[Conditions for SARS-CoV cultivation and inactivation].
Identifieur interne : 001068 ( Ncbi/Merge ); précédent : 001067; suivant : 001069[Conditions for SARS-CoV cultivation and inactivation].
Auteurs : Song-Le Zhang [République populaire de Chine] ; Li-Juan Ma ; Guang Tian ; Liang-Yan Zhang ; Xue-Ying Zhang ; Xi-Liang WangSource :
- Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology [ 1003-9279 ] ; 2005.
Descripteurs français
- KwdFr :
- MESH :
- croissance et développement : Virus du SRAS.
- pharmacologie : Propiolactone.
- Animaux, Cellules Vero, Facteurs temps, Inactivation virale, Relation dose-effet des médicaments, Température, Virus du SRAS.
English descriptors
- KwdEn :
- MESH :
- chemical , pharmacology : Propiolactone.
- drug effects : SARS Virus, Virus Inactivation.
- growth & development : SARS Virus.
- Animals, Chlorocebus aethiops, Dose-Response Relationship, Drug, Temperature, Time Factors, Vero Cells.
Abstract
To study the method for cultivation and inactivation of SARS-CoV.
PubMed: 16027779
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 002633
- to stream PubMed, to step Curation: 002633
- to stream PubMed, to step Checkpoint: 002385
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pubmed:16027779Le document en format XML
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<author><name sortKey="Zhang, Song Le" sort="Zhang, Song Le" uniqKey="Zhang S" first="Song-Le" last="Zhang">Song-Le Zhang</name>
<affiliation wicri:level="1"><nlm:affiliation>Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China. zhangsl@163.net</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071</wicri:regionArea>
<placeName><settlement type="city">Pékin</settlement>
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<author><name sortKey="Ma, Li Juan" sort="Ma, Li Juan" uniqKey="Ma L" first="Li-Juan" last="Ma">Li-Juan Ma</name>
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<author><name sortKey="Tian, Guang" sort="Tian, Guang" uniqKey="Tian G" first="Guang" last="Tian">Guang Tian</name>
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<author><name sortKey="Zhang, Liang Yan" sort="Zhang, Liang Yan" uniqKey="Zhang L" first="Liang-Yan" last="Zhang">Liang-Yan Zhang</name>
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<author><name sortKey="Zhang, Xue Ying" sort="Zhang, Xue Ying" uniqKey="Zhang X" first="Xue-Ying" last="Zhang">Xue-Ying Zhang</name>
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<author><name sortKey="Wang, Xi Liang" sort="Wang, Xi Liang" uniqKey="Wang X" first="Xi-Liang" last="Wang">Xi-Liang Wang</name>
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<author><name sortKey="Zhang, Song Le" sort="Zhang, Song Le" uniqKey="Zhang S" first="Song-Le" last="Zhang">Song-Le Zhang</name>
<affiliation wicri:level="1"><nlm:affiliation>Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China. zhangsl@163.net</nlm:affiliation>
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<author><name sortKey="Ma, Li Juan" sort="Ma, Li Juan" uniqKey="Ma L" first="Li-Juan" last="Ma">Li-Juan Ma</name>
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<author><name sortKey="Tian, Guang" sort="Tian, Guang" uniqKey="Tian G" first="Guang" last="Tian">Guang Tian</name>
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<author><name sortKey="Zhang, Liang Yan" sort="Zhang, Liang Yan" uniqKey="Zhang L" first="Liang-Yan" last="Zhang">Liang-Yan Zhang</name>
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<author><name sortKey="Zhang, Xue Ying" sort="Zhang, Xue Ying" uniqKey="Zhang X" first="Xue-Ying" last="Zhang">Xue-Ying Zhang</name>
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<author><name sortKey="Wang, Xi Liang" sort="Wang, Xi Liang" uniqKey="Wang X" first="Xi-Liang" last="Wang">Xi-Liang Wang</name>
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<series><title level="j">Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology</title>
<idno type="ISSN">1003-9279</idno>
<imprint><date when="2005" type="published">2005</date>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Chlorocebus aethiops</term>
<term>Dose-Response Relationship, Drug</term>
<term>Propiolactone (pharmacology)</term>
<term>SARS Virus (drug effects)</term>
<term>SARS Virus (growth & development)</term>
<term>Temperature</term>
<term>Time Factors</term>
<term>Vero Cells</term>
<term>Virus Inactivation (drug effects)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term>
<term>Cellules Vero</term>
<term>Facteurs temps</term>
<term>Inactivation virale ()</term>
<term>Propiolactone (pharmacologie)</term>
<term>Relation dose-effet des médicaments</term>
<term>Température</term>
<term>Virus du SRAS ()</term>
<term>Virus du SRAS (croissance et développement)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Propiolactone</term>
</keywords>
<keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>SARS Virus</term>
<term>Virus Inactivation</term>
</keywords>
<keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Propiolactone</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Chlorocebus aethiops</term>
<term>Dose-Response Relationship, Drug</term>
<term>Temperature</term>
<term>Time Factors</term>
<term>Vero Cells</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Cellules Vero</term>
<term>Facteurs temps</term>
<term>Inactivation virale</term>
<term>Relation dose-effet des médicaments</term>
<term>Température</term>
<term>Virus du SRAS</term>
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<front><div type="abstract" xml:lang="en">To study the method for cultivation and inactivation of SARS-CoV.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16027779</PMID>
<DateCompleted><Year>2008</Year>
<Month>12</Month>
<Day>22</Day>
</DateCompleted>
<DateRevised><Year>2019</Year>
<Month>12</Month>
<Day>10</Day>
</DateRevised>
<Article PubModel="Print"><Journal><ISSN IssnType="Print">1003-9279</ISSN>
<JournalIssue CitedMedium="Print"><Volume>19</Volume>
<Issue>2</Issue>
<PubDate><Year>2005</Year>
<Month>Jun</Month>
</PubDate>
</JournalIssue>
<Title>Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology</Title>
<ISOAbbreviation>Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi</ISOAbbreviation>
</Journal>
<ArticleTitle>[Conditions for SARS-CoV cultivation and inactivation].</ArticleTitle>
<Pagination><MedlinePgn>135-7</MedlinePgn>
</Pagination>
<Abstract><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">To study the method for cultivation and inactivation of SARS-CoV.</AbstractText>
<AbstractText Label="METHODS" NlmCategory="METHODS">In order to choose the sensitive cell strain and the best infection dose of the virus, Vero, Vero-E6 and 2BS cell lines were infected with SARS-CoV. The cultivation temperature was selected among 25 degrees C, 33 degrees C and 37 degrees C. The best inactivating time and effect were observed with beta-propiolactone whose concentration ranged from 1:2000 to 1:20,000 at room temperature.</AbstractText>
<AbstractText Label="RESULTS" NlmCategory="RESULTS">Vero and Vero-E6 cell lines were sensitive to SARS-CoV. The cytopathic changes of the cells were 75% at 37 degrees C in 5 percent CO2 incubator after infection. SARS-CoV was inactivated completely in beta-propiolactone (1:4000). The toxicity of beta-propiolactone was hydrolyzed completely when the inactivated virus was cultured for 16 hours at 2 degrees C, 8 degrees C and in water bath for 2 hours at 37 degrees C.</AbstractText>
<AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">The titer of SARS-CoV was the highest when it was cultured in Vero or Vero-E6 cells for 72 hours at 37 degrees C in 5 percent CO2 incubator. SARS-CoV was inactivated completely in beta-propiolactone when its concentration was 1:4000 and the interaction time was 1 hour.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhang</LastName>
<ForeName>Song-le</ForeName>
<Initials>SL</Initials>
<AffiliationInfo><Affiliation>Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China. zhangsl@163.net</Affiliation>
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<Author ValidYN="Y"><LastName>Ma</LastName>
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<Author ValidYN="Y"><LastName>Zhang</LastName>
<ForeName>Xue-ying</ForeName>
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<Author ValidYN="Y"><LastName>Wang</LastName>
<ForeName>Xi-liang</ForeName>
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<MedlineJournalInfo><Country>China</Country>
<MedlineTA>Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi</MedlineTA>
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<ISSNLinking>1003-9279</ISSNLinking>
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<ChemicalList><Chemical><RegistryNumber>6RC3ZT4HB0</RegistryNumber>
<NameOfSubstance UI="D011420">Propiolactone</NameOfSubstance>
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<MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName>
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<MeshHeading><DescriptorName UI="D002522" MajorTopicYN="N">Chlorocebus aethiops</DescriptorName>
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<MeshHeading><DescriptorName UI="D004305" MajorTopicYN="N">Dose-Response Relationship, Drug</DescriptorName>
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<QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName>
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<MeshHeading><DescriptorName UI="D013696" MajorTopicYN="N">Temperature</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013997" MajorTopicYN="N">Time Factors</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014709" MajorTopicYN="N">Vero Cells</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D038301" MajorTopicYN="N">Virus Inactivation</DescriptorName>
<QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName>
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