The effects of glass surfaces and probe GC content on signal intensities of a 60-mer diagnostic microarray.
Identifieur interne : 003625 ( Ncbi/Curation ); précédent : 003624; suivant : 003626The effects of glass surfaces and probe GC content on signal intensities of a 60-mer diagnostic microarray.
Auteurs : Xiaoyang Mo ; Qinghua Wu [République populaire de Chine] ; Junjian Hu ; Wenli Ma [République populaire de Chine] ; Min Wei [République populaire de Chine] ; Wuzhou Yuan ; Yuequn Wang ; Yongqin Li ; Yun Deng ; Xiushan WuSource :
- Annals of microbiology [ 1590-4261 ] ; 2008.
Abstract
The effects of glass surfaces and probe GC content on signal intensities of a 60-mer diagnostic microarray were studied. Twelve virus-specific oligonucleotide probes for severe acute respiratory syndrome coronavirus (SARS-CoV) were divided into a high GC content group (≥ 50%) and a low GC content group (< 50%), and spotted onto four different chemically-modified glass surfaces: a poly-amine coating activated by 1,4-phenylene diisothiocyanate (Poly-Amine surface), an acrylic acid-co-acrylamide copolymer coating activated by 1-(3-dimethylamino propyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide (AACA-Copolymer surface), a commercial Corning CMT-GAPS amino surface, and a Telechem SuperAmine amino surface. RNA samples from cultured SARS-CoV strain were labelled using direct cDNA labelling with restriction display in a single colour format. The background-subtracted signal intensities were analysed using two-way analysis of variance. The effects of glass surfaces on background-subtracted signal intensities were significant (p=0.003). Multiple comparisons showed that differences existed mainly between the AACA-Copolymer surface and the other glass surfaces, and that the AACA-Copolymer surface had the highest background-subtracted signal intensity. The probe GC content had no significant effect on signal intensities in the narrow range of GC content represented (p=0.07). The results suggested that the AACA-Copolymer surface may be a novel choice of microorganism survey based on long oligonucleotide microarray.
DOI: 10.1007/BF03175336
PubMed: 32226355
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :001C40
- to stream PubMed, to step Curation: Pour aller vers cette notice dans l'étape Curation :001C40
- to stream PubMed, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :001992
- to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :003625
Links to Exploration step
pubmed:32226355Curation
No country items
Xiaoyang Mo<affiliation><nlm:affiliation>1The Center for Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University, 410081 Changsha, Human.</nlm:affiliation>
<wicri:noCountry code="subField">Human</wicri:noCountry>
</affiliation>
<affiliation><nlm:affiliation>1The Center for Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University, 410081 Changsha, Human.</nlm:affiliation>
<wicri:noCountry code="subField">Human</wicri:noCountry>
</affiliation>
<affiliation><nlm:affiliation>1The Center for Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University, 410081 Changsha, Human.</nlm:affiliation>
<wicri:noCountry code="subField">Human</wicri:noCountry>
</affiliation>
<affiliation><nlm:affiliation>1The Center for Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University, 410081 Changsha, Human.</nlm:affiliation>
<wicri:noCountry code="subField">Human</wicri:noCountry>
</affiliation>
<affiliation><nlm:affiliation>1The Center for Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University, 410081 Changsha, Human.</nlm:affiliation>
<wicri:noCountry code="subField">Human</wicri:noCountry>
</affiliation>
<affiliation><nlm:affiliation>1The Center for Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University, 410081 Changsha, Human.</nlm:affiliation>
<wicri:noCountry code="subField">Human</wicri:noCountry>
</affiliation>
<affiliation><nlm:affiliation>1The Center for Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University, 410081 Changsha, Human.</nlm:affiliation>
<wicri:noCountry code="subField">Human</wicri:noCountry>
</affiliation>
Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">The effects of glass surfaces and probe GC content on signal intensities of a 60-mer diagnostic microarray.</title>
<author><name sortKey="Mo, Xiaoyang" sort="Mo, Xiaoyang" uniqKey="Mo X" first="Xiaoyang" last="Mo">Xiaoyang Mo</name>
<affiliation><nlm:affiliation>1The Center for Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University, 410081 Changsha, Human.</nlm:affiliation>
<wicri:noCountry code="subField">Human</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Wu, Qinghua" sort="Wu, Qinghua" uniqKey="Wu Q" first="Qinghua" last="Wu">Qinghua Wu</name>
<affiliation wicri:level="1"><nlm:affiliation>2Key Lab of Biochip, Southern Medical University, 510515 Guangzhou, Guangdong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>2Key Lab of Biochip, Southern Medical University, 510515 Guangzhou, Guangdong</wicri:regionArea>
<wicri:noRegion>Guangdong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hu, Junjian" sort="Hu, Junjian" uniqKey="Hu J" first="Junjian" last="Hu">Junjian Hu</name>
<affiliation><nlm:affiliation>1The Center for Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University, 410081 Changsha, Human.</nlm:affiliation>
<wicri:noCountry code="subField">Human</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Ma, Wenli" sort="Ma, Wenli" uniqKey="Ma W" first="Wenli" last="Ma">Wenli Ma</name>
<affiliation wicri:level="1"><nlm:affiliation>2Key Lab of Biochip, Southern Medical University, 510515 Guangzhou, Guangdong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>2Key Lab of Biochip, Southern Medical University, 510515 Guangzhou, Guangdong</wicri:regionArea>
<wicri:noRegion>Guangdong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wei, Min" sort="Wei, Min" uniqKey="Wei M" first="Min" last="Wei">Min Wei</name>
<affiliation wicri:level="1"><nlm:affiliation>2Key Lab of Biochip, Southern Medical University, 510515 Guangzhou, Guangdong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>2Key Lab of Biochip, Southern Medical University, 510515 Guangzhou, Guangdong</wicri:regionArea>
<wicri:noRegion>Guangdong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Yuan, Wuzhou" sort="Yuan, Wuzhou" uniqKey="Yuan W" first="Wuzhou" last="Yuan">Wuzhou Yuan</name>
<affiliation><nlm:affiliation>1The Center for Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University, 410081 Changsha, Human.</nlm:affiliation>
<wicri:noCountry code="subField">Human</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Wang, Yuequn" sort="Wang, Yuequn" uniqKey="Wang Y" first="Yuequn" last="Wang">Yuequn Wang</name>
<affiliation><nlm:affiliation>1The Center for Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University, 410081 Changsha, Human.</nlm:affiliation>
<wicri:noCountry code="subField">Human</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Li, Yongqin" sort="Li, Yongqin" uniqKey="Li Y" first="Yongqin" last="Li">Yongqin Li</name>
<affiliation><nlm:affiliation>1The Center for Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University, 410081 Changsha, Human.</nlm:affiliation>
<wicri:noCountry code="subField">Human</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Deng, Yun" sort="Deng, Yun" uniqKey="Deng Y" first="Yun" last="Deng">Yun Deng</name>
<affiliation><nlm:affiliation>1The Center for Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University, 410081 Changsha, Human.</nlm:affiliation>
<wicri:noCountry code="subField">Human</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Wu, Xiushan" sort="Wu, Xiushan" uniqKey="Wu X" first="Xiushan" last="Wu">Xiushan Wu</name>
<affiliation><nlm:affiliation>1The Center for Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University, 410081 Changsha, Human.</nlm:affiliation>
<wicri:noCountry code="subField">Human</wicri:noCountry>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2008">2008</date>
<idno type="RBID">pubmed:32226355</idno>
<idno type="pmid">32226355</idno>
<idno type="doi">10.1007/BF03175336</idno>
<idno type="wicri:Area/PubMed/Corpus">001C40</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001C40</idno>
<idno type="wicri:Area/PubMed/Curation">001C40</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001C40</idno>
<idno type="wicri:Area/PubMed/Checkpoint">001992</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001992</idno>
<idno type="wicri:Area/Ncbi/Merge">003625</idno>
<idno type="wicri:Area/Ncbi/Curation">003625</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">The effects of glass surfaces and probe GC content on signal intensities of a 60-mer diagnostic microarray.</title>
<author><name sortKey="Mo, Xiaoyang" sort="Mo, Xiaoyang" uniqKey="Mo X" first="Xiaoyang" last="Mo">Xiaoyang Mo</name>
<affiliation><nlm:affiliation>1The Center for Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University, 410081 Changsha, Human.</nlm:affiliation>
<wicri:noCountry code="subField">Human</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Wu, Qinghua" sort="Wu, Qinghua" uniqKey="Wu Q" first="Qinghua" last="Wu">Qinghua Wu</name>
<affiliation wicri:level="1"><nlm:affiliation>2Key Lab of Biochip, Southern Medical University, 510515 Guangzhou, Guangdong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>2Key Lab of Biochip, Southern Medical University, 510515 Guangzhou, Guangdong</wicri:regionArea>
<wicri:noRegion>Guangdong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Hu, Junjian" sort="Hu, Junjian" uniqKey="Hu J" first="Junjian" last="Hu">Junjian Hu</name>
<affiliation><nlm:affiliation>1The Center for Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University, 410081 Changsha, Human.</nlm:affiliation>
<wicri:noCountry code="subField">Human</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Ma, Wenli" sort="Ma, Wenli" uniqKey="Ma W" first="Wenli" last="Ma">Wenli Ma</name>
<affiliation wicri:level="1"><nlm:affiliation>2Key Lab of Biochip, Southern Medical University, 510515 Guangzhou, Guangdong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>2Key Lab of Biochip, Southern Medical University, 510515 Guangzhou, Guangdong</wicri:regionArea>
<wicri:noRegion>Guangdong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Wei, Min" sort="Wei, Min" uniqKey="Wei M" first="Min" last="Wei">Min Wei</name>
<affiliation wicri:level="1"><nlm:affiliation>2Key Lab of Biochip, Southern Medical University, 510515 Guangzhou, Guangdong, China.</nlm:affiliation>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>2Key Lab of Biochip, Southern Medical University, 510515 Guangzhou, Guangdong</wicri:regionArea>
<wicri:noRegion>Guangdong</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Yuan, Wuzhou" sort="Yuan, Wuzhou" uniqKey="Yuan W" first="Wuzhou" last="Yuan">Wuzhou Yuan</name>
<affiliation><nlm:affiliation>1The Center for Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University, 410081 Changsha, Human.</nlm:affiliation>
<wicri:noCountry code="subField">Human</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Wang, Yuequn" sort="Wang, Yuequn" uniqKey="Wang Y" first="Yuequn" last="Wang">Yuequn Wang</name>
<affiliation><nlm:affiliation>1The Center for Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University, 410081 Changsha, Human.</nlm:affiliation>
<wicri:noCountry code="subField">Human</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Li, Yongqin" sort="Li, Yongqin" uniqKey="Li Y" first="Yongqin" last="Li">Yongqin Li</name>
<affiliation><nlm:affiliation>1The Center for Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University, 410081 Changsha, Human.</nlm:affiliation>
<wicri:noCountry code="subField">Human</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Deng, Yun" sort="Deng, Yun" uniqKey="Deng Y" first="Yun" last="Deng">Yun Deng</name>
<affiliation><nlm:affiliation>1The Center for Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University, 410081 Changsha, Human.</nlm:affiliation>
<wicri:noCountry code="subField">Human</wicri:noCountry>
</affiliation>
</author>
<author><name sortKey="Wu, Xiushan" sort="Wu, Xiushan" uniqKey="Wu X" first="Xiushan" last="Wu">Xiushan Wu</name>
<affiliation><nlm:affiliation>1The Center for Heart Development, Key Lab of MOE for Development Biology and Protein Chemistry, College of Life Sciences, Hunan Normal University, 410081 Changsha, Human.</nlm:affiliation>
<wicri:noCountry code="subField">Human</wicri:noCountry>
</affiliation>
</author>
</analytic>
<series><title level="j">Annals of microbiology</title>
<idno type="ISSN">1590-4261</idno>
<imprint><date when="2008" type="published">2008</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass></textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">The effects of glass surfaces and probe GC content on signal intensities of a 60-mer diagnostic microarray were studied. Twelve virus-specific oligonucleotide probes for severe acute respiratory syndrome coronavirus (SARS-CoV) were divided into a high GC content group (≥ 50%) and a low GC content group (< 50%), and spotted onto four different chemically-modified glass surfaces: a poly-amine coating activated by 1,4-phenylene diisothiocyanate (Poly-Amine surface), an acrylic acid-co-acrylamide copolymer coating activated by 1-(3-dimethylamino propyl)-3-ethylcarbodiimide hydrochloride and N-hydroxysuccinimide (AACA-Copolymer surface), a commercial Corning CMT-GAPS amino surface, and a Telechem SuperAmine amino surface. RNA samples from cultured SARS-CoV strain were labelled using direct cDNA labelling with restriction display in a single colour format. The background-subtracted signal intensities were analysed using two-way analysis of variance. The effects of glass surfaces on background-subtracted signal intensities were significant (p=0.003). Multiple comparisons showed that differences existed mainly between the AACA-Copolymer surface and the other glass surfaces, and that the AACA-Copolymer surface had the highest background-subtracted signal intensity. The probe GC content had no significant effect on signal intensities in the narrow range of GC content represented (p=0.07). The results suggested that the AACA-Copolymer surface may be a novel choice of microorganism survey based on long oligonucleotide microarray.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Ncbi/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 003625 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd -nk 003625 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= SrasV1 |flux= Ncbi |étape= Curation |type= RBID |clé= pubmed:32226355 |texte= The effects of glass surfaces and probe GC content on signal intensities of a 60-mer diagnostic microarray. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/RBID.i -Sk "pubmed:32226355" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd \ | NlmPubMed2Wicri -a SrasV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |