Diagnosis of severe acute respiratory syndrome (SARS) by detection of SARS coronavirus nucleocapsid antibodies in an antigen-capturing enzyme-linked immunosorbent assay.
Identifieur interne : 000492 ( Ncbi/Merge ); précédent : 000491; suivant : 000493Diagnosis of severe acute respiratory syndrome (SARS) by detection of SARS coronavirus nucleocapsid antibodies in an antigen-capturing enzyme-linked immunosorbent assay.
Auteurs : Yuling Shi [République populaire de Chine] ; Yanping Yi ; Ping Li ; Tieji Kuang ; Linhai Li ; Mei Dong ; Qingjun Ma ; Cheng CaoSource :
- Journal of clinical microbiology [ 0095-1137 ] ; 2003.
Descripteurs français
- KwdFr :
- Amorces ADN, Anticorps antiviraux, Humains, Nucléocapside (génétique), Nucléocapside (immunologie), Réaction de polymérisation en chaîne, Syndrome respiratoire aigu sévère (diagnostic), Séquence nucléotidique, Test ELISA (), Virus du SRAS (génétique), Virus du SRAS (immunologie), Virus du SRAS (isolement et purification).
- MESH :
- diagnostic : Syndrome respiratoire aigu sévère.
- génétique : Nucléocapside, Virus du SRAS.
- immunologie : Nucléocapside, Virus du SRAS.
- isolement et purification : Virus du SRAS.
- Amorces ADN, Anticorps antiviraux, Humains, Réaction de polymérisation en chaîne, Séquence nucléotidique, Test ELISA.
English descriptors
- KwdEn :
- Antibodies, Viral, Base Sequence, DNA Primers, Enzyme-Linked Immunosorbent Assay (methods), Humans, Nucleocapsid (genetics), Nucleocapsid (immunology), Polymerase Chain Reaction, SARS Virus (genetics), SARS Virus (immunology), SARS Virus (isolation & purification), Severe Acute Respiratory Syndrome (diagnosis).
- MESH :
- chemical : Antibodies, Viral, DNA Primers.
- diagnosis : Severe Acute Respiratory Syndrome.
- genetics : Nucleocapsid, SARS Virus.
- immunology : Nucleocapsid, SARS Virus.
- isolation & purification : SARS Virus.
- methods : Enzyme-Linked Immunosorbent Assay.
- Base Sequence, Humans, Polymerase Chain Reaction.
Abstract
Recombinant severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein was employed to establish an antigen-capturing enzyme-linked immunosorbent assay (ELISA). Antinucleocapsid protein antibodies could be detected in 68.4% of probable SARS patients 6 to 10 days after illness and in 89.6% of the patients 11 to 61 days after illness. No false-positive results were observed in 20 non-SARS fever patients, 24 non-SARS respiratory illness patients, and 20 health care workers. Among 940 other non-SARS clinical serum samples, only 1 was found to be weakly positive. This method provides a new, sensitive, and specific approach for SARS diagnosis.
DOI: 10.1128/jcm.41.12.5781-5782.2003
PubMed: 14662982
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 003054
- to stream PubMed, to step Curation: 003054
- to stream PubMed, to step Checkpoint: 003275
Links to Exploration step
pubmed:14662982Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Diagnosis of severe acute respiratory syndrome (SARS) by detection of SARS coronavirus nucleocapsid antibodies in an antigen-capturing enzyme-linked immunosorbent assay.</title><author><name sortKey="Shi, Yuling" sort="Shi, Yuling" uniqKey="Shi Y" first="Yuling" last="Shi">Yuling Shi</name><affiliation wicri:level="1"><nlm:affiliation>Beijing Institute of Biotechnology, Beijing 100850, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Institute of Biotechnology, Beijing 100850</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Yi, Yanping" sort="Yi, Yanping" uniqKey="Yi Y" first="Yanping" last="Yi">Yanping Yi</name></author><author><name sortKey="Li, Ping" sort="Li, Ping" uniqKey="Li P" first="Ping" last="Li">Ping Li</name></author><author><name sortKey="Kuang, Tieji" sort="Kuang, Tieji" uniqKey="Kuang T" first="Tieji" last="Kuang">Tieji Kuang</name></author><author><name sortKey="Li, Linhai" sort="Li, Linhai" uniqKey="Li L" first="Linhai" last="Li">Linhai Li</name></author><author><name sortKey="Dong, Mei" sort="Dong, Mei" uniqKey="Dong M" first="Mei" last="Dong">Mei Dong</name></author><author><name sortKey="Ma, Qingjun" sort="Ma, Qingjun" uniqKey="Ma Q" first="Qingjun" last="Ma">Qingjun Ma</name></author><author><name sortKey="Cao, Cheng" sort="Cao, Cheng" uniqKey="Cao C" first="Cheng" last="Cao">Cheng Cao</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2003">2003</date><idno type="RBID">pubmed:14662982</idno><idno type="pmid">14662982</idno><idno type="doi">10.1128/jcm.41.12.5781-5782.2003</idno><idno type="wicri:Area/PubMed/Corpus">003054</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">003054</idno><idno type="wicri:Area/PubMed/Curation">003054</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">003054</idno><idno type="wicri:Area/PubMed/Checkpoint">003275</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">003275</idno><idno type="wicri:Area/Ncbi/Merge">000492</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Diagnosis of severe acute respiratory syndrome (SARS) by detection of SARS coronavirus nucleocapsid antibodies in an antigen-capturing enzyme-linked immunosorbent assay.</title><author><name sortKey="Shi, Yuling" sort="Shi, Yuling" uniqKey="Shi Y" first="Yuling" last="Shi">Yuling Shi</name><affiliation wicri:level="1"><nlm:affiliation>Beijing Institute of Biotechnology, Beijing 100850, People's Republic of China.</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Beijing Institute of Biotechnology, Beijing 100850</wicri:regionArea><placeName><settlement type="city">Pékin</settlement></placeName></affiliation></author><author><name sortKey="Yi, Yanping" sort="Yi, Yanping" uniqKey="Yi Y" first="Yanping" last="Yi">Yanping Yi</name></author><author><name sortKey="Li, Ping" sort="Li, Ping" uniqKey="Li P" first="Ping" last="Li">Ping Li</name></author><author><name sortKey="Kuang, Tieji" sort="Kuang, Tieji" uniqKey="Kuang T" first="Tieji" last="Kuang">Tieji Kuang</name></author><author><name sortKey="Li, Linhai" sort="Li, Linhai" uniqKey="Li L" first="Linhai" last="Li">Linhai Li</name></author><author><name sortKey="Dong, Mei" sort="Dong, Mei" uniqKey="Dong M" first="Mei" last="Dong">Mei Dong</name></author><author><name sortKey="Ma, Qingjun" sort="Ma, Qingjun" uniqKey="Ma Q" first="Qingjun" last="Ma">Qingjun Ma</name></author><author><name sortKey="Cao, Cheng" sort="Cao, Cheng" uniqKey="Cao C" first="Cheng" last="Cao">Cheng Cao</name></author></analytic><series><title level="j">Journal of clinical microbiology</title><idno type="ISSN">0095-1137</idno><imprint><date when="2003" type="published">2003</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Antibodies, Viral</term><term>Base Sequence</term><term>DNA Primers</term><term>Enzyme-Linked Immunosorbent Assay (methods)</term><term>Humans</term><term>Nucleocapsid (genetics)</term><term>Nucleocapsid (immunology)</term><term>Polymerase Chain Reaction</term><term>SARS Virus (genetics)</term><term>SARS Virus (immunology)</term><term>SARS Virus (isolation & purification)</term><term>Severe Acute Respiratory Syndrome (diagnosis)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Amorces ADN</term><term>Anticorps antiviraux</term><term>Humains</term><term>Nucléocapside (génétique)</term><term>Nucléocapside (immunologie)</term><term>Réaction de polymérisation en chaîne</term><term>Syndrome respiratoire aigu sévère (diagnostic)</term><term>Séquence nucléotidique</term><term>Test ELISA ()</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (immunologie)</term><term>Virus du SRAS (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Antibodies, Viral</term><term>DNA Primers</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Nucleocapsid</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Nucléocapside</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Nucléocapside</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Nucleocapsid</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Enzyme-Linked Immunosorbent Assay</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Humans</term><term>Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Amorces ADN</term><term>Anticorps antiviraux</term><term>Humains</term><term>Réaction de polymérisation en chaîne</term><term>Séquence nucléotidique</term><term>Test ELISA</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Recombinant severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein was employed to establish an antigen-capturing enzyme-linked immunosorbent assay (ELISA). Antinucleocapsid protein antibodies could be detected in 68.4% of probable SARS patients 6 to 10 days after illness and in 89.6% of the patients 11 to 61 days after illness. No false-positive results were observed in 20 non-SARS fever patients, 24 non-SARS respiratory illness patients, and 20 health care workers. Among 940 other non-SARS clinical serum samples, only 1 was found to be weakly positive. This method provides a new, sensitive, and specific approach for SARS diagnosis.</div></front></TEI><pubmed><MedlineCitation Status="MEDLINE" Owner="NLM"><PMID Version="1">14662982</PMID><DateCompleted><Year>2004</Year><Month>02</Month><Day>27</Day></DateCompleted><DateRevised><Year>2019</Year><Month>05</Month><Day>09</Day></DateRevised><Article PubModel="Print"><Journal><ISSN IssnType="Print">0095-1137</ISSN><JournalIssue CitedMedium="Print"><Volume>41</Volume><Issue>12</Issue><PubDate><Year>2003</Year><Month>Dec</Month></PubDate></JournalIssue><Title>Journal of clinical microbiology</Title><ISOAbbreviation>J. Clin. Microbiol.</ISOAbbreviation></Journal><ArticleTitle>Diagnosis of severe acute respiratory syndrome (SARS) by detection of SARS coronavirus nucleocapsid antibodies in an antigen-capturing enzyme-linked immunosorbent assay.</ArticleTitle><Pagination><MedlinePgn>5781-2</MedlinePgn></Pagination><Abstract><AbstractText>Recombinant severe acute respiratory syndrome (SARS) coronavirus nucleocapsid protein was employed to establish an antigen-capturing enzyme-linked immunosorbent assay (ELISA). Antinucleocapsid protein antibodies could be detected in 68.4% of probable SARS patients 6 to 10 days after illness and in 89.6% of the patients 11 to 61 days after illness. No false-positive results were observed in 20 non-SARS fever patients, 24 non-SARS respiratory illness patients, and 20 health care workers. Among 940 other non-SARS clinical serum samples, only 1 was found to be weakly positive. This method provides a new, sensitive, and specific approach for SARS diagnosis.</AbstractText></Abstract><AuthorList CompleteYN="Y"><Author ValidYN="Y"><LastName>Shi</LastName><ForeName>Yuling</ForeName><Initials>Y</Initials><AffiliationInfo><Affiliation>Beijing Institute of Biotechnology, Beijing 100850, People's Republic of China.</Affiliation></AffiliationInfo></Author><Author ValidYN="Y"><LastName>Yi</LastName><ForeName>Yanping</ForeName><Initials>Y</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Ping</ForeName><Initials>P</Initials></Author><Author ValidYN="Y"><LastName>Kuang</LastName><ForeName>Tieji</ForeName><Initials>T</Initials></Author><Author ValidYN="Y"><LastName>Li</LastName><ForeName>Linhai</ForeName><Initials>L</Initials></Author><Author ValidYN="Y"><LastName>Dong</LastName><ForeName>Mei</ForeName><Initials>M</Initials></Author><Author ValidYN="Y"><LastName>Ma</LastName><ForeName>Qingjun</ForeName><Initials>Q</Initials></Author><Author ValidYN="Y"><LastName>Cao</LastName><ForeName>Cheng</ForeName><Initials>C</Initials></Author></AuthorList><Language>eng</Language><PublicationTypeList><PublicationType UI="D016428">Journal Article</PublicationType></PublicationTypeList></Article><MedlineJournalInfo><Country>United States</Country><MedlineTA>J Clin Microbiol</MedlineTA><NlmUniqueID>7505564</NlmUniqueID><ISSNLinking>0095-1137</ISSNLinking></MedlineJournalInfo><ChemicalList><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D000914">Antibodies, Viral</NameOfSubstance></Chemical><Chemical><RegistryNumber>0</RegistryNumber><NameOfSubstance UI="D017931">DNA Primers</NameOfSubstance></Chemical></ChemicalList><CitationSubset>IM</CitationSubset><MeshHeadingList><MeshHeading><DescriptorName UI="D000914" MajorTopicYN="N">Antibodies, Viral</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D001483" MajorTopicYN="N">Base Sequence</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D017931" MajorTopicYN="N">DNA Primers</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D004797" MajorTopicYN="N">Enzyme-Linked Immunosorbent Assay</DescriptorName><QualifierName UI="Q000379" MajorTopicYN="N">methods</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D006801" MajorTopicYN="N">Humans</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D019251" MajorTopicYN="N">Nucleocapsid</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="Y">immunology</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D016133" MajorTopicYN="N">Polymerase Chain Reaction</DescriptorName></MeshHeading><MeshHeading><DescriptorName UI="D045473" MajorTopicYN="N">SARS Virus</DescriptorName><QualifierName UI="Q000235" MajorTopicYN="N">genetics</QualifierName><QualifierName UI="Q000276" MajorTopicYN="N">immunology</QualifierName><QualifierName UI="Q000302" MajorTopicYN="Y">isolation & purification</QualifierName></MeshHeading><MeshHeading><DescriptorName UI="D045169" MajorTopicYN="N">Severe Acute Respiratory Syndrome</DescriptorName><QualifierName UI="Q000175" MajorTopicYN="Y">diagnosis</QualifierName></MeshHeading></MeshHeadingList></MedlineCitation><PubmedData><History><PubMedPubDate PubStatus="pubmed"><Year>2003</Year><Month>12</Month><Day>10</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="medline"><Year>2004</Year><Month>2</Month><Day>28</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate><PubMedPubDate PubStatus="entrez"><Year>2003</Year><Month>12</Month><Day>10</Day><Hour>5</Hour><Minute>0</Minute></PubMedPubDate></History><PublicationStatus>ppublish</PublicationStatus><ArticleIdList><ArticleId IdType="pubmed">14662982</ArticleId><ArticleId IdType="pmc">PMC309018</ArticleId><ArticleId IdType="doi">10.1128/jcm.41.12.5781-5782.2003</ArticleId></ArticleIdList><ReferenceList><Reference><Citation>N Engl J Med. 2003 May 15;348(20):1953-66</Citation><ArticleIdList><ArticleId IdType="pubmed">12690092</ArticleId></ArticleIdList></Reference><Reference><Citation>Lancet. 2003 Jun 7;361(9373):1984-5</Citation><ArticleIdList><ArticleId IdType="pubmed">12801758</ArticleId></ArticleIdList></Reference><Reference><Citation>Science. 2003 May 30;300(5624):1399-404</Citation><ArticleIdList><ArticleId IdType="pubmed">12730501</ArticleId></ArticleIdList></Reference></ReferenceList></PubmedData></pubmed><affiliations><list><country><li>République populaire de Chine</li></country><settlement><li>Pékin</li></settlement></list><tree><noCountry><name sortKey="Cao, Cheng" sort="Cao, Cheng" uniqKey="Cao C" first="Cheng" last="Cao">Cheng Cao</name><name sortKey="Dong, Mei" sort="Dong, Mei" uniqKey="Dong M" first="Mei" last="Dong">Mei Dong</name><name sortKey="Kuang, Tieji" sort="Kuang, Tieji" uniqKey="Kuang T" first="Tieji" last="Kuang">Tieji Kuang</name><name sortKey="Li, Linhai" sort="Li, Linhai" uniqKey="Li L" first="Linhai" last="Li">Linhai Li</name><name sortKey="Li, Ping" sort="Li, Ping" uniqKey="Li P" first="Ping" last="Li">Ping Li</name><name sortKey="Ma, Qingjun" sort="Ma, Qingjun" uniqKey="Ma Q" first="Qingjun" last="Ma">Qingjun Ma</name><name sortKey="Yi, Yanping" sort="Yi, Yanping" uniqKey="Yi Y" first="Yanping" last="Yi">Yanping Yi</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Shi, Yuling" sort="Shi, Yuling" uniqKey="Shi Y" first="Yuling" last="Shi">Yuling Shi</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Ncbi/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000492 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd -nk 000492 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Ncbi
|étape= Merge
|type= RBID
|clé= pubmed:14662982
|texte= Diagnosis of severe acute respiratory syndrome (SARS) by detection of SARS coronavirus nucleocapsid antibodies in an antigen-capturing enzyme-linked immunosorbent assay.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Merge/RBID.i -Sk "pubmed:14662982" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Merge/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |