CD8+ T cell response in HLA-A*0201 transgenic mice is elicited by epitopes from SARS-CoV S protein.
Identifieur interne : 002191 ( Ncbi/Curation ); précédent : 002190; suivant : 002192CD8+ T cell response in HLA-A*0201 transgenic mice is elicited by epitopes from SARS-CoV S protein.
Auteurs : Kai Zhao [République populaire de Chine] ; Binyan Yang ; Yanquan Xu ; Changyou WuSource :
- Vaccine [ 1873-2518 ] ; 2010.
Descripteurs français
- KwdFr :
- ADN viral (immunologie), Animaux, Antigène HLA-A2, Antigènes HLA-A, Déterminants antigéniques des lymphocytes T (immunologie), Facteur de nécrose tumorale alpha (immunologie), Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (immunologie), Immunité cellulaire, Interféron gamma (immunologie), Interleukine-2 (immunologie), Lymphocytes T CD8+ (immunologie), Mémoire immunologique, Protéines de l'enveloppe virale (immunologie), Souris, Souris transgéniques, Syndrome respiratoire aigu sévère (immunologie), Vaccins antiviraux (immunologie), Vaccins à ADN (immunologie), Virus du SRAS (immunologie).
- MESH :
- immunologie : ADN viral, Déterminants antigéniques des lymphocytes T, Facteur de nécrose tumorale alpha, Glycoprotéines membranaires, Interféron gamma, Interleukine-2, Lymphocytes T CD8+, Protéines de l'enveloppe virale, Syndrome respiratoire aigu sévère, Vaccins antiviraux, Vaccins à ADN, Virus du SRAS.
- Animaux, Antigène HLA-A2, Antigènes HLA-A, Glycoprotéine de spicule des coronavirus, Immunité cellulaire, Mémoire immunologique, Souris, Souris transgéniques.
English descriptors
- KwdEn :
- Animals, CD8-Positive T-Lymphocytes (immunology), DNA, Viral (immunology), Epitopes, T-Lymphocyte (immunology), HLA-A Antigens, HLA-A2 Antigen, Immunity, Cellular, Immunologic Memory, Interferon-gamma (immunology), Interleukin-2 (immunology), Membrane Glycoproteins (immunology), Mice, Mice, Transgenic, SARS Virus (immunology), Severe Acute Respiratory Syndrome (immunology), Spike Glycoprotein, Coronavirus, Tumor Necrosis Factor-alpha (immunology), Vaccines, DNA (immunology), Viral Envelope Proteins (immunology), Viral Vaccines (immunology).
- MESH :
- chemical , immunology : DNA, Viral, Epitopes, T-Lymphocyte, Interferon-gamma, Interleukin-2, Membrane Glycoproteins, Tumor Necrosis Factor-alpha, Vaccines, DNA, Viral Envelope Proteins, Viral Vaccines.
- immunology : CD8-Positive T-Lymphocytes, SARS Virus, Severe Acute Respiratory Syndrome.
- Animals, HLA-A Antigens, HLA-A2 Antigen, Immunity, Cellular, Immunologic Memory, Mice, Mice, Transgenic, Spike Glycoprotein, Coronavirus.
Abstract
Cytotoxic CD8(+) T lymphocytes (CTLs) play an important role in antiviral immunity. Several human HLA-A*0201 restricted CTL epitopes of severe acute respiratory syndrome (SARS) spike (S) protein have been identified in HLA-A*0201 transgenic (Tg) mice, but the mechanisms and properties of immune responses are still not well understood. In this study, HLA-A*0201 Tg mice were primed intramuscularly with SARS S DNA and boosted subcutaneously with HLA-A*0201 restricted peptides. The lymphocytes from draining lymph nodes, spleens and lungs were stimulated with the cognate peptides. Three different methods (ELISA, ELISPOT and FACS) were used to evaluate the immune responses during short and long periods of time after immunization. Results showed that peptide-specific CD8(+) T cells secreted IFN-γ, TNF-α and IL-2 and expressed CD107a/b on cell surface. IFN-γ(+)CD8(+) T cells and CD107a/b(+)CD8(+) T cells distributed throughout the lymphoid and non-lymphoid tissues, but the frequency of peptide-specific CD8(+) T cells was higher in lungs than in spleens and lymph nodes. The phenotype of the CD8(+) T cells was characterized based on the expression of IFN-γ. Most of the HLA-A*0201 restricted peptide-specific CD8(+) T cells represented a memory subset with CD45RB(high) and CD62L(low). Taken together, these data demonstrate that immunization with SARS S DNA and HLA-A*0201 restricted peptides can elicit antigen-specific CD8(+) T cell immune responses which may have a significant implication in the long-term protection. We provide novel information in cellular immune responses of SARS S antigen-specific CD8(+) T cells, which are important in the development of vaccine against SARS-CoV infection.
DOI: 10.1016/j.vaccine.2010.08.013
PubMed: 20709007
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pubmed:20709007Le document en format XML
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sortKey="Xu, Yanquan" sort="Xu, Yanquan" uniqKey="Xu Y" first="Yanquan" last="Xu">Yanquan Xu</name></author><author><name sortKey="Wu, Changyou" sort="Wu, Changyou" uniqKey="Wu C" first="Changyou" last="Wu">Changyou Wu</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2010">2010</date><idno type="RBID">pubmed:20709007</idno><idno type="pmid">20709007</idno><idno type="doi">10.1016/j.vaccine.2010.08.013</idno><idno type="wicri:Area/PubMed/Corpus">001648</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001648</idno><idno type="wicri:Area/PubMed/Curation">001648</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001648</idno><idno type="wicri:Area/PubMed/Checkpoint">001704</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001704</idno><idno type="wicri:Area/Ncbi/Merge">002191</idno><idno 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last="Yang">Binyan Yang</name></author><author><name sortKey="Xu, Yanquan" sort="Xu, Yanquan" uniqKey="Xu Y" first="Yanquan" last="Xu">Yanquan Xu</name></author><author><name sortKey="Wu, Changyou" sort="Wu, Changyou" uniqKey="Wu C" first="Changyou" last="Wu">Changyou Wu</name></author></analytic><series><title level="j">Vaccine</title><idno type="eISSN">1873-2518</idno><imprint><date when="2010" type="published">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>CD8-Positive T-Lymphocytes (immunology)</term><term>DNA, Viral (immunology)</term><term>Epitopes, T-Lymphocyte (immunology)</term><term>HLA-A Antigens</term><term>HLA-A2 Antigen</term><term>Immunity, Cellular</term><term>Immunologic Memory</term><term>Interferon-gamma (immunology)</term><term>Interleukin-2 (immunology)</term><term>Membrane Glycoproteins (immunology)</term><term>Mice</term><term>Mice, Transgenic</term><term>SARS Virus (immunology)</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>Spike Glycoprotein, Coronavirus</term><term>Tumor Necrosis Factor-alpha (immunology)</term><term>Vaccines, DNA (immunology)</term><term>Viral Envelope Proteins (immunology)</term><term>Viral Vaccines (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN viral (immunologie)</term><term>Animaux</term><term>Antigène HLA-A2</term><term>Antigènes HLA-A</term><term>Déterminants antigéniques des lymphocytes T (immunologie)</term><term>Facteur de nécrose tumorale alpha (immunologie)</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (immunologie)</term><term>Immunité cellulaire</term><term>Interféron gamma (immunologie)</term><term>Interleukine-2 (immunologie)</term><term>Lymphocytes T CD8+ (immunologie)</term><term>Mémoire immunologique</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Souris</term><term>Souris transgéniques</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Vaccins antiviraux (immunologie)</term><term>Vaccins à ADN (immunologie)</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>DNA, Viral</term><term>Epitopes, T-Lymphocyte</term><term>Interferon-gamma</term><term>Interleukin-2</term><term>Membrane Glycoproteins</term><term>Tumor Necrosis Factor-alpha</term><term>Vaccines, DNA</term><term>Viral Envelope Proteins</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>ADN viral</term><term>Déterminants antigéniques des lymphocytes T</term><term>Facteur de nécrose tumorale alpha</term><term>Glycoprotéines membranaires</term><term>Interféron gamma</term><term>Interleukine-2</term><term>Lymphocytes T CD8+</term><term>Protéines de l'enveloppe virale</term><term>Syndrome respiratoire aigu sévère</term><term>Vaccins antiviraux</term><term>Vaccins à ADN</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>CD8-Positive T-Lymphocytes</term><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>HLA-A Antigens</term><term>HLA-A2 Antigen</term><term>Immunity, Cellular</term><term>Immunologic Memory</term><term>Mice</term><term>Mice, Transgenic</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Antigène HLA-A2</term><term>Antigènes HLA-A</term><term>Glycoprotéine de spicule des coronavirus</term><term>Immunité cellulaire</term><term>Mémoire immunologique</term><term>Souris</term><term>Souris transgéniques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Cytotoxic CD8(+) T lymphocytes (CTLs) play an important role in antiviral immunity. Several human HLA-A*0201 restricted CTL epitopes of severe acute respiratory syndrome (SARS) spike (S) protein have been identified in HLA-A*0201 transgenic (Tg) mice, but the mechanisms and properties of immune responses are still not well understood. In this study, HLA-A*0201 Tg mice were primed intramuscularly with SARS S DNA and boosted subcutaneously with HLA-A*0201 restricted peptides. The lymphocytes from draining lymph nodes, spleens and lungs were stimulated with the cognate peptides. Three different methods (ELISA, ELISPOT and FACS) were used to evaluate the immune responses during short and long periods of time after immunization. Results showed that peptide-specific CD8(+) T cells secreted IFN-γ, TNF-α and IL-2 and expressed CD107a/b on cell surface. IFN-γ(+)CD8(+) T cells and CD107a/b(+)CD8(+) T cells distributed throughout the lymphoid and non-lymphoid tissues, but the frequency of peptide-specific CD8(+) T cells was higher in lungs than in spleens and lymph nodes. The phenotype of the CD8(+) T cells was characterized based on the expression of IFN-γ. Most of the HLA-A*0201 restricted peptide-specific CD8(+) T cells represented a memory subset with CD45RB(high) and CD62L(low). Taken together, these data demonstrate that immunization with SARS S DNA and HLA-A*0201 restricted peptides can elicit antigen-specific CD8(+) T cell immune responses which may have a significant implication in the long-term protection. We provide novel information in cellular immune responses of SARS S antigen-specific CD8(+) T cells, which are important in the development of vaccine against SARS-CoV infection.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
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