SrasV1, Ncbi, Merge, bibRecord, 001109

Construction of a eukaryotic expression plasmid encoding partial S gene fragments of the SARS-CoV and its potential utility as a DNA vaccine.

Identifieur interne : 001109 ( Ncbi/Merge ); précédent : 001108; suivant : 001110

Construction of a eukaryotic expression plasmid encoding partial S gene fragments of the SARS-CoV and its potential utility as a DNA vaccine.

Auteurs : Hongxuan He [République populaire de Chine] ; Yi Tang ; Ximing Qin ; Wenbo Xu ; Yifei Wang ; Xiangjun Liu ; Xingyou Liu ; Sheng Xiong ; Jiuxiang Li ; Meiying Zhang ; Mingxing Duan

Source :

DNA and cell biology [ 1044-5498 ] ; 2005.

RBID : pubmed:16101350

Descripteurs français

English descriptors

KwdEn :
- Animals, Antibodies, Viral (blood), CD4-CD8 Ratio, Cell Proliferation, Female, Immunoglobulin G (blood), Interferon-gamma (blood), Interleukin-4 (blood), Lymphocyte Count, Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Mice, Mice, Inbred BALB C, Plasmids, Recombinant Proteins, SARS Virus (genetics), SARS Virus (immunology), Spike Glycoprotein, Coronavirus, T-Lymphocytes (immunology), Vaccines, DNA, Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology), Viral Vaccines.
MESH :
- chemical , blood : Antibodies, Viral, Immunoglobulin G, Interferon-gamma, Interleukin-4.
- chemical , genetics : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , immunology : Membrane Glycoproteins, Viral Envelope Proteins.
- genetics : SARS Virus.
- immunology : SARS Virus, T-Lymphocytes.
- Animals, CD4-CD8 Ratio, Cell Proliferation, Female, Lymphocyte Count, Mice, Mice, Inbred BALB C, Plasmids, Recombinant Proteins, Spike Glycoprotein, Coronavirus, Vaccines, DNA, Viral Vaccines.

Abstract

The spike (S) protein, a main surface antigen of the SARS coronavirus (SARS-CoV), is considered to be one of the most important protective antigen candidates for targets for vaccine design against the virus. In this study, a secreted recombinant expression plasmid, pVAX-S1, encoding the partial S protein with a signal peptide, was constructed and used to immunize BALB/c mice through electroporation. It was demonstrated that the eukaryotic expression vector pVAX-S1 was successfully constructed by restriction enzyme and sequence analysis. The expressed protein could be detected specifically by Western blot analysis. The serum IgG level of the vaccine group mice was significantly higher than that of the corresponding control group at day 14 after vaccination (P < 0.05). The vaccine group demonstrated significantly higher S1 protein lymphocyte proliferation index (LPI) than the control groups (P < 0.05). Furthermore, in the experimental group, a decrease in the ratio of CD4(+) to CD8(+) T-lymphocytes and an increase level of IFN-gamma in serum were observed. However, interleukin-4 (IL-4) was not detectable in two groups. These results strongly demonstrated that the pVAX-S1 plasmid could induce humoral and cellular immune responses in mice, and may be a potential candidate for a DNA vaccine against the SARS coronavirus.

DOI: 10.1089/dna.2005.24.516
PubMed: 16101350

Links toward previous steps (curation, corpus...)

to stream PubMed, to step Corpus: 002591
to stream PubMed, to step Curation: 002591
to stream PubMed, to step Checkpoint: 002782

Links to Exploration step

pubmed:16101350

Le document en format XML

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<front><div type="abstract" xml:lang="en">The spike (S) protein, a main surface antigen of the SARS coronavirus (SARS-CoV), is considered to be one of the most important protective antigen candidates for targets for vaccine design against the virus. In this study, a secreted recombinant expression plasmid, pVAX-S1, encoding the partial S protein with a signal peptide, was constructed and used to immunize BALB/c mice through electroporation. It was demonstrated that the eukaryotic expression vector pVAX-S1 was successfully constructed by restriction enzyme and sequence analysis. The expressed protein could be detected specifically by Western blot analysis. The serum IgG level of the vaccine group mice was significantly higher than that of the corresponding control group at day 14 after vaccination (P < 0.05). The vaccine group demonstrated significantly higher S1 protein lymphocyte proliferation index (LPI) than the control groups (P < 0.05). Furthermore, in the experimental group, a decrease in the ratio of CD4(+) to CD8(+) T-lymphocytes and an increase level of IFN-gamma in serum were observed. However, interleukin-4 (IL-4) was not detectable in two groups. These results strongly demonstrated that the pVAX-S1 plasmid could induce humoral and cellular immune responses in mice, and may be a potential candidate for a DNA vaccine against the SARS coronavirus.</div>
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<Abstract><AbstractText>The spike (S) protein, a main surface antigen of the SARS coronavirus (SARS-CoV), is considered to be one of the most important protective antigen candidates for targets for vaccine design against the virus. In this study, a secreted recombinant expression plasmid, pVAX-S1, encoding the partial S protein with a signal peptide, was constructed and used to immunize BALB/c mice through electroporation. It was demonstrated that the eukaryotic expression vector pVAX-S1 was successfully constructed by restriction enzyme and sequence analysis. The expressed protein could be detected specifically by Western blot analysis. The serum IgG level of the vaccine group mice was significantly higher than that of the corresponding control group at day 14 after vaccination (P < 0.05). The vaccine group demonstrated significantly higher S1 protein lymphocyte proliferation index (LPI) than the control groups (P < 0.05). Furthermore, in the experimental group, a decrease in the ratio of CD4(+) to CD8(+) T-lymphocytes and an increase level of IFN-gamma in serum were observed. However, interleukin-4 (IL-4) was not detectable in two groups. These results strongly demonstrated that the pVAX-S1 plasmid could induce humoral and cellular immune responses in mice, and may be a potential candidate for a DNA vaccine against the SARS coronavirus.</AbstractText>
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<tree><noCountry><name sortKey="Duan, Mingxing" sort="Duan, Mingxing" uniqKey="Duan M" first="Mingxing" last="Duan">Mingxing Duan</name>
<name sortKey="Li, Jiuxiang" sort="Li, Jiuxiang" uniqKey="Li J" first="Jiuxiang" last="Li">Jiuxiang Li</name>
<name sortKey="Liu, Xiangjun" sort="Liu, Xiangjun" uniqKey="Liu X" first="Xiangjun" last="Liu">Xiangjun Liu</name>
<name sortKey="Liu, Xingyou" sort="Liu, Xingyou" uniqKey="Liu X" first="Xingyou" last="Liu">Xingyou Liu</name>
<name sortKey="Qin, Ximing" sort="Qin, Ximing" uniqKey="Qin X" first="Ximing" last="Qin">Ximing Qin</name>
<name sortKey="Tang, Yi" sort="Tang, Yi" uniqKey="Tang Y" first="Yi" last="Tang">Yi Tang</name>
<name sortKey="Wang, Yifei" sort="Wang, Yifei" uniqKey="Wang Y" first="Yifei" last="Wang">Yifei Wang</name>
<name sortKey="Xiong, Sheng" sort="Xiong, Sheng" uniqKey="Xiong S" first="Sheng" last="Xiong">Sheng Xiong</name>
<name sortKey="Xu, Wenbo" sort="Xu, Wenbo" uniqKey="Xu W" first="Wenbo" last="Xu">Wenbo Xu</name>
<name sortKey="Zhang, Meiying" sort="Zhang, Meiying" uniqKey="Zhang M" first="Meiying" last="Zhang">Meiying Zhang</name>
</noCountry>
<country name="République populaire de Chine"><noRegion><name sortKey="He, Hongxuan" sort="He, Hongxuan" uniqKey="He H" first="Hongxuan" last="He">Hongxuan He</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>

Pour manipuler ce document sous Unix (Dilib)

EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Ncbi/Merge

HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001109 | SxmlIndent | more

HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd -nk 001109 | SxmlIndent | more

Pour mettre un lien sur cette page dans le réseau Wicri

{{Explor lien
   |wiki=    Sante
   |area=    SrasV1
   |flux=    Ncbi
   |étape=   Merge
   |type=    RBID
   |clé=     pubmed:16101350
   |texte=   Construction of a eukaryotic expression plasmid encoding partial S gene fragments of the SARS-CoV and its potential utility as a DNA vaccine.
}}

Pour générer des pages wiki

HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/RBID.i   -Sk "pubmed:16101350" \
       | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd   \
       | NlmPubMed2Wicri -a SrasV1

This area was generated with Dilib version V0.6.33.
Data generation: Tue Apr 28 14:49:16 2020. Site generation: Sat Mar 27 22:06:49 2021

	Serveur d'exploration SRAS
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Serveur d'exploration SRAS

Construction of a eukaryotic expression plasmid encoding partial S gene fragments of the SARS-CoV and its potential utility as a DNA vaccine.

Construction of a eukaryotic expression plasmid encoding partial S gene fragments of the SARS-CoV and its potential utility as a DNA vaccine.

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Pour mettre un lien sur cette page dans le réseau Wicri

Pour générer des pages wiki