Severe acute respiratory syndrome coronavirus spike protein expressed by attenuated vaccinia virus protectively immunizes mice
Identifieur interne : 000763 ( Ncbi/Curation ); précédent : 000762; suivant : 000764Severe acute respiratory syndrome coronavirus spike protein expressed by attenuated vaccinia virus protectively immunizes mice
Auteurs : Himani Bisht ; Anjeanette Roberts [États-Unis] ; Leatrice Vogel [États-Unis] ; Alexander Bukreyev [États-Unis] ; Peter L. Collins [États-Unis] ; Brian R. Murphy [États-Unis] ; Kanta Subbarao [États-Unis] ; Bernard MossSource :
- Proceedings of the National Academy of Sciences of the United States of America [ 0027-8424 ] ; 2004.
Descripteurs français
- KwdFr :
- Animaux, Cellules HeLa, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (immunologie), Glycoprotéines membranaires (physiologie), Humains, Lignée cellulaire, Microscopie confocale, Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (immunologie), Protéines de l'enveloppe virale (physiologie), Protéines recombinantes (génétique), Protéines recombinantes (immunologie), Souris, Souris de lignée BALB C, Virus de la vaccine (génétique), Virus du SRAS (physiologie).
- MESH :
- génétique : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines recombinantes, Virus de la vaccine.
- immunologie : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines recombinantes.
- physiologie : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Virus du SRAS.
- Animaux, Cellules HeLa, Glycoprotéine de spicule des coronavirus, Humains, Lignée cellulaire, Microscopie confocale, Souris, Souris de lignée BALB C.
English descriptors
- KwdEn :
- Animals, Cell Line, HeLa Cells, Humans, Membrane Glycoproteins (genetics), Membrane Glycoproteins (immunology), Membrane Glycoproteins (physiology), Mice, Mice, Inbred BALB C, Microscopy, Confocal, Recombinant Proteins (genetics), Recombinant Proteins (immunology), SARS Virus (physiology), Spike Glycoprotein, Coronavirus, Vaccinia virus (genetics), Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology), Viral Envelope Proteins (physiology).
- MESH :
- chemical , genetics : Membrane Glycoproteins, Recombinant Proteins, Viral Envelope Proteins.
- chemical , immunology : Membrane Glycoproteins, Recombinant Proteins, Viral Envelope Proteins.
- chemical , physiology : Membrane Glycoproteins, Viral Envelope Proteins.
- genetics : Vaccinia virus.
- physiology : SARS Virus.
- Animals, Cell Line, HeLa Cells, Humans, Mice, Mice, Inbred BALB C, Microscopy, Confocal, Spike Glycoprotein, Coronavirus.
Abstract
The spike protein (S), a membrane component of severe acute respiratory syndrome coronavirus (SARS-CoV) is anticipated to be an important component of candidate vaccines. We constructed recombinant forms of the highly attenuated modified vaccinia virus Ankara (MVA) containing the gene encoding full-length SARS-CoV S with and without a C-terminal epitope tag called MVA/S-HA and MVA/S, respectively. Cells infected with MVA/Sor MVA/S-HA synthesized a 200-kDa protein, which was recognized by antibody raised against a synthetic peptide of SARS-CoV S or the epitope tag in Western blot analyses. Further studies indicated that S was N
Url:
DOI: 10.1073/pnas.0401939101
PubMed: 15096611
PubMed Central: 404098
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PMC:404098Le document en format XML
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<series><title level="j">Proceedings of the National Academy of Sciences of the United States of America</title>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Cell Line</term>
<term>HeLa Cells</term>
<term>Humans</term>
<term>Membrane Glycoproteins (genetics)</term>
<term>Membrane Glycoproteins (immunology)</term>
<term>Membrane Glycoproteins (physiology)</term>
<term>Mice</term>
<term>Mice, Inbred BALB C</term>
<term>Microscopy, Confocal</term>
<term>Recombinant Proteins (genetics)</term>
<term>Recombinant Proteins (immunology)</term>
<term>SARS Virus (physiology)</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Vaccinia virus (genetics)</term>
<term>Viral Envelope Proteins (genetics)</term>
<term>Viral Envelope Proteins (immunology)</term>
<term>Viral Envelope Proteins (physiology)</term>
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<term>Cellules HeLa</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires (génétique)</term>
<term>Glycoprotéines membranaires (immunologie)</term>
<term>Glycoprotéines membranaires (physiologie)</term>
<term>Humains</term>
<term>Lignée cellulaire</term>
<term>Microscopie confocale</term>
<term>Protéines de l'enveloppe virale (génétique)</term>
<term>Protéines de l'enveloppe virale (immunologie)</term>
<term>Protéines de l'enveloppe virale (physiologie)</term>
<term>Protéines recombinantes (génétique)</term>
<term>Protéines recombinantes (immunologie)</term>
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<term>Souris de lignée BALB C</term>
<term>Virus de la vaccine (génétique)</term>
<term>Virus du SRAS (physiologie)</term>
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<term>Viral Envelope Proteins</term>
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<term>Recombinant Proteins</term>
<term>Viral Envelope Proteins</term>
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<keywords scheme="MESH" type="chemical" qualifier="physiology" xml:lang="en"><term>Membrane Glycoproteins</term>
<term>Viral Envelope Proteins</term>
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<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glycoprotéines membranaires</term>
<term>Protéines de l'enveloppe virale</term>
<term>Protéines recombinantes</term>
<term>Virus de la vaccine</term>
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<term>Protéines de l'enveloppe virale</term>
<term>Protéines recombinantes</term>
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<term>Protéines de l'enveloppe virale</term>
<term>Virus du SRAS</term>
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<front><div type="abstract" xml:lang="en"><p>The spike protein (S), a membrane component of severe acute respiratory syndrome coronavirus (SARS-CoV) is anticipated to be an important component of candidate vaccines. We constructed recombinant forms of the highly attenuated modified vaccinia virus Ankara (MVA) containing the gene encoding full-length SARS-CoV S with and without a C-terminal epitope tag called MVA/S-HA and MVA/S, respectively. Cells infected with MVA/Sor MVA/S-HA synthesized a 200-kDa protein, which was recognized by antibody raised against a synthetic peptide of SARS-CoV S or the epitope tag in Western blot analyses. Further studies indicated that S was N<italic>-</italic>
glycosylated and migrated in SDS polyacrylamide gels with an apparent mass of ≈160 kDa after treatment with peptide N-glycosidase F. The acquisition of resistance to endoglycosidase H indicated trafficking of S to the medial Golgi compartment, and confocal microscopy showed that S was transported to the cell surface. Intranasal or intramuscular inoculations of BALB/c mice with MVA/S produced serum antibodies that recognized the SARS S in ELISA and neutralized SARS-CoV <italic>in vitro</italic>
. Moreover, MVA/S administered by either route elicited protective immunity, as shown by reduced titers of SARS-CoV in the upper and lower respiratory tracts of mice after challenge. Passive transfer of serum from mice immunized with MVA/S to naïve mice also reduced the replication of SARS-CoV in the respiratory tract after challenge, demonstrating a role for antibody to S in protection. The attenuated nature of MVA and the ability of MVA/S to induce neutralizing antibody that protects mice support further development of this candidate vaccine.</p>
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