SARS spike protein induces phenotypic conversion of human B cells to macrophage-like cells.
Identifieur interne : 002178 ( Ncbi/Curation ); précédent : 002177; suivant : 002179SARS spike protein induces phenotypic conversion of human B cells to macrophage-like cells.
Auteurs : Shu-Fen Chiang [Taïwan] ; Tze-Yi Lin ; Kuan-Chih Chow ; Shiow-Her ChiouSource :
- Molecular immunology [ 1872-9142 ] ; 2010.
Descripteurs français
- KwdFr :
- Analyse de profil d'expression de gènes, Animaux, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (immunologie), Humains, Lignée cellulaire, Lymphocytes B (immunologie), Lymphome B (immunologie), Macrophages (immunologie), Mâle, Phénotype, Protéines de l'enveloppe virale (immunologie), Protéines recombinantes (immunologie), Souris, Souris de lignée BALB C, Virus du SRAS (immunologie).
- MESH :
- immunologie : Glycoprotéines membranaires, Lymphocytes B, Lymphome B, Macrophages, Protéines de l'enveloppe virale, Protéines recombinantes, Virus du SRAS.
- Analyse de profil d'expression de gènes, Animaux, Glycoprotéine de spicule des coronavirus, Humains, Lignée cellulaire, Mâle, Phénotype, Souris, Souris de lignée BALB C.
English descriptors
- KwdEn :
- Animals, B-Lymphocytes (immunology), Cell Line, Gene Expression Profiling, Humans, Lymphoma, B-Cell (immunology), Macrophages (immunology), Male, Membrane Glycoproteins (immunology), Mice, Mice, Inbred BALB C, Phenotype, Recombinant Proteins (immunology), SARS Virus (immunology), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (immunology).
- MESH :
- chemical , immunology : Membrane Glycoproteins, Recombinant Proteins, Viral Envelope Proteins.
- immunology : B-Lymphocytes, Lymphoma, B-Cell, Macrophages, SARS Virus.
- Animals, Cell Line, Gene Expression Profiling, Humans, Male, Mice, Mice, Inbred BALB C, Phenotype, Spike Glycoprotein, Coronavirus.
Abstract
Massive aggregations of macrophages are frequently detected in afflicted lungs of patients with severe acute respiratory syndrome-associated coronavirus (SARS-CoV) infection. In vitro, ectopic expression of transcription factors, in particular CCAAT/enhancer-binding protein alpha (C/EBPα) and C/EBPβ, can convert B cells into functional macrophages. However, little is known about the specific ligands responsible for such phenotype conversion. Here, we investigated whether spike protein of SARS-CoV can act as a ligand to trigger the conversion of B cells to macrophages. We transduced SARS-CoV spike protein-displayed recombinant baculovirus (SSDRB), vAtEpGS688, into peripheral B cells and B lymphoma cells. Cell surface expression of CD19 or Mac-1 (CD11b) was determined by flow cytometry. SSDRB-mediated changes in gene expression profiles of B lymphoma cells were analyzed by microarray. In this report, we showed that spike protein of SARS virus could induce phenotypic conversion of human B cells, either from peripheral blood or B lymphoma cells, to macrophage-like cells that were steadily losing the B-cell marker CD19 and in turn expressing the macrophage-specific marker Mac-1. Furthermore, we found that SSDRB enhanced the expression of CD86, hypoxia-inducible factor-1α (HIF1α), suppressor of cytokine signaling (SOCS or STAT-induced STAT inhibitor)-3, C/EBPβ, insulin-like growth factor-binding protein 3 (IGFBP3), Krüpple-like factor (KLF)-5, and CD54, without marked influence on C/EBPα or PU.1 expression in transduced cells. Prolonged exposure to hypoxia could also induce macrophage-like conversion of B cells. These macrophage-like cells were defective in phagocytosis of red fluorescent beads. In conclusion, our results suggest that conversion of B cells to macrophage-like cells, similar to a pathophysiological response, could be mediated by a devastating viral ligand, in particular spike protein of SARS virus, or in combination with severe local hypoxia, which is a condition often observed in afflicted lungs of SARS patients.
DOI: 10.1016/j.molimm.2010.06.014
PubMed: 20667598
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :001660
- to stream PubMed, to step Curation: Pour aller vers cette notice dans l'étape Curation :001660
- to stream PubMed, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :001600
- to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :002178
Links to Exploration step
pubmed:20667598Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">SARS spike protein induces phenotypic conversion of human B cells to macrophage-like cells.</title>
<author><name sortKey="Chiang, Shu Fen" sort="Chiang, Shu Fen" uniqKey="Chiang S" first="Shu-Fen" last="Chiang">Shu-Fen Chiang</name>
<affiliation wicri:level="1"><nlm:affiliation>Graduate Institute of Microbiology and Public Health, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 40227, Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Graduate Institute of Microbiology and Public Health, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 40227</wicri:regionArea>
<wicri:noRegion>Taichung 40227</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lin, Tze Yi" sort="Lin, Tze Yi" uniqKey="Lin T" first="Tze-Yi" last="Lin">Tze-Yi Lin</name>
</author>
<author><name sortKey="Chow, Kuan Chih" sort="Chow, Kuan Chih" uniqKey="Chow K" first="Kuan-Chih" last="Chow">Kuan-Chih Chow</name>
</author>
<author><name sortKey="Chiou, Shiow Her" sort="Chiou, Shiow Her" uniqKey="Chiou S" first="Shiow-Her" last="Chiou">Shiow-Her Chiou</name>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PubMed</idno>
<date when="2010">2010</date>
<idno type="RBID">pubmed:20667598</idno>
<idno type="pmid">20667598</idno>
<idno type="doi">10.1016/j.molimm.2010.06.014</idno>
<idno type="wicri:Area/PubMed/Corpus">001660</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001660</idno>
<idno type="wicri:Area/PubMed/Curation">001660</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001660</idno>
<idno type="wicri:Area/PubMed/Checkpoint">001600</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001600</idno>
<idno type="wicri:Area/Ncbi/Merge">002178</idno>
<idno type="wicri:Area/Ncbi/Curation">002178</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en">SARS spike protein induces phenotypic conversion of human B cells to macrophage-like cells.</title>
<author><name sortKey="Chiang, Shu Fen" sort="Chiang, Shu Fen" uniqKey="Chiang S" first="Shu-Fen" last="Chiang">Shu-Fen Chiang</name>
<affiliation wicri:level="1"><nlm:affiliation>Graduate Institute of Microbiology and Public Health, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 40227, Taiwan.</nlm:affiliation>
<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Graduate Institute of Microbiology and Public Health, National Chung Hsing University, 250 Kuo Kuang Road, Taichung 40227</wicri:regionArea>
<wicri:noRegion>Taichung 40227</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Lin, Tze Yi" sort="Lin, Tze Yi" uniqKey="Lin T" first="Tze-Yi" last="Lin">Tze-Yi Lin</name>
</author>
<author><name sortKey="Chow, Kuan Chih" sort="Chow, Kuan Chih" uniqKey="Chow K" first="Kuan-Chih" last="Chow">Kuan-Chih Chow</name>
</author>
<author><name sortKey="Chiou, Shiow Her" sort="Chiou, Shiow Her" uniqKey="Chiou S" first="Shiow-Her" last="Chiou">Shiow-Her Chiou</name>
</author>
</analytic>
<series><title level="j">Molecular immunology</title>
<idno type="eISSN">1872-9142</idno>
<imprint><date when="2010" type="published">2010</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>B-Lymphocytes (immunology)</term>
<term>Cell Line</term>
<term>Gene Expression Profiling</term>
<term>Humans</term>
<term>Lymphoma, B-Cell (immunology)</term>
<term>Macrophages (immunology)</term>
<term>Male</term>
<term>Membrane Glycoproteins (immunology)</term>
<term>Mice</term>
<term>Mice, Inbred BALB C</term>
<term>Phenotype</term>
<term>Recombinant Proteins (immunology)</term>
<term>SARS Virus (immunology)</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Viral Envelope Proteins (immunology)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de profil d'expression de gènes</term>
<term>Animaux</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires (immunologie)</term>
<term>Humains</term>
<term>Lignée cellulaire</term>
<term>Lymphocytes B (immunologie)</term>
<term>Lymphome B (immunologie)</term>
<term>Macrophages (immunologie)</term>
<term>Mâle</term>
<term>Phénotype</term>
<term>Protéines de l'enveloppe virale (immunologie)</term>
<term>Protéines recombinantes (immunologie)</term>
<term>Souris</term>
<term>Souris de lignée BALB C</term>
<term>Virus du SRAS (immunologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Membrane Glycoproteins</term>
<term>Recombinant Proteins</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Glycoprotéines membranaires</term>
<term>Lymphocytes B</term>
<term>Lymphome B</term>
<term>Macrophages</term>
<term>Protéines de l'enveloppe virale</term>
<term>Protéines recombinantes</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>B-Lymphocytes</term>
<term>Lymphoma, B-Cell</term>
<term>Macrophages</term>
<term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Cell Line</term>
<term>Gene Expression Profiling</term>
<term>Humans</term>
<term>Male</term>
<term>Mice</term>
<term>Mice, Inbred BALB C</term>
<term>Phenotype</term>
<term>Spike Glycoprotein, Coronavirus</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Analyse de profil d'expression de gènes</term>
<term>Animaux</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Humains</term>
<term>Lignée cellulaire</term>
<term>Mâle</term>
<term>Phénotype</term>
<term>Souris</term>
<term>Souris de lignée BALB C</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en">Massive aggregations of macrophages are frequently detected in afflicted lungs of patients with severe acute respiratory syndrome-associated coronavirus (SARS-CoV) infection. In vitro, ectopic expression of transcription factors, in particular CCAAT/enhancer-binding protein alpha (C/EBPα) and C/EBPβ, can convert B cells into functional macrophages. However, little is known about the specific ligands responsible for such phenotype conversion. Here, we investigated whether spike protein of SARS-CoV can act as a ligand to trigger the conversion of B cells to macrophages. We transduced SARS-CoV spike protein-displayed recombinant baculovirus (SSDRB), vAtEpGS688, into peripheral B cells and B lymphoma cells. Cell surface expression of CD19 or Mac-1 (CD11b) was determined by flow cytometry. SSDRB-mediated changes in gene expression profiles of B lymphoma cells were analyzed by microarray. In this report, we showed that spike protein of SARS virus could induce phenotypic conversion of human B cells, either from peripheral blood or B lymphoma cells, to macrophage-like cells that were steadily losing the B-cell marker CD19 and in turn expressing the macrophage-specific marker Mac-1. Furthermore, we found that SSDRB enhanced the expression of CD86, hypoxia-inducible factor-1α (HIF1α), suppressor of cytokine signaling (SOCS or STAT-induced STAT inhibitor)-3, C/EBPβ, insulin-like growth factor-binding protein 3 (IGFBP3), Krüpple-like factor (KLF)-5, and CD54, without marked influence on C/EBPα or PU.1 expression in transduced cells. Prolonged exposure to hypoxia could also induce macrophage-like conversion of B cells. These macrophage-like cells were defective in phagocytosis of red fluorescent beads. In conclusion, our results suggest that conversion of B cells to macrophage-like cells, similar to a pathophysiological response, could be mediated by a devastating viral ligand, in particular spike protein of SARS virus, or in combination with severe local hypoxia, which is a condition often observed in afflicted lungs of SARS patients.</div>
</front>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Ncbi/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 002178 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd -nk 002178 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= SrasV1 |flux= Ncbi |étape= Curation |type= RBID |clé= pubmed:20667598 |texte= SARS spike protein induces phenotypic conversion of human B cells to macrophage-like cells. }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/RBID.i -Sk "pubmed:20667598" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd \ | NlmPubMed2Wicri -a SrasV1
![]() | This area was generated with Dilib version V0.6.33. | ![]() |