A combined nucleocapsid vaccine induces vigorous SARS-CD8+ T-cell immune responses
Identifieur interne : 001127 ( Ncbi/Merge ); précédent : 001126; suivant : 001128A combined nucleocapsid vaccine induces vigorous SARS-CD8+ T-cell immune responses
Auteurs : Ali Azizi ; Susan Aucoin ; Helina Tadesse ; Rita Frost ; Masoud Ghorbani ; Catalina Soare ; Turaya Naas ; Francisco Diaz-MitomaSource :
- Genetic Vaccines and Therapy [ 1479-0556 ] ; 2005.
Abstract
Several studies have shown that cell-mediated immune responses play a crucial role in controlling viral replication. As such, a candidate SARS vaccine should elicit broad CD8+ T-cell immune responses. Several groups of mice were immunized alone or in combination with SARS-nucleocapsid immunogen. A high level of specific SARS-CD8+ T-cell response was demonstrated in mice that received DNA encoding the SARS-nucleocapsid, protein and XIAP as an adjuvant. We also observed that co-administration of a plasmid expressing nucleocapsid, recombinant protein and montanide/CpG induces high antibody titers in immunized mice. Moreover, this vaccine approach merits further investigation as a potential candidate vaccine against SARS.
The online version of this article (doi:10.1186/1479-0556-3-7) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1186/1479-0556-3-7
PubMed: 16115319
PubMed Central: 1249587
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Canada</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.28046.38</institution-id><institution-id institution-id-type="ISNI">0000000121822255</institution-id><institution>Department of Microbiology and Immunology, Faculty of Medicine,</institution><institution>University of Ottawa,</institution></institution-wrap>Ottawa, ON K1H 8M2 Canada</nlm:aff><wicri:noCountry code="subfield">ON K1H 8M2 Canada</wicri:noCountry></affiliation></author></analytic><series><title level="j">Genetic Vaccines and Therapy</title><idno type="eISSN">1479-0556</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Several studies have shown that cell-mediated immune responses play a crucial role in controlling viral replication. As such, a candidate SARS vaccine should elicit broad CD8+ T-cell immune responses. Several groups of mice were immunized alone or in combination with SARS-nucleocapsid immunogen. A high level of specific SARS-CD8+ T-cell response was demonstrated in mice that received DNA encoding the SARS-nucleocapsid, protein and XIAP as an adjuvant. We also observed that co-administration of a plasmid expressing nucleocapsid, recombinant protein and montanide/CpG induces high antibody titers in immunized mice. Moreover, this vaccine approach merits further investigation as a potential candidate vaccine against SARS.</p><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1186/1479-0556-3-7) contains supplementary material, which is available to authorized users.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Buchholz, Uj" uniqKey="Buchholz U">UJ Buchholz</name></author><author><name sortKey="Bukreyev, A" uniqKey="Bukreyev A">A Bukreyev</name></author><author><name sortKey="Yang, L" uniqKey="Yang L">L Yang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Spiga, O" uniqKey="Spiga O">O Spiga</name></author><author><name sortKey="Bernini, A" uniqKey="Bernini A">A Bernini</name></author><author><name sortKey="Ciutti, A" uniqKey="Ciutti A">A Ciutti</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lu, H" uniqKey="Lu H">H 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<front><journal-meta><journal-id journal-id-type="nlm-ta">Genet Vaccines Ther</journal-id><journal-id journal-id-type="iso-abbrev">Genet Vaccines Ther</journal-id><journal-title-group><journal-title>Genetic Vaccines and Therapy</journal-title></journal-title-group><issn pub-type="epub">1479-0556</issn><publisher><publisher-name>BioMed Central</publisher-name><publisher-loc>London</publisher-loc></publisher></journal-meta><article-meta><article-id pub-id-type="pmid">16115319</article-id><article-id pub-id-type="pmc">1249587</article-id><article-id pub-id-type="publisher-id">27</article-id><article-id pub-id-type="doi">10.1186/1479-0556-3-7</article-id><article-categories><subj-group subj-group-type="heading"><subject>Research</subject></subj-group></article-categories><title-group><article-title>A combined nucleocapsid vaccine induces vigorous SARS-CD8+ T-cell immune responses</article-title></title-group><contrib-group><contrib contrib-type="author"><name><surname>Azizi</surname><given-names>Ali</given-names></name><address><email>aliazizi555@yahoo.ca</email></address><xref ref-type="aff" rid="Aff1">1</xref><xref ref-type="aff" rid="Aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>Aucoin</surname><given-names>Susan</given-names></name><address><email>saucoin@cheo.on.ca</email></address><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Tadesse</surname><given-names>Helina</given-names></name><address><email>htade096@uottawa.ca</email></address><xref ref-type="aff" rid="Aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>Frost</surname><given-names>Rita</given-names></name><address><email>frost@cheo.on.ca</email></address><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Ghorbani</surname><given-names>Masoud</given-names></name><address><email>mghorbani@yahoo.com</email></address><xref ref-type="aff" rid="Aff1">1</xref></contrib><contrib contrib-type="author"><name><surname>Soare</surname><given-names>Catalina</given-names></name><address><email>catalinasoare@yahoo.com</email></address><xref ref-type="aff" rid="Aff1">1</xref><xref ref-type="aff" rid="Aff2">2</xref></contrib><contrib contrib-type="author"><name><surname>Naas</surname><given-names>Turaya</given-names></name><address><email>turayanaas@hotmail.com</email></address><xref ref-type="aff" rid="Aff1">1</xref><xref ref-type="aff" rid="Aff2">2</xref></contrib><contrib contrib-type="author" corresp="yes"><name><surname>Diaz-Mitoma</surname><given-names>Francisco</given-names></name><address><email>diaz@cheo.on.ca</email></address><xref ref-type="aff" rid="Aff1">1</xref><xref ref-type="aff" rid="Aff2">2</xref></contrib><aff id="Aff1"><label>1</label><institution-wrap><institution-id institution-id-type="GRID">grid.414148.c</institution-id><institution-id institution-id-type="ISNI">0000000094026172</institution-id><institution>Infectious Disease and Vaccine Research Centre,</institution><institution>Children's Hospital of Eastern Ontario Research Institute,</institution></institution-wrap>401 Smyth Road, Ottawa, ON K1H 8L1 Canada</aff><aff id="Aff2"><label>2</label><institution-wrap><institution-id institution-id-type="GRID">grid.28046.38</institution-id><institution-id institution-id-type="ISNI">0000000121822255</institution-id><institution>Department of Microbiology and Immunology, Faculty of Medicine,</institution><institution>University of Ottawa,</institution></institution-wrap>Ottawa, ON K1H 8M2 Canada</aff></contrib-group><pub-date pub-type="epub"><day>22</day><month>8</month><year>2005</year></pub-date><pub-date pub-type="pmc-release"><day>22</day><month>8</month><year>2005</year></pub-date><pub-date pub-type="collection"><year>2005</year></pub-date><volume>3</volume><elocation-id>7</elocation-id><history><date date-type="received"><day>09</day><month>5</month><year>2005</year></date><date date-type="accepted"><day>22</day><month>8</month><year>2005</year></date></history><permissions><copyright-statement>© Azizi et al; licensee BioMed Central Ltd. 2005</copyright-statement><license><license-p>This article is made available via the PMC Open Access Subset for unrestricted research re-use and secondary analysis in any form or by any means with acknowledgement of the original source. These permissions are granted for the duration of the World Health Organization (WHO) declaration of COVID-19 as a global pandemic.</license-p></license></permissions><abstract id="Abs1"><p>Several studies have shown that cell-mediated immune responses play a crucial role in controlling viral replication. As such, a candidate SARS vaccine should elicit broad CD8+ T-cell immune responses. Several groups of mice were immunized alone or in combination with SARS-nucleocapsid immunogen. A high level of specific SARS-CD8+ T-cell response was demonstrated in mice that received DNA encoding the SARS-nucleocapsid, protein and XIAP as an adjuvant. We also observed that co-administration of a plasmid expressing nucleocapsid, recombinant protein and montanide/CpG induces high antibody titers in immunized mice. Moreover, this vaccine approach merits further investigation as a potential candidate vaccine against SARS.</p><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1186/1479-0556-3-7) contains supplementary material, which is available to authorized users.</p></sec></abstract><kwd-group xml:lang="en"><title>Keywords</title><kwd>Vaccine</kwd><kwd>SARS</kwd><kwd>Nucleocapsid</kwd><kwd>XIAP</kwd></kwd-group><custom-meta-group><custom-meta><meta-name>issue-copyright-statement</meta-name><meta-value>© BioMed Central Ltd 2005</meta-value></custom-meta></custom-meta-group></article-meta></front></pmc><affiliations><list></list><tree><noCountry><name sortKey="Aucoin, Susan" sort="Aucoin, Susan" uniqKey="Aucoin S" first="Susan" last="Aucoin">Susan Aucoin</name><name sortKey="Azizi, Ali" sort="Azizi, Ali" uniqKey="Azizi A" first="Ali" last="Azizi">Ali Azizi</name><name sortKey="Diaz Mitoma, Francisco" sort="Diaz Mitoma, Francisco" uniqKey="Diaz Mitoma F" first="Francisco" last="Diaz-Mitoma">Francisco Diaz-Mitoma</name><name sortKey="Frost, Rita" sort="Frost, Rita" uniqKey="Frost R" first="Rita" last="Frost">Rita Frost</name><name sortKey="Ghorbani, Masoud" sort="Ghorbani, Masoud" uniqKey="Ghorbani M" first="Masoud" last="Ghorbani">Masoud Ghorbani</name><name sortKey="Naas, Turaya" sort="Naas, Turaya" uniqKey="Naas T" first="Turaya" last="Naas">Turaya Naas</name><name sortKey="Soare, Catalina" sort="Soare, Catalina" uniqKey="Soare C" first="Catalina" last="Soare">Catalina Soare</name><name sortKey="Tadesse, Helina" sort="Tadesse, Helina" uniqKey="Tadesse H" first="Helina" last="Tadesse">Helina Tadesse</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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