Identification of a novel transcriptional repressor (HEPIS) that interacts with nsp-10 of SARS coronavirus.
Identifieur interne : 001C33 ( Ncbi/Curation ); précédent : 001C32; suivant : 001C34Identification of a novel transcriptional repressor (HEPIS) that interacts with nsp-10 of SARS coronavirus.
Auteurs : Min Hong [République populaire de Chine] ; Weizhong Li ; Lichun Wang ; Li Jiang ; Longding Liu ; Hongling Zhao ; Qihan LiSource :
- Viral immunology [ 0882-8245 ] ; 2008.
Descripteurs français
- KwdFr :
- ADN (métabolisme), Animaux, Cartographie d'interactions entre protéines, Chloramphenicol O-acetyltransferase (génétique), Cricetinae, Données de séquences moléculaires, Facteurs de transcription (métabolisme), Fusion artificielle de gènes, Gènes rapporteurs, Humains, Immunoprécipitation, Liaison aux protéines, Lignée cellulaire, Protéines de répression (métabolisme), Protéines nucléaires (métabolisme), Protéines virales non structurales (métabolisme), Régions promotrices (génétique), Séquence d'acides aminés, Séquence nucléotidique, Techniques de double hybride, Transcription génétique, Virus du SRAS (physiologie).
- MESH :
- génétique : Chloramphenicol O-acetyltransferase.
- métabolisme : ADN, Facteurs de transcription, Protéines de répression, Protéines nucléaires, Protéines virales non structurales.
- physiologie : Virus du SRAS.
- Animaux, Cartographie d'interactions entre protéines, Cricetinae, Données de séquences moléculaires, Fusion artificielle de gènes, Gènes rapporteurs, Humains, Immunoprécipitation, Liaison aux protéines, Lignée cellulaire, Régions promotrices (génétique), Séquence d'acides aminés, Séquence nucléotidique, Techniques de double hybride, Transcription génétique.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Artificial Gene Fusion, Base Sequence, Cell Line, Chloramphenicol O-Acetyltransferase (genetics), Cricetinae, DNA (metabolism), Genes, Reporter, Humans, Immunoprecipitation, Molecular Sequence Data, Nuclear Proteins (metabolism), Promoter Regions, Genetic, Protein Binding, Protein Interaction Mapping, Repressor Proteins (metabolism), SARS Virus (physiology), Transcription Factors (metabolism), Transcription, Genetic, Two-Hybrid System Techniques, Viral Nonstructural Proteins (metabolism).
- MESH :
- chemical , genetics : Chloramphenicol O-Acetyltransferase.
- chemical , metabolism : DNA, Nuclear Proteins, Repressor Proteins, Transcription Factors, Viral Nonstructural Proteins.
- physiology : SARS Virus.
- Amino Acid Sequence, Animals, Artificial Gene Fusion, Base Sequence, Cell Line, Cricetinae, Genes, Reporter, Humans, Immunoprecipitation, Molecular Sequence Data, Promoter Regions, Genetic, Protein Binding, Protein Interaction Mapping, Transcription, Genetic, Two-Hybrid System Techniques.
Abstract
A novel gene was previously isolated from a cDNA library of human embryo lung tissue by its encoded protein, which interacts with non-structural protein 10 (nsp-10) of the severe acute respiratory syndrome coronavirus (SARS-CoV). The protein was named human embryo lung cellular protein interacting with SARS-CoV nsp-10 (HEPIS), and it is composed of 147 amino acids with several CK II phosphorylation sites. In the present study, we demonstrated that HEPIS was capable of suppressing chloramphenicol acetyltransferase (CAT) gene expression controlled by different enhancerelements in a transcription assay. HEPIS interacted specifically with the HSP70 TATA sequence, and not with various other enhancer elements in a binding test. Furthermore, we co-immunoprecipitated HEPIS with BTF3, a component of the RNA pol II initiation complex, and observed reduced proliferation of HeLa cells transfected with the HEPIS gene. Taken together, our results suggest that HEPIS may function as a potential transcriptional repressor.
DOI: 10.1089/vim.2007.0108
PubMed: 18433331
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pubmed:18433331Le document en format XML
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<author><name sortKey="Wang, Lichun" sort="Wang, Lichun" uniqKey="Wang L" first="Lichun" last="Wang">Lichun Wang</name>
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<term>Artificial Gene Fusion</term>
<term>Base Sequence</term>
<term>Cell Line</term>
<term>Chloramphenicol O-Acetyltransferase (genetics)</term>
<term>Cricetinae</term>
<term>DNA (metabolism)</term>
<term>Genes, Reporter</term>
<term>Humans</term>
<term>Immunoprecipitation</term>
<term>Molecular Sequence Data</term>
<term>Nuclear Proteins (metabolism)</term>
<term>Promoter Regions, Genetic</term>
<term>Protein Binding</term>
<term>Protein Interaction Mapping</term>
<term>Repressor Proteins (metabolism)</term>
<term>SARS Virus (physiology)</term>
<term>Transcription Factors (metabolism)</term>
<term>Transcription, Genetic</term>
<term>Two-Hybrid System Techniques</term>
<term>Viral Nonstructural Proteins (metabolism)</term>
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<keywords scheme="KwdFr" xml:lang="fr"><term>ADN (métabolisme)</term>
<term>Animaux</term>
<term>Cartographie d'interactions entre protéines</term>
<term>Chloramphenicol O-acetyltransferase (génétique)</term>
<term>Cricetinae</term>
<term>Données de séquences moléculaires</term>
<term>Facteurs de transcription (métabolisme)</term>
<term>Fusion artificielle de gènes</term>
<term>Gènes rapporteurs</term>
<term>Humains</term>
<term>Immunoprécipitation</term>
<term>Liaison aux protéines</term>
<term>Lignée cellulaire</term>
<term>Protéines de répression (métabolisme)</term>
<term>Protéines nucléaires (métabolisme)</term>
<term>Protéines virales non structurales (métabolisme)</term>
<term>Régions promotrices (génétique)</term>
<term>Séquence d'acides aminés</term>
<term>Séquence nucléotidique</term>
<term>Techniques de double hybride</term>
<term>Transcription génétique</term>
<term>Virus du SRAS (physiologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Chloramphenicol O-Acetyltransferase</term>
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<term>Nuclear Proteins</term>
<term>Repressor Proteins</term>
<term>Transcription Factors</term>
<term>Viral Nonstructural Proteins</term>
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<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Chloramphenicol O-acetyltransferase</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ADN</term>
<term>Facteurs de transcription</term>
<term>Protéines de répression</term>
<term>Protéines nucléaires</term>
<term>Protéines virales non structurales</term>
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<keywords scheme="MESH" qualifier="physiologie" xml:lang="fr"><term>Virus du SRAS</term>
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<keywords scheme="MESH" qualifier="physiology" xml:lang="en"><term>SARS Virus</term>
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<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Animals</term>
<term>Artificial Gene Fusion</term>
<term>Base Sequence</term>
<term>Cell Line</term>
<term>Cricetinae</term>
<term>Genes, Reporter</term>
<term>Humans</term>
<term>Immunoprecipitation</term>
<term>Molecular Sequence Data</term>
<term>Promoter Regions, Genetic</term>
<term>Protein Binding</term>
<term>Protein Interaction Mapping</term>
<term>Transcription, Genetic</term>
<term>Two-Hybrid System Techniques</term>
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<term>Cartographie d'interactions entre protéines</term>
<term>Cricetinae</term>
<term>Données de séquences moléculaires</term>
<term>Fusion artificielle de gènes</term>
<term>Gènes rapporteurs</term>
<term>Humains</term>
<term>Immunoprécipitation</term>
<term>Liaison aux protéines</term>
<term>Lignée cellulaire</term>
<term>Régions promotrices (génétique)</term>
<term>Séquence d'acides aminés</term>
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<term>Techniques de double hybride</term>
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<front><div type="abstract" xml:lang="en">A novel gene was previously isolated from a cDNA library of human embryo lung tissue by its encoded protein, which interacts with non-structural protein 10 (nsp-10) of the severe acute respiratory syndrome coronavirus (SARS-CoV). The protein was named human embryo lung cellular protein interacting with SARS-CoV nsp-10 (HEPIS), and it is composed of 147 amino acids with several CK II phosphorylation sites. In the present study, we demonstrated that HEPIS was capable of suppressing chloramphenicol acetyltransferase (CAT) gene expression controlled by different enhancerelements in a transcription assay. HEPIS interacted specifically with the HSP70 TATA sequence, and not with various other enhancer elements in a binding test. Furthermore, we co-immunoprecipitated HEPIS with BTF3, a component of the RNA pol II initiation complex, and observed reduced proliferation of HeLa cells transfected with the HEPIS gene. Taken together, our results suggest that HEPIS may function as a potential transcriptional repressor.</div>
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