Quantitation of severe acute respiratory syndrome coronavirus genome by real-time polymerase chain reaction assay using minor groove binder DNA probe technology.
Identifieur interne : 000B39 ( Ncbi/Merge ); précédent : 000B38; suivant : 000B40Quantitation of severe acute respiratory syndrome coronavirus genome by real-time polymerase chain reaction assay using minor groove binder DNA probe technology.
Auteurs : Hsi-Hsun Lin [République populaire de Chine] ; Shiow-Jen Wang ; Yung-Ching Liu ; Susan Shin-Jung Lee ; Chun-Kai Hwang ; Yao-Shen Chen ; Shue-Ren Wann ; Yi-Li ShihSource :
- Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi [ 1684-1182 ] ; 2004.
Descripteurs français
- KwdFr :
- ADN complémentaire (génétique), ADN viral (génétique), Flambées de maladies, Génome viral, Humains, RT-PCR (), Sensibilité et spécificité, Sondes d'ADN (génétique), Syndrome respiratoire aigu sévère (diagnostic), Syndrome respiratoire aigu sévère (virologie), Syndrome respiratoire aigu sévère (épidémiologie), Séquence nucléotidique, Taïwan (épidémiologie), Techniques de sonde moléculaire, Virus du SRAS (génétique), Virus du SRAS (isolement et purification).
- MESH :
- diagnostic : Syndrome respiratoire aigu sévère.
- génétique : ADN complémentaire, ADN viral, Sondes d'ADN, Virus du SRAS.
- isolement et purification : Virus du SRAS.
- virologie : Syndrome respiratoire aigu sévère.
- épidémiologie : Syndrome respiratoire aigu sévère, Taïwan.
- Flambées de maladies, Génome viral, Humains, RT-PCR, Sensibilité et spécificité, Séquence nucléotidique, Techniques de sonde moléculaire.
- Wicri :
- geographic : Taïwan.
English descriptors
- KwdEn :
- Base Sequence, DNA Probes (genetics), DNA, Complementary (genetics), DNA, Viral (genetics), Disease Outbreaks, Genome, Viral, Humans, Molecular Probe Techniques, Reverse Transcriptase Polymerase Chain Reaction (methods), Reverse Transcriptase Polymerase Chain Reaction (statistics & numerical data), SARS Virus (genetics), SARS Virus (isolation & purification), Sensitivity and Specificity, Severe Acute Respiratory Syndrome (diagnosis), Severe Acute Respiratory Syndrome (epidemiology), Severe Acute Respiratory Syndrome (virology), Taiwan (epidemiology).
- MESH :
- chemical , genetics : DNA Probes, DNA, Complementary, DNA, Viral.
- geographic , epidemiology : Taiwan.
- diagnosis : Severe Acute Respiratory Syndrome.
- epidemiology : Severe Acute Respiratory Syndrome.
- genetics : SARS Virus.
- isolation & purification : SARS Virus.
- methods : Reverse Transcriptase Polymerase Chain Reaction.
- statistics & numerical data : Reverse Transcriptase Polymerase Chain Reaction.
- virology : Severe Acute Respiratory Syndrome.
- Base Sequence, Disease Outbreaks, Genome, Viral, Humans, Molecular Probe Techniques, Sensitivity and Specificity.
Abstract
The ability to rapidly recognize severe acute respiratory syndrome coronavirus (SARS-CoV) as a cause of infections is critical to quickly limiting further spread of the disease. A rapid, sensitive, and specific laboratory diagnostic test is needed to confirm outbreaks of SARS-CoV infection and distinguish it from other diseases that can cause similar clinical symptoms. An improved TaqMan technology using minor groove binder (MGB) probes was used to detect and quantify SARS-CoV in suspected patients. SARS-CoV primers and probe were designed based on the open reading frame 1b sequence, which encodes coronavirus replicase protein. A linear standard curve with R2 > 0.99 was obtained, and the threshold sensitivity was 10 genome equivalents per reaction. Interassay coefficients of variation were 1.73 to 2.72%, indicating good reproducibility of this method. A total of 228 specimens from 151 suspected patients were quantified by this method, 13 specimens from 6 patients were positive with viral load range from 362 to 36,240,000 genome equivalents/mL. In conclusion, the high sensitivity and reproducibility of the real-time polymerase chain reaction SARS-CoV RNA quantitation using MGB probe allowed the screening of large numbers of clinical samples.
PubMed: 15497005
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Taiwan, ROC.</nlm:affiliation><country xml:lang="fr" wicri:curation="lc">République populaire de Chine</country><wicri:regionArea>Section of Infectious Diseases, Department of Medicine, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan</wicri:regionArea><wicri:noRegion>Taiwan</wicri:noRegion></affiliation></author><author><name sortKey="Wang, Shiow Jen" sort="Wang, Shiow Jen" uniqKey="Wang S" first="Shiow-Jen" last="Wang">Shiow-Jen Wang</name></author><author><name sortKey="Liu, Yung Ching" sort="Liu, Yung Ching" uniqKey="Liu Y" first="Yung-Ching" last="Liu">Yung-Ching Liu</name></author><author><name sortKey="Lee, Susan Shin Jung" sort="Lee, Susan Shin Jung" uniqKey="Lee S" first="Susan Shin-Jung" last="Lee">Susan Shin-Jung Lee</name></author><author><name sortKey="Hwang, Chun Kai" sort="Hwang, Chun Kai" uniqKey="Hwang C" first="Chun-Kai" last="Hwang">Chun-Kai Hwang</name></author><author><name sortKey="Chen, Yao Shen" sort="Chen, Yao Shen" uniqKey="Chen Y" first="Yao-Shen" 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Reaction (statistics & numerical data)</term><term>SARS Virus (genetics)</term><term>SARS Virus (isolation & purification)</term><term>Sensitivity and Specificity</term><term>Severe Acute Respiratory Syndrome (diagnosis)</term><term>Severe Acute Respiratory Syndrome (epidemiology)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>Taiwan (epidemiology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN complémentaire (génétique)</term><term>ADN viral (génétique)</term><term>Flambées de maladies</term><term>Génome viral</term><term>Humains</term><term>RT-PCR ()</term><term>Sensibilité et spécificité</term><term>Sondes d'ADN (génétique)</term><term>Syndrome respiratoire aigu sévère (diagnostic)</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Syndrome respiratoire aigu sévère (épidémiologie)</term><term>Séquence nucléotidique</term><term>Taïwan (épidémiologie)</term><term>Techniques de sonde moléculaire</term><term>Virus du SRAS 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Viral</term><term>Humans</term><term>Molecular Probe Techniques</term><term>Sensitivity and Specificity</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Flambées de maladies</term><term>Génome viral</term><term>Humains</term><term>RT-PCR</term><term>Sensibilité et spécificité</term><term>Séquence nucléotidique</term><term>Techniques de sonde moléculaire</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>Taïwan</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The ability to rapidly recognize severe acute respiratory syndrome coronavirus (SARS-CoV) as a cause of infections is critical to quickly limiting further spread of the disease. A rapid, sensitive, and specific laboratory diagnostic test is needed to confirm outbreaks of SARS-CoV infection and distinguish it from other diseases that can cause similar clinical symptoms. An improved TaqMan technology using minor groove binder (MGB) probes was used to detect and quantify SARS-CoV in suspected patients. SARS-CoV primers and probe were designed based on the open reading frame 1b sequence, which encodes coronavirus replicase protein. A linear standard curve with R2 > 0.99 was obtained, and the threshold sensitivity was 10 genome equivalents per reaction. Interassay coefficients of variation were 1.73 to 2.72%, indicating good reproducibility of this method. A total of 228 specimens from 151 suspected patients were quantified by this method, 13 specimens from 6 patients were positive with viral load range from 362 to 36,240,000 genome equivalents/mL. In conclusion, the high sensitivity and reproducibility of the real-time polymerase chain reaction SARS-CoV RNA quantitation using MGB probe allowed the screening of large numbers of clinical samples.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15497005</PMID><DateCompleted><Year>2004</Year><Month>12</Month><Day>10</Day></DateCompleted><DateRevised><Year>2019</Year><Month>12</Month><Day>10</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1684-1182</ISSN><JournalIssue CitedMedium="Print"><Volume>37</Volume><Issue>5</Issue><PubDate><Year>2004</Year><Month>Oct</Month></PubDate></JournalIssue><Title>Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi</Title><ISOAbbreviation>J Microbiol Immunol Infect</ISOAbbreviation></Journal><ArticleTitle>Quantitation of severe acute respiratory syndrome coronavirus genome by real-time polymerase chain reaction assay using minor groove binder DNA probe technology.</ArticleTitle><Pagination><MedlinePgn>258-65</MedlinePgn></Pagination><Abstract><AbstractText>The ability to rapidly recognize severe acute respiratory syndrome coronavirus (SARS-CoV) as a cause of infections is critical to quickly limiting further spread of the disease. A rapid, sensitive, and specific laboratory diagnostic test is needed to confirm outbreaks of SARS-CoV infection and distinguish it from other diseases that can cause similar clinical symptoms. An improved TaqMan technology using minor groove binder (MGB) probes was used to detect and quantify SARS-CoV in suspected patients. SARS-CoV primers and probe were designed based on the open reading frame 1b sequence, which encodes coronavirus replicase protein. A linear standard curve with R2 > 0.99 was obtained, and the threshold sensitivity was 10 genome equivalents per reaction. Interassay coefficients of variation were 1.73 to 2.72%, indicating good reproducibility of this method. A total of 228 specimens from 151 suspected patients were quantified by this method, 13 specimens from 6 patients were positive with viral load range from 362 to 36,240,000 genome equivalents/mL. In conclusion, the high sensitivity and reproducibility of the real-time polymerase chain reaction SARS-CoV RNA quantitation using MGB probe allowed the screening of large numbers of clinical samples.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Lin</LastName><ForeName>Hsi-Hsun</ForeName><Initials>HH</Initials><AffiliationInfo><Affiliation>Section of Infectious Diseases, Department of Medicine, Kaohsiung Veterans General Hospital, Kaohsiung, Taiwan, ROC.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Shiow-Jen</ForeName><Initials>SJ</Initials></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Yung-Ching</ForeName><Initials>YC</Initials></Author><Author ValidYN="Y"><LastName>Lee</LastName><ForeName>Susan Shin-Jung</ForeName><Initials>SS</Initials></Author><Author ValidYN="Y"><LastName>Hwang</LastName><ForeName>Chun-Kai</ForeName><Initials>CK</Initials></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Yao-Shen</ForeName><Initials>YS</Initials></Author><Author ValidYN="Y"><LastName>Wann</LastName><ForeName>Shue-Ren</ForeName><Initials>SR</Initials></Author><Author ValidYN="Y"><LastName>Shih</LastName><ForeName>Yi-Li</ForeName><Initials>YL</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D023362">Evaluation Study</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>England</Country><MedlineTA>J Microbiol Immunol Infect</MedlineTA><NlmUniqueID>100956211</NlmUniqueID><ISSNLinking>1684-1182</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D015342">DNA Probes</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D018076">DNA, 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MajorTopicYN="Y">Genome, Viral</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D015336" MajorTopicYN="N">Molecular Probe Techniques</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D020133" MajorTopicYN="N">Reverse Transcriptase Polymerase Chain Reaction</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="Y">methods</QualifierName><QualifierName UI="Q000706" MajorTopicYN="N">statistics & numerical data</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="Y">genetics</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D012680" MajorTopicYN="N">Sensitivity and Specificity</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045169" MajorTopicYN="N">Severe Acute Respiratory Syndrome</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="N">diagnosis</QualifierName><QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName><QualifierName UI="Q000821" MajorTopicYN="N">virology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D013624" MajorTopicYN="N" Type="Geographic">Taiwan</DescriptorName><QualifierName UI="Q000453" MajorTopicYN="N">epidemiology</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2004</Year><Month>10</Month><Day>22</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2004</Year><Month>12</Month><Day>16</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2004</Year><Month>10</Month><Day>22</Day><Hour>9</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">15497005</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Chen, Yao Shen" sort="Chen, Yao Shen" uniqKey="Chen Y" first="Yao-Shen" last="Chen">Yao-Shen Chen</name><name sortKey="Hwang, Chun Kai" sort="Hwang, Chun Kai" uniqKey="Hwang C" first="Chun-Kai" last="Hwang">Chun-Kai Hwang</name><name sortKey="Lee, Susan Shin Jung" sort="Lee, Susan Shin Jung" uniqKey="Lee S" first="Susan Shin-Jung" last="Lee">Susan Shin-Jung Lee</name><name sortKey="Liu, Yung Ching" sort="Liu, Yung Ching" uniqKey="Liu Y" first="Yung-Ching" last="Liu">Yung-Ching Liu</name><name sortKey="Shih, Yi Li" sort="Shih, Yi Li" uniqKey="Shih Y" first="Yi-Li" last="Shih">Yi-Li Shih</name><name sortKey="Wang, Shiow Jen" sort="Wang, Shiow Jen" uniqKey="Wang S" first="Shiow-Jen" last="Wang">Shiow-Jen Wang</name><name sortKey="Wann, Shue Ren" sort="Wann, Shue Ren" uniqKey="Wann S" first="Shue-Ren" last="Wann">Shue-Ren Wann</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Lin, Hsi Hsun" sort="Lin, Hsi Hsun" uniqKey="Lin H" first="Hsi-Hsun" last="Lin">Hsi-Hsun Lin</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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