Small molecules targeting severe acute respiratory syndrome human coronavirus.
Identifieur interne : 000921 ( Ncbi/Merge ); précédent : 000920; suivant : 000922Small molecules targeting severe acute respiratory syndrome human coronavirus.
Auteurs : Chung-Yi Wu [Taïwan] ; Jia-Tsrong Jan ; Shiou-Hwa Ma ; Chih-Jung Kuo ; Hsueh-Fen Juan ; Yih-Shyun E. Cheng ; Hsien-Hua Hsu ; Hsuan-Cheng Huang ; Douglass Wu ; Ashraf Brik ; Fu-Sen Liang ; Rai-Shung Liu ; Jim-Min Fang ; Shui-Tein Chen ; Po-Huang Liang ; Chi-Huey WongSource :
- Proceedings of the National Academy of Sciences of the United States of America [ 0027-8424 ] ; 2004.
Descripteurs français
- KwdFr :
- MESH :
- antagonistes et inhibiteurs : Protéines virales.
- pharmacologie : Antiviraux.
- Animaux, Cellules Vero, Cysteine endopeptidases, Cytométrie en flux, Endopeptidases, Réplication virale, Technique d'immunofluorescence, Technique de Western, Test ELISA, Virus du SRAS.
English descriptors
- KwdEn :
- Animals, Antiviral Agents (pharmacology), Blotting, Western, Chlorocebus aethiops, Cysteine Endopeptidases, Endopeptidases, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Fluorescent Antibody Technique, SARS Virus (drug effects), Vero Cells, Viral Proteins (antagonists & inhibitors), Virus Replication (drug effects).
- MESH :
- chemical , antagonists & inhibitors : Viral Proteins.
- chemical , pharmacology : Antiviral Agents.
- drug effects : SARS Virus, Virus Replication.
- Animals, Blotting, Western, Chlorocebus aethiops, Cysteine Endopeptidases, Endopeptidases, Enzyme-Linked Immunosorbent Assay, Flow Cytometry, Fluorescent Antibody Technique, Vero Cells.
Abstract
Severe acute respiratory syndrome (SARS) is an infectious disease caused by a novel human coronavirus. Currently, no effective antiviral agents exist against this type of virus. A cell-based assay, with SARS virus and Vero E6 cells, was developed to screen existing drugs, natural products, and synthetic compounds to identify effective anti-SARS agents. Of >10,000 agents tested, approximately 50 compounds were found active at 10 microM; among these compounds, two are existing drugs (Reserpine 13 and Aescin 5) and several are in clinical development. These 50 active compounds were tested again, and compounds 2-6, 10, and 13 showed active at 3 microM. The 50% inhibitory concentrations for the inhibition of viral replication (EC(50)) and host growth (CC(50)) were then measured and the selectivity index (SI = CC(50)/EC(50)) was determined. The EC(50), based on ELISA, and SI for Reserpine, Aescim, and Valinomycin are 3.4 microM (SI = 7.3), 6.0 microM (SI = 2.5), and 0.85 microM (SI = 80), respectively. Additional studies were carried out to further understand the mode of action of some active compounds, including ELISA, Western blot analysis, immunofluorescence and flow cytometry assays, and inhibition against the 3CL protease and viral entry. Of particular interest are the two anti-HIV agents, one as an entry blocker and the other as a 3CL protease inhibitor (K(i) = 0.6 microM).
DOI: 10.1073/pnas.0403596101
PubMed: 15226499
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Cheng</name></author><author><name sortKey="Hsu, Hsien Hua" sort="Hsu, Hsien Hua" uniqKey="Hsu H" first="Hsien-Hua" last="Hsu">Hsien-Hua Hsu</name></author><author><name sortKey="Huang, Hsuan Cheng" sort="Huang, Hsuan Cheng" uniqKey="Huang H" first="Hsuan-Cheng" last="Huang">Hsuan-Cheng Huang</name></author><author><name sortKey="Wu, Douglass" sort="Wu, Douglass" uniqKey="Wu D" first="Douglass" last="Wu">Douglass Wu</name></author><author><name sortKey="Brik, Ashraf" sort="Brik, Ashraf" uniqKey="Brik A" first="Ashraf" last="Brik">Ashraf Brik</name></author><author><name sortKey="Liang, Fu Sen" sort="Liang, Fu Sen" uniqKey="Liang F" first="Fu-Sen" last="Liang">Fu-Sen Liang</name></author><author><name sortKey="Liu, Rai Shung" sort="Liu, Rai Shung" uniqKey="Liu R" first="Rai-Shung" last="Liu">Rai-Shung Liu</name></author><author><name sortKey="Fang, Jim Min" sort="Fang, Jim Min" uniqKey="Fang J" first="Jim-Min" last="Fang">Jim-Min Fang</name></author><author><name sortKey="Chen, Shui Tein" sort="Chen, Shui Tein" uniqKey="Chen S" first="Shui-Tein" last="Chen">Shui-Tein Chen</name></author><author><name sortKey="Liang, Po Huang" sort="Liang, Po Huang" uniqKey="Liang P" first="Po-Huang" last="Liang">Po-Huang Liang</name></author><author><name sortKey="Wong, Chi Huey" sort="Wong, Chi Huey" uniqKey="Wong C" first="Chi-Huey" last="Wong">Chi-Huey Wong</name></author></analytic><series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title><idno type="ISSN">0027-8424</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antiviral Agents (pharmacology)</term><term>Blotting, Western</term><term>Chlorocebus aethiops</term><term>Cysteine Endopeptidases</term><term>Endopeptidases</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Flow Cytometry</term><term>Fluorescent Antibody Technique</term><term>SARS Virus (drug effects)</term><term>Vero Cells</term><term>Viral Proteins (antagonists & inhibitors)</term><term>Virus Replication (drug effects)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Antiviraux (pharmacologie)</term><term>Cellules Vero</term><term>Cysteine endopeptidases</term><term>Cytométrie en flux</term><term>Endopeptidases</term><term>Protéines virales (antagonistes et inhibiteurs)</term><term>Réplication virale ()</term><term>Technique d'immunofluorescence</term><term>Technique de Western</term><term>Test ELISA</term><term>Virus du SRAS ()</term></keywords><keywords scheme="MESH" type="chemical" qualifier="antagonists & inhibitors" xml:lang="en"><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="pharmacology" xml:lang="en"><term>Antiviral Agents</term></keywords><keywords scheme="MESH" qualifier="antagonistes et inhibiteurs" xml:lang="fr"><term>Protéines virales</term></keywords><keywords scheme="MESH" qualifier="drug effects" xml:lang="en"><term>SARS Virus</term><term>Virus Replication</term></keywords><keywords scheme="MESH" qualifier="pharmacologie" xml:lang="fr"><term>Antiviraux</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Blotting, Western</term><term>Chlorocebus aethiops</term><term>Cysteine Endopeptidases</term><term>Endopeptidases</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Flow Cytometry</term><term>Fluorescent Antibody Technique</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Cysteine endopeptidases</term><term>Cytométrie en flux</term><term>Endopeptidases</term><term>Réplication virale</term><term>Technique d'immunofluorescence</term><term>Technique de Western</term><term>Test ELISA</term><term>Virus du SRAS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) is an infectious disease caused by a novel human coronavirus. Currently, no effective antiviral agents exist against this type of virus. A cell-based assay, with SARS virus and Vero E6 cells, was developed to screen existing drugs, natural products, and synthetic compounds to identify effective anti-SARS agents. Of >10,000 agents tested, approximately 50 compounds were found active at 10 microM; among these compounds, two are existing drugs (Reserpine 13 and Aescin 5) and several are in clinical development. These 50 active compounds were tested again, and compounds 2-6, 10, and 13 showed active at 3 microM. The 50% inhibitory concentrations for the inhibition of viral replication (EC(50)) and host growth (CC(50)) were then measured and the selectivity index (SI = CC(50)/EC(50)) was determined. The EC(50), based on ELISA, and SI for Reserpine, Aescim, and Valinomycin are 3.4 microM (SI = 7.3), 6.0 microM (SI = 2.5), and 0.85 microM (SI = 80), respectively. Additional studies were carried out to further understand the mode of action of some active compounds, including ELISA, Western blot analysis, immunofluorescence and flow cytometry assays, and inhibition against the 3CL protease and viral entry. Of particular interest are the two anti-HIV agents, one as an entry blocker and the other as a 3CL protease inhibitor (K(i) = 0.6 microM).</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">15226499</PMID><DateCompleted><Year>2004</Year><Month>07</Month><Day>30</Day></DateCompleted><DateRevised><Year>2020</Year><Month>03</Month><Day>25</Day></DateRevised><Article PubModel="Print-Electronic"><Journal><ISSN IssnType="Print">0027-8424</ISSN><JournalIssue CitedMedium="Print"><Volume>101</Volume><Issue>27</Issue><PubDate><Year>2004</Year><Month>Jul</Month><Day>06</Day></PubDate></JournalIssue><Title>Proceedings of the National Academy of Sciences of the United States of America</Title><ISOAbbreviation>Proc. Natl. Acad. Sci. U.S.A.</ISOAbbreviation></Journal><ArticleTitle>Small molecules targeting severe acute respiratory syndrome human coronavirus.</ArticleTitle><Pagination><MedlinePgn>10012-7</MedlinePgn></Pagination><Abstract><AbstractText>Severe acute respiratory syndrome (SARS) is an infectious disease caused by a novel human coronavirus. Currently, no effective antiviral agents exist against this type of virus. A cell-based assay, with SARS virus and Vero E6 cells, was developed to screen existing drugs, natural products, and synthetic compounds to identify effective anti-SARS agents. Of >10,000 agents tested, approximately 50 compounds were found active at 10 microM; among these compounds, two are existing drugs (Reserpine 13 and Aescin 5) and several are in clinical development. These 50 active compounds were tested again, and compounds 2-6, 10, and 13 showed active at 3 microM. The 50% inhibitory concentrations for the inhibition of viral replication (EC(50)) and host growth (CC(50)) were then measured and the selectivity index (SI = CC(50)/EC(50)) was determined. The EC(50), based on ELISA, and SI for Reserpine, Aescim, and Valinomycin are 3.4 microM (SI = 7.3), 6.0 microM (SI = 2.5), and 0.85 microM (SI = 80), respectively. Additional studies were carried out to further understand the mode of action of some active compounds, including ELISA, Western blot analysis, immunofluorescence and flow cytometry assays, and inhibition against the 3CL protease and viral entry. Of particular interest are the two anti-HIV agents, one as an entry blocker and the other as a 3CL protease inhibitor (K(i) = 0.6 microM).</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Chung-Yi</ForeName><Initials>CY</Initials><AffiliationInfo><Affiliation>Genomics Research Center and Institute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Jan</LastName><ForeName>Jia-Tsrong</ForeName><Initials>JT</Initials></Author><Author ValidYN="Y"><LastName>Ma</LastName><ForeName>Shiou-Hwa</ForeName><Initials>SH</Initials></Author><Author ValidYN="Y"><LastName>Kuo</LastName><ForeName>Chih-Jung</ForeName><Initials>CJ</Initials></Author><Author ValidYN="Y"><LastName>Juan</LastName><ForeName>Hsueh-Fen</ForeName><Initials>HF</Initials></Author><Author ValidYN="Y"><LastName>Cheng</LastName><ForeName>Yih-Shyun E</ForeName><Initials>YS</Initials></Author><Author ValidYN="Y"><LastName>Hsu</LastName><ForeName>Hsien-Hua</ForeName><Initials>HH</Initials></Author><Author ValidYN="Y"><LastName>Huang</LastName><ForeName>Hsuan-Cheng</ForeName><Initials>HC</Initials></Author><Author ValidYN="Y"><LastName>Wu</LastName><ForeName>Douglass</ForeName><Initials>D</Initials></Author><Author ValidYN="Y"><LastName>Brik</LastName><ForeName>Ashraf</ForeName><Initials>A</Initials></Author><Author ValidYN="Y"><LastName>Liang</LastName><ForeName>Fu-Sen</ForeName><Initials>FS</Initials></Author><Author ValidYN="Y"><LastName>Liu</LastName><ForeName>Rai-Shung</ForeName><Initials>RS</Initials></Author><Author ValidYN="Y"><LastName>Fang</LastName><ForeName>Jim-Min</ForeName><Initials>JM</Initials></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Shui-Tein</ForeName><Initials>ST</Initials></Author><Author ValidYN="Y"><LastName>Liang</LastName><ForeName>Po-Huang</ForeName><Initials>PH</Initials></Author><Author ValidYN="Y"><LastName>Wong</LastName><ForeName>Chi-Huey</ForeName><Initials>CH</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList><ArticleDate DateType="Electronic"><Year>2004</Year><Month>06</Month><Day>28</Day></ArticleDate></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>Proc Natl Acad Sci U S A</MedlineTA><NlmUniqueID>7505876</NlmUniqueID><ISSNLinking>0027-8424</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000998">Antiviral Agents</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014764">Viral Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.4.-</RegistryNumber><NameOfSubstance UI="D010450">Endopeptidases</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.4.22.-</RegistryNumber><NameOfSubstance UI="C099456">3C-like proteinase, Coronavirus</NameOfSubstance></Chemical><Chemical><RegistryNumber>EC 3.4.22.-</RegistryNumber><NameOfSubstance UI="D003546">Cysteine Endopeptidases</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000818" MajorTopicYN="N">Animals</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D000998" MajorTopicYN="N">Antiviral Agents</DescriptorName><QualifierName UI="Q000494" MajorTopicYN="Y">pharmacology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D015153" MajorTopicYN="N">Blotting, Western</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D002522" MajorTopicYN="N">Chlorocebus aethiops</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D003546" MajorTopicYN="N">Cysteine Endopeptidases</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D010450" MajorTopicYN="N">Endopeptidases</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004797" MajorTopicYN="N">Enzyme-Linked Immunosorbent Assay</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005434" MajorTopicYN="N">Flow Cytometry</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D005455" MajorTopicYN="N">Fluorescent Antibody Technique</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000187" MajorTopicYN="Y">drug effects</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014709" MajorTopicYN="N">Vero Cells</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D014764" MajorTopicYN="N">Viral Proteins</DescriptorName><QualifierName UI="Q000037" MajorTopicYN="N">antagonists & inhibitors</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014779" 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IdType="pubmed">10744007</ArticleId></ArticleIdList></Reference><Reference><Citation>Pharmacol Res. 2001 Sep;44(3):183-93</Citation><ArticleIdList><ArticleId IdType="pubmed">11529685</ArticleId></ArticleIdList></Reference><Reference><Citation>Chem Biol. 2002 Aug;9(8):891-6</Citation><ArticleIdList><ArticleId IdType="pubmed">12204688</ArticleId></ArticleIdList></Reference><Reference><Citation>Arch Pharm Res. 2003 Jan;26(1):58-63</Citation><ArticleIdList><ArticleId IdType="pubmed">12568360</ArticleId></ArticleIdList></Reference><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1995-2005</Citation><ArticleIdList><ArticleId IdType="pubmed">12671061</ArticleId></ArticleIdList></Reference><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1977-85</Citation><ArticleIdList><ArticleId IdType="pubmed">12671062</ArticleId></ArticleIdList></Reference><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1986-94</Citation><ArticleIdList><ArticleId IdType="pubmed">12682352</ArticleId></ArticleIdList></Reference><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1953-66</Citation><ArticleIdList><ArticleId IdType="pubmed">12690092</ArticleId></ArticleIdList></Reference><Reference><Citation>Lancet. 2003 Apr 19;361(9366):1319-25</Citation><ArticleIdList><ArticleId IdType="pubmed">12711465</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2003 May 30;300(5624):1394-9</Citation><ArticleIdList><ArticleId IdType="pubmed">12730500</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2003 May 30;300(5624):1399-404</Citation><ArticleIdList><ArticleId IdType="pubmed">12730501</ArticleId></ArticleIdList></Reference><Reference><Citation>JAMA. 2003 Jun 4;289(21):2801-9</Citation><ArticleIdList><ArticleId IdType="pubmed">12734147</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2003 Jun 13;300(5626):1763-7</Citation><ArticleIdList><ArticleId IdType="pubmed">12746549</ArticleId></ArticleIdList></Reference><Reference><Citation>Lancet. 2003 May 10;361(9369):1615-7</Citation><ArticleIdList><ArticleId IdType="pubmed">12747883</ArticleId></ArticleIdList></Reference><Reference><Citation>Lancet. 2003 May 24;361(9371):1767-72</Citation><ArticleIdList><ArticleId IdType="pubmed">12781535</ArticleId></ArticleIdList></Reference><Reference><Citation>Lancet. 2003 May 24;361(9371):1779-85</Citation><ArticleIdList><ArticleId IdType="pubmed">12781537</ArticleId></ArticleIdList></Reference><Reference><Citation>Lancet. 2003 Jun 14;361(9374):2045-6</Citation><ArticleIdList><ArticleId IdType="pubmed">12814717</ArticleId></ArticleIdList></Reference><Reference><Citation>Chin J Physiol. 2003 Mar 31;46(1):1-7</Citation><ArticleIdList><ArticleId IdType="pubmed">12817698</ArticleId></ArticleIdList></Reference><Reference><Citation>J Pineal Res. 2003 Aug;35(1):69-70</Citation><ArticleIdList><ArticleId IdType="pubmed">12823616</ArticleId></ArticleIdList></Reference><Reference><Citation>Med Sci Monit. 2003 Jun;9(6):SR29-34</Citation><ArticleIdList><ArticleId IdType="pubmed">12824965</ArticleId></ArticleIdList></Reference><Reference><Citation>Thorax. 2003 Aug;58(8):686-9</Citation><ArticleIdList><ArticleId IdType="pubmed">12885985</ArticleId></ArticleIdList></Reference><Reference><Citation>Lancet. 2003 Jul 26;362(9380):293-4</Citation><ArticleIdList><ArticleId IdType="pubmed">12892961</ArticleId></ArticleIdList></Reference><Reference><Citation>Org Biomol Chem. 2003 Jan 7;1(1):5-14</Citation><ArticleIdList><ArticleId IdType="pubmed">12929379</ArticleId></ArticleIdList></Reference><Reference><Citation>Am J Respir Crit Care Med. 2003 Dec 15;168(12):1449-56</Citation><ArticleIdList><ArticleId IdType="pubmed">12947028</ArticleId></ArticleIdList></Reference><Reference><Citation>Emerg Infect Dis. 2003 Sep;9(9):1064-9</Citation><ArticleIdList><ArticleId IdType="pubmed">14519241</ArticleId></ArticleIdList></Reference><Reference><Citation>Angew Chem Int Ed Engl. 2003 Oct 6;42(38):4661-4</Citation><ArticleIdList><ArticleId IdType="pubmed">14533157</ArticleId></ArticleIdList></Reference><Reference><Citation>J Biol Chem. 2004 Jan 16;279(3):1637-42</Citation><ArticleIdList><ArticleId IdType="pubmed">14561748</ArticleId></ArticleIdList></Reference><Reference><Citation>Proc Natl Acad Sci U S A. 2003 Nov 11;100(23):13190-5</Citation><ArticleIdList><ArticleId IdType="pubmed">14585926</ArticleId></ArticleIdList></Reference><Reference><Citation>J Antimicrob Chemother. 2003 Dec;52(6):1049-50</Citation><ArticleIdList><ArticleId IdType="pubmed">14613951</ArticleId></ArticleIdList></Reference><Reference><Citation>Nature. 2003 Nov 27;426(6965):450-4</Citation><ArticleIdList><ArticleId IdType="pubmed">14647384</ArticleId></ArticleIdList></Reference><Reference><Citation>Arch Pharm Res. 2003 Nov;26(11):906-11</Citation><ArticleIdList><ArticleId IdType="pubmed">14661855</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2004 Mar 12;303(5664):1666-9</Citation><ArticleIdList><ArticleId IdType="pubmed">14752165</ArticleId></ArticleIdList></Reference><Reference><Citation>Curr Opin Investig Drugs. 2004 Feb;5(2):179-85</Citation><ArticleIdList><ArticleId IdType="pubmed">15043392</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochemistry. 2004 Apr 20;43(15):4568-74</Citation><ArticleIdList><ArticleId IdType="pubmed">15078103</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochemistry. 2004 May 4;43(17):4906-12</Citation><ArticleIdList><ArticleId IdType="pubmed">15109248</ArticleId></ArticleIdList></Reference><Reference><Citation>Biochem Biophys Res Commun. 2004 Jun 11;318(4):862-7</Citation><ArticleIdList><ArticleId IdType="pubmed">15147951</ArticleId></ArticleIdList></Reference><Reference><Citation>Emerg Infect Dis. 2004 Apr;10(4):581-6</Citation><ArticleIdList><ArticleId IdType="pubmed">15200845</ArticleId></ArticleIdList></Reference><Reference><Citation>Haematologica. 1991 Nov-Dec;76(6):491-9</Citation><ArticleIdList><ArticleId IdType="pubmed">1820986</ArticleId></ArticleIdList></Reference><Reference><Citation>Eur J Pharmacol. 1974 Aug;27(3):349-54</Citation><ArticleIdList><ArticleId IdType="pubmed">4422525</ArticleId></ArticleIdList></Reference><Reference><Citation>Contraception. 1984 Oct;30(4):363-9</Citation><ArticleIdList><ArticleId IdType="pubmed">6548951</ArticleId></ArticleIdList></Reference><Reference><Citation>Med Klin. 1977 Jan 28;72(4):128-34</Citation><ArticleIdList><ArticleId IdType="pubmed">840133</ArticleId></ArticleIdList></Reference><Reference><Citation>J Immunol Methods. 1993 Jan 4;157(1-2):233-40</Citation><ArticleIdList><ArticleId IdType="pubmed">8423368</ArticleId></ArticleIdList></Reference><Reference><Citation>J Interferon Cytokine Res. 1996 Jan;16(1):31-3</Citation><ArticleIdList><ArticleId IdType="pubmed">8640448</ArticleId></ArticleIdList></Reference><Reference><Citation>Clin Pharmacol Ther. 1996 Oct;60(4):368-73</Citation><ArticleIdList><ArticleId IdType="pubmed">8873684</ArticleId></ArticleIdList></Reference><Reference><Citation>Virology. 1998 May 10;244(2):530-41</Citation><ArticleIdList><ArticleId IdType="pubmed">9601521</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>Taïwan</li></country></list><tree><noCountry><name sortKey="Brik, Ashraf" sort="Brik, Ashraf" uniqKey="Brik A" first="Ashraf" last="Brik">Ashraf Brik</name><name sortKey="Chen, Shui Tein" sort="Chen, Shui Tein" uniqKey="Chen S" first="Shui-Tein" last="Chen">Shui-Tein Chen</name><name sortKey="Cheng, Yih Shyun E" sort="Cheng, Yih Shyun E" uniqKey="Cheng Y" first="Yih-Shyun E" last="Cheng">Yih-Shyun E. Cheng</name><name sortKey="Fang, Jim Min" sort="Fang, Jim Min" uniqKey="Fang J" first="Jim-Min" last="Fang">Jim-Min Fang</name><name sortKey="Hsu, Hsien Hua" sort="Hsu, Hsien Hua" uniqKey="Hsu H" first="Hsien-Hua" last="Hsu">Hsien-Hua Hsu</name><name sortKey="Huang, Hsuan Cheng" sort="Huang, Hsuan Cheng" uniqKey="Huang H" first="Hsuan-Cheng" last="Huang">Hsuan-Cheng Huang</name><name sortKey="Jan, Jia Tsrong" sort="Jan, Jia Tsrong" uniqKey="Jan J" first="Jia-Tsrong" last="Jan">Jia-Tsrong Jan</name><name sortKey="Juan, Hsueh Fen" sort="Juan, Hsueh Fen" uniqKey="Juan H" first="Hsueh-Fen" last="Juan">Hsueh-Fen Juan</name><name sortKey="Kuo, Chih Jung" sort="Kuo, Chih Jung" uniqKey="Kuo C" first="Chih-Jung" last="Kuo">Chih-Jung Kuo</name><name sortKey="Liang, Fu Sen" sort="Liang, Fu Sen" uniqKey="Liang F" first="Fu-Sen" last="Liang">Fu-Sen Liang</name><name sortKey="Liang, Po Huang" sort="Liang, Po Huang" uniqKey="Liang P" first="Po-Huang" last="Liang">Po-Huang Liang</name><name sortKey="Liu, Rai Shung" sort="Liu, Rai Shung" uniqKey="Liu R" first="Rai-Shung" last="Liu">Rai-Shung Liu</name><name sortKey="Ma, Shiou Hwa" sort="Ma, Shiou Hwa" uniqKey="Ma S" first="Shiou-Hwa" last="Ma">Shiou-Hwa Ma</name><name sortKey="Wong, Chi Huey" sort="Wong, Chi Huey" uniqKey="Wong C" first="Chi-Huey" last="Wong">Chi-Huey Wong</name><name sortKey="Wu, Douglass" sort="Wu, Douglass" uniqKey="Wu D" first="Douglass" last="Wu">Douglass Wu</name></noCountry><country name="Taïwan"><noRegion><name sortKey="Wu, Chung Yi" sort="Wu, Chung Yi" uniqKey="Wu C" first="Chung-Yi" last="Wu">Chung-Yi Wu</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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