Silencing SARS‐CoV Spike protein expression in cultured cells by RNA interference
Identifieur interne : 000629 ( Ncbi/Curation ); précédent : 000628; suivant : 000630Silencing SARS‐CoV Spike protein expression in cultured cells by RNA interference
Auteurs : Yuanjiang Zhang ; Tieshi Li ; Ling Fu ; Changming Yu ; Yinghua Li ; Xialian Xu ; Yinyin Wang ; Hongxiu Ning ; Shuping Zhang ; Wei Chen ; Lorne A. Babiuk ; Zhijie ChangSource :
- Febs Letters [ 0014-5793 ] ; 2004.
Descripteurs français
- KwdFr :
- ARN messager (métabolisme), ARN viral (isolement et purification), Animaux, Cellules Vero, Coronavirus (génétique), Expression des gènes, Extinction de l'expression des gènes, Humains, Hémagglutinines virales (métabolisme), Interférence par ARN, Lignée cellulaire, Petit ARN interférent (génétique), Protéines virales structurales (génétique), Protéines virales structurales (métabolisme), Rein (cytologie), Rein (embryologie), Syndrome respiratoire aigu sévère (), Transformation cellulaire virale, Vecteurs génétiques, Virus du SRAS (génétique), microARN (génétique).
- MESH :
- cytologie : Rein.
- embryologie : Rein.
- génétique : Coronavirus, Petit ARN interférent, Protéines virales structurales, Virus du SRAS, microARN.
- isolement et purification : ARN viral.
- métabolisme : ARN messager, Hémagglutinines virales, Protéines virales structurales.
- Animaux, Cellules Vero, Expression des gènes, Extinction de l'expression des gènes, Humains, Interférence par ARN, Lignée cellulaire, Syndrome respiratoire aigu sévère, Transformation cellulaire virale, Vecteurs génétiques.
English descriptors
- KwdEn :
- Animals, Cell Line, Cell Transformation, Viral, Chlorocebus aethiops, Coronavirus (genetics), Gene Expression, Gene Silencing, Genetic Vectors, Hemagglutinins, Viral (metabolism), Humans, Kidney (cytology), Kidney (embryology), MicroRNAs (genetics), RNA Interference, RNA, Messenger (metabolism), RNA, Small Interfering (genetics), RNA, Viral (isolation & purification), SARS Virus (genetics), Severe Acute Respiratory Syndrome (prevention & control), Vero Cells, Viral Structural Proteins (genetics), Viral Structural Proteins (metabolism).
- MESH :
- chemical , genetics : MicroRNAs, RNA, Small Interfering, Viral Structural Proteins.
- chemical , isolation & purification : RNA, Viral.
- chemical , metabolism : Hemagglutinins, Viral, RNA, Messenger, Viral Structural Proteins.
- cytology : Kidney.
- embryology : Kidney.
- genetics : Coronavirus, SARS Virus.
- prevention & control : Severe Acute Respiratory Syndrome.
- Animals, Cell Line, Cell Transformation, Viral, Chlorocebus aethiops, Gene Expression, Gene Silencing, Genetic Vectors, Humans, RNA Interference, Vero Cells.
Abstract
The severe acute respiratory syndrome (SARS) has been one of the most epidemic diseases threatening human health all over the world. Based on clinical studies, SARS‐CoV (the SARS‐associated coronavirus), a novel coronavirus, is reported as the pathogen responsible for the disease. To date, no effective and specific therapeutic method can be used to treat patients suffering from SARS‐CoV infection. RNA interference (RNAi) is a process by which the introduced small interfering RNA (siRNA) could cause the degradation of mRNA with identical sequence specificity. The RNAi methodology has been used as a tool to silence genes in cultured cells and in animals. Recently, this technique was employed in anti‐virus infections in human immunodeficiency virus and hepatitis C/B virus. In this study, RNAi technology has been applied to explore the possibility for prevention of SARS‐CoV infection. We constructed specific siRNAs targeting the S gene in SARS‐CoV. We demonstrated that the siRNAs could effectively and specifically inhibit gene expression of Spike protein in SARS‐CoV‐infected cells. Our study provided evidence that RNAi could be a tool for inhibition of SARS‐CoV.
Url:
DOI: 10.1016/S0014-5793(04)00087-0
PubMed: 14988013
PubMed Central: 7127813
Links toward previous steps (curation, corpus...)
- to stream Pmc, to step Corpus: Pour aller vers cette notice dans l'étape Curation :001009
- to stream Pmc, to step Curation: Pour aller vers cette notice dans l'étape Curation :001009
- to stream Pmc, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :001466
- to stream PubMed, to step Corpus: Pour aller vers cette notice dans l'étape Curation :002F37
- to stream PubMed, to step Curation: Pour aller vers cette notice dans l'étape Curation :002F37
- to stream PubMed, to step Checkpoint: Pour aller vers cette notice dans l'étape Curation :002A23
- to stream Ncbi, to step Merge: Pour aller vers cette notice dans l'étape Curation :000629
Links to Exploration step
PMC:7127813Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Silencing SARS‐CoV Spike protein expression in cultured cells by RNA interference</title>
<author><name sortKey="Zhang, Yuanjiang" sort="Zhang, Yuanjiang" uniqKey="Zhang Y" first="Yuanjiang" last="Zhang">Yuanjiang Zhang</name>
<affiliation><nlm:aff id="feb2s0014579304000870-aff-AFF1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Li, Tieshi" sort="Li, Tieshi" uniqKey="Li T" first="Tieshi" last="Li">Tieshi Li</name>
<affiliation><nlm:aff id="feb2s0014579304000870-aff-AFF1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Fu, Ling" sort="Fu, Ling" uniqKey="Fu L" first="Ling" last="Fu">Ling Fu</name>
<affiliation><nlm:aff id="feb2s0014579304000870-aff-AFF2"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Yu, Changming" sort="Yu, Changming" uniqKey="Yu C" first="Changming" last="Yu">Changming Yu</name>
<affiliation><nlm:aff id="feb2s0014579304000870-aff-AFF2"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Li, Yinghua" sort="Li, Yinghua" uniqKey="Li Y" first="Yinghua" last="Li">Yinghua Li</name>
<affiliation><nlm:aff id="feb2s0014579304000870-aff-AFF1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Xu, Xialian" sort="Xu, Xialian" uniqKey="Xu X" first="Xialian" last="Xu">Xialian Xu</name>
<affiliation><nlm:aff id="feb2s0014579304000870-aff-AFF1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Wang, Yinyin" sort="Wang, Yinyin" uniqKey="Wang Y" first="Yinyin" last="Wang">Yinyin Wang</name>
<affiliation><nlm:aff id="feb2s0014579304000870-aff-AFF1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Ning, Hongxiu" sort="Ning, Hongxiu" uniqKey="Ning H" first="Hongxiu" last="Ning">Hongxiu Ning</name>
<affiliation><nlm:aff id="feb2s0014579304000870-aff-AFF1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Zhang, Shuping" sort="Zhang, Shuping" uniqKey="Zhang S" first="Shuping" last="Zhang">Shuping Zhang</name>
<affiliation><nlm:aff id="feb2s0014579304000870-aff-AFF1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Chen, Wei" sort="Chen, Wei" uniqKey="Chen W" first="Wei" last="Chen">Wei Chen</name>
<affiliation><nlm:aff id="feb2s0014579304000870-aff-AFF2"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Babiuk, Lorne A" sort="Babiuk, Lorne A" uniqKey="Babiuk L" first="Lorne A" last="Babiuk">Lorne A. Babiuk</name>
<affiliation><nlm:aff id="feb2s0014579304000870-aff-AFF3"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Chang, Zhijie" sort="Chang, Zhijie" uniqKey="Chang Z" first="Zhijie" last="Chang">Zhijie Chang</name>
<affiliation><nlm:aff id="feb2s0014579304000870-aff-AFF1"></nlm:aff>
</affiliation>
</author>
</titleStmt>
<publicationStmt><idno type="wicri:source">PMC</idno>
<idno type="pmid">14988013</idno>
<idno type="pmc">7127813</idno>
<idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7127813</idno>
<idno type="RBID">PMC:7127813</idno>
<idno type="doi">10.1016/S0014-5793(04)00087-0</idno>
<date when="2004">2004</date>
<idno type="wicri:Area/Pmc/Corpus">001009</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">001009</idno>
<idno type="wicri:Area/Pmc/Curation">001009</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">001009</idno>
<idno type="wicri:Area/Pmc/Checkpoint">001466</idno>
<idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">001466</idno>
<idno type="wicri:source">PubMed</idno>
<idno type="RBID">pubmed:14988013</idno>
<idno type="wicri:Area/PubMed/Corpus">002F37</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002F37</idno>
<idno type="wicri:Area/PubMed/Curation">002F37</idno>
<idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002F37</idno>
<idno type="wicri:Area/PubMed/Checkpoint">002A23</idno>
<idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002A23</idno>
<idno type="wicri:Area/Ncbi/Merge">000629</idno>
<idno type="wicri:Area/Ncbi/Curation">000629</idno>
</publicationStmt>
<sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Silencing SARS‐CoV Spike protein expression in cultured cells by RNA interference</title>
<author><name sortKey="Zhang, Yuanjiang" sort="Zhang, Yuanjiang" uniqKey="Zhang Y" first="Yuanjiang" last="Zhang">Yuanjiang Zhang</name>
<affiliation><nlm:aff id="feb2s0014579304000870-aff-AFF1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Li, Tieshi" sort="Li, Tieshi" uniqKey="Li T" first="Tieshi" last="Li">Tieshi Li</name>
<affiliation><nlm:aff id="feb2s0014579304000870-aff-AFF1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Fu, Ling" sort="Fu, Ling" uniqKey="Fu L" first="Ling" last="Fu">Ling Fu</name>
<affiliation><nlm:aff id="feb2s0014579304000870-aff-AFF2"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Yu, Changming" sort="Yu, Changming" uniqKey="Yu C" first="Changming" last="Yu">Changming Yu</name>
<affiliation><nlm:aff id="feb2s0014579304000870-aff-AFF2"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Li, Yinghua" sort="Li, Yinghua" uniqKey="Li Y" first="Yinghua" last="Li">Yinghua Li</name>
<affiliation><nlm:aff id="feb2s0014579304000870-aff-AFF1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Xu, Xialian" sort="Xu, Xialian" uniqKey="Xu X" first="Xialian" last="Xu">Xialian Xu</name>
<affiliation><nlm:aff id="feb2s0014579304000870-aff-AFF1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Wang, Yinyin" sort="Wang, Yinyin" uniqKey="Wang Y" first="Yinyin" last="Wang">Yinyin Wang</name>
<affiliation><nlm:aff id="feb2s0014579304000870-aff-AFF1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Ning, Hongxiu" sort="Ning, Hongxiu" uniqKey="Ning H" first="Hongxiu" last="Ning">Hongxiu Ning</name>
<affiliation><nlm:aff id="feb2s0014579304000870-aff-AFF1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Zhang, Shuping" sort="Zhang, Shuping" uniqKey="Zhang S" first="Shuping" last="Zhang">Shuping Zhang</name>
<affiliation><nlm:aff id="feb2s0014579304000870-aff-AFF1"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Chen, Wei" sort="Chen, Wei" uniqKey="Chen W" first="Wei" last="Chen">Wei Chen</name>
<affiliation><nlm:aff id="feb2s0014579304000870-aff-AFF2"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Babiuk, Lorne A" sort="Babiuk, Lorne A" uniqKey="Babiuk L" first="Lorne A" last="Babiuk">Lorne A. Babiuk</name>
<affiliation><nlm:aff id="feb2s0014579304000870-aff-AFF3"></nlm:aff>
</affiliation>
</author>
<author><name sortKey="Chang, Zhijie" sort="Chang, Zhijie" uniqKey="Chang Z" first="Zhijie" last="Chang">Zhijie Chang</name>
<affiliation><nlm:aff id="feb2s0014579304000870-aff-AFF1"></nlm:aff>
</affiliation>
</author>
</analytic>
<series><title level="j">Febs Letters</title>
<idno type="ISSN">0014-5793</idno>
<idno type="eISSN">1873-3468</idno>
<imprint><date when="2004">2004</date>
</imprint>
</series>
</biblStruct>
</sourceDesc>
</fileDesc>
<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term>
<term>Cell Line</term>
<term>Cell Transformation, Viral</term>
<term>Chlorocebus aethiops</term>
<term>Coronavirus (genetics)</term>
<term>Gene Expression</term>
<term>Gene Silencing</term>
<term>Genetic Vectors</term>
<term>Hemagglutinins, Viral (metabolism)</term>
<term>Humans</term>
<term>Kidney (cytology)</term>
<term>Kidney (embryology)</term>
<term>MicroRNAs (genetics)</term>
<term>RNA Interference</term>
<term>RNA, Messenger (metabolism)</term>
<term>RNA, Small Interfering (genetics)</term>
<term>RNA, Viral (isolation & purification)</term>
<term>SARS Virus (genetics)</term>
<term>Severe Acute Respiratory Syndrome (prevention & control)</term>
<term>Vero Cells</term>
<term>Viral Structural Proteins (genetics)</term>
<term>Viral Structural Proteins (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>ARN messager (métabolisme)</term>
<term>ARN viral (isolement et purification)</term>
<term>Animaux</term>
<term>Cellules Vero</term>
<term>Coronavirus (génétique)</term>
<term>Expression des gènes</term>
<term>Extinction de l'expression des gènes</term>
<term>Humains</term>
<term>Hémagglutinines virales (métabolisme)</term>
<term>Interférence par ARN</term>
<term>Lignée cellulaire</term>
<term>Petit ARN interférent (génétique)</term>
<term>Protéines virales structurales (génétique)</term>
<term>Protéines virales structurales (métabolisme)</term>
<term>Rein (cytologie)</term>
<term>Rein (embryologie)</term>
<term>Syndrome respiratoire aigu sévère ()</term>
<term>Transformation cellulaire virale</term>
<term>Vecteurs génétiques</term>
<term>Virus du SRAS (génétique)</term>
<term>microARN (génétique)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>MicroRNAs</term>
<term>RNA, Small Interfering</term>
<term>Viral Structural Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="isolation & purification" xml:lang="en"><term>RNA, Viral</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Hemagglutinins, Viral</term>
<term>RNA, Messenger</term>
<term>Viral Structural Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="cytologie" xml:lang="fr"><term>Rein</term>
</keywords>
<keywords scheme="MESH" qualifier="cytology" xml:lang="en"><term>Kidney</term>
</keywords>
<keywords scheme="MESH" qualifier="embryologie" xml:lang="fr"><term>Rein</term>
</keywords>
<keywords scheme="MESH" qualifier="embryology" xml:lang="en"><term>Kidney</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Coronavirus</term>
<term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Coronavirus</term>
<term>Petit ARN interférent</term>
<term>Protéines virales structurales</term>
<term>Virus du SRAS</term>
<term>microARN</term>
</keywords>
<keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>ARN viral</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>ARN messager</term>
<term>Hémagglutinines virales</term>
<term>Protéines virales structurales</term>
</keywords>
<keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Animals</term>
<term>Cell Line</term>
<term>Cell Transformation, Viral</term>
<term>Chlorocebus aethiops</term>
<term>Gene Expression</term>
<term>Gene Silencing</term>
<term>Genetic Vectors</term>
<term>Humans</term>
<term>RNA Interference</term>
<term>Vero Cells</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Animaux</term>
<term>Cellules Vero</term>
<term>Expression des gènes</term>
<term>Extinction de l'expression des gènes</term>
<term>Humains</term>
<term>Interférence par ARN</term>
<term>Lignée cellulaire</term>
<term>Syndrome respiratoire aigu sévère</term>
<term>Transformation cellulaire virale</term>
<term>Vecteurs génétiques</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>The severe acute respiratory syndrome (SARS) has been one of the most epidemic diseases threatening human health all over the world. Based on clinical studies, SARS‐CoV (the SARS‐associated coronavirus), a novel coronavirus, is reported as the pathogen responsible for the disease. To date, no effective and specific therapeutic method can be used to treat patients suffering from SARS‐CoV infection. RNA interference (RNAi) is a process by which the introduced small interfering RNA (siRNA) could cause the degradation of mRNA with identical sequence specificity. The RNAi methodology has been used as a tool to silence genes in cultured cells and in animals. Recently, this technique was employed in anti‐virus infections in human immunodeficiency virus and hepatitis C/B virus. In this study, RNAi technology has been applied to explore the possibility for prevention of SARS‐CoV infection. We constructed specific siRNAs targeting the S gene in SARS‐CoV. We demonstrated that the siRNAs could effectively and specifically inhibit gene expression of Spike protein in SARS‐CoV‐infected cells. Our study provided evidence that RNAi could be a tool for inhibition of SARS‐CoV.</p>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
<biblStruct></biblStruct>
</listBibl>
</div1>
</back>
</TEI>
</record>
Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Ncbi/Curation
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 000629 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd -nk 000629 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien |wiki= Sante |area= SrasV1 |flux= Ncbi |étape= Curation |type= RBID |clé= PMC:7127813 |texte= Silencing SARS‐CoV Spike protein expression in cultured cells by RNA interference }}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Curation/RBID.i -Sk "pubmed:14988013" \ | HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Curation/biblio.hfd \ | NlmPubMed2Wicri -a SrasV1
This area was generated with Dilib version V0.6.33. |