recombinant N protein was then expressed and purified by Ni(2+)-NTA affinity resin. In addition, the N gene was cloned into the eukaryotic expression plasmid pSecTagB and the eukaryotic recombinant expression vector pSecN was obtained. The DNA vaccine pSecN was injected to immunize the BALB/c mice to produce the antiserum against N protein of SARS-CoV. Subsequently, the reactivity of the antiserum with recombinant N protein and SARS-CoV particles was assayed by ELISA.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">Recombinant N protein reacted strongly and specifically with the sera from immunized mice and SARS patients. Similarly, the sera of immunized mice could also react specifically with SARS-CoV particles.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">The recombinant N protein could be used as a good antigen to detect SARS. The DNA vaccine pSecN could also efficiently induce the production of IgG against N protein of SARS-CoV, which offered clues to the development of a potential DNA vaccine.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Xiang</LastName><ForeName>Kai-jun</ForeName><Initials>KJ</Initials><AffiliationInfo><Affiliation>Guangzhou Huayin Genetic Science and Technology Co., Ltd., Guangzhou 510150, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zeng</LastName><ForeName>Qi-yi</ForeName><Initials>QY</Initials></Author><Author ValidYN="Y"><LastName>Ding</LastName><ForeName>Yong-qiang</ForeName><Initials>YQ</Initials></Author><Author ValidYN="Y"><LastName>Zhu</LastName><ForeName>Bing</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Zhou</LastName><ForeName>Rong</ForeName><Initials>R</Initials></Author></AuthorList><Language>chi</Language><PublicationTypeList><PublicationType UI="D004740">English Abstract</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Xi Bao Yu Fen Zi Mian Yi Xue Za Zhi</MedlineTA><NlmUniqueID>101139110</NlmUniqueID><ISSNLinking>1007-8738</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019590">Nucleocapsid Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019444">Vaccines, DNA</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C099602">nucleocapsid protein, Coronavirus</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D004591" MajorTopicYN="N">Electrophoresis, Polyacrylamide Gel</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004797" MajorTopicYN="N">Enzyme-Linked Immunosorbent Assay</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019590" MajorTopicYN="N">Nucleocapsid Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019444" MajorTopicYN="N">Vaccines, DNA</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2005</Year><Month>11</Month><Day>1</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>4</Month><Day>25</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2005</Year><Month>11</Month><Day>1</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16256037</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Guangdong</li></region><settlement><li>Jiangmen</li></settlement></list><tree><noCountry><name sortKey="Ding, Yong Qiang" sort="Ding, Yong Qiang" uniqKey="Ding Y" first="Yong-Qiang" last="Ding">Yong-Qiang Ding</name><name sortKey="Zeng, Qi Yi" sort="Zeng, Qi Yi" uniqKey="Zeng Q" first="Qi-Yi" last="Zeng">Qi-Yi Zeng</name><name sortKey="Zhou, Rong" sort="Zhou, Rong" uniqKey="Zhou R" first="Rong" last="Zhou">Rong Zhou</name><name sortKey="Zhu, Bing" sort="Zhu, Bing" uniqKey="Zhu B" first="Bing" last="Zhu">Bing Zhu</name></noCountry><country name="République populaire de Chine"><region name="Guangdong"><name sortKey="Xiang, Kai Jun" sort="Xiang, Kai Jun" uniqKey="Xiang K" first="Kai-Jun" last="Xiang">Kai-Jun Xiang</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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