Evaluation of a recombinant nucleocapsid protein-based assay for anti-SARS-CoV IgG detection.
Identifieur interne : 000C58 ( Ncbi/Curation ); précédent : 000C57; suivant : 000C59Evaluation of a recombinant nucleocapsid protein-based assay for anti-SARS-CoV IgG detection.
Auteurs : Paul K S. Chan [République populaire de Chine] ; Esther Y M. Liu ; Danny T M. Leung ; Jo L K. Cheung ; C H Ma ; Frankie C H. Tam ; Mamie Hui ; John S. Tam ; Pak Leong LimSource :
- Journal of medical virology [ 0146-6615 ] ; 2005.
Descripteurs français
- KwdFr :
- Adolescent, Adulte, Anticorps antiviraux (sang), Enfant, Enfant d'âge préscolaire, Femelle, Humains, Immunoglobuline G (sang), Mâle, Protéines nucléocapside (immunologie), Protéines recombinantes (immunologie), Sensibilité et spécificité, Syndrome respiratoire aigu sévère (immunologie), Test ELISA (), Virus du SRAS (immunologie).
- MESH :
English descriptors
- KwdEn :
- Adolescent, Adult, Antibodies, Viral (blood), Child, Child, Preschool, Enzyme-Linked Immunosorbent Assay (methods), Female, Humans, Immunoglobulin G (blood), Male, Nucleocapsid Proteins (immunology), Recombinant Proteins (immunology), SARS Virus (immunology), Sensitivity and Specificity, Severe Acute Respiratory Syndrome (immunology).
- MESH :
- chemical , blood : Antibodies, Viral, Immunoglobulin G.
- chemical , immunology : Nucleocapsid Proteins, Recombinant Proteins.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome.
- methods : Enzyme-Linked Immunosorbent Assay.
- Adolescent, Adult, Child, Child, Preschool, Female, Humans, Male, Sensitivity and Specificity.
Abstract
A high throughput accurate assay for anti-SARS-CoV IgG detection is needed for large-scale epidemiological studies. The evaluation of a commercial recombinant nucleocapsid protein-based microtitre plate enzyme immunoassay, ELISARS is described. The results on 150 sera from SARS patients and 450 sera from non-SARS controls showed that this assay had a high level of sensitivity (96.2% for late serum samples) and specificity (97.8%). The performance and setup of this assay fulfills the requirement as a screening test for large-scale studies. A vast majority of SARS patients developed antibodies against the nucleocapsid protein. In some patients (10/45), a high level of anti-nucleocapsid antibody appeared very early in the course of the illness. In contrast, a minority (4 of 105 patients) never developed these antibodies. The implication of differences in antibody response to the nucleocapsid protein deserves further investigation.
DOI: 10.1002/jmv.20254
PubMed: 15602743
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pubmed:15602743Le document en format XML
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<term>Child, Preschool</term>
<term>Enzyme-Linked Immunosorbent Assay (methods)</term>
<term>Female</term>
<term>Humans</term>
<term>Immunoglobulin G (blood)</term>
<term>Male</term>
<term>Nucleocapsid Proteins (immunology)</term>
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<term>SARS Virus (immunology)</term>
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<term>Sensibilité et spécificité</term>
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<term>Virus du SRAS</term>
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<term>Severe Acute Respiratory Syndrome</term>
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<front><div type="abstract" xml:lang="en">A high throughput accurate assay for anti-SARS-CoV IgG detection is needed for large-scale epidemiological studies. The evaluation of a commercial recombinant nucleocapsid protein-based microtitre plate enzyme immunoassay, ELISARS is described. The results on 150 sera from SARS patients and 450 sera from non-SARS controls showed that this assay had a high level of sensitivity (96.2% for late serum samples) and specificity (97.8%). The performance and setup of this assay fulfills the requirement as a screening test for large-scale studies. A vast majority of SARS patients developed antibodies against the nucleocapsid protein. In some patients (10/45), a high level of anti-nucleocapsid antibody appeared very early in the course of the illness. In contrast, a minority (4 of 105 patients) never developed these antibodies. The implication of differences in antibody response to the nucleocapsid protein deserves further investigation.</div>
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