Rapid Identification of Emerging Infectious Agents Using PCR and Electrospray Ionization Mass Spectrometry
Identifieur interne : 003F82 ( Ncbi/Checkpoint ); précédent : 003F81; suivant : 003F83Rapid Identification of Emerging Infectious Agents Using PCR and Electrospray Ionization Mass Spectrometry
Auteurs : Rangarajan Sampath ; Thomas A. Hall ; Christian Massire ; Feng Li ; Lawrence B. Blyn ; Mark W. Eshoo ; Steven A. Hofstadler ; David J. EckerSource :
- Annals of the New York Academy of Sciences [ 0077-8923 ] ; 2007.
Abstract
Url:
DOI: 10.1196/annals.1408.008
PubMed: NONE
PubMed Central: 7167958
Affiliations:
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<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Rapid Identification of Emerging Infectious Agents Using PCR and Electrospray Ionization Mass Spectrometry</title><author><name sortKey="Sampath, Rangarajan" sort="Sampath, Rangarajan" uniqKey="Sampath R" first="Rangarajan" last="Sampath">Rangarajan Sampath</name><affiliation><nlm:aff id="a1"></nlm:aff></affiliation></author><author><name sortKey="Hall, Thomas A" sort="Hall, Thomas A" uniqKey="Hall T" first="Thomas A." last="Hall">Thomas A. Hall</name><affiliation><nlm:aff id="a1"></nlm:aff></affiliation></author><author><name sortKey="Massire, Christian" sort="Massire, Christian" uniqKey="Massire C" first="Christian" last="Massire">Christian Massire</name><affiliation><nlm:aff id="a1"></nlm:aff></affiliation></author><author><name sortKey="Li, Feng" sort="Li, Feng" uniqKey="Li F" first="Feng" last="Li">Feng Li</name><affiliation><nlm:aff id="a1"></nlm:aff></affiliation></author><author><name sortKey="Blyn, Lawrence B" sort="Blyn, Lawrence B" uniqKey="Blyn L" first="Lawrence B." last="Blyn">Lawrence B. Blyn</name><affiliation><nlm:aff id="a1"></nlm:aff></affiliation></author><author><name sortKey="Eshoo, Mark W" sort="Eshoo, Mark W" uniqKey="Eshoo M" first="Mark W." last="Eshoo">Mark W. Eshoo</name><affiliation><nlm:aff id="a1"></nlm:aff></affiliation></author><author><name sortKey="Hofstadler, Steven A" sort="Hofstadler, Steven A" uniqKey="Hofstadler S" first="Steven A." last="Hofstadler">Steven A. Hofstadler</name><affiliation><nlm:aff id="a1"></nlm:aff></affiliation></author><author><name sortKey="Ecker, David J" sort="Ecker, David J" uniqKey="Ecker D" first="David J." last="Ecker">David J. Ecker</name><affiliation><nlm:aff id="a1"></nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmc">7167958</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7167958</idno><idno type="RBID">PMC:7167958</idno><idno type="doi">10.1196/annals.1408.008</idno><idno type="pmid">NONE</idno><date when="2007">2007</date><idno type="wicri:Area/Pmc/Corpus">000D84</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000D84</idno><idno type="wicri:Area/Pmc/Curation">000D84</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000D84</idno><idno type="wicri:Area/Pmc/Checkpoint">000F13</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Checkpoint">000F13</idno><idno type="wicri:Area/Ncbi/Merge">003F82</idno><idno type="wicri:Area/Ncbi/Curation">003F82</idno><idno type="wicri:Area/Ncbi/Checkpoint">003F82</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en" level="a" type="main">Rapid Identification of Emerging Infectious Agents Using PCR and Electrospray Ionization Mass Spectrometry</title><author><name sortKey="Sampath, Rangarajan" sort="Sampath, Rangarajan" uniqKey="Sampath R" first="Rangarajan" last="Sampath">Rangarajan Sampath</name><affiliation><nlm:aff id="a1"></nlm:aff></affiliation></author><author><name sortKey="Hall, Thomas A" sort="Hall, Thomas A" uniqKey="Hall T" first="Thomas A." last="Hall">Thomas A. Hall</name><affiliation><nlm:aff id="a1"></nlm:aff></affiliation></author><author><name sortKey="Massire, Christian" sort="Massire, Christian" uniqKey="Massire C" first="Christian" last="Massire">Christian Massire</name><affiliation><nlm:aff id="a1"></nlm:aff></affiliation></author><author><name sortKey="Li, Feng" sort="Li, Feng" uniqKey="Li F" first="Feng" last="Li">Feng Li</name><affiliation><nlm:aff id="a1"></nlm:aff></affiliation></author><author><name sortKey="Blyn, Lawrence B" sort="Blyn, Lawrence B" uniqKey="Blyn L" first="Lawrence B." last="Blyn">Lawrence B. Blyn</name><affiliation><nlm:aff id="a1"></nlm:aff></affiliation></author><author><name sortKey="Eshoo, Mark W" sort="Eshoo, Mark W" uniqKey="Eshoo M" first="Mark W." last="Eshoo">Mark W. Eshoo</name><affiliation><nlm:aff id="a1"></nlm:aff></affiliation></author><author><name sortKey="Hofstadler, Steven A" sort="Hofstadler, Steven A" uniqKey="Hofstadler S" first="Steven A." last="Hofstadler">Steven A. Hofstadler</name><affiliation><nlm:aff id="a1"></nlm:aff></affiliation></author><author><name sortKey="Ecker, David J" sort="Ecker, David J" uniqKey="Ecker D" first="David J." last="Ecker">David J. Ecker</name><affiliation><nlm:aff id="a1"></nlm:aff></affiliation></author></analytic><series><title level="j">Annals of the New York Academy of Sciences</title><idno type="ISSN">0077-8923</idno><idno type="eISSN">1749-6632</idno><imprint><date when="2007">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p><bold><sc>abstract: </sc></bold><bold>Newly emergent infectious diseases are a global public health problem. The population dense regions of Southeast Asia are the epicenter of many emerging diseases, as evidenced by the outbreak of Nipah, SARS, avian influenza (H5N1), Dengue, and enterovirus 71 in this region in the past decade. Rapid identification, epidemiologic surveillance, and mitigation of transmission are major challenges in ensuring public health safety. Here we describe a powerful new approach for infectious disease surveillance that is based on polymerase chain reaction (PCR) to amplify nucleic acid targets from large groupings of organisms, electrospray ionization mass spectrometry (ESI‐MS) for accurate mass measurements of the PCR products, and base composition signature analysis to identify organisms in a sample. This approach is capable of automated analysis of more than 1,500 PCR reactions a day. It is applicable to the surveillance of bacterial, viral, fungal, or protozoal pathogens and will facilitate rapid characterization of known and emerging pathogens.</bold></p></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct><analytic><author><name sortKey="Knobler, S E" uniqKey="Knobler S">S.E. Knobler</name></author></analytic></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct></listBibl></div1></back></TEI><affiliations><list></list><tree><noCountry><name sortKey="Blyn, Lawrence B" sort="Blyn, Lawrence B" uniqKey="Blyn L" first="Lawrence B." last="Blyn">Lawrence B. Blyn</name><name sortKey="Ecker, David J" sort="Ecker, David J" uniqKey="Ecker D" first="David J." last="Ecker">David J. Ecker</name><name sortKey="Eshoo, Mark W" sort="Eshoo, Mark W" uniqKey="Eshoo M" first="Mark W." last="Eshoo">Mark W. Eshoo</name><name sortKey="Hall, Thomas A" sort="Hall, Thomas A" uniqKey="Hall T" first="Thomas A." last="Hall">Thomas A. Hall</name><name sortKey="Hofstadler, Steven A" sort="Hofstadler, Steven A" uniqKey="Hofstadler S" first="Steven A." last="Hofstadler">Steven A. Hofstadler</name><name sortKey="Li, Feng" sort="Li, Feng" uniqKey="Li F" first="Feng" last="Li">Feng Li</name><name sortKey="Massire, Christian" sort="Massire, Christian" uniqKey="Massire C" first="Christian" last="Massire">Christian Massire</name><name sortKey="Sampath, Rangarajan" sort="Sampath, Rangarajan" uniqKey="Sampath R" first="Rangarajan" last="Sampath">Rangarajan Sampath</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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