[Conditions for SARS-CoV cultivation and inactivation].
Identifieur interne : 001068 ( Ncbi/Merge ); précédent : 001067; suivant : 001069[Conditions for SARS-CoV cultivation and inactivation].
Auteurs : Song-Le Zhang [République populaire de Chine] ; Li-Juan Ma ; Guang Tian ; Liang-Yan Zhang ; Xue-Ying Zhang ; Xi-Liang WangSource :
- Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology [ 1003-9279 ] ; 2005.
Descripteurs français
- KwdFr :
- MESH :
- croissance et développement : Virus du SRAS.
- pharmacologie : Propiolactone.
- Animaux, Cellules Vero, Facteurs temps, Inactivation virale, Relation dose-effet des médicaments, Température, Virus du SRAS.
English descriptors
- KwdEn :
- MESH :
- chemical , pharmacology : Propiolactone.
- drug effects : SARS Virus, Virus Inactivation.
- growth & development : SARS Virus.
- Animals, Chlorocebus aethiops, Dose-Response Relationship, Drug, Temperature, Time Factors, Vero Cells.
Abstract
To study the method for cultivation and inactivation of SARS-CoV.
PubMed: 16027779
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pubmed:16027779Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">[Conditions for SARS-CoV cultivation and inactivation].</title><author><name sortKey="Zhang, Song Le" sort="Zhang, Song Le" uniqKey="Zhang S" first="Song-Le" last="Zhang">Song-Le Zhang</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China. zhangsl@163.net</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Ma, Li Juan" sort="Ma, Li Juan" uniqKey="Ma L" first="Li-Juan" last="Ma">Li-Juan Ma</name></author><author><name sortKey="Tian, Guang" sort="Tian, Guang" uniqKey="Tian G" first="Guang" last="Tian">Guang Tian</name></author><author><name sortKey="Zhang, Liang Yan" sort="Zhang, Liang Yan" uniqKey="Zhang L" first="Liang-Yan" last="Zhang">Liang-Yan Zhang</name></author><author><name sortKey="Zhang, Xue Ying" sort="Zhang, Xue Ying" uniqKey="Zhang X" first="Xue-Ying" last="Zhang">Xue-Ying Zhang</name></author><author><name sortKey="Wang, Xi Liang" sort="Wang, Xi Liang" uniqKey="Wang X" first="Xi-Liang" last="Wang">Xi-Liang Wang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:16027779</idno><idno type="pmid">16027779</idno><idno type="wicri:Area/PubMed/Corpus">002633</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002633</idno><idno type="wicri:Area/PubMed/Curation">002633</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002633</idno><idno type="wicri:Area/PubMed/Checkpoint">002385</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002385</idno><idno type="wicri:Area/Ncbi/Merge">001068</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">[Conditions for SARS-CoV cultivation and inactivation].</title><author><name sortKey="Zhang, Song Le" sort="Zhang, Song Le" uniqKey="Zhang S" first="Song-Le" last="Zhang">Song-Le Zhang</name><affiliation wicri:level="1"><nlm:affiliation>Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China. zhangsl@163.net</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Ma, Li Juan" sort="Ma, Li Juan" uniqKey="Ma L" first="Li-Juan" last="Ma">Li-Juan Ma</name></author><author><name sortKey="Tian, Guang" sort="Tian, Guang" uniqKey="Tian G" first="Guang" last="Tian">Guang Tian</name></author><author><name sortKey="Zhang, Liang Yan" sort="Zhang, Liang Yan" uniqKey="Zhang L" first="Liang-Yan" last="Zhang">Liang-Yan Zhang</name></author><author><name sortKey="Zhang, Xue Ying" sort="Zhang, Xue Ying" uniqKey="Zhang X" first="Xue-Ying" last="Zhang">Xue-Ying Zhang</name></author><author><name sortKey="Wang, Xi Liang" sort="Wang, Xi Liang" uniqKey="Wang X" first="Xi-Liang" last="Wang">Xi-Liang Wang</name></author></analytic><series><title level="j">Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology</title><idno type="ISSN">1003-9279</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Dose-Response Relationship, Drug</term><term>Propiolactone (pharmacology)</term><term>SARS Virus (drug effects)</term><term>SARS Virus (growth & development)</term><term>Temperature</term><term>Time Factors</term><term>Vero Cells</term><term>Virus Inactivation (drug effects)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Facteurs temps</term><term>Inactivation virale ()</term><term>Propiolactone (pharmacologie)</term><term>Relation dose-effet des médicaments</term><term>Température</term><term>Virus du SRAS ()</term><term>Virus du SRAS (croissance et développement)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Propiolactone</term></keywords><keywords scheme="MESH" qualifier="croissance et développement" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>SARS Virus</term><term>Virus Inactivation</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Propiolactone</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Dose-Response Relationship, Drug</term><term>Temperature</term><term>Time Factors</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Facteurs temps</term><term>Inactivation virale</term><term>Relation dose-effet des médicaments</term><term>Température</term><term>Virus du SRAS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To study the method for cultivation and inactivation of SARS-CoV.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">16027779</PMID><DateCompleted><Year>2008</Year><Month>12</Month><Day>22</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1003-9279</ISSN><JournalIssue CitedMedium="Print"><Volume>19</Volume><Issue>2</Issue><PubDate><Year>2005</Year><Month>Jun</Month></PubDate></JournalIssue><Title>Zhonghua shi yan he lin chuang bing du xue za zhi = Zhonghua shiyan he linchuang bingduxue zazhi = Chinese journal of experimental and clinical virology</Title><ISOAbbreviation>Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi</ISOAbbreviation></Journal><ArticleTitle>[Conditions for SARS-CoV cultivation and inactivation].</ArticleTitle><Pagination><MedlinePgn>135-7</MedlinePgn></Pagination><Abstract><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">To study the method for cultivation and inactivation of SARS-CoV.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">In order to choose the sensitive cell strain and the best infection dose of the virus, Vero, Vero-E6 and 2BS cell lines were infected with SARS-CoV. The cultivation temperature was selected among 25 degrees C, 33 degrees C and 37 degrees C. The best inactivating time and effect were observed with beta-propiolactone whose concentration ranged from 1:2000 to 1:20,000 at room temperature.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">Vero and Vero-E6 cell lines were sensitive to SARS-CoV. The cytopathic changes of the cells were 75% at 37 degrees C in 5 percent CO2 incubator after infection. SARS-CoV was inactivated completely in beta-propiolactone (1:4000). The toxicity of beta-propiolactone was hydrolyzed completely when the inactivated virus was cultured for 16 hours at 2 degrees C, 8 degrees C and in water bath for 2 hours at 37 degrees C.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">The titer of SARS-CoV was the highest when it was cultured in Vero or Vero-E6 cells for 72 hours at 37 degrees C in 5 percent CO2 incubator. SARS-CoV was inactivated completely in beta-propiolactone when its concentration was 1:4000 and the interaction time was 1 hour.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Song-le</ForeName><Initials>SL</Initials><AffiliationInfo><Affiliation>Institute of Microbiology and Epidemiology, Academy of Military Medical Sciences, Beijing 100071, China. zhangsl@163.net</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Ma</LastName><ForeName>Li-juan</ForeName><Initials>LJ</Initials></Author><Author ValidYN="Y"><LastName>Tian</LastName><ForeName>Guang</ForeName><Initials>G</Initials></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Liang-yan</ForeName><Initials>LY</Initials></Author><Author ValidYN="Y"><LastName>Zhang</LastName><ForeName>Xue-ying</ForeName><Initials>XY</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Xi-liang</ForeName><Initials>XL</Initials></Author></AuthorList><Language>chi</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi</MedlineTA><NlmUniqueID>9602873</NlmUniqueID><ISSNLinking>1003-9279</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>6RC3ZT4HB0</RegistryNumber><NameOfSubstance UI="D011420">Propiolactone</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002522" MajorTopicYN="N">Chlorocebus aethiops</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004305" MajorTopicYN="N">Dose-Response Relationship, Drug</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011420" MajorTopicYN="N">Propiolactone</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName><QualifierName UI="Q000254" MajorTopicYN="Y">growth & development</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013696" MajorTopicYN="N">Temperature</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D013997" MajorTopicYN="N">Time Factors</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014709" MajorTopicYN="N">Vero Cells</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D038301" MajorTopicYN="N">Virus Inactivation</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2005</Year><Month>7</Month><Day>20</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2008</Year><Month>12</Month><Day>23</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2005</Year><Month>7</Month><Day>20</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">16027779</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><noCountry><name sortKey="Ma, Li Juan" sort="Ma, Li Juan" uniqKey="Ma L" first="Li-Juan" last="Ma">Li-Juan Ma</name><name sortKey="Tian, Guang" sort="Tian, Guang" uniqKey="Tian G" first="Guang" last="Tian">Guang Tian</name><name sortKey="Wang, Xi Liang" sort="Wang, Xi Liang" uniqKey="Wang X" first="Xi-Liang" last="Wang">Xi-Liang Wang</name><name sortKey="Zhang, Liang Yan" sort="Zhang, Liang Yan" uniqKey="Zhang L" first="Liang-Yan" last="Zhang">Liang-Yan Zhang</name><name sortKey="Zhang, Xue Ying" sort="Zhang, Xue Ying" uniqKey="Zhang X" first="Xue-Ying" last="Zhang">Xue-Ying Zhang</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Zhang, Song Le" sort="Zhang, Song Le" uniqKey="Zhang S" first="Song-Le" last="Zhang">Song-Le Zhang</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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