Genosensor on gold films with enzymatic electrochemical detection of a SARS virus sequence☆
Identifieur interne : 000E67 ( Ncbi/Curation ); précédent : 000E66; suivant : 000E68Genosensor on gold films with enzymatic electrochemical detection of a SARS virus sequence☆
Auteurs : Patricia Abad-Valle ; M. Teresa Fernández-Abedul ; Agustín Costa-GarcíaSource :
- Biosensors & Bioelectronics [ 0956-5663 ] ; 2004.
Descripteurs français
- KwdFr :
- ADN viral (analyse), ADN viral (génétique), Analyse de panne d'appareillage, Analyse de séquence d'ADN (), Analyse de séquence d'ADN (instrumentation), Conception d'appareillage, Hybridation in situ (), Hybridation in situ (instrumentation), Or (), Techniques de biocapteur (), Techniques de biocapteur (instrumentation), Virus du SRAS (génétique), Virus du SRAS (isolement et purification), Électrochimie (), Électrochimie (instrumentation).
- MESH :
- analyse : ADN viral.
- génétique : ADN viral, Virus du SRAS.
- isolement et purification : Virus du SRAS.
- Analyse de panne d'appareillage, Analyse de séquence d'ADN, Conception d'appareillage, Hybridation in situ, Or, Techniques de biocapteur, Électrochimie.
English descriptors
- KwdEn :
- Biosensing Techniques (instrumentation), Biosensing Techniques (methods), DNA, Viral (analysis), DNA, Viral (genetics), Electrochemistry (instrumentation), Electrochemistry (methods), Equipment Design, Equipment Failure Analysis, Gold (chemistry), In Situ Hybridization (instrumentation), In Situ Hybridization (methods), SARS Virus (genetics), SARS Virus (isolation & purification), Sequence Analysis, DNA (instrumentation), Sequence Analysis, DNA (methods).
- MESH :
- chemical , analysis : DNA, Viral.
- chemical , chemistry : Gold.
- chemical , genetics : DNA, Viral.
- genetics : SARS Virus.
- instrumentation : Biosensing Techniques, Electrochemistry, In Situ Hybridization, Sequence Analysis, DNA.
- isolation & purification : SARS Virus.
- methods : Biosensing Techniques, Electrochemistry, In Situ Hybridization, Sequence Analysis, DNA.
- Equipment Design, Equipment Failure Analysis.
Abstract
A hybridisation-based genosensor was designed on a 100 nm sputtered gold film. This material worked as an immobilisation and transduction surface. A 30-mer sequence that encodes a short lysine-rich region, unique to SARS (severe acute respiratory syndrome) virus, was chosen as target. A complementary strand (probe), labelled with a thiol group at the 3′-end, was immobilised on the film. After blocking the surface, hybridisation with the biotin-conjugated SARS strand (at the 3′-end) took place. Interaction with alkaline phosphatase-labelled streptavidin permits amplified indirect electrochemical detection. The analytical signal is constituted by an electrochemical process of indigo carmine, the soluble product of the enzymatic hydrolysis of 3-indoxyl phosphate. The use of a sensitive electrochemical technique such as square wave voltammetry allowed a detection limit of 6 pM to be obtained for this DNA sequence, lower than any other found in the bibliography. The parameters affecting the methodology were studied, with special attention being placed on selectivity. Specificity was clearly enhanced when interaction time and stringency (in the form of formamide percentage) were increased. With 1 h of strand interaction and employing 50% of formamide in the hybridisation buffer, a 3-base mismatch strand was perfectly distinguished from the complementary.
Url:
DOI: 10.1016/j.bios.2004.10.019
PubMed: 15797323
PubMed Central: 7126974
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :000E58
- to stream Pmc, to step Curation: Pour aller vers cette notice dans l'étape Curation :000E58
- to stream Pmc, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :001653
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :002815
- to stream PubMed, to step Curation: Pour aller vers cette notice dans l'étape Curation :002815
- to stream PubMed, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :002694
- to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :000E67
Links to Exploration step
PMC:7126974Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Genosensor on gold films with enzymatic electrochemical detection of a SARS virus sequence<sup><xref ref-type="fn" rid="d32e1184">☆</xref></sup></title><author><name sortKey="Abad Valle, Patricia" sort="Abad Valle, Patricia" uniqKey="Abad Valle P" first="Patricia" last="Abad-Valle">Patricia Abad-Valle</name></author><author><name sortKey="Fernandez Abedul, M Teresa" sort="Fernandez Abedul, M Teresa" uniqKey="Fernandez Abedul M" first="M. Teresa" last="Fernández-Abedul">M. Teresa Fernández-Abedul</name></author><author><name sortKey="Costa Garcia, Agustin" sort="Costa Garcia, Agustin" uniqKey="Costa Garcia A" first="Agustín" last="Costa-García">Agustín Costa-García</name></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">15797323</idno><idno type="pmc">7126974</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7126974</idno><idno type="RBID">PMC:7126974</idno><idno type="doi">10.1016/j.bios.2004.10.019</idno><date when="2004">2004</date><idno type="wicri:Area/Pmc/Corpus">000E58</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000E58</idno><idno type="wicri:Area/Pmc/Curation">000E58</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000E58</idno><idno type="wicri:Area/Pmc/Checkpoint">001653</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">001653</idno><idno type="wicri:source">PubMed</idno><idno type="RBID">pubmed:15797323</idno><idno type="wicri:Area/PubMed/Corpus">002815</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002815</idno><idno type="wicri:Area/PubMed/Curation">002815</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002815</idno><idno type="wicri:Area/PubMed/Checkpoint">002694</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002694</idno><idno type="wicri:Area/Ncbi/Merge">000E67</idno><idno type="wicri:Area/Ncbi/Curation">000E67</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Genosensor on gold films with enzymatic electrochemical detection of a SARS virus sequence<sup><xref ref-type="fn" rid="d32e1184">☆</xref></sup></title><author><name sortKey="Abad Valle, Patricia" sort="Abad Valle, Patricia" uniqKey="Abad Valle P" first="Patricia" last="Abad-Valle">Patricia Abad-Valle</name></author><author><name sortKey="Fernandez Abedul, M Teresa" sort="Fernandez Abedul, M Teresa" uniqKey="Fernandez Abedul M" first="M. Teresa" last="Fernández-Abedul">M. Teresa Fernández-Abedul</name></author><author><name sortKey="Costa Garcia, Agustin" sort="Costa Garcia, Agustin" uniqKey="Costa Garcia A" first="Agustín" last="Costa-García">Agustín Costa-García</name></author></analytic><series><title level="j">Biosensors & Bioelectronics</title><idno type="ISSN">0956-5663</idno><idno type="eISSN">1873-4235</idno><imprint><date when="2004">2004</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Biosensing Techniques (instrumentation)</term><term>Biosensing Techniques (methods)</term><term>DNA, Viral (analysis)</term><term>DNA, Viral (genetics)</term><term>Electrochemistry (instrumentation)</term><term>Electrochemistry (methods)</term><term>Equipment Design</term><term>Equipment Failure Analysis</term><term>Gold (chemistry)</term><term>In Situ Hybridization (instrumentation)</term><term>In Situ Hybridization (methods)</term><term>SARS Virus (genetics)</term><term>SARS Virus (isolation & purification)</term><term>Sequence Analysis, DNA (instrumentation)</term><term>Sequence Analysis, DNA (methods)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ADN viral (analyse)</term><term>ADN viral (génétique)</term><term>Analyse de panne d'appareillage</term><term>Analyse de séquence d'ADN ()</term><term>Analyse de séquence d'ADN (instrumentation)</term><term>Conception d'appareillage</term><term>Hybridation in situ ()</term><term>Hybridation in situ (instrumentation)</term><term>Or ()</term><term>Techniques de biocapteur ()</term><term>Techniques de biocapteur (instrumentation)</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (isolement et purification)</term><term>Électrochimie ()</term><term>Électrochimie (instrumentation)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>DNA, Viral</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" xml:lang="en"><term>Gold</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>DNA, Viral</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ADN viral</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ADN viral</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="instrumentation" xml:lang="en"><term>Biosensing Techniques</term><term>Electrochemistry</term><term>In Situ Hybridization</term><term>Sequence Analysis, DNA</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Biosensing Techniques</term><term>Electrochemistry</term><term>In Situ Hybridization</term><term>Sequence Analysis, DNA</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Equipment Design</term><term>Equipment Failure Analysis</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de panne d'appareillage</term><term>Analyse de séquence d'ADN</term><term>Conception d'appareillage</term><term>Hybridation in situ</term><term>Or</term><term>Techniques de biocapteur</term><term>Électrochimie</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>A hybridisation-based genosensor was designed on a 100 nm sputtered gold film. This material worked as an immobilisation and transduction surface. A 30-mer sequence that encodes a short lysine-rich region, unique to SARS (severe acute respiratory syndrome) virus, was chosen as target. A complementary strand (probe), labelled with a thiol group at the 3′-end, was immobilised on the film. After blocking the surface, hybridisation with the biotin-conjugated SARS strand (at the 3′-end) took place. Interaction with alkaline phosphatase-labelled streptavidin permits amplified indirect electrochemical detection. The analytical signal is constituted by an electrochemical process of indigo carmine, the soluble product of the enzymatic hydrolysis of 3-indoxyl phosphate. The use of a sensitive electrochemical technique such as square wave voltammetry allowed a detection limit of 6 pM to be obtained for this DNA sequence, lower than any other found in the bibliography. The parameters affecting the methodology were studied, with special attention being placed on selectivity. Specificity was clearly enhanced when interaction time and stringency (in the form of formamide percentage) were increased. With 1 h of strand interaction and employing 50% of formamide in the hybridisation buffer, a 3-base mismatch strand was perfectly distinguished from the complementary.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Abad Villar, E M" uniqKey="Abad Villar E">E.M. Abad-Villar</name></author><author><name sortKey="Fernandez Abedul, M T" uniqKey="Fernandez Abedul M">M.T. Fernández-Abedul</name></author><author><name sortKey="Costa Garcia, A" uniqKey="Costa Garcia A">A. Costa-García</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Abad Villar, E M" uniqKey="Abad Villar E">E.M. Abad-Villar</name></author><author><name sortKey="Fernandez Abedul, M T" uniqKey="Fernandez Abedul M">M.T. Fernández-Abedul</name></author><author><name sortKey="Costa Garcia, A" uniqKey="Costa Garcia A">A. Costa-García</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Abad Villar, E M" uniqKey="Abad Villar E">E.M. Abad-Villar</name></author><author><name sortKey="Fernandez Abedul, M T" uniqKey="Fernandez Abedul M">M.T. Fernández-Abedul</name></author><author><name sortKey="Costa Garcia, A" uniqKey="Costa Garcia A">A. Costa-García</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Adams, R N" uniqKey="Adams R">R.N. Adams</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Aguilar, Z P" uniqKey="Aguilar Z">Z.P. Aguilar</name></author><author><name sortKey="Ritsch, I" uniqKey="Ritsch I">I. Ritsch</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Alfonta, L" uniqKey="Alfonta L">L. Alfonta</name></author><author><name sortKey="Singh, A K" uniqKey="Singh A">A.K. Singh</name></author><author><name sortKey="Willner, I" uniqKey="Willner I">I. Willner</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bain, C D" uniqKey="Bain C">C.D. Bain</name></author><author><name sortKey="Troughton, E B" uniqKey="Troughton E">E.B. Troughton</name></author><author><name sortKey="Tao, Y T" uniqKey="Tao Y">Y.-T. Tao</name></author><author><name sortKey="Evall, J" uniqKey="Evall J">J. Evall</name></author><author><name sortKey="Whitesides, G M" uniqKey="Whitesides G">G.M. Whitesides</name></author><author><name sortKey="Nuzzo, R G" uniqKey="Nuzzo R">R.G. Nuzzo</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bengoechea Lvarez, M J" uniqKey="Bengoechea Lvarez M">M.J. Bengoechea-Álvarez</name></author><author><name sortKey="Fernandez Abedul, M T" uniqKey="Fernandez Abedul M">M.T. Fernández-Abedul</name></author><author><name sortKey="Costa Garcia, A" uniqKey="Costa Garcia A">A. Costa-García</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Brett, A M O" uniqKey="Brett A">A.M.O. Brett</name></author><author><name sortKey="Matysik, F M" uniqKey="Matysik F">F.-M. Matysik</name></author><author><name sortKey="Vieira, M T" uniqKey="Vieira M">M.T. Vieira</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Campas, M" uniqKey="Campas M">M. Campas</name></author><author><name sortKey="Katakis, I" uniqKey="Katakis I">I. Katakis</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Carpini, G" uniqKey="Carpini G">G. Carpini</name></author><author><name sortKey="Lucarelli, F" uniqKey="Lucarelli F">F. Lucarelli</name></author><author><name sortKey="Marrazza, G" uniqKey="Marrazza G">G. Marrazza</name></author><author><name sortKey="Mascini, M" uniqKey="Mascini M">M. Mascini</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Casta O Lvarez, M" uniqKey="Casta O Lvarez M">M. Castaño-Álvarez</name></author><author><name sortKey="Fernandez Abedul, M T" uniqKey="Fernandez Abedul M">M.T. Fernández-Abedul</name></author><author><name sortKey="Costa Garcia, A" uniqKey="Costa Garcia A">A. Costa-García</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="De Los Santos Alvarez, P" uniqKey="De Los Santos Alvarez P">P. De-Los-Santos-Alvarez</name></author><author><name sortKey="Lobo Castanon, M J" uniqKey="Lobo Castanon M">M.J. Lobo-Castanon</name></author><author><name sortKey="Miranda Ordieres, A J" uniqKey="Miranda Ordieres A">A.J. Miranda-Ordieres</name></author><author><name sortKey="Tu N Blanco, P" uniqKey="Tu N Blanco P">P. Tuñón-Blanco</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Diaz Gonzalez, M" uniqKey="Diaz Gonzalez M">M. Díaz-González</name></author><author><name sortKey="Fernandez Sanchez, C" uniqKey="Fernandez Sanchez C">C. Fernández-Sánchez</name></author><author><name sortKey="Costa Garcia, A" uniqKey="Costa Garcia A">A. Costa-García</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dominguez, E" uniqKey="Dominguez E">E. Domínguez</name></author><author><name sortKey="Rinc N, O" uniqKey="Rinc N O">O. Rincón</name></author><author><name sortKey="Narvaez, A" uniqKey="Narvaez A">A. Narváez</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Duan, C" uniqKey="Duan C">C. Duan</name></author><author><name sortKey="Meyerhoff, M E" uniqKey="Meyerhoff M">M.E. Meyerhoff</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Fernandez Sanchez, C" uniqKey="Fernandez Sanchez C">C. Fernández-Sánchez</name></author><author><name sortKey="Costa Garcia, A" uniqKey="Costa Garcia A">A. Costa-García</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Gherghi, I Ch" uniqKey="Gherghi I">I.Ch. Gherghi</name></author><author><name sortKey="Girousi, S Th" uniqKey="Girousi S">S.Th. Girousi</name></author><author><name sortKey="Voulgaropoulos, A" uniqKey="Voulgaropoulos A">A. Voulgaropoulos</name></author><author><name sortKey="Tzimou Tsitouridou, R" uniqKey="Tzimou Tsitouridou R">R. Tzimou-Tsitouridou</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Herne, T M" uniqKey="Herne T">T.M. Herne</name></author><author><name sortKey="Tarlov, M J" uniqKey="Tarlov M">M.J. Tarlov</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jenkins, S H" uniqKey="Jenkins S">S.H. Jenkins</name></author><author><name sortKey="Heineman, W R" uniqKey="Heineman W">W.R. Heineman</name></author><author><name sortKey="Halsall, H B" uniqKey="Halsall H">H.B. Halsall</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Jordan, C E" uniqKey="Jordan C">C.E. Jordan</name></author><author><name sortKey="Frutos, A G" uniqKey="Frutos A">A.G. Frutos</name></author><author><name sortKey="Thiel, A J" uniqKey="Thiel A">A.J. Thiel</name></author><author><name sortKey="Corn, R M" uniqKey="Corn R">R.M. Corn</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kelley, S O" uniqKey="Kelley S">S.O. Kelley</name></author><author><name sortKey="Boon, E M" uniqKey="Boon E">E.M. Boon</name></author><author><name sortKey="Barton, J K" uniqKey="Barton J">J.K. Barton</name></author><author><name sortKey="Jakson, N M" uniqKey="Jakson N">N.M. Jakson</name></author><author><name sortKey="Hill, M G" uniqKey="Hill M">M.G. Hill</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Levicky, R" uniqKey="Levicky R">R. Levicky</name></author><author><name sortKey="Herne, T M" uniqKey="Herne T">T.M. Herne</name></author><author><name sortKey="Tarlow, M J" uniqKey="Tarlow M">M.J. Tarlow</name></author><author><name sortKey="Satija, S K" uniqKey="Satija S">S.K. Satija</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lucarelli, F" uniqKey="Lucarelli F">F. Lucarelli</name></author><author><name sortKey="Marrazza, G" uniqKey="Marrazza G">G. Marrazza</name></author><author><name sortKey="Turner, A P F" uniqKey="Turner A">A.P.F. Turner</name></author><author><name sortKey="Marcini, M" uniqKey="Marcini M">M. Marcini</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Marra, M A" uniqKey="Marra M">M.A. Marra</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Matisyk, F M" uniqKey="Matisyk F">F.-M. Matisyk</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Meusel, M" uniqKey="Meusel M">M. Meusel</name></author><author><name sortKey="Renneberg, R" uniqKey="Renneberg R">R. Renneberg</name></author><author><name sortKey="Spener, F" uniqKey="Spener F">F. Spener</name></author><author><name sortKey="Schmitz, G" uniqKey="Schmitz G">G. Schmitz</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Minunni, M" uniqKey="Minunni M">M. Minunni</name></author><author><name sortKey="Tombelli, S" uniqKey="Tombelli S">S. Tombelli</name></author><author><name sortKey="Scielzi, R" uniqKey="Scielzi R">R. Scielzi</name></author><author><name sortKey="Mannelli, I" uniqKey="Mannelli I">I. Mannelli</name></author><author><name sortKey="Mascini, M" uniqKey="Mascini M">M. Mascini</name></author><author><name sortKey="Gaudiano, C" uniqKey="Gaudiano C">C. Gaudiano</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Nakamura, H" uniqKey="Nakamura H">H. Nakamura</name></author><author><name sortKey="Karube, I" uniqKey="Karube I">I. Karube</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pan, W" uniqKey="Pan W">W. Pan</name></author><author><name sortKey="Durning, C J" uniqKey="Durning C">C.J. Durning</name></author><author><name sortKey="Turro, N J" uniqKey="Turro N">N.J. Turro</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pividori, M I" uniqKey="Pividori M">M.I. Pividori</name></author><author><name sortKey="Merkoci, A" uniqKey="Merkoci A">A. Merkoçi</name></author><author><name sortKey="Alegret, S" uniqKey="Alegret S">S. Alegret</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Pividori, M I" uniqKey="Pividori M">M.I. Pividori</name></author><author><name sortKey="Merkoci" uniqKey="Merkoci">Merkoçi</name></author><author><name sortKey="Alegret, S" uniqKey="Alegret S">S. Alegret</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Steel, A B" uniqKey="Steel A">A.B. Steel</name></author><author><name sortKey="Herne, T M" uniqKey="Herne T">T.M. Herne</name></author><author><name sortKey="Tarlov, M J" uniqKey="Tarlov M">M.J. Tarlov</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Taft, B J" uniqKey="Taft B">B.J. Taft</name></author><author><name sortKey="O Eefe, M" uniqKey="O Eefe M">M. O’Keefe</name></author><author><name sortKey="Fourkas, J T" uniqKey="Fourkas J">J.T. Fourkas</name></author><author><name sortKey="Kelley, S O" uniqKey="Kelley S">S.O. Kelley</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Tie, F" uniqKey="Tie F">F. Tie</name></author><author><name sortKey="Pan, A" uniqKey="Pan A">A. Pan</name></author><author><name sortKey="Ru, B" uniqKey="Ru B">B. Ru</name></author><author><name sortKey="Wang, W" uniqKey="Wang W">W. Wang</name></author><author><name sortKey="Hu, Y" uniqKey="Hu Y">Y. Hu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wang, J" uniqKey="Wang J">J. Wang</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wijayawardhana, C A" uniqKey="Wijayawardhana C">C.A. Wijayawardhana</name></author><author><name sortKey="Halsall, H B" uniqKey="Halsall H">H.B. Halsall</name></author><author><name sortKey="Heineman, W R" uniqKey="Heineman W">W.R. Heineman</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Wu, K" uniqKey="Wu K">K. Wu</name></author><author><name sortKey="Fei, J" uniqKey="Fei J">J. Fei</name></author><author><name sortKey="Bai, J" uniqKey="Bai J">J. Bai</name></author><author><name sortKey="Hu, S" uniqKey="Hu S">S. Hu</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Xu, D" uniqKey="Xu D">D. Xu</name></author><author><name sortKey="Huang, K" uniqKey="Huang K">K. Huang</name></author><author><name sortKey="Liu, Z" uniqKey="Liu Z">Z. Liu</name></author><author><name sortKey="Liu, Y" uniqKey="Liu Y">Y. Liu</name></author><author><name sortKey="Ma, L" uniqKey="Ma L">L. Ma</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Zhu, N" uniqKey="Zhu N">N. Zhu</name></author><author><name sortKey="Cai, H" uniqKey="Cai H">H. Cai</name></author><author><name sortKey="He, P" uniqKey="He P">P. He</name></author><author><name sortKey="Fang, Y" uniqKey="Fang Y">Y. Fang</name></author></analytic></biblStruct></listBibl></div1></back></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Ncbi/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000E67 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd -nk 000E67 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Ncbi
|étape= Curation
|type= RBID
|clé= PMC:7126974
|texte= Genosensor on gold films with enzymatic electrochemical detection of a SARS virus sequence☆
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/RBID.i -Sk "pubmed:15797323" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |