SrasV1, Ncbi, Merge, bibRecord, 001211

[Detection of SARS-coronavirus in both human and animals by RT-PCR].

Identifieur interne : 001211 ( Ncbi/Merge ); précédent : 001210; suivant : 001212

[Detection of SARS-coronavirus in both human and animals by RT-PCR].

Auteurs : Jin Zhao [République populaire de Chine] ; Shi-Song Fang ; Ya-Qing He ; Hong Yang ; Jianjun Liu ; Qinghua Hu ; Jianfan He ; Tao Liu ; Xiaoli Liu ; Zhixiong Zhuang ; Dan Zhang ; Jun'An Zhou

Source :

Wei sheng yan jiu = Journal of hygiene research [ 1000-8020 ] ; 2005.

RBID : pubmed:16229261

Descripteurs français

KwdFr :
- Animaux, Chine, Humains, Nez (virologie), Pharynx (virologie), RT-PCR (), Virus du SRAS (isolement et purification), Viverridae (virologie).
MESH :
- isolement et purification : Virus du SRAS.
- virologie : Nez, Pharynx, Viverridae.
- Animaux, Chine, Humains, RT-PCR.
Wicri :
- geographic : République populaire de Chine.

English descriptors

KwdEn :
- Animals, China, Humans, Nose (virology), Pharynx (virology), Reverse Transcriptase Polymerase Chain Reaction (methods), SARS Virus (isolation & purification), Viverridae (virology).
MESH :
- geographic : China.
- isolation & purification : SARS Virus.
- methods : Reverse Transcriptase Polymerase Chain Reaction.
- virology : Nose, Pharynx, Viverridae.
- Animals, Humans.

Abstract

To find the most suitable RT-PCR detection method for SARS-Coronavirus (SARS-CoV) detection in both human and animals, and to study the source of SARS virus by investigating the condition of virus carried by wild, domestic animals and animals sold in market.

PubMed: 16229261

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to stream PubMed, to step Checkpoint: 002379

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pubmed:16229261

Le document en format XML

<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">[Detection of SARS-coronavirus in both human and animals by RT-PCR].</title>
<author><name sortKey="Zhao, Jin" sort="Zhao, Jin" uniqKey="Zhao J" first="Jin" last="Zhao">Jin Zhao</name>
<affiliation wicri:level="1"><nlm:affiliation>Shenzhen Center for Disease Control and Prevention, Shenzhen 518020, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Shenzhen Center for Disease Control and Prevention, Shenzhen 518020</wicri:regionArea>
<placeName><settlement type="city">Shenzhen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Fang, Shi Song" sort="Fang, Shi Song" uniqKey="Fang S" first="Shi-Song" last="Fang">Shi-Song Fang</name>
</author>
<author><name sortKey="He, Ya Qing" sort="He, Ya Qing" uniqKey="He Y" first="Ya-Qing" last="He">Ya-Qing He</name>
</author>
<author><name sortKey="Yang, Hong" sort="Yang, Hong" uniqKey="Yang H" first="Hong" last="Yang">Hong Yang</name>
</author>
<author><name sortKey="Liu, Jianjun" sort="Liu, Jianjun" uniqKey="Liu J" first="Jianjun" last="Liu">Jianjun Liu</name>
</author>
<author><name sortKey="Hu, Qinghua" sort="Hu, Qinghua" uniqKey="Hu Q" first="Qinghua" last="Hu">Qinghua Hu</name>
</author>
<author><name sortKey="He, Jianfan" sort="He, Jianfan" uniqKey="He J" first="Jianfan" last="He">Jianfan He</name>
</author>
<author><name sortKey="Liu, Tao" sort="Liu, Tao" uniqKey="Liu T" first="Tao" last="Liu">Tao Liu</name>
</author>
<author><name sortKey="Liu, Xiaoli" sort="Liu, Xiaoli" uniqKey="Liu X" first="Xiaoli" last="Liu">Xiaoli Liu</name>
</author>
<author><name sortKey="Zhuang, Zhixiong" sort="Zhuang, Zhixiong" uniqKey="Zhuang Z" first="Zhixiong" last="Zhuang">Zhixiong Zhuang</name>
</author>
<author><name sortKey="Zhang, Dan" sort="Zhang, Dan" uniqKey="Zhang D" first="Dan" last="Zhang">Dan Zhang</name>
</author>
<author><name sortKey="Zhou, Jun An" sort="Zhou, Jun An" uniqKey="Zhou J" first="Jun'An" last="Zhou">Jun'An Zhou</name>
</author>
</titleStmt>
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<date when="2005">2005</date>
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<sourceDesc><biblStruct><analytic><title xml:lang="en">[Detection of SARS-coronavirus in both human and animals by RT-PCR].</title>
<author><name sortKey="Zhao, Jin" sort="Zhao, Jin" uniqKey="Zhao J" first="Jin" last="Zhao">Jin Zhao</name>
<affiliation wicri:level="1"><nlm:affiliation>Shenzhen Center for Disease Control and Prevention, Shenzhen 518020, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Shenzhen Center for Disease Control and Prevention, Shenzhen 518020</wicri:regionArea>
<placeName><settlement type="city">Shenzhen</settlement>
<region type="province">Guangdong</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Fang, Shi Song" sort="Fang, Shi Song" uniqKey="Fang S" first="Shi-Song" last="Fang">Shi-Song Fang</name>
</author>
<author><name sortKey="He, Ya Qing" sort="He, Ya Qing" uniqKey="He Y" first="Ya-Qing" last="He">Ya-Qing He</name>
</author>
<author><name sortKey="Yang, Hong" sort="Yang, Hong" uniqKey="Yang H" first="Hong" last="Yang">Hong Yang</name>
</author>
<author><name sortKey="Liu, Jianjun" sort="Liu, Jianjun" uniqKey="Liu J" first="Jianjun" last="Liu">Jianjun Liu</name>
</author>
<author><name sortKey="Hu, Qinghua" sort="Hu, Qinghua" uniqKey="Hu Q" first="Qinghua" last="Hu">Qinghua Hu</name>
</author>
<author><name sortKey="He, Jianfan" sort="He, Jianfan" uniqKey="He J" first="Jianfan" last="He">Jianfan He</name>
</author>
<author><name sortKey="Liu, Tao" sort="Liu, Tao" uniqKey="Liu T" first="Tao" last="Liu">Tao Liu</name>
</author>
<author><name sortKey="Liu, Xiaoli" sort="Liu, Xiaoli" uniqKey="Liu X" first="Xiaoli" last="Liu">Xiaoli Liu</name>
</author>
<author><name sortKey="Zhuang, Zhixiong" sort="Zhuang, Zhixiong" uniqKey="Zhuang Z" first="Zhixiong" last="Zhuang">Zhixiong Zhuang</name>
</author>
<author><name sortKey="Zhang, Dan" sort="Zhang, Dan" uniqKey="Zhang D" first="Dan" last="Zhang">Dan Zhang</name>
</author>
<author><name sortKey="Zhou, Jun An" sort="Zhou, Jun An" uniqKey="Zhou J" first="Jun'An" last="Zhou">Jun'An Zhou</name>
</author>
</analytic>
<series><title level="j">Wei sheng yan jiu = Journal of hygiene research</title>
<idno type="ISSN">1000-8020</idno>
<imprint><date when="2005" type="published">2005</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>China</term>
<term>Humans</term>
<term>Nose (virology)</term>
<term>Pharynx (virology)</term>
<term>Reverse Transcriptase Polymerase Chain Reaction (methods)</term>
<term>SARS Virus (isolation & purification)</term>
<term>Viverridae (virology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Chine</term>
<term>Humains</term>
<term>Nez (virologie)</term>
<term>Pharynx (virologie)</term>
<term>RT-PCR ()</term>
<term>Virus du SRAS (isolement et purification)</term>
<term>Viverridae (virologie)</term>
</keywords>
<keywords scheme="MESH" type="geographic" xml:lang="en"><term>China</term>
</keywords>
<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Reverse Transcriptase Polymerase Chain Reaction</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Nez</term>
<term>Pharynx</term>
<term>Viverridae</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Nose</term>
<term>Pharynx</term>
<term>Viverridae</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Humans</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Chine</term>
<term>Humains</term>
<term>RT-PCR</term>
</keywords>
<keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>République populaire de Chine</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">To find the most suitable RT-PCR detection method for SARS-Coronavirus (SARS-CoV) detection in both human and animals, and to study the source of SARS virus by investigating the condition of virus carried by wild, domestic animals and animals sold in market.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16229261</PMID>
<DateCompleted><Year>2010</Year>
<Month>07</Month>
<Day>29</Day>
</DateCompleted>
<DateRevised><Year>2005</Year>
<Month>10</Month>
<Day>18</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">1000-8020</ISSN>
<JournalIssue CitedMedium="Print"><Volume>34</Volume>
<Issue>4</Issue>
<PubDate><Year>2005</Year>
<Month>Jul</Month>
</PubDate>
</JournalIssue>
<Title>Wei sheng yan jiu = Journal of hygiene research</Title>
<ISOAbbreviation>Wei Sheng Yan Jiu</ISOAbbreviation>
</Journal>
<ArticleTitle>[Detection of SARS-coronavirus in both human and animals by RT-PCR].</ArticleTitle>
<Pagination><MedlinePgn>412-5</MedlinePgn>
</Pagination>
<Abstract><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">To find the most suitable RT-PCR detection method for SARS-Coronavirus (SARS-CoV) detection in both human and animals, and to study the source of SARS virus by investigating the condition of virus carried by wild, domestic animals and animals sold in market.</AbstractText>
<AbstractText Label="METHODS" NlmCategory="METHODS">350 throat washes of confirmed, suspected and observed SARS cases were tested by TaqMan and molecular beacon fluorescence RT-PCR methods. 386 animals with 442 nasal-throat swabs, fecal swabs of animals and cell cultured were detected by TaqMan method and conventional RT-PCR method.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">10 positive were detected from 41 SARS clinical confirmed cases (24.39%). The results of TaqMan and molecular beacon are basically coincident with the coincident rate of 88.89%. 18 cell cultures with CPE were detected and find 16 positive, among which, 14 were civet cats (87.5%). The detecting results of animal samples from Dongmen Market (Shenzhen) and other markets and farms in peripheral areas show that: the total positive rate of 4 kinds of wild animal (civet cat, raccoon dog, hog-badge and Chinese ferret-badge) is 39.02% , which has an significant difference (P < 0.01) compared with the positive rate of other animals as 0. The positive rate of PCR for nasal and fecal swabs from 109 major wild animals is 44.04%, but the positive rate of 145 wild live-animals is 0.</AbstractText>
<AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">TaqMan and molecular beacon PCR methods both can be used in SARS detection.The results could support the hypothesis that animals (especially civet cat) could carry SARS virus.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhao</LastName>
<ForeName>Jin</ForeName>
<Initials>J</Initials>
<AffiliationInfo><Affiliation>Shenzhen Center for Disease Control and Prevention, Shenzhen 518020, China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Fang</LastName>
<ForeName>Shi-Song</ForeName>
<Initials>SS</Initials>
</Author>
<Author ValidYN="Y"><LastName>He</LastName>
<ForeName>Ya-Qing</ForeName>
<Initials>YQ</Initials>
</Author>
<Author ValidYN="Y"><LastName>Yang</LastName>
<ForeName>Hong</ForeName>
<Initials>H</Initials>
</Author>
<Author ValidYN="Y"><LastName>Liu</LastName>
<ForeName>Jianjun</ForeName>
<Initials>J</Initials>
</Author>
<Author ValidYN="Y"><LastName>Hu</LastName>
<ForeName>Qinghua</ForeName>
<Initials>Q</Initials>
</Author>
<Author ValidYN="Y"><LastName>He</LastName>
<ForeName>Jianfan</ForeName>
<Initials>J</Initials>
</Author>
<Author ValidYN="Y"><LastName>Liu</LastName>
<ForeName>Tao</ForeName>
<Initials>T</Initials>
</Author>
<Author ValidYN="Y"><LastName>Liu</LastName>
<ForeName>Xiaoli</ForeName>
<Initials>X</Initials>
</Author>
<Author ValidYN="Y"><LastName>Zhuang</LastName>
<ForeName>Zhixiong</ForeName>
<Initials>Z</Initials>
</Author>
<Author ValidYN="Y"><LastName>Zhang</LastName>
<ForeName>Dan</ForeName>
<Initials>D</Initials>
</Author>
<Author ValidYN="Y"><LastName>Zhou</LastName>
<ForeName>Jun'an</ForeName>
<Initials>J</Initials>
</Author>
</AuthorList>
<Language>chi</Language>
<PublicationTypeList><PublicationType UI="D004740">English Abstract</PublicationType>
<PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
</Article>
<MedlineJournalInfo><Country>China</Country>
<MedlineTA>Wei Sheng Yan Jiu</MedlineTA>
<NlmUniqueID>9426367</NlmUniqueID>
<ISSNLinking>1000-8020</ISSNLinking>
</MedlineJournalInfo>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D002681" MajorTopicYN="N" Type="Geographic">China</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D009666" MajorTopicYN="N">Nose</DescriptorName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D010614" MajorTopicYN="N">Pharynx</DescriptorName>
<QualifierName UI="Q000821" MajorTopicYN="Y">virology</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D020133" MajorTopicYN="N">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName>
<QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName>
<QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D045949" MajorTopicYN="N">Viverridae</DescriptorName>
<QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2005</Year>
<Month>10</Month>
<Day>19</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2010</Year>
<Month>7</Month>
<Day>30</Day>
<Hour>6</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2005</Year>
<Month>10</Month>
<Day>19</Day>
<Hour>9</Hour>
<Minute>0</Minute>
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</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">16229261</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>République populaire de Chine</li>
</country>
<region><li>Guangdong</li>
</region>
<settlement><li>Shenzhen</li>
</settlement>
</list>
<tree><noCountry><name sortKey="Fang, Shi Song" sort="Fang, Shi Song" uniqKey="Fang S" first="Shi-Song" last="Fang">Shi-Song Fang</name>
<name sortKey="He, Jianfan" sort="He, Jianfan" uniqKey="He J" first="Jianfan" last="He">Jianfan He</name>
<name sortKey="He, Ya Qing" sort="He, Ya Qing" uniqKey="He Y" first="Ya-Qing" last="He">Ya-Qing He</name>
<name sortKey="Hu, Qinghua" sort="Hu, Qinghua" uniqKey="Hu Q" first="Qinghua" last="Hu">Qinghua Hu</name>
<name sortKey="Liu, Jianjun" sort="Liu, Jianjun" uniqKey="Liu J" first="Jianjun" last="Liu">Jianjun Liu</name>
<name sortKey="Liu, Tao" sort="Liu, Tao" uniqKey="Liu T" first="Tao" last="Liu">Tao Liu</name>
<name sortKey="Liu, Xiaoli" sort="Liu, Xiaoli" uniqKey="Liu X" first="Xiaoli" last="Liu">Xiaoli Liu</name>
<name sortKey="Yang, Hong" sort="Yang, Hong" uniqKey="Yang H" first="Hong" last="Yang">Hong Yang</name>
<name sortKey="Zhang, Dan" sort="Zhang, Dan" uniqKey="Zhang D" first="Dan" last="Zhang">Dan Zhang</name>
<name sortKey="Zhou, Jun An" sort="Zhou, Jun An" uniqKey="Zhou J" first="Jun'An" last="Zhou">Jun'An Zhou</name>
<name sortKey="Zhuang, Zhixiong" sort="Zhuang, Zhixiong" uniqKey="Zhuang Z" first="Zhixiong" last="Zhuang">Zhixiong Zhuang</name>
</noCountry>
<country name="République populaire de Chine"><region name="Guangdong"><name sortKey="Zhao, Jin" sort="Zhao, Jin" uniqKey="Zhao J" first="Jin" last="Zhao">Jin Zhao</name>
</region>
</country>
</tree>
</affiliations>
</record>

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[Detection of SARS-coronavirus in both human and animals by RT-PCR].

[Detection of SARS-coronavirus in both human and animals by RT-PCR].

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki