Severe acute respiratory syndrome coronavirus papain-like protease: structure of a viral deubiquitinating enzyme.
Identifieur interne : 001439 ( Ncbi/Curation ); précédent : 001438; suivant : 001440Severe acute respiratory syndrome coronavirus papain-like protease: structure of a viral deubiquitinating enzyme.
Auteurs : Kiira Ratia [États-Unis] ; Kumar Singh Saikatendu ; Bernard D. Santarsiero ; Naina Barretto ; Susan C. Baker ; Raymond C. Stevens ; Andrew D. MesecarSource :
- Proceedings of the National Academy of Sciences of the United States of America [ 0027-8424 ] ; 2006.
Descripteurs français
- KwdFr :
- MESH :
- enzymologie : Virus du SRAS.
- métabolisme : Papaïne, Peptide hydrolases, Ubiquitine.
- Cristallographie aux rayons X, Cysteine endopeptidases, Modèles moléculaires, Protéines virales, Sites de fixation, Structure secondaire des protéines.
English descriptors
- KwdEn :
- MESH :
- chemical , chemistry : Cysteine Endopeptidases, Viral Proteins.
- chemical , metabolism : Papain, Peptide Hydrolases, Ubiquitin.
- enzymology : SARS Virus.
- Binding Sites, Crystallography, X-Ray, Models, Molecular, Protein Structure, Secondary.
Abstract
Replication of severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) requires proteolytic processing of the replicase polyprotein by two viral cysteine proteases, a chymotrypsin-like protease (3CLpro) and a papain-like protease (PLpro). These proteases are important targets for development of antiviral drugs that would inhibit viral replication and reduce mortality associated with outbreaks of SARS-CoV. In this work, we describe the 1.85-A crystal structure of the catalytic core of SARS-CoV PLpro and show that the overall architecture adopts a fold closely resembling that of known deubiquitinating enzymes. Key features, however, distinguish PLpro from characterized deubiquitinating enzymes, including an intact zinc-binding motif, an unobstructed catalytically competent active site, and the presence of an intriguing, ubiquitin-like N-terminal domain. To gain insight into the active-site recognition of the C-terminal tail of ubiquitin and the related LXGG motif, we propose a model of PLpro in complex with ubiquitin-aldehyde that reveals well defined sites within the catalytic cleft that help to account for strict substrate-recognition motifs.
DOI: 10.1073/pnas.0510851103
PubMed: 16581910
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<term>Papain (metabolism)</term>
<term>Peptide Hydrolases (metabolism)</term>
<term>Protein Structure, Secondary</term>
<term>SARS Virus (enzymology)</term>
<term>Ubiquitin (metabolism)</term>
<term>Viral Proteins (chemistry)</term>
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<term>Modèles moléculaires</term>
<term>Papaïne (métabolisme)</term>
<term>Peptide hydrolases (métabolisme)</term>
<term>Protéines virales ()</term>
<term>Sites de fixation</term>
<term>Structure secondaire des protéines</term>
<term>Ubiquitine (métabolisme)</term>
<term>Virus du SRAS (enzymologie)</term>
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<term>Modèles moléculaires</term>
<term>Protéines virales</term>
<term>Sites de fixation</term>
<term>Structure secondaire des protéines</term>
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<front><div type="abstract" xml:lang="en">Replication of severe acute respiratory syndrome (SARS) coronavirus (SARS-CoV) requires proteolytic processing of the replicase polyprotein by two viral cysteine proteases, a chymotrypsin-like protease (3CLpro) and a papain-like protease (PLpro). These proteases are important targets for development of antiviral drugs that would inhibit viral replication and reduce mortality associated with outbreaks of SARS-CoV. In this work, we describe the 1.85-A crystal structure of the catalytic core of SARS-CoV PLpro and show that the overall architecture adopts a fold closely resembling that of known deubiquitinating enzymes. Key features, however, distinguish PLpro from characterized deubiquitinating enzymes, including an intact zinc-binding motif, an unobstructed catalytically competent active site, and the presence of an intriguing, ubiquitin-like N-terminal domain. To gain insight into the active-site recognition of the C-terminal tail of ubiquitin and the related LXGG motif, we propose a model of PLpro in complex with ubiquitin-aldehyde that reveals well defined sites within the catalytic cleft that help to account for strict substrate-recognition motifs.</div>
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