[Expression and purification of recombinant N-terminal protein of SARS S1 subunit expressed in E. Coli].
Identifieur interne : 000616 ( Ncbi/Merge ); précédent : 000615; suivant : 000617[Expression and purification of recombinant N-terminal protein of SARS S1 subunit expressed in E. Coli].
Auteurs : Xiao-Ang Yang [République populaire de Chine] ; Xue-Yuan Dong ; Yan Li ; Yun-Yan Li ; Guo-Bin Xu ; Zhen-Ru Feng ; Yue-Dan Wang ; Wei-Feng ChenSource :
- Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences [ 1671-167X ] ; 2004.
Descripteurs français
- KwdFr :
- Escherichia coli (génétique), Humains, Protéines recombinantes (biosynthèse), Protéines recombinantes (isolement et purification), Protéines virales (biosynthèse), Protéines virales (immunologie), Protéines virales (isolement et purification), Sous-unités de protéines, Technique de Western, Virus du SRAS (), Virus du SRAS (immunologie).
- MESH :
- biosynthèse : Protéines recombinantes, Protéines virales.
- génétique : Escherichia coli.
- immunologie : Protéines virales, Virus du SRAS.
- isolement et purification : Protéines recombinantes, Protéines virales.
- Humains, Sous-unités de protéines, Technique de Western, Virus du SRAS.
English descriptors
- KwdEn :
- Blotting, Western, Escherichia coli (genetics), Humans, Protein Subunits, Recombinant Proteins (biosynthesis), Recombinant Proteins (isolation & purification), SARS Virus (chemistry), SARS Virus (immunology), Viral Proteins (biosynthesis), Viral Proteins (immunology), Viral Proteins (isolation & purification).
- MESH :
- chemical , biosynthesis : Recombinant Proteins, Viral Proteins.
- chemical , immunology : Viral Proteins.
- chemical , isolation & purification : Recombinant Proteins, Viral Proteins.
- chemical : Protein Subunits.
- chemistry : SARS Virus.
- genetics : Escherichia coli.
- immunology : SARS Virus.
- Blotting, Western, Humans.
Abstract
To express and purify the recombinant N-terminal protein of SARS virus S1 subunit and to study its role in SARS immune response.
PubMed: 14970895
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pubmed:14970895Le document en format XML
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Coli].</title><author><name sortKey="Yang, Xiao Ang" sort="Yang, Xiao Ang" uniqKey="Yang X" first="Xiao-Ang" last="Yang">Xiao-Ang Yang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Immunology, Peking University School of Basic Medical Sciences, Beijing 100083, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Immunology, Peking University School of Basic Medical Sciences, Beijing 100083</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Dong, Xue Yuan" sort="Dong, Xue Yuan" uniqKey="Dong X" first="Xue-Yuan" last="Dong">Xue-Yuan Dong</name></author><author><name sortKey="Li, Yan" sort="Li, Yan" uniqKey="Li Y" first="Yan" last="Li">Yan Li</name></author><author><name sortKey="Li, Yun Yan" sort="Li, Yun Yan" uniqKey="Li Y" first="Yun-Yan" last="Li">Yun-Yan Li</name></author><author><name sortKey="Xu, Guo Bin" sort="Xu, Guo Bin" uniqKey="Xu G" first="Guo-Bin" last="Xu">Guo-Bin Xu</name></author><author><name sortKey="Feng, Zhen Ru" sort="Feng, Zhen Ru" uniqKey="Feng Z" first="Zhen-Ru" last="Feng">Zhen-Ru Feng</name></author><author><name sortKey="Wang, Yue Dan" sort="Wang, Yue Dan" uniqKey="Wang Y" first="Yue-Dan" last="Wang">Yue-Dan Wang</name></author><author><name sortKey="Chen, Wei Feng" sort="Chen, Wei Feng" uniqKey="Chen W" first="Wei-Feng" last="Chen">Wei-Feng Chen</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2004">2004</date><idno type="RBID">pubmed:14970895</idno><idno type="pmid">14970895</idno><idno type="wicri:Area/PubMed/Corpus">002F49</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002F49</idno><idno type="wicri:Area/PubMed/Curation">002F49</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002F49</idno><idno type="wicri:Area/PubMed/Checkpoint">002910</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002910</idno><idno type="wicri:Area/Ncbi/Merge">000616</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">[Expression and purification of recombinant N-terminal protein of SARS S1 subunit expressed in E. Coli].</title><author><name sortKey="Yang, Xiao Ang" sort="Yang, Xiao Ang" uniqKey="Yang X" first="Xiao-Ang" last="Yang">Xiao-Ang Yang</name><affiliation wicri:level="1"><nlm:affiliation>Department of Immunology, Peking University School of Basic Medical Sciences, Beijing 100083, China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Department of Immunology, Peking University School of Basic Medical Sciences, Beijing 100083</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Dong, Xue Yuan" sort="Dong, Xue Yuan" uniqKey="Dong X" first="Xue-Yuan" last="Dong">Xue-Yuan Dong</name></author><author><name sortKey="Li, Yan" sort="Li, Yan" uniqKey="Li Y" first="Yan" last="Li">Yan Li</name></author><author><name sortKey="Li, Yun Yan" sort="Li, Yun Yan" uniqKey="Li Y" first="Yun-Yan" last="Li">Yun-Yan Li</name></author><author><name sortKey="Xu, Guo Bin" sort="Xu, Guo Bin" uniqKey="Xu G" first="Guo-Bin" last="Xu">Guo-Bin Xu</name></author><author><name sortKey="Feng, Zhen Ru" sort="Feng, Zhen Ru" uniqKey="Feng Z" first="Zhen-Ru" last="Feng">Zhen-Ru Feng</name></author><author><name sortKey="Wang, Yue Dan" sort="Wang, Yue Dan" uniqKey="Wang Y" first="Yue-Dan" last="Wang">Yue-Dan Wang</name></author><author><name sortKey="Chen, Wei Feng" sort="Chen, Wei Feng" uniqKey="Chen W" first="Wei-Feng" last="Chen">Wei-Feng Chen</name></author></analytic><series><title level="j">Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences</title><idno type="ISSN">1671-167X</idno><imprint><date when="2004" type="published">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Blotting, Western</term><term>Escherichia coli (genetics)</term><term>Humans</term><term>Protein Subunits</term><term>Recombinant Proteins (biosynthesis)</term><term>Recombinant Proteins (isolation & purification)</term><term>SARS Virus (chemistry)</term><term>SARS Virus (immunology)</term><term>Viral Proteins (biosynthesis)</term><term>Viral Proteins (immunology)</term><term>Viral Proteins (isolation & purification)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Escherichia coli (génétique)</term><term>Humains</term><term>Protéines recombinantes (biosynthèse)</term><term>Protéines recombinantes (isolement et purification)</term><term>Protéines virales (biosynthèse)</term><term>Protéines virales (immunologie)</term><term>Protéines virales (isolement et purification)</term><term>Sous-unités de protéines</term><term>Technique de Western</term><term>Virus du SRAS ()</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Recombinant Proteins</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Recombinant Proteins</term><term>Viral Proteins</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Protein Subunits</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Protéines recombinantes</term><term>Protéines virales</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Protéines virales</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Protéines recombinantes</term><term>Protéines virales</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Blotting, Western</term><term>Humans</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Humains</term><term>Sous-unités de protéines</term><term>Technique de Western</term><term>Virus du SRAS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">To express and purify the recombinant N-terminal protein of SARS virus S1 subunit and to study its role in SARS immune response.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">14970895</PMID><DateCompleted><Year>2004</Year><Month>06</Month><Day>21</Day></DateCompleted><DateRevised><Year>2017</Year><Month>11</Month><Day>16</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">1671-167X</ISSN><JournalIssue CitedMedium="Print"><Volume>36</Volume><Issue>1</Issue><PubDate><Year>2004</Year><Month>Feb</Month></PubDate></JournalIssue><Title>Beijing da xue xue bao. Yi xue ban = Journal of Peking University. Health sciences</Title><ISOAbbreviation>Beijing Da Xue Xue Bao</ISOAbbreviation></Journal><ArticleTitle>[Expression and purification of recombinant N-terminal protein of SARS S1 subunit expressed in E. Coli].</ArticleTitle><Pagination><MedlinePgn>79-81</MedlinePgn></Pagination><Abstract><AbstractText Label="OBJECTIVE" NlmCategory="OBJECTIVE">To express and purify the recombinant N-terminal protein of SARS virus S1 subunit and to study its role in SARS immune response.</AbstractText><AbstractText Label="METHODS" NlmCategory="METHODS">The gene encoding N-terminal 334 amino acid residuals of SARS virus S1 subunit was cloned and expressed in E. Coli. After purification, the recombinant protein was identified by anti-SARS positive sera from recovered SARS patients. The sera from health donors, which were collected before the out-break of SARS, were used as negative control in the study.</AbstractText><AbstractText Label="RESULTS" NlmCategory="RESULTS">Sequencing analysis confirmed that the desired DNA sequence in recombinant plasmid was correct and had the same sequence of natural N-terminal of SARS virus S1 subunit. The molecular weight of recombinant fusion protein is about 64 000. The recombinant S1 protein could react with three antibody positive samples from recovered SARS patients, which showed specific bands at 64 000, but not with the control samples according to results of western blot.</AbstractText><AbstractText Label="CONCLUSION" NlmCategory="CONCLUSIONS">The recombinant N-terminal protein of SARS virus S1 subunit displays specific reaction with SARS antibody and may provide a good tool for further research of immune response to SARS virus.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Yang</LastName><ForeName>Xiao-Ang</ForeName><Initials>XA</Initials><AffiliationInfo><Affiliation>Department of Immunology, Peking University School of Basic Medical Sciences, Beijing 100083, China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Dong</LastName><ForeName>Xue-Yuan</ForeName><Initials>XY</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Yan</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Yun-Yan</ForeName><Initials>YY</Initials></Author><Author ValidYN="Y"><LastName>Xu</LastName><ForeName>Guo-Bin</ForeName><Initials>GB</Initials></Author><Author ValidYN="Y"><LastName>Feng</LastName><ForeName>Zhen-Ru</ForeName><Initials>ZR</Initials></Author><Author ValidYN="Y"><LastName>Wang</LastName><ForeName>Yue-Dan</ForeName><Initials>YD</Initials></Author><Author ValidYN="Y"><LastName>Chen</LastName><ForeName>Wei-Feng</ForeName><Initials>WF</Initials></Author></AuthorList><Language>chi</Language><PublicationTypeList><PublicationType UI="D004740">English Abstract</PublicationType><PublicationType UI="D016428">Journal Article</PublicationType><PublicationType UI="D013485">Research Support, Non-U.S. Gov't</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>China</Country><MedlineTA>Beijing Da Xue Xue Bao Yi Xue Ban</MedlineTA><NlmUniqueID>101125284</NlmUniqueID><ISSNLinking>1671-167X</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D021122">Protein Subunits</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D011994">Recombinant Proteins</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D014764">Viral Proteins</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D015153" MajorTopicYN="N">Blotting, Western</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004926" MajorTopicYN="N">Escherichia coli</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D021122" MajorTopicYN="N">Protein Subunits</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D011994" MajorTopicYN="N">Recombinant Proteins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000737" MajorTopicYN="Y">chemistry</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D014764" MajorTopicYN="N">Viral Proteins</DescriptorName><QualifierName UI="Q000096" MajorTopicYN="Y">biosynthesis</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000302" MajorTopicYN="N">isolation & purification</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2004</Year><Month>2</Month><Day>19</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2004</Year><Month>6</Month><Day>23</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2004</Year><Month>2</Month><Day>19</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">14970895</ArticleId></ArticleIdList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><noCountry><name sortKey="Chen, Wei Feng" sort="Chen, Wei Feng" uniqKey="Chen W" first="Wei-Feng" last="Chen">Wei-Feng Chen</name><name sortKey="Dong, Xue Yuan" sort="Dong, Xue Yuan" uniqKey="Dong X" first="Xue-Yuan" last="Dong">Xue-Yuan Dong</name><name sortKey="Feng, Zhen Ru" sort="Feng, Zhen Ru" uniqKey="Feng Z" first="Zhen-Ru" last="Feng">Zhen-Ru Feng</name><name sortKey="Li, Yan" sort="Li, Yan" uniqKey="Li Y" first="Yan" last="Li">Yan Li</name><name sortKey="Li, Yun Yan" sort="Li, Yun Yan" uniqKey="Li Y" first="Yun-Yan" last="Li">Yun-Yan Li</name><name sortKey="Wang, Yue Dan" sort="Wang, Yue Dan" uniqKey="Wang Y" first="Yue-Dan" last="Wang">Yue-Dan Wang</name><name sortKey="Xu, Guo Bin" sort="Xu, Guo Bin" uniqKey="Xu G" first="Guo-Bin" last="Xu">Guo-Bin Xu</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Yang, Xiao Ang" sort="Yang, Xiao Ang" uniqKey="Yang X" first="Xiao-Ang" last="Yang">Xiao-Ang Yang</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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