[Molecular cloning and expression of the severe acute respiratory syndrome-associated coronavirus nucleocapsid protein and its clinical application].
Identifieur interne : 001187 ( Ncbi/Merge ); précédent : 001186; suivant : 001188[Molecular cloning and expression of the severe acute respiratory syndrome-associated coronavirus nucleocapsid protein and its clinical application].
Auteurs : Jian Lu [République populaire de Chine] ; Bai-Ping Zhou ; Yu-Sen Zhou ; Xiao-Ling Jiang ; Li-Xia Wen ; Xiao-Hua Le ; Bing Li ; Liu-Mei Xu ; Li-Xiong LiSource :
- Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology [ 1003-9279 ] ; 2005.
Descripteurs français
- KwdFr :
- ARN viral (génétique), Anticorps antiviraux (sang), Clonage moléculaire, Expression des gènes, Génome viral, Humains, Immunoglobuline G (sang), Protéines nucléocapside (génétique), Protéines nucléocapside (immunologie), Protéines nucléocapside (métabolisme), Protéines recombinantes (isolement et purification), Protéines recombinantes (métabolisme), RT-PCR, Syndrome respiratoire aigu sévère (diagnostic), Syndrome respiratoire aigu sévère (sang), Syndrome respiratoire aigu sévère (virologie), Technique de Western, Test ELISA, Virus du SRAS (génétique), Virus du SRAS (immunologie), Virus du SRAS (isolement et purification), Virus du SRAS (métabolisme).
- MESH :
- diagnostic : Syndrome respiratoire aigu sévère.
- génétique : ARN viral, Protéines nucléocapside, Virus du SRAS.
- immunologie : Protéines nucléocapside, Virus du SRAS.
- isolement et purification : Protéines recombinantes, Virus du SRAS.
- métabolisme : Protéines nucléocapside, Protéines recombinantes, Virus du SRAS.
- sang : Anticorps antiviraux, Immunoglobuline G, Syndrome respiratoire aigu sévère.
- virologie : Syndrome respiratoire aigu sévère.
- Clonage moléculaire, Expression des gènes, Génome viral, Humains, RT-PCR, Technique de Western, Test ELISA.
English descriptors
- KwdEn :
- Antibodies, Viral (blood), Blotting, Western, Cloning, Molecular, Enzyme-Linked Immunosorbent Assay, Gene Expression, Genome, Viral, Humans, Immunoglobulin G (blood), Nucleocapsid Proteins (genetics), Nucleocapsid Proteins (immunology), Nucleocapsid Proteins (metabolism), RNA, Viral (genetics), Recombinant Proteins (isolation & purification), Recombinant Proteins (metabolism), Reverse Transcriptase Polymerase Chain Reaction, SARS Virus (genetics), SARS Virus (immunology), SARS Virus (isolation & purification), SARS Virus (metabolism), Severe Acute Respiratory Syndrome (blood), Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (virology).
- MESH :
- chemical , blood : Antibodies, Viral, Immunoglobulin G.
- chemical , genetics : Nucleocapsid Proteins, RNA, Viral.
- chemical , immunology : Nucleocapsid Proteins.
- chemical , isolation & purification : Recombinant Proteins.
- chemical , metabolism : Nucleocapsid Proteins, Recombinant Proteins.
- blood : Severe Acute Respiratory Syndrome.
- diagnosis : Severe Acute Respiratory Syndrome.
- genetics : SARS Virus.
- immunology : SARS Virus.
- isolation & purification : SARS Virus.
- metabolism : SARS Virus.
- virology : Severe Acute Respiratory Syndrome.
- Blotting, Western, Cloning, Molecular, Enzyme-Linked Immunosorbent Assay, Gene Expression, Genome, Viral, Humans, Reverse Transcriptase Polymerase Chain Reaction.
Abstract
To clone and express nucleocapsid (N) protein of the severe acute respiratory syndrome (SARS)-associated coronavirus, and to evaluate its antigenicity and application value in the development of serological diagnostic test for SARS.
PubMed: 16201478
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pubmed:16201478Le document en format XML
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sort="Jiang, Xiao Ling" uniqKey="Jiang X" first="Xiao-Ling" last="Jiang">Xiao-Ling Jiang</name></author><author><name sortKey="Wen, Li Xia" sort="Wen, Li Xia" uniqKey="Wen L" first="Li-Xia" last="Wen">Li-Xia Wen</name></author><author><name sortKey="Le, Xiao Hua" sort="Le, Xiao Hua" uniqKey="Le X" first="Xiao-Hua" last="Le">Xiao-Hua Le</name></author><author><name sortKey="Li, Bing" sort="Li, Bing" uniqKey="Li B" first="Bing" last="Li">Bing Li</name></author><author><name sortKey="Xu, Liu Mei" sort="Xu, Liu Mei" uniqKey="Xu L" first="Liu-Mei" last="Xu">Liu-Mei Xu</name></author><author><name sortKey="Li, Li Xiong" sort="Li, Li Xiong" uniqKey="Li L" first="Li-Xiong" last="Li">Li-Xiong Li</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:16201478</idno><idno type="pmid">16201478</idno><idno type="wicri:Area/PubMed/Corpus">002517</idno><idno type="wicri:explorRef" wicri:stream="PubMed" 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type="city">Shenzhen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Zhou, Bai Ping" sort="Zhou, Bai Ping" uniqKey="Zhou B" first="Bai-Ping" last="Zhou">Bai-Ping Zhou</name></author><author><name sortKey="Zhou, Yu Sen" sort="Zhou, Yu Sen" uniqKey="Zhou Y" first="Yu-Sen" last="Zhou">Yu-Sen Zhou</name></author><author><name sortKey="Jiang, Xiao Ling" sort="Jiang, Xiao Ling" uniqKey="Jiang X" first="Xiao-Ling" last="Jiang">Xiao-Ling Jiang</name></author><author><name sortKey="Wen, Li Xia" sort="Wen, Li Xia" uniqKey="Wen L" first="Li-Xia" last="Wen">Li-Xia Wen</name></author><author><name sortKey="Le, Xiao Hua" sort="Le, Xiao Hua" uniqKey="Le X" first="Xiao-Hua" last="Le">Xiao-Hua Le</name></author><author><name sortKey="Li, Bing" sort="Li, Bing" uniqKey="Li B" first="Bing" last="Li">Bing Li</name></author><author><name sortKey="Xu, Liu Mei" sort="Xu, Liu Mei" uniqKey="Xu L" first="Liu-Mei" last="Xu">Liu-Mei Xu</name></author><author><name sortKey="Li, Li Xiong" sort="Li, Li Xiong" uniqKey="Li L" first="Li-Xiong" last="Li">Li-Xiong Li</name></author></analytic><series><title level="j">Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology</title><idno type="ISSN">1003-9279</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibodies, Viral (blood)</term><term>Blotting, Western</term><term>Cloning, Molecular</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Gene Expression</term><term>Genome, Viral</term><term>Humans</term><term>Immunoglobulin G (blood)</term><term>Nucleocapsid Proteins (genetics)</term><term>Nucleocapsid Proteins (immunology)</term><term>Nucleocapsid Proteins (metabolism)</term><term>RNA, Viral (genetics)</term><term>Recombinant Proteins (isolation & purification)</term><term>Recombinant Proteins (metabolism)</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>SARS Virus (genetics)</term><term>SARS Virus (immunology)</term><term>SARS Virus (isolation & purification)</term><term>SARS Virus (metabolism)</term><term>Severe Acute Respiratory Syndrome (blood)</term><term>Severe Acute Respiratory Syndrome (diagnosis)</term><term>Severe Acute Respiratory Syndrome (virology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (génétique)</term><term>Anticorps antiviraux (sang)</term><term>Clonage moléculaire</term><term>Expression des gènes</term><term>Génome viral</term><term>Humains</term><term>Immunoglobuline G (sang)</term><term>Protéines nucléocapside (génétique)</term><term>Protéines nucléocapside (immunologie)</term><term>Protéines nucléocapside (métabolisme)</term><term>Protéines recombinantes (isolement et purification)</term><term>Protéines recombinantes (métabolisme)</term><term>RT-PCR</term><term>Syndrome respiratoire aigu sévère (diagnostic)</term><term>Syndrome respiratoire aigu sévère (sang)</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Technique de Western</term><term>Test ELISA</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (immunologie)</term><term>Virus du SRAS (isolement et purification)</term><term>Virus du SRAS (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term><term>Immunoglobulin G</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Nucleocapsid Proteins</term><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Nucleocapsid Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Nucleocapsid Proteins</term><term>Recombinant Proteins</term></keywords><keywords scheme="MESH" qualifier="blood" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN viral</term><term>Protéines nucléocapside</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Protéines nucléocapside</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Protéines recombinantes</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines nucléocapside</term><term>Protéines recombinantes</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term><term>Immunoglobuline G</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Blotting, Western</term><term>Cloning, Molecular</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Gene Expression</term><term>Genome, Viral</term><term>Humans</term><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Clonage moléculaire</term><term>Expression des gènes</term><term>Génome viral</term><term>Humains</term><term>RT-PCR</term><term>Technique de Western</term><term>Test ELISA</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To clone and express nucleocapsid (N) protein of the severe acute respiratory syndrome (SARS)-associated coronavirus, and to evaluate its antigenicity and application value in the development of serological diagnostic test for SARS.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16201478</PMID><DateCompleted><Year>2009</Year><Month>01</Month><Day>05</Day></DateCompleted><DateRevised><Year>2005</Year><Month>10</Month><Day>05</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1003-9279</ISSN><JournalIssue CitedMedium="Print"><Volume>19</Volume><Issue>1</Issue><PubDate><Year>2005</Year><Month>Mar</Month></PubDate></JournalIssue><Title>Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology</Title><ISOAbbreviation>Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi</ISOAbbreviation></Journal><ArticleTitle>[Molecular cloning and expression of the severe acute respiratory syndrome-associated coronavirus nucleocapsid protein and its clinical application].</ArticleTitle><Pagination><MedlinePgn>64-7</MedlinePgn></Pagination><Abstract><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">To clone and express nucleocapsid (N) protein of the severe acute respiratory syndrome (SARS)-associated coronavirus, and to evaluate its antigenicity and application value in the development of serological diagnostic test for SARS.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">SARS-associated coronavirus N protein gene was amplified from its genomic RNA by reverse transcript nested polymerase chain reaction (RT-nested-PCR) and cloned into pBAD/Thio-TOPO prokaryotic expression vector. The recombinant N fusion protein was expressed and purified, and its antigenicity and specificity was analyzed by Western Blot, to establish the recombinant N protein-based ELISA for detection of IgG antibodies to SARS-associated coronavirus, and SARS-associated coronavirus lysates-based ELISA was compared parallelly.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">The recombinant expression vector produced high level of the N fusion protein after induction, and that protein was purified successfully by affinity chromatography and displayed higher antigenicity and specificity as compared with whole virus lysates.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">The recombinant SARS-associated coronavirus N protein possessed better antigenicity and specificity and could be employed to establish a new, sensitive, and specific ELISA for SARS diagnosis.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lu</LastName><ForeName>Jian</ForeName><Initials>J</Initials><AffiliationInfo><Affiliation>Shenzhen Donghu Hospital, Shenzhen, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Zhou</LastName><ForeName>Bai-ping</ForeName><Initials>BP</Initials></Author><Author ValidYN="Y"><LastName>Zhou</LastName><ForeName>Yu-sen</ForeName><Initials>YS</Initials></Author><Author ValidYN="Y"><LastName>Jiang</LastName><ForeName>Xiao-ling</ForeName><Initials>XL</Initials></Author><Author ValidYN="Y"><LastName>Wen</LastName><ForeName>Li-xia</ForeName><Initials>LX</Initials></Author><Author ValidYN="Y"><LastName>Le</LastName><ForeName>Xiao-hua</ForeName><Initials>XH</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Bing</ForeName><Initials>B</Initials></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Liu-mei</ForeName><Initials>LM</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Li-xiong</ForeName><Initials>LX</Initials></Author></AuthorList><Language>chi</Language><PublicationTypeList><PublicationType UI="D004740">English Abstract</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi</MedlineTA><NlmUniqueID>9602873</NlmUniqueID><ISSNLinking>1003-9279</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000914">Antibodies, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D007074">Immunoglobulin G</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D019590">Nucleocapsid Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D012367">RNA, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="C099602">nucleocapsid protein, Coronavirus</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000914" MajorTopicYN="N">Antibodies, Viral</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="N">blood</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015153" MajorTopicYN="N">Blotting, Western</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003001" MajorTopicYN="N">Cloning, Molecular</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004797" MajorTopicYN="N">Enzyme-Linked Immunosorbent Assay</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015870" MajorTopicYN="N">Gene Expression</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D016679" MajorTopicYN="N">Genome, Viral</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D007074" MajorTopicYN="N">Immunoglobulin G</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="N">blood</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D019590" MajorTopicYN="N">Nucleocapsid Proteins</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012367" MajorTopicYN="N">RNA, Viral</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D020133" MajorTopicYN="N">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName><QualifierName UI="Q000378" MajorTopicYN="N">metabolism</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045169" MajorTopicYN="N">Severe Acute Respiratory Syndrome</DescriptorName><QualifierName UI="Q000097" MajorTopicYN="N">blood</QualifierName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2005</Year><Month>10</Month><Day>6</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2009</Year><Month>1</Month><Day>6</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2005</Year><Month>10</Month><Day>6</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16201478</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Guangdong</li></region><settlement><li>Shenzhen</li></settlement></list><tree><noCountry><name sortKey="Jiang, Xiao Ling" sort="Jiang, Xiao Ling" uniqKey="Jiang X" first="Xiao-Ling" last="Jiang">Xiao-Ling Jiang</name><name sortKey="Le, Xiao Hua" sort="Le, Xiao Hua" uniqKey="Le X" first="Xiao-Hua" last="Le">Xiao-Hua Le</name><name sortKey="Li, Bing" sort="Li, Bing" uniqKey="Li B" first="Bing" last="Li">Bing Li</name><name sortKey="Li, Li Xiong" sort="Li, Li Xiong" uniqKey="Li L" first="Li-Xiong" last="Li">Li-Xiong Li</name><name sortKey="Wen, Li Xia" sort="Wen, Li Xia" uniqKey="Wen L" first="Li-Xia" last="Wen">Li-Xia Wen</name><name sortKey="Xu, Liu Mei" sort="Xu, Liu Mei" uniqKey="Xu L" first="Liu-Mei" last="Xu">Liu-Mei Xu</name><name sortKey="Zhou, Bai Ping" sort="Zhou, Bai Ping" uniqKey="Zhou B" first="Bai-Ping" last="Zhou">Bai-Ping Zhou</name><name sortKey="Zhou, Yu Sen" sort="Zhou, Yu Sen" uniqKey="Zhou Y" first="Yu-Sen" last="Zhou">Yu-Sen Zhou</name></noCountry><country name="République populaire de Chine"><region name="Guangdong"><name sortKey="Lu, Jian" sort="Lu, Jian" uniqKey="Lu J" first="Jian" last="Lu">Jian Lu</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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