Angiotensin-converting enzyme 2 (ACE2) proteins of different bat species confer variable susceptibility to SARS-CoV entry
Identifieur interne : 002156 ( Ncbi/Curation ); précédent : 002155; suivant : 002157Angiotensin-converting enzyme 2 (ACE2) proteins of different bat species confer variable susceptibility to SARS-CoV entry
Auteurs : Yuxuan Hou ; Cheng Peng ; Meng Yu ; Yan Li ; Zhenggang Han ; Fang Li [États-Unis] ; Lin-Fa Wang ; Zhengli ShiSource :
- Archives of Virology [ 0304-8608 ] ; 2010.
Descripteurs français
- KwdFr :
- ARN viral (génétique), Alignement de séquences, Analyse de séquence d'ADN, Animaux, Cellules HeLa, Chiroptera, Données de séquences moléculaires, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (métabolisme), Humains, Liaison aux protéines, Modèles moléculaires, Mutagenèse dirigée, Peptidyl-Dipeptidase A (génétique), Peptidyl-Dipeptidase A (métabolisme), Peptidyl-Dipeptidase A (physiologie), Protéines de l'enveloppe virale (métabolisme), Protéines mutantes (génétique), Protéines mutantes (métabolisme), Pénétration virale, Récepteurs viraux (génétique), Récepteurs viraux (métabolisme), Récepteurs viraux (physiologie), Réservoirs d'agents pathogènes, Structure tertiaire des protéines, Substitution d'acide aminé, Séquence d'acides aminés, Variation génétique, Virus du SRAS (physiologie).
- MESH :
- génétique : ARN viral, Peptidyl-Dipeptidase A, Protéines mutantes, Récepteurs viraux.
- métabolisme : Glycoprotéines membranaires, Peptidyl-Dipeptidase A, Protéines de l'enveloppe virale, Protéines mutantes, Récepteurs viraux.
- physiologie : Peptidyl-Dipeptidase A, Récepteurs viraux, Virus du SRAS.
- Alignement de séquences, Analyse de séquence d'ADN, Animaux, Cellules HeLa, Chiroptera, Données de séquences moléculaires, Glycoprotéine de spicule des coronavirus, Humains, Liaison aux protéines, Modèles moléculaires, Mutagenèse dirigée, Pénétration virale, Réservoirs d'agents pathogènes, Structure tertiaire des protéines, Substitution d'acide aminé, Séquence d'acides aminés, Variation génétique.
English descriptors
- KwdEn :
- Amino Acid Sequence, Amino Acid Substitution, Animals, Chiroptera, Disease Reservoirs, Genetic Variation, HeLa Cells, Humans, Membrane Glycoproteins (metabolism), Models, Molecular, Molecular Sequence Data, Mutagenesis, Site-Directed, Mutant Proteins (genetics), Mutant Proteins (metabolism), Peptidyl-Dipeptidase A (genetics), Peptidyl-Dipeptidase A (metabolism), Peptidyl-Dipeptidase A (physiology), Protein Binding, Protein Structure, Tertiary, RNA, Viral (genetics), Receptors, Virus (genetics), Receptors, Virus (metabolism), Receptors, Virus (physiology), SARS Virus (physiology), Sequence Alignment, Sequence Analysis, DNA, Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (metabolism), Virus Internalization.
- MESH :
- chemical , genetics : Mutant Proteins, Peptidyl-Dipeptidase A, RNA, Viral, Receptors, Virus.
- chemical , metabolism : Membrane Glycoproteins, Mutant Proteins, Peptidyl-Dipeptidase A, Receptors, Virus, Viral Envelope Proteins.
- chemical , physiology : Peptidyl-Dipeptidase A, Receptors, Virus.
- physiology : SARS Virus.
- Amino Acid Sequence, Amino Acid Substitution, Animals, Chiroptera, Disease Reservoirs, Genetic Variation, HeLa Cells, Humans, Models, Molecular, Molecular Sequence Data, Mutagenesis, Site-Directed, Protein Binding, Protein Structure, Tertiary, Sequence Alignment, Sequence Analysis, DNA, Spike Glycoprotein, Coronavirus, Virus Internalization.
Abstract
The discovery of SARS-like coronavirus in bats suggests that bats could be the natural reservoir of SARS-CoV. However, previous studies indicated the angiotensin-converting enzyme 2 (ACE2) protein, a known SARS-CoV receptor, from a horseshoe bat was unable to act as a functional receptor for SARS-CoV. Here, we extended our previous study to ACE2 molecules from seven additional bat species and tested their interactions with human SARS-CoV spike protein using both HIV-based pseudotype and live SARS-CoV infection assays. The results show that ACE2s of
The online version of this article (doi:10.1007/s00705-010-0729-6) contains supplementary material, which is available to authorized users.
Url:
DOI: 10.1007/s00705-010-0729-6
PubMed: 20567988
PubMed Central: 7086629
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Yuxuan Hou<affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.9227.e</institution-id><institution-id institution-id-type="ISNI">0000000119573309</institution-id><institution>State Key Laboratory of Virology, Wuhan Institute of Virology,</institution><institution>Chinese Academy of Sciences (CAS),</institution></institution-wrap>Wuhan, 430071 Hubei China</nlm:aff><wicri:noCountry code="subfield">430071 Hubei China</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.9227.e</institution-id><institution-id institution-id-type="ISNI">0000000119573309</institution-id><institution>State Key Laboratory of Virology, Wuhan Institute of Virology,</institution><institution>Chinese Academy of Sciences (CAS),</institution></institution-wrap>Wuhan, 430071 Hubei China</nlm:aff><wicri:noCountry code="subfield">430071 Hubei China</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.413322.5</institution-id><institution-id institution-id-type="ISNI">0000 0001 2188 8254</institution-id><institution>Australian Animal Health Laboratory,</institution><institution>Commonwealth Scientific and Industrial Research Organization Livestock Industries,</institution></institution-wrap>PO Bag 24, Geelong, VIC 3220 Australia</nlm:aff><wicri:noCountry code="subfield">VIC 3220 Australia</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.9227.e</institution-id><institution-id institution-id-type="ISNI">0000000119573309</institution-id><institution>State Key Laboratory of Virology, Wuhan Institute of Virology,</institution><institution>Chinese Academy of Sciences (CAS),</institution></institution-wrap>Wuhan, 430071 Hubei China</nlm:aff><wicri:noCountry code="subfield">430071 Hubei China</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.9227.e</institution-id><institution-id institution-id-type="ISNI">0000000119573309</institution-id><institution>State Key Laboratory of Virology, Wuhan Institute of Virology,</institution><institution>Chinese Academy of Sciences (CAS),</institution></institution-wrap>Wuhan, 430071 Hubei China</nlm:aff><wicri:noCountry code="subfield">430071 Hubei China</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.413322.5</institution-id><institution-id institution-id-type="ISNI">0000 0001 2188 8254</institution-id><institution>Australian Animal Health Laboratory,</institution><institution>Commonwealth Scientific and Industrial Research Organization Livestock Industries,</institution></institution-wrap>PO Bag 24, Geelong, VIC 3220 Australia</nlm:aff><wicri:noCountry code="subfield">VIC 3220 Australia</wicri:noCountry></affiliation><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.9227.e</institution-id><institution-id institution-id-type="ISNI">0000000119573309</institution-id><institution>State Key Laboratory of Virology, Wuhan Institute of Virology,</institution><institution>Chinese Academy of Sciences (CAS),</institution></institution-wrap>Wuhan, 430071 Hubei China</nlm:aff><wicri:noCountry code="subfield">430071 Hubei China</wicri:noCountry></affiliation>Le document en format XML
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entry</title><author><name sortKey="Hou, Yuxuan" sort="Hou, Yuxuan" uniqKey="Hou Y" first="Yuxuan" last="Hou">Yuxuan Hou</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.9227.e</institution-id><institution-id institution-id-type="ISNI">0000000119573309</institution-id><institution>State Key Laboratory of Virology, Wuhan Institute of Virology,</institution><institution>Chinese Academy of Sciences (CAS),</institution></institution-wrap>Wuhan, 430071 Hubei China</nlm:aff><wicri:noCountry code="subfield">430071 Hubei China</wicri:noCountry></affiliation></author><author><name sortKey="Peng, Cheng" sort="Peng, Cheng" uniqKey="Peng C" first="Cheng" last="Peng">Cheng Peng</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.9227.e</institution-id><institution-id institution-id-type="ISNI">0000000119573309</institution-id><institution>State Key Laboratory of Virology, Wuhan Institute of 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China</nlm:aff><wicri:noCountry code="subfield">430071 Hubei China</wicri:noCountry></affiliation></author><author><name sortKey="Li, Fang" sort="Li, Fang" uniqKey="Li F" first="Fang" last="Li">Fang Li</name><affiliation wicri:level="2"><nlm:aff id="Aff3"><institution-wrap><institution-id institution-id-type="GRID">grid.17635.36</institution-id><institution-id institution-id-type="ISNI">0000000419368657</institution-id><institution>Department of Pharmacology,</institution><institution>University of Minnesota Medical School,</institution></institution-wrap>Minneapolis, MN 55455 USA</nlm:aff><country xml:lang="fr">États-Unis</country><placeName><region type="state">Minnesota</region></placeName><wicri:cityArea>Minneapolis</wicri:cityArea></affiliation></author><author><name sortKey="Wang, Lin Fa" sort="Wang, Lin Fa" uniqKey="Wang L" first="Lin-Fa" last="Wang">Lin-Fa Wang</name><affiliation><nlm:aff id="Aff2"><institution-wrap><institution-id institution-id-type="GRID">grid.413322.5</institution-id><institution-id institution-id-type="ISNI">0000 0001 2188 8254</institution-id><institution>Australian Animal Health Laboratory,</institution><institution>Commonwealth Scientific and Industrial Research Organization Livestock Industries,</institution></institution-wrap>PO Bag 24, Geelong, VIC 3220 Australia</nlm:aff><wicri:noCountry code="subfield">VIC 3220 Australia</wicri:noCountry></affiliation></author><author><name sortKey="Shi, Zhengli" sort="Shi, Zhengli" uniqKey="Shi Z" first="Zhengli" last="Shi">Zhengli Shi</name><affiliation><nlm:aff id="Aff1"><institution-wrap><institution-id institution-id-type="GRID">grid.9227.e</institution-id><institution-id institution-id-type="ISNI">0000000119573309</institution-id><institution>State Key Laboratory of Virology, Wuhan Institute of Virology,</institution><institution>Chinese Academy of Sciences (CAS),</institution></institution-wrap>Wuhan, 430071 Hubei China</nlm:aff><wicri:noCountry code="subfield">430071 Hubei China</wicri:noCountry></affiliation></author></analytic><series><title level="j">Archives of Virology</title><idno type="ISSN">0304-8608</idno><idno type="eISSN">1432-8798</idno><imprint><date when="2010">2010</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Amino Acid Substitution</term><term>Animals</term><term>Chiroptera</term><term>Disease Reservoirs</term><term>Genetic Variation</term><term>HeLa Cells</term><term>Humans</term><term>Membrane Glycoproteins (metabolism)</term><term>Models, Molecular</term><term>Molecular Sequence Data</term><term>Mutagenesis, Site-Directed</term><term>Mutant Proteins (genetics)</term><term>Mutant Proteins (metabolism)</term><term>Peptidyl-Dipeptidase A (genetics)</term><term>Peptidyl-Dipeptidase A (metabolism)</term><term>Peptidyl-Dipeptidase A (physiology)</term><term>Protein Binding</term><term>Protein Structure, Tertiary</term><term>RNA, Viral (genetics)</term><term>Receptors, Virus (genetics)</term><term>Receptors, Virus (metabolism)</term><term>Receptors, Virus (physiology)</term><term>SARS Virus (physiology)</term><term>Sequence Alignment</term><term>Sequence Analysis, DNA</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Envelope Proteins (metabolism)</term><term>Virus Internalization</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (génétique)</term><term>Alignement de séquences</term><term>Analyse de séquence d'ADN</term><term>Animaux</term><term>Cellules HeLa</term><term>Chiroptera</term><term>Données de séquences moléculaires</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires (métabolisme)</term><term>Humains</term><term>Liaison aux protéines</term><term>Modèles moléculaires</term><term>Mutagenèse dirigée</term><term>Peptidyl-Dipeptidase A (génétique)</term><term>Peptidyl-Dipeptidase A (métabolisme)</term><term>Peptidyl-Dipeptidase A (physiologie)</term><term>Protéines de l'enveloppe virale (métabolisme)</term><term>Protéines mutantes (génétique)</term><term>Protéines mutantes (métabolisme)</term><term>Pénétration virale</term><term>Récepteurs viraux (génétique)</term><term>Récepteurs viraux (métabolisme)</term><term>Récepteurs viraux (physiologie)</term><term>Réservoirs d'agents pathogènes</term><term>Structure tertiaire des protéines</term><term>Substitution d'acide aminé</term><term>Séquence d'acides aminés</term><term>Variation génétique</term><term>Virus du SRAS (physiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Mutant Proteins</term><term>Peptidyl-Dipeptidase A</term><term>RNA, Viral</term><term>Receptors, Virus</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Membrane Glycoproteins</term><term>Mutant Proteins</term><term>Peptidyl-Dipeptidase A</term><term>Receptors, Virus</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Peptidyl-Dipeptidase A</term><term>Receptors, Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN viral</term><term>Peptidyl-Dipeptidase A</term><term>Protéines mutantes</term><term>Récepteurs viraux</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Peptidyl-Dipeptidase A</term><term>Protéines de l'enveloppe virale</term><term>Protéines mutantes</term><term>Récepteurs viraux</term></keywords><keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Peptidyl-Dipeptidase A</term><term>Récepteurs viraux</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Amino Acid Substitution</term><term>Animals</term><term>Chiroptera</term><term>Disease Reservoirs</term><term>Genetic Variation</term><term>HeLa Cells</term><term>Humans</term><term>Models, Molecular</term><term>Molecular Sequence Data</term><term>Mutagenesis, Site-Directed</term><term>Protein Binding</term><term>Protein Structure, Tertiary</term><term>Sequence Alignment</term><term>Sequence Analysis, DNA</term><term>Spike Glycoprotein, Coronavirus</term><term>Virus Internalization</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Alignement de séquences</term><term>Analyse de séquence d'ADN</term><term>Animaux</term><term>Cellules HeLa</term><term>Chiroptera</term><term>Données de séquences moléculaires</term><term>Glycoprotéine de spicule des coronavirus</term><term>Humains</term><term>Liaison aux protéines</term><term>Modèles moléculaires</term><term>Mutagenèse dirigée</term><term>Pénétration virale</term><term>Réservoirs d'agents pathogènes</term><term>Structure tertiaire des protéines</term><term>Substitution d'acide aminé</term><term>Séquence d'acides aminés</term><term>Variation génétique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The discovery of SARS-like coronavirus in bats suggests that bats could be the natural reservoir of SARS-CoV. However, previous studies indicated the angiotensin-converting enzyme 2 (ACE2) protein, a known SARS-CoV receptor, from a horseshoe bat was unable to act as a functional receptor for SARS-CoV. Here, we extended our previous study to ACE2 molecules from seven additional bat species and tested their interactions with human SARS-CoV spike protein using both HIV-based pseudotype and live SARS-CoV infection assays. The results show that ACE2s of <italic>Myotis daubentoni</italic> and <italic>Rhinolophus sinicus</italic> support viral entry mediated by the SARS-CoV S protein, albeit with different efficiency in comparison to that of the human ACE2. Further, the alteration of several key residues either decreased or enhanced bat ACE2 receptor efficiency, as predicted from a structural modeling study of the different bat ACE2 molecules. These data suggest that <italic>M. daubentoni</italic> and <italic>R. sinicus</italic> are likely to be susceptible to SARS-CoV and may be candidates as the natural host of the SARS-CoV progenitor viruses. Furthermore, our current study also demonstrates that the genetic diversity of ACE2 among bats is greater than that observed among known SARS-CoV susceptible mammals, highlighting the possibility that there are many more uncharacterized bat species that can act as a reservoir of SARS-CoV or its progenitor viruses. This calls for continuation and expansion of field surveillance studies among different bat populations to eventually identify the true natural reservoir of SARS-CoV.</p><sec><title>Electronic supplementary material</title><p>The online version of this article (doi:10.1007/s00705-010-0729-6) contains supplementary material, which is available to authorized users.</p></sec></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Cui, J" uniqKey="Cui J">J Cui</name></author><author><name sortKey="Han, N" uniqKey="Han N">N Han</name></author><author><name sortKey="Streicker, D" uniqKey="Streicker D">D Streicker</name></author><author><name sortKey="Li, G" uniqKey="Li G">G Li</name></author><author><name sortKey="Tang, X" uniqKey="Tang X">X Tang</name></author><author><name sortKey="Shi, Z" uniqKey="Shi Z">Z Shi</name></author><author><name sortKey="Hu, Z" uniqKey="Hu Z">Z Hu</name></author><author><name sortKey="Zhao, G" uniqKey="Zhao G">G 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