Isolation of Virus from a SARS Patient and Genome-wide Analysis of Genetic Mutations Related to Pathogenesis and Epidemiology from 47 SARS-CoV Isolates
Identifieur interne : 000E09 ( Ncbi/Merge ); précédent : 000E08; suivant : 000E10Isolation of Virus from a SARS Patient and Genome-wide Analysis of Genetic Mutations Related to Pathogenesis and Epidemiology from 47 SARS-CoV Isolates
Auteurs : Ying Zhu ; Mo Liu ; Weiguang Zhao ; Jianlin Zhang ; Xue Zhang ; Ke Wang ; Chunfang Gu ; Kailang Wu ; Yan Li ; Congyi Zheng ; Gengfu Xiao ; Huimin Yan ; Jiamin Zhang ; Deyin Guo ; Po Tien ; Jianguo WuSource :
- Virus Genes [ 0920-8569 ] ; 2005.
Descripteurs français
- KwdFr :
- ADN complémentaire (), ADN complémentaire (isolement et purification), ARN viral (analyse), ARN viral (génétique), Adolescent, Analyse de séquence, Chine (épidémiologie), Données de séquences moléculaires, Génome viral, Humains, Microscopie électronique, Mutation, Mutation faux-sens, Mutation ponctuelle, Mâle, RT-PCR, Syndrome respiratoire aigu sévère (virologie), Syndrome respiratoire aigu sévère (épidémiologie), Virulence (génétique), Virus du SRAS (génétique), Virus du SRAS (isolement et purification), Virus du SRAS (pathogénicité), Épidémiologie moléculaire, Évolution moléculaire.
- MESH :
- analyse : ARN viral.
- génétique : ARN viral, Virulence, Virus du SRAS.
- isolement et purification : ADN complémentaire, Virus du SRAS.
- pathogénicité : Virus du SRAS.
- virologie : Syndrome respiratoire aigu sévère.
- épidémiologie : Chine, Syndrome respiratoire aigu sévère.
- ADN complémentaire, Adolescent, Analyse de séquence, Données de séquences moléculaires, Génome viral, Humains, Microscopie électronique, Mutation, Mutation faux-sens, Mutation ponctuelle, Mâle, RT-PCR, Épidémiologie moléculaire, Évolution moléculaire.
- Wicri :
- geographic : République populaire de Chine.
English descriptors
- KwdEn :
- Adolescent, China (epidemiology), DNA, Complementary (chemistry), DNA, Complementary (isolation & purification), Evolution, Molecular, Genome, Viral, Humans, Male, Microscopy, Electron, Molecular Epidemiology, Molecular Sequence Data, Mutation, Mutation, Missense, Point Mutation, RNA, Viral (analysis), RNA, Viral (genetics), Reverse Transcriptase Polymerase Chain Reaction, SARS Virus (genetics), SARS Virus (isolation & purification), SARS Virus (pathogenicity), Sequence Analysis, Severe Acute Respiratory Syndrome (epidemiology), Severe Acute Respiratory Syndrome (virology), Virulence (genetics).
- MESH :
- chemical , analysis : RNA, Viral.
- chemical , chemistry : DNA, Complementary.
- geographic , epidemiology : China.
- chemical , genetics : RNA, Viral.
- chemical , isolation & purification : DNA, Complementary.
- epidemiology : Severe Acute Respiratory Syndrome.
- genetics : SARS Virus, Virulence.
- isolation & purification : SARS Virus.
- pathogenicity : SARS Virus.
- virology : Severe Acute Respiratory Syndrome.
- Adolescent, Evolution, Molecular, Genome, Viral, Humans, Male, Microscopy, Electron, Molecular Epidemiology, Molecular Sequence Data, Mutation, Mutation, Missense, Point Mutation, Reverse Transcriptase Polymerase Chain Reaction, Sequence Analysis.
Abstract
Severe acute respiratory syndrome (SARS) caused by SARS-associated coronavirus (SARS-CoV) is a fatal disease. Prevention of future outbreaks is essential and requires understanding pathogenesis and evolution of the virus. We have isolated a SARS-CoV in China and analyzed 47 SARS-CoV genomes with the aims to reveal the evolution trends of the virus and provide insights into understanding pathogenesis and SARS epidemic. Specimen from a SARS patient was inoculated into cell culture. The presence of SARS-CoV was determined by RT-PCR and confirmed by electron microscopy. Virus was isolated followed by the determination of its genome sequences, which were then analyzed by comparing with other 46 SARS-CoV genomes. Genetic mutations with potential implications to pathogenesis and the epidemic were characterized. This viral genome consists of 29,728 nucleotides with overall organization in agreement with that of published isolates. A total of 348 positions were mutated on 47 viral genomes. Among them 22 had mutations in more than three genomes. Hot spots of nucleotide variations and unique trends of mutations were identified on the viral genomes. Mutation rates were different from gene to gene and were correlated well with periodical or geographic characteristics of the epidemic.
Url:
DOI: 10.1007/s11262-004-4586-9
PubMed: 15744567
PubMed Central: 7089183
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PMC:7089183Le document en format XML
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uniqKey="Zhang X" first="Xue" last="Zhang">Xue Zhang</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Wang, Ke" sort="Wang, Ke" uniqKey="Wang K" first="Ke" last="Wang">Ke Wang</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Gu, Chunfang" sort="Gu, Chunfang" uniqKey="Gu C" first="Chunfang" last="Gu">Chunfang Gu</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Wu, Kailang" sort="Wu, Kailang" uniqKey="Wu K" first="Kailang" last="Wu">Kailang Wu</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Li, Yan" sort="Li, Yan" uniqKey="Li Y" first="Yan" last="Li">Yan Li</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Zheng, Congyi" sort="Zheng, Congyi" uniqKey="Zheng C" first="Congyi" last="Zheng">Congyi Zheng</name><affiliation><nlm:aff 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id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Zhao, Weiguang" sort="Zhao, Weiguang" uniqKey="Zhao W" first="Weiguang" last="Zhao">Weiguang Zhao</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Zhang, Jianlin" sort="Zhang, Jianlin" uniqKey="Zhang J" first="Jianlin" last="Zhang">Jianlin Zhang</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Zhang, Xue" sort="Zhang, Xue" uniqKey="Zhang X" first="Xue" last="Zhang">Xue Zhang</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Wang, Ke" sort="Wang, Ke" uniqKey="Wang K" first="Ke" last="Wang">Ke Wang</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Gu, Chunfang" sort="Gu, Chunfang" uniqKey="Gu C" first="Chunfang" last="Gu">Chunfang Gu</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Wu, Kailang" sort="Wu, Kailang" uniqKey="Wu K" first="Kailang" last="Wu">Kailang Wu</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Li, Yan" sort="Li, Yan" uniqKey="Li Y" first="Yan" last="Li">Yan Li</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Zheng, Congyi" sort="Zheng, Congyi" uniqKey="Zheng C" first="Congyi" last="Zheng">Congyi Zheng</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Xiao, Gengfu" sort="Xiao, Gengfu" uniqKey="Xiao G" first="Gengfu" last="Xiao">Gengfu Xiao</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Yan, Huimin" sort="Yan, Huimin" uniqKey="Yan H" first="Huimin" last="Yan">Huimin Yan</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Zhang, Jiamin" sort="Zhang, Jiamin" uniqKey="Zhang J" first="Jiamin" last="Zhang">Jiamin Zhang</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Guo, Deyin" sort="Guo, Deyin" uniqKey="Guo D" first="Deyin" last="Guo">Deyin Guo</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Tien, Po" sort="Tien, Po" uniqKey="Tien P" first="Po" last="Tien">Po Tien</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Wu, Jianguo" sort="Wu, Jianguo" uniqKey="Wu J" first="Jianguo" last="Wu">Jianguo Wu</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author></analytic><series><title level="j">Virus Genes</title><idno type="ISSN">0920-8569</idno><idno type="eISSN">1572-994X</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adolescent</term><term>China (epidemiology)</term><term>DNA, Complementary (chemistry)</term><term>DNA, Complementary (isolation & 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séquence</term><term>Chine (épidémiologie)</term><term>Données de séquences moléculaires</term><term>Génome viral</term><term>Humains</term><term>Microscopie électronique</term><term>Mutation</term><term>Mutation faux-sens</term><term>Mutation ponctuelle</term><term>Mâle</term><term>RT-PCR</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Syndrome respiratoire aigu sévère (épidémiologie)</term><term>Virulence (génétique)</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (isolement et purification)</term><term>Virus du SRAS (pathogénicité)</term><term>Épidémiologie moléculaire</term><term>Évolution moléculaire</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Complementary</term></keywords><keywords scheme="MESH" type="geographic" qualifier="epidemiology" 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Reaction</term><term>Sequence Analysis</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ADN complémentaire</term><term>Adolescent</term><term>Analyse de séquence</term><term>Données de séquences moléculaires</term><term>Génome viral</term><term>Humains</term><term>Microscopie électronique</term><term>Mutation</term><term>Mutation faux-sens</term><term>Mutation ponctuelle</term><term>Mâle</term><term>RT-PCR</term><term>Épidémiologie moléculaire</term><term>Évolution moléculaire</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>République populaire de Chine</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Severe acute respiratory syndrome (SARS) caused by SARS-associated coronavirus (SARS-CoV) is a fatal disease. Prevention of future outbreaks is essential and requires understanding pathogenesis and evolution of the virus. We have isolated a SARS-CoV in China and analyzed 47 SARS-CoV genomes with the aims to reveal the evolution trends of the virus and provide insights into understanding pathogenesis and SARS epidemic. Specimen from a SARS patient was inoculated into cell culture. The presence of SARS-CoV was determined by RT-PCR and confirmed by electron microscopy. Virus was isolated followed by the determination of its genome sequences, which were then analyzed by comparing with other 46 SARS-CoV genomes. Genetic mutations with potential implications to pathogenesis and the epidemic were characterized. This viral genome consists of 29,728 nucleotides with overall organization in agreement with that of published isolates. A total of 348 positions were mutated on 47 viral genomes. Among them 22 had mutations in more than three genomes. Hot spots of nucleotide variations and unique trends of mutations were identified on the viral genomes. Mutation rates were different from gene to gene and were correlated well with periodical or geographic characteristics of the epidemic.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Peng, G W" uniqKey="Peng G">G.W. Peng</name></author><author><name sortKey="He, J F" uniqKey="He J">J.F. He</name></author><author><name sortKey="Lin, J Y" uniqKey="Lin J">J.Y. Lin</name></author><author><name sortKey="Zhou, D H" uniqKey="Zhou D">D.H. Zhou</name></author><author><name sortKey="Yu, D W" uniqKey="Yu D">D.W. Yu</name></author><author><name sortKey="Liang, W J" uniqKey="Liang W">W.J. Liang</name></author><author><name sortKey="Li, L H" uniqKey="Li L">L.H. Li</name></author><author><name sortKey="Guo, R N" uniqKey="Guo R">R.N. Guo</name></author><author><name sortKey="Luo, H M" uniqKey="Luo H">H.M. Luo</name></author><author><name sortKey="Xu, R H" uniqKey="Xu R">R.H. Xu</name></author><author><name sortKey="Chin, J" uniqKey="Chin J">J Chin</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Drosten, Christian" uniqKey="Drosten C">Christian Drosten</name></author><author><name sortKey="Gunther, Stephan" uniqKey="Gunther S">Stephan Günther</name></author><author><name sortKey="Preiser, Wolfgang" uniqKey="Preiser W">Wolfgang Preiser</name></author><author><name sortKey="Van Der Werf, Sylvie" uniqKey="Van Der Werf S">Sylvie van der Werf</name></author><author><name sortKey="Brodt, Hans Reinhard" uniqKey="Brodt H">Hans-Reinhard Brodt</name></author><author><name sortKey="Becker, Stephan" uniqKey="Becker S">Stephan Becker</name></author><author><name sortKey="Rabenau, Holger" uniqKey="Rabenau H">Holger Rabenau</name></author><author><name sortKey="Panning, Marcus" uniqKey="Panning M">Marcus Panning</name></author><author><name sortKey="Kolesnikova, Larissa" uniqKey="Kolesnikova L">Larissa Kolesnikova</name></author><author><name sortKey="Fouchier, Ron A M" uniqKey="Fouchier R">Ron A.M. Fouchier</name></author><author><name sortKey="Berger, Annemarie" uniqKey="Berger A">Annemarie Berger</name></author><author><name sortKey="Burguiere, Ana Maria" uniqKey="Burguiere A">Ana-Maria Burguière</name></author><author><name sortKey="Cinatl, Jindrich" uniqKey="Cinatl J">Jindrich Cinatl</name></author><author><name sortKey="Eickmann, Markus" uniqKey="Eickmann M">Markus Eickmann</name></author><author><name sortKey="Escriou, Nicolas" uniqKey="Escriou N">Nicolas Escriou</name></author><author><name sortKey="Grywna, Klaus" uniqKey="Grywna K">Klaus Grywna</name></author><author><name sortKey="Kramme, Stefanie" uniqKey="Kramme S">Stefanie Kramme</name></author><author><name sortKey="Manuguerra, Jean Claude" uniqKey="Manuguerra J">Jean-Claude Manuguerra</name></author><author><name sortKey="Muller, Stefanie" uniqKey="Muller S">Stefanie Müller</name></author><author><name sortKey="Rickerts, Volker" uniqKey="Rickerts V">Volker Rickerts</name></author><author><name sortKey="Sturmer, Martin" uniqKey="Sturmer M">Martin Stürmer</name></author><author><name sortKey="Vieth, Simon" uniqKey="Vieth S">Simon Vieth</name></author><author><name sortKey="Klenk, Hans Dieter" uniqKey="Klenk H">Hans-Dieter Klenk</name></author><author><name sortKey="Osterhaus, Albert D M E" uniqKey="Osterhaus A">Albert D.M.E. 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sortKey="Zhang, Jianlin" sort="Zhang, Jianlin" uniqKey="Zhang J" first="Jianlin" last="Zhang">Jianlin Zhang</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Zhang, Xue" sort="Zhang, Xue" uniqKey="Zhang X" first="Xue" last="Zhang">Xue Zhang</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Wang, Ke" sort="Wang, Ke" uniqKey="Wang K" first="Ke" last="Wang">Ke Wang</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Gu, Chunfang" sort="Gu, Chunfang" uniqKey="Gu C" first="Chunfang" last="Gu">Chunfang Gu</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Wu, Kailang" sort="Wu, Kailang" uniqKey="Wu K" first="Kailang" last="Wu">Kailang Wu</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Li, Yan" sort="Li, Yan" uniqKey="Li Y" first="Yan" last="Li">Yan Li</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Zheng, Congyi" sort="Zheng, Congyi" uniqKey="Zheng C" first="Congyi" last="Zheng">Congyi Zheng</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Xiao, Gengfu" sort="Xiao, Gengfu" uniqKey="Xiao G" first="Gengfu" last="Xiao">Gengfu Xiao</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Yan, Huimin" sort="Yan, Huimin" uniqKey="Yan H" first="Huimin" last="Yan">Huimin Yan</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Zhang, Jiamin" sort="Zhang, Jiamin" uniqKey="Zhang J" first="Jiamin" last="Zhang">Jiamin Zhang</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Guo, Deyin" sort="Guo, Deyin" uniqKey="Guo D" first="Deyin" last="Guo">Deyin Guo</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Tien, Po" sort="Tien, Po" uniqKey="Tien P" first="Po" last="Tien">Po Tien</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Wu, Jianguo" sort="Wu, Jianguo" uniqKey="Wu J" first="Jianguo" last="Wu">Jianguo Wu</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">15744567</idno><idno type="pmc">7089183</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7089183</idno><idno type="RBID">PMC:7089183</idno><idno type="doi">10.1007/s11262-004-4586-9</idno><date when="2005">2005</date><idno type="wicri:Area/Pmc/Corpus">000503</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000503</idno><idno type="wicri:Area/Pmc/Curation">000503</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000503</idno><idno type="wicri:Area/Pmc/Checkpoint">001312</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">001312</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Isolation of Virus from a SARS Patient and Genome-wide Analysis of Genetic Mutations Related to Pathogenesis and Epidemiology from 47 SARS-CoV Isolates</title><author><name sortKey="Zhu, Ying" sort="Zhu, Ying" uniqKey="Zhu Y" first="Ying" last="Zhu">Ying Zhu</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Liu, Mo" sort="Liu, Mo" uniqKey="Liu M" first="Mo" last="Liu">Mo Liu</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Zhao, Weiguang" sort="Zhao, Weiguang" uniqKey="Zhao W" first="Weiguang" last="Zhao">Weiguang Zhao</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Zhang, Jianlin" sort="Zhang, Jianlin" uniqKey="Zhang J" first="Jianlin" last="Zhang">Jianlin Zhang</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Zhang, Xue" sort="Zhang, Xue" uniqKey="Zhang X" first="Xue" last="Zhang">Xue Zhang</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Wang, Ke" sort="Wang, Ke" uniqKey="Wang K" first="Ke" last="Wang">Ke Wang</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Gu, Chunfang" sort="Gu, Chunfang" uniqKey="Gu C" first="Chunfang" last="Gu">Chunfang Gu</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Wu, Kailang" sort="Wu, Kailang" uniqKey="Wu K" first="Kailang" last="Wu">Kailang Wu</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Li, Yan" sort="Li, Yan" uniqKey="Li Y" first="Yan" last="Li">Yan Li</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Zheng, Congyi" sort="Zheng, Congyi" uniqKey="Zheng C" first="Congyi" last="Zheng">Congyi Zheng</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Xiao, Gengfu" sort="Xiao, Gengfu" uniqKey="Xiao G" first="Gengfu" last="Xiao">Gengfu Xiao</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Yan, Huimin" sort="Yan, Huimin" uniqKey="Yan H" first="Huimin" last="Yan">Huimin Yan</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Zhang, Jiamin" sort="Zhang, Jiamin" uniqKey="Zhang J" first="Jiamin" last="Zhang">Jiamin Zhang</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Guo, Deyin" sort="Guo, Deyin" uniqKey="Guo D" first="Deyin" last="Guo">Deyin Guo</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Tien, Po" sort="Tien, Po" uniqKey="Tien P" first="Po" last="Tien">Po Tien</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author><author><name sortKey="Wu, Jianguo" sort="Wu, Jianguo" uniqKey="Wu J" first="Jianguo" last="Wu">Jianguo Wu</name><affiliation><nlm:aff id="Aff1"></nlm:aff></affiliation></author></analytic><series><title level="j">Virus Genes</title><idno type="ISSN">0920-8569</idno><idno type="eISSN">1572-994X</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Severe acute respiratory syndrome (SARS) caused by SARS-associated coronavirus (SARS-CoV) is a fatal disease. Prevention of future outbreaks is essential and requires understanding pathogenesis and evolution of the virus. We have isolated a SARS-CoV in China and analyzed 47 SARS-CoV genomes with the aims to reveal the evolution trends of the virus and provide insights into understanding pathogenesis and SARS epidemic. Specimen from a SARS patient was inoculated into cell culture. The presence of SARS-CoV was determined by RT-PCR and confirmed by electron microscopy. Virus was isolated followed by the determination of its genome sequences, which were then analyzed by comparing with other 46 SARS-CoV genomes. Genetic mutations with potential implications to pathogenesis and the epidemic were characterized. This viral genome consists of 29,728 nucleotides with overall organization in agreement with that of published isolates. A total of 348 positions were mutated on 47 viral genomes. Among them 22 had mutations in more than three genomes. 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Fitch</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chinese, Sars" uniqKey="Chinese S">SARS Chinese</name></author></analytic></biblStruct></listBibl></div1></back></TEI></pmc><pubmed><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Isolation of virus from a SARS patient and genome-wide analysis of genetic mutations related to pathogenesis and epidemiology from 47 SARS-CoV isolates.</title><author><name sortKey="Zhu, Ying" sort="Zhu, Ying" uniqKey="Zhu Y" first="Ying" last="Zhu">Ying Zhu</name><affiliation wicri:level="4"><nlm:affiliation>Key Laboratory of Virology, Ministry of Education College of Life Sciences, Wuhan University, Wuhan 430072, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Virology, Ministry of Education College of Life Sciences, Wuhan University, Wuhan 430072</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName><orgName type="university">Université de Wuhan</orgName><placeName><settlement type="city">Wuhan</settlement><region type="province">Hubei</region></placeName></affiliation></author><author><name sortKey="Liu, Mo" sort="Liu, Mo" uniqKey="Liu M" first="Mo" last="Liu">Mo Liu</name></author><author><name sortKey="Zhao, Weiguang" sort="Zhao, Weiguang" uniqKey="Zhao W" first="Weiguang" last="Zhao">Weiguang Zhao</name></author><author><name sortKey="Zhang, Jianlin" sort="Zhang, Jianlin" uniqKey="Zhang J" first="Jianlin" last="Zhang">Jianlin Zhang</name></author><author><name sortKey="Zhang, Xue" sort="Zhang, Xue" uniqKey="Zhang X" first="Xue" last="Zhang">Xue Zhang</name></author><author><name sortKey="Wang, Ke" sort="Wang, Ke" uniqKey="Wang K" first="Ke" last="Wang">Ke Wang</name></author><author><name sortKey="Gu, Chunfang" sort="Gu, Chunfang" uniqKey="Gu C" first="Chunfang" last="Gu">Chunfang Gu</name></author><author><name sortKey="Wu, Kailang" sort="Wu, Kailang" uniqKey="Wu K" first="Kailang" last="Wu">Kailang Wu</name></author><author><name sortKey="Li, Yan" sort="Li, Yan" uniqKey="Li Y" first="Yan" last="Li">Yan Li</name></author><author><name sortKey="Zheng, Congyi" sort="Zheng, Congyi" uniqKey="Zheng C" first="Congyi" last="Zheng">Congyi Zheng</name></author><author><name sortKey="Xiao, Gengfu" sort="Xiao, Gengfu" uniqKey="Xiao G" first="Gengfu" last="Xiao">Gengfu Xiao</name></author><author><name sortKey="Yan, Huimin" sort="Yan, Huimin" uniqKey="Yan H" first="Huimin" last="Yan">Huimin Yan</name></author><author><name sortKey="Zhang, Jiamin" sort="Zhang, Jiamin" uniqKey="Zhang J" first="Jiamin" last="Zhang">Jiamin Zhang</name></author><author><name sortKey="Guo, Deyin" sort="Guo, Deyin" uniqKey="Guo D" first="Deyin" last="Guo">Deyin Guo</name></author><author><name sortKey="Tien, Po" sort="Tien, Po" uniqKey="Tien P" first="Po" last="Tien">Po Tien</name></author><author><name sortKey="Wu, Jianguo" sort="Wu, Jianguo" uniqKey="Wu J" first="Jianguo" last="Wu">Jianguo Wu</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2005">2005</date><idno type="RBID">pubmed:15744567</idno><idno type="pmid">15744567</idno><idno type="doi">10.1007/s11262-004-4586-9</idno><idno type="wicri:Area/PubMed/Corpus">002864</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002864</idno><idno type="wicri:Area/PubMed/Curation">002864</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002864</idno><idno type="wicri:Area/PubMed/Checkpoint">002640</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002640</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Isolation of virus from a SARS patient and genome-wide analysis of genetic mutations related to pathogenesis and epidemiology from 47 SARS-CoV isolates.</title><author><name sortKey="Zhu, Ying" sort="Zhu, Ying" uniqKey="Zhu Y" first="Ying" last="Zhu">Ying Zhu</name><affiliation wicri:level="4"><nlm:affiliation>Key Laboratory of Virology, Ministry of Education College of Life Sciences, Wuhan University, Wuhan 430072, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Key Laboratory of Virology, Ministry of Education College of Life Sciences, Wuhan University, Wuhan 430072</wicri:regionArea><placeName><settlement type="city">Wuhan</settlement><region type="région">Hubei</region></placeName><orgName type="university">Université de Wuhan</orgName><placeName><settlement type="city">Wuhan</settlement><region type="province">Hubei</region></placeName></affiliation></author><author><name sortKey="Liu, Mo" sort="Liu, Mo" uniqKey="Liu M" first="Mo" last="Liu">Mo Liu</name></author><author><name sortKey="Zhao, Weiguang" sort="Zhao, Weiguang" uniqKey="Zhao W" first="Weiguang" last="Zhao">Weiguang Zhao</name></author><author><name sortKey="Zhang, Jianlin" sort="Zhang, Jianlin" uniqKey="Zhang J" first="Jianlin" last="Zhang">Jianlin Zhang</name></author><author><name sortKey="Zhang, Xue" sort="Zhang, Xue" uniqKey="Zhang X" first="Xue" last="Zhang">Xue Zhang</name></author><author><name sortKey="Wang, Ke" sort="Wang, Ke" uniqKey="Wang K" first="Ke" last="Wang">Ke Wang</name></author><author><name sortKey="Gu, Chunfang" sort="Gu, Chunfang" uniqKey="Gu C" first="Chunfang" last="Gu">Chunfang Gu</name></author><author><name sortKey="Wu, Kailang" sort="Wu, Kailang" uniqKey="Wu K" first="Kailang" last="Wu">Kailang Wu</name></author><author><name sortKey="Li, Yan" sort="Li, Yan" uniqKey="Li Y" first="Yan" last="Li">Yan Li</name></author><author><name sortKey="Zheng, Congyi" sort="Zheng, Congyi" uniqKey="Zheng C" first="Congyi" last="Zheng">Congyi Zheng</name></author><author><name sortKey="Xiao, Gengfu" sort="Xiao, Gengfu" uniqKey="Xiao G" first="Gengfu" last="Xiao">Gengfu Xiao</name></author><author><name sortKey="Yan, Huimin" sort="Yan, Huimin" uniqKey="Yan H" first="Huimin" last="Yan">Huimin Yan</name></author><author><name sortKey="Zhang, Jiamin" sort="Zhang, Jiamin" uniqKey="Zhang J" first="Jiamin" last="Zhang">Jiamin Zhang</name></author><author><name sortKey="Guo, Deyin" sort="Guo, Deyin" uniqKey="Guo D" first="Deyin" last="Guo">Deyin Guo</name></author><author><name sortKey="Tien, Po" sort="Tien, Po" uniqKey="Tien P" first="Po" last="Tien">Po Tien</name></author><author><name sortKey="Wu, Jianguo" sort="Wu, Jianguo" uniqKey="Wu J" first="Jianguo" last="Wu">Jianguo Wu</name></author></analytic><series><title level="j">Virus genes</title><idno type="ISSN">0920-8569</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adolescent</term><term>China (epidemiology)</term><term>DNA, Complementary (chemistry)</term><term>DNA, Complementary (isolation & purification)</term><term>Evolution, Molecular</term><term>Genome, Viral</term><term>Humans</term><term>Male</term><term>Microscopy, Electron</term><term>Molecular Epidemiology</term><term>Molecular Sequence Data</term><term>Mutation</term><term>Mutation, Missense</term><term>Point Mutation</term><term>RNA, Viral (analysis)</term><term>RNA, Viral (genetics)</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>SARS Virus (genetics)</term><term>SARS Virus (isolation & purification)</term><term>SARS Virus (pathogenicity)</term><term>Sequence Analysis</term><term>Severe Acute Respiratory Syndrome (epidemiology)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>Virulence (genetics)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire ()</term><term>ADN complémentaire (isolement et purification)</term><term>ARN viral (analyse)</term><term>ARN viral (génétique)</term><term>Adolescent</term><term>Analyse de séquence</term><term>Chine (épidémiologie)</term><term>Données de séquences moléculaires</term><term>Génome viral</term><term>Humains</term><term>Microscopie électronique</term><term>Mutation</term><term>Mutation faux-sens</term><term>Mutation ponctuelle</term><term>Mâle</term><term>RT-PCR</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Syndrome respiratoire aigu sévère (épidémiologie)</term><term>Virulence (génétique)</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (isolement et purification)</term><term>Virus du SRAS (pathogénicité)</term><term>Épidémiologie moléculaire</term><term>Évolution moléculaire</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>DNA, Complementary</term></keywords><keywords scheme="MESH" type="geographic" qualifier="epidemiology" xml:lang="en"><term>China</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>DNA, Complementary</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ARN viral</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term><term>Virulence</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN viral</term><term>Virulence</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>ADN complémentaire</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Chine</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adolescent</term><term>Evolution, Molecular</term><term>Genome, Viral</term><term>Humans</term><term>Male</term><term>Microscopy, Electron</term><term>Molecular Epidemiology</term><term>Molecular Sequence Data</term><term>Mutation</term><term>Mutation, Missense</term><term>Point Mutation</term><term>Reverse Transcriptase Polymerase Chain Reaction</term><term>Sequence Analysis</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>ADN complémentaire</term><term>Adolescent</term><term>Analyse de séquence</term><term>Données de séquences moléculaires</term><term>Génome viral</term><term>Humains</term><term>Microscopie électronique</term><term>Mutation</term><term>Mutation faux-sens</term><term>Mutation ponctuelle</term><term>Mâle</term><term>RT-PCR</term><term>Épidémiologie moléculaire</term><term>Évolution moléculaire</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>République populaire de Chine</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) caused by SARS-associated coronavirus (SARS-CoV) is a fatal disease. Prevention of future outbreaks is essential and requires understanding pathogenesis and evolution of the virus. We have isolated a SARS-CoV in China and analyzed 47 SARS-CoV genomes with the aims to reveal the evolution trends of the virus and provide insights into understanding pathogenesis and SARS epidemic. Specimen from a SARS patient was inoculated into cell culture. The presence of SARS-CoV was determined by RT-PCR and confirmed by electron microscopy. Virus was isolated followed by the determination of its genome sequences, which were then analyzed by comparing with other 46 SARS-CoV genomes. Genetic mutations with potential implications to pathogenesis and the epidemic were characterized. This viral genome consists of 29,728 nucleotides with overall organization in agreement with that of published isolates. A total of 348 positions were mutated on 47 viral genomes. Among them 22 had mutations in more than three genomes. Hot spots of nucleotide variations and unique trends of mutations were identified on the viral genomes. Mutation rates were different from gene to gene and were correlated well with periodical or geographic characteristics of the epidemic.</div></front></TEI></pubmed></double></record>Pour manipuler ce document sous Unix (Dilib)
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