Crystallization and preliminary crystallographic analysis of the heptad-repeat complex of SARS coronavirus spike protein.
Identifieur interne : 000C52 ( Ncbi/Merge ); précédent : 000C51; suivant : 000C53Crystallization and preliminary crystallographic analysis of the heptad-repeat complex of SARS coronavirus spike protein.
Auteurs : Yanhui Xu [République populaire de Chine] ; Nan Su ; Lan Qin ; Zhihong Bai ; George F. Gao ; Zihe RaoSource :
- Acta crystallographica. Section D, Biological crystallography [ 0907-4449 ] ; 2004.
Descripteurs français
- KwdFr :
- Concentration en ions d'hydrogène, Conformation des protéines, Cristallisation, Cristallographie aux rayons X, Diffraction des rayons X, Fusion membranaire, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (), Génome viral, Modèles statistiques, Protéines de fusion virale (), Protéines de l'enveloppe virale (), Structure tertiaire des protéines, Température.
- MESH :
- Concentration en ions d'hydrogène, Conformation des protéines, Cristallisation, Cristallographie aux rayons X, Diffraction des rayons X, Fusion membranaire, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires, Génome viral, Modèles statistiques, Protéines de fusion virale, Protéines de l'enveloppe virale, Structure tertiaire des protéines, Température.
English descriptors
- KwdEn :
- Crystallization, Crystallography, X-Ray, Genome, Viral, Hydrogen-Ion Concentration, Membrane Fusion, Membrane Glycoproteins (chemistry), Models, Statistical, Protein Conformation, Protein Structure, Tertiary, Spike Glycoprotein, Coronavirus, Temperature, Viral Envelope Proteins (chemistry), Viral Fusion Proteins (chemistry), X-Ray Diffraction.
- MESH :
- chemical , chemistry : Membrane Glycoproteins, Viral Envelope Proteins, Viral Fusion Proteins.
- Crystallization, Crystallography, X-Ray, Genome, Viral, Hydrogen-Ion Concentration, Membrane Fusion, Models, Statistical, Protein Conformation, Protein Structure, Tertiary, Spike Glycoprotein, Coronavirus, Temperature, X-Ray Diffraction.
Abstract
The aetiological agent of an emergent outbreak of atypical pneumonia, severe acute respiratory syndrome (SARS), is a positive-stranded RNA virus (SARS-CoV) belonging to the Coronaviridae family with a genome that differs substantially from those of other known coronaviruses. Highly conserved heptad-repeat (HR1 and HR2) regions in class I viral fusion proteins, including spike protein from SARS coronavirus, interact with each other to form a six-helix bundle, which is called a fusion core. The crystal structure of the fusion core is expected to greatly facilitate drug design. Crystals of the fusion core of SARS-CoV spike protein have been grown at 291 K using PEG 4000 as precipitant. The diffraction pattern of the crystal extends to 2.8 A resolution at 100 K in-house. The crystals have unit-cell parameters a = 121.2, b = 66.3, c = 70.0 A, alpha = gamma = 90, beta = 107.4 degrees and belong to space group C2. Assuming the presence of six molecules per asymmetric unit, the solvent content is estimated to be about 28%.
DOI: 10.1107/S0907444904027258
PubMed: 15583393
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 002A12
- to stream PubMed, to step Curation: 002A12
- to stream PubMed, to step Checkpoint: 002D61
Links to Exploration step
pubmed:15583393Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Crystallization and preliminary crystallographic analysis of the heptad-repeat complex of SARS coronavirus spike protein.</title>
<author><name sortKey="Xu, Yanhui" sort="Xu, Yanhui" uniqKey="Xu Y" first="Yanhui" last="Xu">Yanhui Xu</name>
<affiliation wicri:level="1"><nlm:affiliation>Laboratory of Structural Biology, Tsinghua University, Beijing 100084, People's Republic of China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Laboratory of Structural Biology, Tsinghua University, Beijing 100084</wicri:regionArea>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Su, Nan" sort="Su, Nan" uniqKey="Su N" first="Nan" last="Su">Nan Su</name>
</author>
<author><name sortKey="Qin, Lan" sort="Qin, Lan" uniqKey="Qin L" first="Lan" last="Qin">Lan Qin</name>
</author>
<author><name sortKey="Bai, Zhihong" sort="Bai, Zhihong" uniqKey="Bai Z" first="Zhihong" last="Bai">Zhihong Bai</name>
</author>
<author><name sortKey="Gao, George F" sort="Gao, George F" uniqKey="Gao G" first="George F" last="Gao">George F. Gao</name>
</author>
<author><name sortKey="Rao, Zihe" sort="Rao, Zihe" uniqKey="Rao Z" first="Zihe" last="Rao">Zihe Rao</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2004">2004</date>
<idno type="RBID">pubmed:15583393</idno>
<idno type="pmid">15583393</idno>
<idno type="doi">10.1107/S0907444904027258</idno>
<idno type="wicri:Area/PubMed/Corpus">002A12</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002A12</idno>
<idno type="wicri:Area/PubMed/Curation">002A12</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002A12</idno>
<idno type="wicri:Area/PubMed/Checkpoint">002D61</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002D61</idno>
<idno type="wicri:Area/Ncbi/Merge">000C52</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">Crystallization and preliminary crystallographic analysis of the heptad-repeat complex of SARS coronavirus spike protein.</title>
<author><name sortKey="Xu, Yanhui" sort="Xu, Yanhui" uniqKey="Xu Y" first="Yanhui" last="Xu">Yanhui Xu</name>
<affiliation wicri:level="1"><nlm:affiliation>Laboratory of Structural Biology, Tsinghua University, Beijing 100084, People's Republic of China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Laboratory of Structural Biology, Tsinghua University, Beijing 100084</wicri:regionArea>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Su, Nan" sort="Su, Nan" uniqKey="Su N" first="Nan" last="Su">Nan Su</name>
</author>
<author><name sortKey="Qin, Lan" sort="Qin, Lan" uniqKey="Qin L" first="Lan" last="Qin">Lan Qin</name>
</author>
<author><name sortKey="Bai, Zhihong" sort="Bai, Zhihong" uniqKey="Bai Z" first="Zhihong" last="Bai">Zhihong Bai</name>
</author>
<author><name sortKey="Gao, George F" sort="Gao, George F" uniqKey="Gao G" first="George F" last="Gao">George F. Gao</name>
</author>
<author><name sortKey="Rao, Zihe" sort="Rao, Zihe" uniqKey="Rao Z" first="Zihe" last="Rao">Zihe Rao</name>
</author>
</analytic>
<series><title level="j">Acta crystallographica. Section D, Biological crystallography</title>
<idno type="ISSN">0907-4449</idno>
<imprint><date when="2004" type="published">2004</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Crystallization</term>
<term>Crystallography, X-Ray</term>
<term>Genome, Viral</term>
<term>Hydrogen-Ion Concentration</term>
<term>Membrane Fusion</term>
<term>Membrane Glycoproteins (chemistry)</term>
<term>Models, Statistical</term>
<term>Protein Conformation</term>
<term>Protein Structure, Tertiary</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Temperature</term>
<term>Viral Envelope Proteins (chemistry)</term>
<term>Viral Fusion Proteins (chemistry)</term>
<term>X-Ray Diffraction</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Concentration en ions d'hydrogène</term>
<term>Conformation des protéines</term>
<term>Cristallisation</term>
<term>Cristallographie aux rayons X</term>
<term>Diffraction des rayons X</term>
<term>Fusion membranaire</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires ()</term>
<term>Génome viral</term>
<term>Modèles statistiques</term>
<term>Protéines de fusion virale ()</term>
<term>Protéines de l'enveloppe virale ()</term>
<term>Structure tertiaire des protéines</term>
<term>Température</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Membrane Glycoproteins</term>
<term>Viral Envelope Proteins</term>
<term>Viral Fusion Proteins</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Crystallization</term>
<term>Crystallography, X-Ray</term>
<term>Genome, Viral</term>
<term>Hydrogen-Ion Concentration</term>
<term>Membrane Fusion</term>
<term>Models, Statistical</term>
<term>Protein Conformation</term>
<term>Protein Structure, Tertiary</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Temperature</term>
<term>X-Ray Diffraction</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Concentration en ions d'hydrogène</term>
<term>Conformation des protéines</term>
<term>Cristallisation</term>
<term>Cristallographie aux rayons X</term>
<term>Diffraction des rayons X</term>
<term>Fusion membranaire</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires</term>
<term>Génome viral</term>
<term>Modèles statistiques</term>
<term>Protéines de fusion virale</term>
<term>Protéines de l'enveloppe virale</term>
<term>Structure tertiaire des protéines</term>
<term>Température</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The aetiological agent of an emergent outbreak of atypical pneumonia, severe acute respiratory syndrome (SARS), is a positive-stranded RNA virus (SARS-CoV) belonging to the Coronaviridae family with a genome that differs substantially from those of other known coronaviruses. Highly conserved heptad-repeat (HR1 and HR2) regions in class I viral fusion proteins, including spike protein from SARS coronavirus, interact with each other to form a six-helix bundle, which is called a fusion core. The crystal structure of the fusion core is expected to greatly facilitate drug design. Crystals of the fusion core of SARS-CoV spike protein have been grown at 291 K using PEG 4000 as precipitant. The diffraction pattern of the crystal extends to 2.8 A resolution at 100 K in-house. The crystals have unit-cell parameters a = 121.2, b = 66.3, c = 70.0 A, alpha = gamma = 90, beta = 107.4 degrees and belong to space group C2. Assuming the presence of six molecules per asymmetric unit, the solvent content is estimated to be about 28%.</div>
</front>
</TEI>
<pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15583393</PMID>
<DateCompleted><Year>2005</Year>
<Month>05</Month>
<Day>24</Day>
</DateCompleted>
<DateRevised><Year>2020</Year>
<Month>04</Month>
<Day>18</Day>
</DateRevised>
<Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0907-4449</ISSN>
<JournalIssue CitedMedium="Print"><Volume>60</Volume>
<Issue>Pt 12 Pt 2</Issue>
<PubDate><Year>2004</Year>
<Month>Dec</Month>
</PubDate>
</JournalIssue>
<Title>Acta crystallographica. Section D, Biological crystallography</Title>
<ISOAbbreviation>Acta Crystallogr. D Biol. Crystallogr.</ISOAbbreviation>
</Journal>
<ArticleTitle>Crystallization and preliminary crystallographic analysis of the heptad-repeat complex of SARS coronavirus spike protein.</ArticleTitle>
<Pagination><MedlinePgn>2377-9</MedlinePgn>
</Pagination>
<Abstract><AbstractText>The aetiological agent of an emergent outbreak of atypical pneumonia, severe acute respiratory syndrome (SARS), is a positive-stranded RNA virus (SARS-CoV) belonging to the Coronaviridae family with a genome that differs substantially from those of other known coronaviruses. Highly conserved heptad-repeat (HR1 and HR2) regions in class I viral fusion proteins, including spike protein from SARS coronavirus, interact with each other to form a six-helix bundle, which is called a fusion core. The crystal structure of the fusion core is expected to greatly facilitate drug design. Crystals of the fusion core of SARS-CoV spike protein have been grown at 291 K using PEG 4000 as precipitant. The diffraction pattern of the crystal extends to 2.8 A resolution at 100 K in-house. The crystals have unit-cell parameters a = 121.2, b = 66.3, c = 70.0 A, alpha = gamma = 90, beta = 107.4 degrees and belong to space group C2. Assuming the presence of six molecules per asymmetric unit, the solvent content is estimated to be about 28%.</AbstractText>
</Abstract>
<AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Xu</LastName>
<ForeName>Yanhui</ForeName>
<Initials>Y</Initials>
<AffiliationInfo><Affiliation>Laboratory of Structural Biology, Tsinghua University, Beijing 100084, People's Republic of China.</Affiliation>
</AffiliationInfo>
</Author>
<Author ValidYN="Y"><LastName>Su</LastName>
<ForeName>Nan</ForeName>
<Initials>N</Initials>
</Author>
<Author ValidYN="Y"><LastName>Qin</LastName>
<ForeName>Lan</ForeName>
<Initials>L</Initials>
</Author>
<Author ValidYN="Y"><LastName>Bai</LastName>
<ForeName>Zhihong</ForeName>
<Initials>Z</Initials>
</Author>
<Author ValidYN="Y"><LastName>Gao</LastName>
<ForeName>George F</ForeName>
<Initials>GF</Initials>
</Author>
<Author ValidYN="Y"><LastName>Rao</LastName>
<ForeName>Zihe</ForeName>
<Initials>Z</Initials>
</Author>
</AuthorList>
<Language>eng</Language>
<PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType>
<PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType>
</PublicationTypeList>
<ArticleDate DateType="Electronic"><Year>2004</Year>
<Month>11</Month>
<Day>26</Day>
</ArticleDate>
</Article>
<MedlineJournalInfo><Country>United States</Country>
<MedlineTA>Acta Crystallogr D Biol Crystallogr</MedlineTA>
<NlmUniqueID>9305878</NlmUniqueID>
<ISSNLinking>0907-4449</ISSNLinking>
</MedlineJournalInfo>
<ChemicalList><Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D008562">Membrane Glycoproteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D064370">Spike Glycoprotein, Coronavirus</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D014759">Viral Envelope Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="D014760">Viral Fusion Proteins</NameOfSubstance>
</Chemical>
<Chemical><RegistryNumber>0</RegistryNumber>
<NameOfSubstance UI="C578557">spike glycoprotein, SARS-CoV</NameOfSubstance>
</Chemical>
</ChemicalList>
<CitationSubset>IM</CitationSubset>
<MeshHeadingList><MeshHeading><DescriptorName UI="D003460" MajorTopicYN="N">Crystallization</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D018360" MajorTopicYN="N">Crystallography, X-Ray</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D016679" MajorTopicYN="N">Genome, Viral</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D006863" MajorTopicYN="N">Hydrogen-Ion Concentration</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008561" MajorTopicYN="N">Membrane Fusion</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D008562" MajorTopicYN="N">Membrane Glycoproteins</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D015233" MajorTopicYN="N">Models, Statistical</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D011487" MajorTopicYN="N">Protein Conformation</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D017434" MajorTopicYN="N">Protein Structure, Tertiary</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D064370" MajorTopicYN="N">Spike Glycoprotein, Coronavirus</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D013696" MajorTopicYN="N">Temperature</DescriptorName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014759" MajorTopicYN="N">Viral Envelope Proteins</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014760" MajorTopicYN="N">Viral Fusion Proteins</DescriptorName>
<QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName>
</MeshHeading>
<MeshHeading><DescriptorName UI="D014961" MajorTopicYN="N">X-Ray Diffraction</DescriptorName>
</MeshHeading>
</MeshHeadingList>
</MedlineCitation>
<PubmedData><History><PubMedPubDate PubStatus="received"><Year>2004</Year>
<Month>09</Month>
<Day>02</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="accepted"><Year>2004</Year>
<Month>10</Month>
<Day>25</Day>
</PubMedPubDate>
<PubMedPubDate PubStatus="pubmed"><Year>2004</Year>
<Month>12</Month>
<Day>8</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="medline"><Year>2005</Year>
<Month>5</Month>
<Day>25</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
<PubMedPubDate PubStatus="entrez"><Year>2004</Year>
<Month>12</Month>
<Day>8</Day>
<Hour>9</Hour>
<Minute>0</Minute>
</PubMedPubDate>
</History>
<PublicationStatus>ppublish</PublicationStatus>
<ArticleIdList><ArticleId IdType="pubmed">15583393</ArticleId>
<ArticleId IdType="pii">S0907444904027258</ArticleId>
<ArticleId IdType="doi">10.1107/S0907444904027258</ArticleId>
<ArticleId IdType="pmc">PMC7161635</ArticleId>
</ArticleIdList>
</PubmedData>
</pubmed>
<affiliations><list><country><li>République populaire de Chine</li>
</country>
<settlement><li>Pékin</li>
</settlement>
</list>
<tree><noCountry><name sortKey="Bai, Zhihong" sort="Bai, Zhihong" uniqKey="Bai Z" first="Zhihong" last="Bai">Zhihong Bai</name>
<name sortKey="Gao, George F" sort="Gao, George F" uniqKey="Gao G" first="George F" last="Gao">George F. Gao</name>
<name sortKey="Qin, Lan" sort="Qin, Lan" uniqKey="Qin L" first="Lan" last="Qin">Lan Qin</name>
<name sortKey="Rao, Zihe" sort="Rao, Zihe" uniqKey="Rao Z" first="Zihe" last="Rao">Zihe Rao</name>
<name sortKey="Su, Nan" sort="Su, Nan" uniqKey="Su N" first="Nan" last="Su">Nan Su</name>
</noCountry>
<country name="République populaire de Chine"><noRegion><name sortKey="Xu, Yanhui" sort="Xu, Yanhui" uniqKey="Xu Y" first="Yanhui" last="Xu">Yanhui Xu</name>
</noRegion>
</country>
</tree>
</affiliations>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Ncbi/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000C52 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd -nk 000C52 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= SrasV1 |flux= Ncbi |étape= Merge |type= RBID |clé= pubmed:15583393 |texte= Crystallization and preliminary crystallographic analysis of the heptad-repeat complex of SARS coronavirus spike protein. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/RBID.i -Sk "pubmed:15583393" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd \ | NlmPubMed2Wicri -a SrasV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |