SrasV1, Ncbi, Curation, bibRecord, 002508

Application of purified recombinant antigenic spike fragments to the diagnosis of avian infectious bronchitis virus infection.

Identifieur interne : 002508 ( Ncbi/Curation ); précédent : 002507; suivant : 002509

Application of purified recombinant antigenic spike fragments to the diagnosis of avian infectious bronchitis virus infection.

Auteurs : Kuan-Hsun Lin [Taïwan] ; Chuen-Fu Lin ; Shyan-Song Chiou ; Ai-Ping Hsu ; Min-Shiuh Lee ; Chao-Chin Chang ; Tien-Jye Chang ; Jui-Hung Shien ; Wei-Li Hsu

Source :

Applied microbiology and biotechnology [ 1432-0614 ] ; 2012.

RBID : pubmed:22627759

Descripteurs français

English descriptors

KwdEn :
- Amino Acid Sequence, Animals, Antibodies, Viral (blood), Antigens, Viral (genetics), Antigens, Viral (immunology), Antigens, Viral (isolation & purification), Antigens, Viral (metabolism), Chickens, Coronavirus Infections (diagnosis), Coronavirus Infections (immunology), Coronavirus Infections (veterinary), Coronavirus Infections (virology), Enzyme-Linked Immunosorbent Assay, Infectious bronchitis virus (genetics), Infectious bronchitis virus (immunology), Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Membrane Glycoproteins (isolation & purification), Membrane Glycoproteins (metabolism), Molecular Sequence Data, Poultry Diseases (diagnosis), Poultry Diseases (immunology), Poultry Diseases (virology), Recombinant Proteins (genetics), Recombinant Proteins (immunology), Recombinant Proteins (isolation & purification), Recombinant Proteins (metabolism), Sensitivity and Specificity, Sequence Analysis, DNA, Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology), Viral Envelope Proteins (isolation & purification), Viral Envelope Proteins (metabolism).
MESH :
- chemical , blood : Antibodies, Viral.
- chemical , genetics : Antigens, Viral, Membrane Glycoproteins, Recombinant Proteins, Viral Envelope Proteins.
- chemical , immunology : Antigens, Viral, Membrane Glycoproteins, Recombinant Proteins, Viral Envelope Proteins.
- chemical , isolation & purification : Antigens, Viral, Membrane Glycoproteins, Recombinant Proteins, Viral Envelope Proteins.
- chemical , metabolism : Antigens, Viral, Membrane Glycoproteins, Recombinant Proteins, Viral Envelope Proteins.
- diagnosis : Coronavirus Infections, Poultry Diseases.
- genetics : Infectious bronchitis virus.
- immunology : Coronavirus Infections, Infectious bronchitis virus, Poultry Diseases.
- veterinary : Coronavirus Infections.
- virology : Coronavirus Infections, Poultry Diseases.
- Amino Acid Sequence, Animals, Chickens, Enzyme-Linked Immunosorbent Assay, Molecular Sequence Data, Sensitivity and Specificity, Sequence Analysis, DNA, Spike Glycoprotein, Coronavirus.

Abstract

The spike (S) protein, containing two subunits, S1 and S2, is the major immunity-eliciting antigen of avian infectious bronchitis virus (IBV), a highly contagious disease of chickens. Several immunogenic regions, mainly located within the S1 subunit, have been identified. Nonetheless, these immune-dominant regions were defined using selected monoclonal antibodies or using a short peptide approach that involves only certain limited regions of the S protein. In addition, some immune-dominant regions are located in hypervariable regions (HVRs) which are not present in all serotypes. Hence, the aim of this study was to determine a broader range of antigenic regions that have strong antibody eliciting ability; these could then be applied for development of an IBV-diagnostic tool. Initially, the S1 and part of the S2 subunit protein (24-567 amino acids) were expressed as five fragments in prokaryotic system. The antigenicity was confirmed using IBV immunized sera. Performance of the S subfragments was evaluated by ELISA using a panel of field chicken sera with known IBV titres determined by a commercial kit. This indicated that, among the five antigenic recombinant proteins, the region S-E showed the highest specificity and sensitivity, namely 95.38 % and 96.29 %, respectively. The κ value for the in-house ELISA using the S-E fragment compared to a commercial kit was 0.9172, indicating a high agreement between these two methods. As region S-E harbors strong immunogenicity within the spike protein, it has the potential to be exploited as an antigen when developing a cost-effective ELISA-based diagnosis tool.

DOI: 10.1007/s00253-012-4143-8
PubMed: 22627759

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pubmed:22627759

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<author><name sortKey="Lin, Kuan Hsun" sort="Lin, Kuan Hsun" uniqKey="Lin K" first="Kuan-Hsun" last="Lin">Kuan-Hsun Lin</name>
<affiliation wicri:level="1"><nlm:affiliation>Department of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 402, Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Department of Veterinary Medicine, College of Veterinary Medicine, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 402</wicri:regionArea>
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<author><name sortKey="Lin, Chuen Fu" sort="Lin, Chuen Fu" uniqKey="Lin C" first="Chuen-Fu" last="Lin">Chuen-Fu Lin</name>
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<author><name sortKey="Chiou, Shyan Song" sort="Chiou, Shyan Song" uniqKey="Chiou S" first="Shyan-Song" last="Chiou">Shyan-Song Chiou</name>
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<author><name sortKey="Hsu, Ai Ping" sort="Hsu, Ai Ping" uniqKey="Hsu A" first="Ai-Ping" last="Hsu">Ai-Ping Hsu</name>
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<author><name sortKey="Lee, Min Shiuh" sort="Lee, Min Shiuh" uniqKey="Lee M" first="Min-Shiuh" last="Lee">Min-Shiuh Lee</name>
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<author><name sortKey="Chang, Chao Chin" sort="Chang, Chao Chin" uniqKey="Chang C" first="Chao-Chin" last="Chang">Chao-Chin Chang</name>
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<author><name sortKey="Chang, Tien Jye" sort="Chang, Tien Jye" uniqKey="Chang T" first="Tien-Jye" last="Chang">Tien-Jye Chang</name>
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<author><name sortKey="Shien, Jui Hung" sort="Shien, Jui Hung" uniqKey="Shien J" first="Jui-Hung" last="Shien">Jui-Hung Shien</name>
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<author><name sortKey="Hsu, Wei Li" sort="Hsu, Wei Li" uniqKey="Hsu W" first="Wei-Li" last="Hsu">Wei-Li Hsu</name>
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<author><name sortKey="Lee, Min Shiuh" sort="Lee, Min Shiuh" uniqKey="Lee M" first="Min-Shiuh" last="Lee">Min-Shiuh Lee</name>
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<author><name sortKey="Chang, Tien Jye" sort="Chang, Tien Jye" uniqKey="Chang T" first="Tien-Jye" last="Chang">Tien-Jye Chang</name>
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<author><name sortKey="Shien, Jui Hung" sort="Shien, Jui Hung" uniqKey="Shien J" first="Jui-Hung" last="Shien">Jui-Hung Shien</name>
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<author><name sortKey="Hsu, Wei Li" sort="Hsu, Wei Li" uniqKey="Hsu W" first="Wei-Li" last="Hsu">Wei-Li Hsu</name>
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<series><title level="j">Applied microbiology and biotechnology</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Antibodies, Viral (blood)</term>
<term>Antigens, Viral (genetics)</term>
<term>Antigens, Viral (immunology)</term>
<term>Antigens, Viral (isolation & purification)</term>
<term>Antigens, Viral (metabolism)</term>
<term>Chickens</term>
<term>Coronavirus Infections (diagnosis)</term>
<term>Coronavirus Infections (immunology)</term>
<term>Coronavirus Infections (veterinary)</term>
<term>Coronavirus Infections (virology)</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Infectious bronchitis virus (genetics)</term>
<term>Infectious bronchitis virus (immunology)</term>
<term>Membrane Glycoproteins (genetics)</term>
<term>Membrane Glycoproteins (immunology)</term>
<term>Membrane Glycoproteins (isolation & purification)</term>
<term>Membrane Glycoproteins (metabolism)</term>
<term>Molecular Sequence Data</term>
<term>Poultry Diseases (diagnosis)</term>
<term>Poultry Diseases (immunology)</term>
<term>Poultry Diseases (virology)</term>
<term>Recombinant Proteins (genetics)</term>
<term>Recombinant Proteins (immunology)</term>
<term>Recombinant Proteins (isolation & purification)</term>
<term>Recombinant Proteins (metabolism)</term>
<term>Sensitivity and Specificity</term>
<term>Sequence Analysis, DNA</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Viral Envelope Proteins (genetics)</term>
<term>Viral Envelope Proteins (immunology)</term>
<term>Viral Envelope Proteins (isolation & purification)</term>
<term>Viral Envelope Proteins (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de séquence d'ADN</term>
<term>Animaux</term>
<term>Anticorps antiviraux (sang)</term>
<term>Antigènes viraux (génétique)</term>
<term>Antigènes viraux (immunologie)</term>
<term>Antigènes viraux (isolement et purification)</term>
<term>Antigènes viraux (métabolisme)</term>
<term>Données de séquences moléculaires</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires (génétique)</term>
<term>Glycoprotéines membranaires (immunologie)</term>
<term>Glycoprotéines membranaires (isolement et purification)</term>
<term>Glycoprotéines membranaires (métabolisme)</term>
<term>Infections à coronavirus (diagnostic)</term>
<term>Infections à coronavirus (immunologie)</term>
<term>Infections à coronavirus (médecine vétérinaire)</term>
<term>Infections à coronavirus (virologie)</term>
<term>Maladies de la volaille (diagnostic)</term>
<term>Maladies de la volaille (immunologie)</term>
<term>Maladies de la volaille (virologie)</term>
<term>Poulets</term>
<term>Protéines de l'enveloppe virale (génétique)</term>
<term>Protéines de l'enveloppe virale (immunologie)</term>
<term>Protéines de l'enveloppe virale (isolement et purification)</term>
<term>Protéines de l'enveloppe virale (métabolisme)</term>
<term>Protéines recombinantes (génétique)</term>
<term>Protéines recombinantes (immunologie)</term>
<term>Protéines recombinantes (isolement et purification)</term>
<term>Protéines recombinantes (métabolisme)</term>
<term>Sensibilité et spécificité</term>
<term>Séquence d'acides aminés</term>
<term>Test ELISA</term>
<term>Virus de la bronchite infectieuse (génétique)</term>
<term>Virus de la bronchite infectieuse (immunologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Antigens, Viral</term>
<term>Membrane Glycoproteins</term>
<term>Recombinant Proteins</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antigens, Viral</term>
<term>Membrane Glycoproteins</term>
<term>Recombinant Proteins</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>Antigens, Viral</term>
<term>Membrane Glycoproteins</term>
<term>Recombinant Proteins</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Antigens, Viral</term>
<term>Membrane Glycoproteins</term>
<term>Recombinant Proteins</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Coronavirus Infections</term>
<term>Poultry Diseases</term>
</keywords>
<keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Infections à coronavirus</term>
<term>Maladies de la volaille</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Infectious bronchitis virus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Antigènes viraux</term>
<term>Glycoprotéines membranaires</term>
<term>Protéines de l'enveloppe virale</term>
<term>Protéines recombinantes</term>
<term>Virus de la bronchite infectieuse</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Antigènes viraux</term>
<term>Glycoprotéines membranaires</term>
<term>Infections à coronavirus</term>
<term>Maladies de la volaille</term>
<term>Protéines de l'enveloppe virale</term>
<term>Protéines recombinantes</term>
<term>Virus de la bronchite infectieuse</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Coronavirus Infections</term>
<term>Infectious bronchitis virus</term>
<term>Poultry Diseases</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Antigènes viraux</term>
<term>Glycoprotéines membranaires</term>
<term>Protéines de l'enveloppe virale</term>
<term>Protéines recombinantes</term>
</keywords>
<keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Infections à coronavirus</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Antigènes viraux</term>
<term>Glycoprotéines membranaires</term>
<term>Protéines de l'enveloppe virale</term>
<term>Protéines recombinantes</term>
</keywords>
<keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term>
</keywords>
<keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Coronavirus Infections</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Infections à coronavirus</term>
<term>Maladies de la volaille</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Coronavirus Infections</term>
<term>Poultry Diseases</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Chickens</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Molecular Sequence Data</term>
<term>Sensitivity and Specificity</term>
<term>Sequence Analysis, DNA</term>
<term>Spike Glycoprotein, Coronavirus</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term>
<term>Animaux</term>
<term>Données de séquences moléculaires</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Poulets</term>
<term>Sensibilité et spécificité</term>
<term>Séquence d'acides aminés</term>
<term>Test ELISA</term>
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<front><div type="abstract" xml:lang="en">The spike (S) protein, containing two subunits, S1 and S2, is the major immunity-eliciting antigen of avian infectious bronchitis virus (IBV), a highly contagious disease of chickens. Several immunogenic regions, mainly located within the S1 subunit, have been identified. Nonetheless, these immune-dominant regions were defined using selected monoclonal antibodies or using a short peptide approach that involves only certain limited regions of the S protein. In addition, some immune-dominant regions are located in hypervariable regions (HVRs) which are not present in all serotypes. Hence, the aim of this study was to determine a broader range of antigenic regions that have strong antibody eliciting ability; these could then be applied for development of an IBV-diagnostic tool. Initially, the S1 and part of the S2 subunit protein (24-567 amino acids) were expressed as five fragments in prokaryotic system. The antigenicity was confirmed using IBV immunized sera. Performance of the S subfragments was evaluated by ELISA using a panel of field chicken sera with known IBV titres determined by a commercial kit. This indicated that, among the five antigenic recombinant proteins, the region S-E showed the highest specificity and sensitivity, namely 95.38 % and 96.29 %, respectively. The κ value for the in-house ELISA using the S-E fragment compared to a commercial kit was 0.9172, indicating a high agreement between these two methods. As region S-E harbors strong immunogenicity within the spike protein, it has the potential to be exploited as an antigen when developing a cost-effective ELISA-based diagnosis tool.</div>
</front>
</TEI>
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Application of purified recombinant antigenic spike fragments to the diagnosis of avian infectious bronchitis virus infection.

Application of purified recombinant antigenic spike fragments to the diagnosis of avian infectious bronchitis virus infection.

Source :

Descripteurs français

English descriptors

Abstract

Links toward previous steps (curation, corpus...)

Links to Exploration step

Le document en format XML

Pour manipuler ce document sous Unix (Dilib)

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Pour générer des pages wiki