Immunization with SARS Coronavirus Vaccines Leads to Pulmonary Immunopathology on Challenge with the SARS Virus
Identifieur interne : 002490 ( Ncbi/Curation ); précédent : 002489; suivant : 002491Immunization with SARS Coronavirus Vaccines Leads to Pulmonary Immunopathology on Challenge with the SARS Virus
Auteurs : Chien-Te Tseng [États-Unis] ; Elena Sbrana [États-Unis] ; Naoko Iwata-Yoshikawa [États-Unis] ; Patrick C. Newman [États-Unis] ; Tania Garron [États-Unis] ; Robert L. Atmar [États-Unis] ; Clarence J. Peters [États-Unis] ; Robert B. Couch [États-Unis]Source :
- PLoS ONE [ 1932-6203 ] ; 2012.
Descripteurs français
- KwdFr :
- Animaux, Cellules Vero, Femelle, Granulocytes éosinophiles (immunologie), Lymphocytes auxiliaires Th2 (immunologie), Poumon (anatomopathologie), Poumon (virologie), Protéines de l'enveloppe virale (immunologie), Souris, Souris de lignée BALB C, Souris de lignée C57BL, Syndrome respiratoire aigu sévère (), Syndrome respiratoire aigu sévère (virologie), Techniques de culture de tissus, Vaccination (effets indésirables), Vaccins antiviraux (effets indésirables), Vaccins antiviraux (immunologie), Vaccins inactivés (effets indésirables), Vaccins inactivés (immunologie), Virus du SRAS (immunologie).
- MESH :
- anatomopathologie : Poumon.
- effets indésirables : Vaccination, Vaccins antiviraux, Vaccins inactivés.
- immunologie : Granulocytes éosinophiles, Lymphocytes auxiliaires Th2, Protéines de l'enveloppe virale, Vaccins antiviraux, Vaccins inactivés, Virus du SRAS.
- virologie : Poumon, Syndrome respiratoire aigu sévère.
- Animaux, Cellules Vero, Femelle, Souris, Souris de lignée BALB C, Souris de lignée C57BL, Syndrome respiratoire aigu sévère, Techniques de culture de tissus.
English descriptors
- KwdEn :
- Animals, Chlorocebus aethiops, Eosinophils (immunology), Female, Lung (pathology), Lung (virology), Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, SARS Virus (immunology), Severe Acute Respiratory Syndrome (prevention & control), Severe Acute Respiratory Syndrome (virology), Th2 Cells (immunology), Tissue Culture Techniques, Vaccination (adverse effects), Vaccines, Inactivated (adverse effects), Vaccines, Inactivated (immunology), Vero Cells, Viral Envelope Proteins (immunology), Viral Vaccines (adverse effects), Viral Vaccines (immunology).
- MESH :
- chemical , adverse effects : Vaccines, Inactivated, Viral Vaccines.
- adverse effects : Vaccination.
- immunology : Eosinophils, SARS Virus, Th2 Cells, Vaccines, Inactivated, Viral Envelope Proteins, Viral Vaccines.
- pathology : Lung.
- prevention & control : Severe Acute Respiratory Syndrome.
- virology : Lung, Severe Acute Respiratory Syndrome.
- Animals, Chlorocebus aethiops, Female, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Tissue Culture Techniques, Vero Cells.
Abstract
Severe acute respiratory syndrome (SARS) emerged in China in 2002 and spread to other countries before brought under control. Because of a concern for reemergence or a deliberate release of the SARS coronavirus, vaccine development was initiated. Evaluations of an inactivated whole virus vaccine in ferrets and nonhuman primates and a virus-like-particle vaccine in mice induced protection against infection but challenged animals exhibited an immunopathologic-type lung disease.
Four candidate vaccines for humans with or without alum adjuvant were evaluated in a mouse model of SARS, a VLP vaccine, the vaccine given to ferrets and NHP, another whole virus vaccine and an rDNA-produced S protein. Balb/c or C57BL/6 mice were vaccinated IM on day 0 and 28 and sacrificed for serum antibody measurements or challenged with live virus on day 56. On day 58, challenged mice were sacrificed and lungs obtained for virus and histopathology.
All vaccines induced serum neutralizing antibody with increasing dosages and/or alum significantly increasing responses. Significant reductions of SARS-CoV two days after challenge was seen for all vaccines and prior live SARS-CoV. All mice exhibited histopathologic changes in lungs two days after challenge including all animals vaccinated (Balb/C and C57BL/6) or given live virus, influenza vaccine, or PBS suggesting infection occurred in all. Histopathology seen in animals given one of the SARS-CoV vaccines was uniformly a Th2-type immunopathology with prominent eosinophil infiltration, confirmed with special eosinophil stains. The pathologic changes seen in all control groups lacked the eosinophil prominence.
These SARS-CoV vaccines all induced antibody and protection against infection with SARS-CoV. However, challenge of mice given any of the vaccines led to occurrence of Th2-type immunopathology suggesting hypersensitivity to SARS-CoV components was induced. Caution in proceeding to application of a SARS-CoV vaccine in humans is indicated.
Url:
DOI: 10.1371/journal.pone.0035421
PubMed: 22536382
PubMed Central: 3335060
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :001377
- to stream Pmc, to step Curation: Pour aller vers cette notice dans l'étape Curation :001377
- to stream Pmc, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :000A56
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :001372
- to stream PubMed, to step Curation: Pour aller vers cette notice dans l'étape Curation :001372
- to stream PubMed, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :001339
- to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :002490
Links to Exploration step
PMC:3335060Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Immunization with SARS Coronavirus Vaccines Leads to Pulmonary Immunopathology on Challenge with the SARS Virus</title><author><name sortKey="Tseng, Chien Te" sort="Tseng, Chien Te" uniqKey="Tseng C" first="Chien-Te" last="Tseng">Chien-Te Tseng</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, Texas, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, Texas</wicri:regionArea><placeName><region type="state">Texas</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="aff2"><addr-line>Center for Biodefense and Emerging Disease, The University of Texas Medical Branch, Galveston, Texas, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Biodefense and Emerging Disease, The University of Texas Medical Branch, Galveston, Texas</wicri:regionArea><placeName><region type="state">Texas</region></placeName></affiliation></author><author><name sortKey="Sbrana, Elena" sort="Sbrana, Elena" uniqKey="Sbrana E" first="Elena" last="Sbrana">Elena Sbrana</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, Texas, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, Texas</wicri:regionArea><placeName><region type="state">Texas</region></placeName></affiliation></author><author><name sortKey="Iwata Yoshikawa, Naoko" sort="Iwata Yoshikawa, Naoko" uniqKey="Iwata Yoshikawa N" first="Naoko" last="Iwata-Yoshikawa">Naoko Iwata-Yoshikawa</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, Texas, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, Texas</wicri:regionArea><placeName><region type="state">Texas</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="aff2"><addr-line>Center for Biodefense and Emerging Disease, The University of Texas Medical Branch, Galveston, Texas, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Biodefense and Emerging Disease, The University of Texas Medical Branch, Galveston, Texas</wicri:regionArea><placeName><region type="state">Texas</region></placeName></affiliation></author><author><name sortKey="Newman, Patrick C" sort="Newman, Patrick C" uniqKey="Newman P" first="Patrick C." last="Newman">Patrick C. Newman</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, Texas, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, Texas</wicri:regionArea><placeName><region type="state">Texas</region></placeName></affiliation></author><author><name sortKey="Garron, Tania" sort="Garron, Tania" uniqKey="Garron T" first="Tania" last="Garron">Tania Garron</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, Texas, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, Texas</wicri:regionArea><placeName><region type="state">Texas</region></placeName></affiliation></author><author><name sortKey="Atmar, Robert L" sort="Atmar, Robert L" uniqKey="Atmar R" first="Robert L." last="Atmar">Robert L. Atmar</name><affiliation wicri:level="2"><nlm:aff id="aff3"><addr-line>Department of Medicine, Baylor College of Medicine, Houston, Texas, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Medicine, Baylor College of Medicine, Houston, Texas</wicri:regionArea><placeName><region type="state">Texas</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="aff4"><addr-line>Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas</wicri:regionArea><placeName><region type="state">Texas</region></placeName></affiliation></author><author><name sortKey="Peters, Clarence J" sort="Peters, Clarence J" uniqKey="Peters C" first="Clarence J." last="Peters">Clarence J. Peters</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, Texas, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, Texas</wicri:regionArea><placeName><region type="state">Texas</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="aff2"><addr-line>Center for Biodefense and Emerging Disease, The University of Texas Medical Branch, Galveston, Texas, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Biodefense and Emerging Disease, The University of Texas Medical Branch, Galveston, Texas</wicri:regionArea><placeName><region type="state">Texas</region></placeName></affiliation></author><author><name sortKey="Couch, Robert B" sort="Couch, Robert B" uniqKey="Couch R" first="Robert B." last="Couch">Robert B. Couch</name><affiliation wicri:level="2"><nlm:aff id="aff3"><addr-line>Department of Medicine, Baylor College of Medicine, Houston, Texas, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Medicine, Baylor College of Medicine, Houston, Texas</wicri:regionArea><placeName><region type="state">Texas</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="aff4"><addr-line>Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas</wicri:regionArea><placeName><region type="state">Texas</region></placeName></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">22536382</idno><idno type="pmc">3335060</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC3335060</idno><idno type="RBID">PMC:3335060</idno><idno type="doi">10.1371/journal.pone.0035421</idno><date when="2012">2012</date><idno type="wicri:Area/Pmc/Corpus">001377</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001377</idno><idno type="wicri:Area/Pmc/Curation">001377</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">001377</idno><idno type="wicri:Area/Pmc/Checkpoint">000A56</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000A56</idno><idno type="wicri:source">PubMed</idno><idno type="RBID">pubmed:22536382</idno><idno type="wicri:Area/PubMed/Corpus">001372</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001372</idno><idno type="wicri:Area/PubMed/Curation">001372</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001372</idno><idno type="wicri:Area/PubMed/Checkpoint">001339</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001339</idno><idno type="wicri:Area/Ncbi/Merge">002490</idno><idno type="wicri:Area/Ncbi/Curation">002490</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Immunization with SARS Coronavirus Vaccines Leads to Pulmonary Immunopathology on Challenge with the SARS Virus</title><author><name sortKey="Tseng, Chien Te" sort="Tseng, Chien Te" uniqKey="Tseng C" first="Chien-Te" last="Tseng">Chien-Te Tseng</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, Texas, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, Texas</wicri:regionArea><placeName><region type="state">Texas</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="aff2"><addr-line>Center for Biodefense and Emerging Disease, The University of Texas Medical Branch, Galveston, Texas, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Biodefense and Emerging Disease, The University of Texas Medical Branch, Galveston, Texas</wicri:regionArea><placeName><region type="state">Texas</region></placeName></affiliation></author><author><name sortKey="Sbrana, Elena" sort="Sbrana, Elena" uniqKey="Sbrana E" first="Elena" last="Sbrana">Elena Sbrana</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, Texas, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, Texas</wicri:regionArea><placeName><region type="state">Texas</region></placeName></affiliation></author><author><name sortKey="Iwata Yoshikawa, Naoko" sort="Iwata Yoshikawa, Naoko" uniqKey="Iwata Yoshikawa N" first="Naoko" last="Iwata-Yoshikawa">Naoko Iwata-Yoshikawa</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, Texas, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, Texas</wicri:regionArea><placeName><region type="state">Texas</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="aff2"><addr-line>Center for Biodefense and Emerging Disease, The University of Texas Medical Branch, Galveston, Texas, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Biodefense and Emerging Disease, The University of Texas Medical Branch, Galveston, Texas</wicri:regionArea><placeName><region type="state">Texas</region></placeName></affiliation></author><author><name sortKey="Newman, Patrick C" sort="Newman, Patrick C" uniqKey="Newman P" first="Patrick C." last="Newman">Patrick C. Newman</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, Texas, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, Texas</wicri:regionArea><placeName><region type="state">Texas</region></placeName></affiliation></author><author><name sortKey="Garron, Tania" sort="Garron, Tania" uniqKey="Garron T" first="Tania" last="Garron">Tania Garron</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, Texas, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, Texas</wicri:regionArea><placeName><region type="state">Texas</region></placeName></affiliation></author><author><name sortKey="Atmar, Robert L" sort="Atmar, Robert L" uniqKey="Atmar R" first="Robert L." last="Atmar">Robert L. Atmar</name><affiliation wicri:level="2"><nlm:aff id="aff3"><addr-line>Department of Medicine, Baylor College of Medicine, Houston, Texas, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Medicine, Baylor College of Medicine, Houston, Texas</wicri:regionArea><placeName><region type="state">Texas</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="aff4"><addr-line>Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas</wicri:regionArea><placeName><region type="state">Texas</region></placeName></affiliation></author><author><name sortKey="Peters, Clarence J" sort="Peters, Clarence J" uniqKey="Peters C" first="Clarence J." last="Peters">Clarence J. Peters</name><affiliation wicri:level="2"><nlm:aff id="aff1"><addr-line>Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, Texas, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Microbiology and Immunology, The University of Texas Medical Branch, Galveston, Texas</wicri:regionArea><placeName><region type="state">Texas</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="aff2"><addr-line>Center for Biodefense and Emerging Disease, The University of Texas Medical Branch, Galveston, Texas, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Center for Biodefense and Emerging Disease, The University of Texas Medical Branch, Galveston, Texas</wicri:regionArea><placeName><region type="state">Texas</region></placeName></affiliation></author><author><name sortKey="Couch, Robert B" sort="Couch, Robert B" uniqKey="Couch R" first="Robert B." last="Couch">Robert B. Couch</name><affiliation wicri:level="2"><nlm:aff id="aff3"><addr-line>Department of Medicine, Baylor College of Medicine, Houston, Texas, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Medicine, Baylor College of Medicine, Houston, Texas</wicri:regionArea><placeName><region type="state">Texas</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="aff4"><addr-line>Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas, United States of America</addr-line></nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Department of Molecular Virology and Microbiology, Baylor College of Medicine, Houston, Texas</wicri:regionArea><placeName><region type="state">Texas</region></placeName></affiliation></author></analytic><series><title level="j">PLoS ONE</title><idno type="eISSN">1932-6203</idno><imprint><date when="2012">2012</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Eosinophils (immunology)</term><term>Female</term><term>Lung (pathology)</term><term>Lung (virology)</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Mice, Inbred C57BL</term><term>SARS Virus (immunology)</term><term>Severe Acute Respiratory Syndrome (prevention & control)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>Th2 Cells (immunology)</term><term>Tissue Culture Techniques</term><term>Vaccination (adverse effects)</term><term>Vaccines, Inactivated (adverse effects)</term><term>Vaccines, Inactivated (immunology)</term><term>Vero Cells</term><term>Viral Envelope Proteins (immunology)</term><term>Viral Vaccines (adverse effects)</term><term>Viral Vaccines (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Femelle</term><term>Granulocytes éosinophiles (immunologie)</term><term>Lymphocytes auxiliaires Th2 (immunologie)</term><term>Poumon (anatomopathologie)</term><term>Poumon (virologie)</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Souris de lignée C57BL</term><term>Syndrome respiratoire aigu sévère ()</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Techniques de culture de tissus</term><term>Vaccination (effets indésirables)</term><term>Vaccins antiviraux (effets indésirables)</term><term>Vaccins antiviraux (immunologie)</term><term>Vaccins inactivés (effets indésirables)</term><term>Vaccins inactivés (immunologie)</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="adverse effects" xml:lang="en"><term>Vaccines, Inactivated</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" qualifier="adverse effects" xml:lang="en"><term>Vaccination</term></keywords><keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Poumon</term></keywords><keywords scheme="MESH" qualifier="effets indésirables" xml:lang="fr"><term>Vaccination</term><term>Vaccins antiviraux</term><term>Vaccins inactivés</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Granulocytes éosinophiles</term><term>Lymphocytes auxiliaires Th2</term><term>Protéines de l'enveloppe virale</term><term>Vaccins antiviraux</term><term>Vaccins inactivés</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Eosinophils</term><term>SARS Virus</term><term>Th2 Cells</term><term>Vaccines, Inactivated</term><term>Viral Envelope Proteins</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Lung</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Poumon</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Lung</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Chlorocebus aethiops</term><term>Female</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Mice, Inbred C57BL</term><term>Tissue Culture Techniques</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Femelle</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Souris de lignée C57BL</term><term>Syndrome respiratoire aigu sévère</term><term>Techniques de culture de tissus</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><sec><title>Background</title><p>Severe acute respiratory syndrome (SARS) emerged in China in 2002 and spread to other countries before brought under control. Because of a concern for reemergence or a deliberate release of the SARS coronavirus, vaccine development was initiated. Evaluations of an inactivated whole virus vaccine in ferrets and nonhuman primates and a virus-like-particle vaccine in mice induced protection against infection but challenged animals exhibited an immunopathologic-type lung disease.</p></sec><sec><title>Design</title><p>Four candidate vaccines for humans with or without alum adjuvant were evaluated in a mouse model of SARS, a VLP vaccine, the vaccine given to ferrets and NHP, another whole virus vaccine and an rDNA-produced S protein. Balb/c or C57BL/6 mice were vaccinated IM on day 0 and 28 and sacrificed for serum antibody measurements or challenged with live virus on day 56. On day 58, challenged mice were sacrificed and lungs obtained for virus and histopathology.</p></sec><sec><title>Results</title><p>All vaccines induced serum neutralizing antibody with increasing dosages and/or alum significantly increasing responses. Significant reductions of SARS-CoV two days after challenge was seen for all vaccines and prior live SARS-CoV. All mice exhibited histopathologic changes in lungs two days after challenge including all animals vaccinated (Balb/C and C57BL/6) or given live virus, influenza vaccine, or PBS suggesting infection occurred in all. Histopathology seen in animals given one of the SARS-CoV vaccines was uniformly a Th2-type immunopathology with prominent eosinophil infiltration, confirmed with special eosinophil stains. The pathologic changes seen in all control groups lacked the eosinophil prominence.</p></sec><sec><title>Conclusions</title><p>These SARS-CoV vaccines all induced antibody and protection against infection with SARS-CoV. However, challenge of mice given any of the vaccines led to occurrence of Th2-type immunopathology suggesting hypersensitivity to SARS-CoV components was induced. Caution in proceeding to application of a SARS-CoV vaccine in humans is indicated.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Tsang, Kw" uniqKey="Tsang K">KW Tsang</name></author><author><name sortKey="Ho, Pl" uniqKey="Ho P">PL Ho</name></author><author><name sortKey="Ooi, Cg" uniqKey="Ooi C">CG Ooi</name></author><author><name sortKey="Yee, Wk" uniqKey="Yee W">WK Yee</name></author><author><name sortKey="Wang, T" uniqKey="Wang T">T Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Poutanen, Sm" uniqKey="Poutanen S">SM Poutanen</name></author><author><name sortKey="Low, D" uniqKey="Low D">D Low</name></author><author><name sortKey="Henry, B" uniqKey="Henry B">B Henry</name></author><author><name sortKey="Finkelstein, S" uniqKey="Finkelstein S">S Finkelstein</name></author><author><name sortKey="Rose, D" uniqKey="Rose D">D Rose</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Lee, N" uniqKey="Lee N">N Lee</name></author><author><name sortKey="Hui, D" uniqKey="Hui D">D Hui</name></author><author><name sortKey="Wu, A" uniqKey="Wu A">A Wu</name></author><author><name sortKey="Chan, P" uniqKey="Chan P">P Chan</name></author><author><name sortKey="Cameron, P" uniqKey="Cameron P">P Cameron</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fowler, Ra" uniqKey="Fowler R">RA Fowler</name></author><author><name sortKey="Lapinsky, Se" uniqKey="Lapinsky S">SE Lapinsky</name></author><author><name sortKey="Hallett, D" uniqKey="Hallett D">D Hallett</name></author><author><name sortKey="Detsky, As" uniqKey="Detsky A">AS Detsky</name></author><author><name sortKey="Sibbald, Wj" uniqKey="Sibbald W">WJ Sibbald</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Peiris, Jsm" uniqKey="Peiris J">JSM Peiris</name></author><author><name sortKey="Yuen, Ky" uniqKey="Yuen K">KY Yuen</name></author><author><name sortKey="Osterhaus, Adme" uniqKey="Osterhaus A">ADME Osterhaus</name></author><author><name sortKey="Stohr, K" uniqKey="Stohr K">K Stohr</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ksiazek, Tg" uniqKey="Ksiazek T">TG Ksiazek</name></author><author><name sortKey="Erdman, D" uniqKey="Erdman D">D Erdman</name></author><author><name sortKey="Goldsmith, Cs" uniqKey="Goldsmith C">CS Goldsmith</name></author><author><name sortKey="Zaki, Sr" uniqKey="Zaki S">SR Zaki</name></author><author><name sortKey="Peret, T" uniqKey="Peret T">T Peret</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Drosten, C" uniqKey="Drosten C">C Drosten</name></author><author><name sortKey="Gunther, S" uniqKey="Gunther S">S Gunther</name></author><author><name sortKey="Preiser, W" uniqKey="Preiser W">W Preiser</name></author><author><name sortKey="Van Der, Ws" uniqKey="Van Der W">WS van der</name></author><author><name sortKey="Brodt, Hr" uniqKey="Brodt H">HR Brodt</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Li, W" uniqKey="Li W">W Li</name></author><author><name sortKey="Shi, Z" uniqKey="Shi Z">Z Shi</name></author><author><name sortKey="Yu, M" uniqKey="Yu M">M Yu</name></author><author><name sortKey="Ren, W" uniqKey="Ren W">W Ren</name></author><author><name sortKey="Smith, C" uniqKey="Smith C">C Smith</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Kusters, Ic" uniqKey="Kusters I">IC Kusters</name></author><author><name sortKey="Matthews, J" uniqKey="Matthews J">J Matthews</name></author><author><name sortKey="Saluzzo, Jf" uniqKey="Saluzzo J">JF Saluzzo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Haagmans, Bl" uniqKey="Haagmans B">BL Haagmans</name></author><author><name sortKey="Boudet, F" uniqKey="Boudet F">F Boudet</name></author><author><name sortKey="Kuiken, T" uniqKey="Kuiken T">T Kuiken</name></author><author><name sortKey="Delang, A" uniqKey="Delang A">A deLang</name></author><author><name sortKey="Martina, Be" uniqKey="Martina B">BE Martina</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="See, Rh" uniqKey="See R">RH See</name></author><author><name sortKey="Zakhartchouk, An" uniqKey="Zakhartchouk A">AN Zakhartchouk</name></author><author><name sortKey="Petric, M" uniqKey="Petric M">M Petric</name></author><author><name sortKey="Lawrence, Dj" uniqKey="Lawrence D">DJ Lawrence</name></author><author><name sortKey="Mok, Cp" uniqKey="Mok C">CP Mok</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Spruth, M" uniqKey="Spruth M">M Spruth</name></author><author><name sortKey="Kistner, O" uniqKey="Kistner O">O Kistner</name></author><author><name sortKey="Savidis Dacho, H" uniqKey="Savidis Dacho H">H Savidis-Dacho</name></author><author><name sortKey="Hitter, E" uniqKey="Hitter E">E Hitter</name></author><author><name sortKey="Crowe, B" uniqKey="Crowe B">B Crowe</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Zhou, Z" uniqKey="Zhou Z">Z Zhou</name></author><author><name sortKey="Post, P" uniqKey="Post P">P Post</name></author><author><name sortKey="Chubet, R" uniqKey="Chubet R">R Chubet</name></author><author><name sortKey="Holtz, K" uniqKey="Holtz K">K Holtz</name></author><author><name sortKey="Mcpherson, C" uniqKey="Mcpherson C">C McPherson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Deming, D" uniqKey="Deming D">D Deming</name></author><author><name sortKey="Sheahan, T" uniqKey="Sheahan T">T Sheahan</name></author><author><name sortKey="Heise, M" uniqKey="Heise M">M Heise</name></author><author><name sortKey="Yount, B" uniqKey="Yount B">B Yount</name></author><author><name sortKey="Davis, N" uniqKey="Davis N">N Davis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Enjuanes, L" uniqKey="Enjuanes L">L Enjuanes</name></author><author><name sortKey="Dediego, Ml" uniqKey="Dediego M">ML DeDiego</name></author><author><name sortKey="Alvarez, E" uniqKey="Alvarez E">E Alvarez</name></author><author><name sortKey="Deming, D" uniqKey="Deming D">D Deming</name></author><author><name sortKey="Sheahan, T" uniqKey="Sheahan T">T Sheahan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lokugamage, Kg" uniqKey="Lokugamage K">KG Lokugamage</name></author><author><name sortKey="Yoshikawa Iwata, N" uniqKey="Yoshikawa Iwata N">N Yoshikawa-Iwata</name></author><author><name sortKey="Ito, N" uniqKey="Ito N">N Ito</name></author><author><name sortKey="Watts, Dm" uniqKey="Watts D">DM Watts</name></author><author><name sortKey="Wyde, Pr" uniqKey="Wyde P">PR Wyde</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yasui, F" uniqKey="Yasui F">F Yasui</name></author><author><name sortKey="Kai, C" uniqKey="Kai C">C Kai</name></author><author><name sortKey="Kitabatake, M" uniqKey="Kitabatake M">M Kitabatake</name></author><author><name sortKey="Inoue, S" uniqKey="Inoue S">S Inoue</name></author><author><name sortKey="Yoneda, M" uniqKey="Yoneda M">M Yoneda</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="See, Rh" uniqKey="See R">RH See</name></author><author><name sortKey="Petric, M" uniqKey="Petric M">M Petric</name></author><author><name sortKey="Lawrence, Dj" uniqKey="Lawrence D">DJ Lawrence</name></author><author><name sortKey="Mok, Cpy" uniqKey="Mok C">CPY Mok</name></author><author><name sortKey="Rowe, T" uniqKey="Rowe T">T Rowe</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lamirande, Ew" uniqKey="Lamirande E">EW Lamirande</name></author><author><name sortKey="Dediego, Ml" uniqKey="Dediego M">ML DeDiego</name></author><author><name sortKey="Roberts, A" uniqKey="Roberts A">A Roberts</name></author><author><name sortKey="Jackson, Jp" uniqKey="Jackson J">JP Jackson</name></author><author><name sortKey="Alvarez, E" uniqKey="Alvarez E">E Alvarez</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lu, B" uniqKey="Lu B">B Lu</name></author><author><name sortKey="Huang, Y" uniqKey="Huang Y">Y Huang</name></author><author><name sortKey="Huang, L" uniqKey="Huang L">L Huang</name></author><author><name sortKey="Li, B" uniqKey="Li B">B Li</name></author><author><name sortKey="Zheng, Z" uniqKey="Zheng Z">Z Zheng</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Roberts, A" uniqKey="Roberts A">A Roberts</name></author><author><name sortKey="Lamirande, Ew" uniqKey="Lamirande E">EW Lamirande</name></author><author><name sortKey="Vogel, L" uniqKey="Vogel L">L Vogel</name></author><author><name sortKey="Baras, B" uniqKey="Baras B">B Baras</name></author><author><name sortKey="Goossens, G" uniqKey="Goossens G">G Goossens</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Du, L" uniqKey="Du L">L Du</name></author><author><name sortKey="Zhao, G" uniqKey="Zhao G">G Zhao</name></author><author><name sortKey="Chan, Ccs" uniqKey="Chan C">CCS Chan</name></author><author><name sortKey="Li, L" uniqKey="Li L">L Li</name></author><author><name sortKey="He, Y" uniqKey="He Y">Y He</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Liu, Yv" uniqKey="Liu Y">YV Liu</name></author><author><name sortKey="Massare, Mj" uniqKey="Massare M">MJ Massare</name></author><author><name sortKey="Barnard, Dl" uniqKey="Barnard D">DL Barnard</name></author><author><name sortKey="Kort, T" uniqKey="Kort T">T Kort</name></author><author><name sortKey="Nathan, M" uniqKey="Nathan M">M Nathan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bolles, M" uniqKey="Bolles M">M Bolles</name></author><author><name sortKey="Deming, D" uniqKey="Deming D">D Deming</name></author><author><name sortKey="Long, K" uniqKey="Long K">K Long</name></author><author><name sortKey="Agnihothram, S" uniqKey="Agnihothram S">S Agnihothram</name></author><author><name sortKey="Whitmore" uniqKey="Whitmore">Whitmore</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lin, J T" uniqKey="Lin J">J-T Lin</name></author><author><name sortKey="Zhang, J S" uniqKey="Zhang J">J-S Zhang</name></author><author><name sortKey="Su, N" uniqKey="Su N">N Su</name></author><author><name sortKey="Xu, J G" uniqKey="Xu J">J-G Xu</name></author><author><name sortKey="Wang, N" uniqKey="Wang N">N Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Martin, Je" uniqKey="Martin J">JE Martin</name></author><author><name sortKey="Louder, Mk" uniqKey="Louder M">MK Louder</name></author><author><name sortKey="Holman, La" uniqKey="Holman L">LA Holman</name></author><author><name sortKey="Gordon, Ij" uniqKey="Gordon I">IJ Gordon</name></author><author><name sortKey="Enama, Me" uniqKey="Enama M">ME Enama</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Perlman, S" uniqKey="Perlman S">S Perlman</name></author><author><name sortKey="Dandekar, Aa" uniqKey="Dandekar A">AA Dandekar</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Castilow, Em" uniqKey="Castilow E">EM Castilow</name></author><author><name sortKey="Olson, Mr" uniqKey="Olson M">MR Olson</name></author><author><name sortKey="Varga, Sm" uniqKey="Varga S">SM Varga</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Collins, Pl" uniqKey="Collins P">PL Collins</name></author><author><name sortKey="Graham, Bs" uniqKey="Graham B">BS Graham</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tseng, Ct" uniqKey="Tseng C">CT Tseng</name></author><author><name sortKey="Huang, C" uniqKey="Huang C">C Huang</name></author><author><name sortKey="Newman, P" uniqKey="Newman P">P Newman</name></author><author><name sortKey="Wang, N" uniqKey="Wang N">N Wang</name></author><author><name sortKey="Narayanan, K" uniqKey="Narayanan K">K Narayanan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Yoshikawa, N" uniqKey="Yoshikawa N">N Yoshikawa</name></author><author><name sortKey="Yoshikawa, T" uniqKey="Yoshikawa T">T Yoshikawa</name></author><author><name sortKey="Hill, T" uniqKey="Hill T">T Hill</name></author><author><name sortKey="Huang, C" uniqKey="Huang C">C Huang</name></author><author><name sortKey="Watts, Dm" uniqKey="Watts D">DM Watts</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Protheroe, C" uniqKey="Protheroe C">C Protheroe</name></author><author><name sortKey="Woodruff, Sa" uniqKey="Woodruff S">SA Woodruff</name></author><author><name sortKey="Depetris, G" uniqKey="Depetris G">G DePetris</name></author><author><name sortKey="Mukkada, V" uniqKey="Mukkada V">V Mukkada</name></author><author><name sortKey="Ochkur, Si" uniqKey="Ochkur S">SI Ochkur</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hsieh, C S" uniqKey="Hsieh C">C-S Hsieh</name></author><author><name sortKey="Macatonia, Se" uniqKey="Macatonia S">SE Macatonia</name></author><author><name sortKey="O Garra, A" uniqKey="O Garra A">A O'Garra</name></author><author><name sortKey="Murphy, Km" uniqKey="Murphy K">KM Murphy</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gorham, Jd" uniqKey="Gorham J">JD Gorham</name></author><author><name sortKey="Guler, Ml" uniqKey="Guler M">ML Guler</name></author><author><name sortKey="Steen, Rg" uniqKey="Steen R">RG Steen</name></author><author><name sortKey="Mackey, Aj" uniqKey="Mackey A">AJ Mackey</name></author><author><name sortKey="Daly, Mj" uniqKey="Daly M">MJ Daly</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Launois, P" uniqKey="Launois P">P Launois</name></author><author><name sortKey="Maillard, I" uniqKey="Maillard I">I Maillard</name></author><author><name sortKey="Pingel, S" uniqKey="Pingel S">S Pingel</name></author><author><name sortKey="Gwihart, Kg" uniqKey="Gwihart K">KG Gwihart</name></author><author><name sortKey="Xenarios, I" uniqKey="Xenarios I">I Xenarios</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kapikian, Az" uniqKey="Kapikian A">AZ Kapikian</name></author><author><name sortKey="Mitchell, Rh" uniqKey="Mitchell R">RH Mitchell</name></author><author><name sortKey="Chanock, Rm" uniqKey="Chanock R">RM Chanock</name></author><author><name sortKey="Shvedoff, Ra" uniqKey="Shvedoff R">RA Shvedoff</name></author><author><name sortKey="Stewart, Ce" uniqKey="Stewart C">CE Stewart</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kim, Hw" uniqKey="Kim H">HW Kim</name></author><author><name sortKey="Canchola, Jg" uniqKey="Canchola J">JG Canchola</name></author><author><name sortKey="Brandt, Cd" uniqKey="Brandt C">CD Brandt</name></author><author><name sortKey="Pyles, G" uniqKey="Pyles G">G Pyles</name></author><author><name sortKey="Chanock, Rm" uniqKey="Chanock R">RM Chanock</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Waris, Me" uniqKey="Waris M">ME Waris</name></author><author><name sortKey="Tsou, C" uniqKey="Tsou C">C Tsou</name></author><author><name sortKey="Erdman, Dd" uniqKey="Erdman D">DD Erdman</name></author><author><name sortKey="Zaki, Sr" uniqKey="Zaki S">SR Zaki</name></author><author><name sortKey="Anderson, Lj" uniqKey="Anderson L">LJ Anderson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Polack, Fp" uniqKey="Polack F">FP Polack</name></author><author><name sortKey="Teng, Mn" uniqKey="Teng M">MN Teng</name></author><author><name sortKey="Collins, Pl" uniqKey="Collins P">PL Collins</name></author><author><name sortKey="Prince, Ga" uniqKey="Prince G">GA Prince</name></author><author><name sortKey="Exner, M" uniqKey="Exner M">M Exner</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Power, Uf" uniqKey="Power U">UF Power</name></author><author><name sortKey="Huss, T" uniqKey="Huss T">T Huss</name></author><author><name sortKey="Michaud, V" uniqKey="Michaud V">V Michaud</name></author><author><name sortKey="Plotnicky Gilquin, H" uniqKey="Plotnicky Gilquin H">H Plotnicky-Gilquin</name></author><author><name sortKey="Bonnefoy, J Y" uniqKey="Bonnefoy J">J-Y Bonnefoy</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Weiss, Rc" uniqKey="Weiss R">RC Weiss</name></author><author><name sortKey="Scott, Fw" uniqKey="Scott F">FW Scott</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wentworth, De" uniqKey="Wentworth D">DE Wentworth</name></author><author><name sortKey="Gillim Ross, L" uniqKey="Gillim Ross L">L Gillim-Ross</name></author><author><name sortKey="Espina, N" uniqKey="Espina N">N Espina</name></author><author><name sortKey="Bernard, Ka" uniqKey="Bernard K">KA Bernard</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Subbarao, K" uniqKey="Subbarao K">K Subbarao</name></author><author><name sortKey="Mcauliffe, J" uniqKey="Mcauliffe J">J McAuliffe</name></author><author><name sortKey="Vogel, L" uniqKey="Vogel L">L Vogel</name></author><author><name sortKey="Fahle, G" uniqKey="Fahle G">G Fahle</name></author><author><name sortKey="Fischer, S" uniqKey="Fischer S">S Fischer</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jordan, Mb" uniqKey="Jordan M">MB Jordan</name></author><author><name sortKey="Mills, Dm" uniqKey="Mills D">DM Mills</name></author><author><name sortKey="Kappler, J" uniqKey="Kappler J">J Kappler</name></author><author><name sortKey="Marrack, P" uniqKey="Marrack P">P Marrack</name></author><author><name sortKey="Cambier, Jc" uniqKey="Cambier J">JC Cambier</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Garcon, N" uniqKey="Garcon N">N Garcon</name></author><author><name sortKey="Chomez, P" uniqKey="Chomez P">P Chomez</name></author><author><name sortKey="Van Mechelen, M" uniqKey="Van Mechelen M">M Van Mechelen</name></author></analytic></biblStruct></listBibl></div1></back></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Ncbi/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002490 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd -nk 002490 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Ncbi
|étape= Curation
|type= RBID
|clé= PMC:3335060
|texte= Immunization with SARS Coronavirus Vaccines Leads to Pulmonary Immunopathology on Challenge with the SARS Virus
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/RBID.i -Sk "pubmed:22536382" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |