Detection of feline coronavirus using microcantilever sensors.
Identifieur interne : 003986 ( Ncbi/Checkpoint ); précédent : 003985; suivant : 003987Detection of feline coronavirus using microcantilever sensors.
Auteurs : Sreepriya Velanki [États-Unis] ; Hai-Feng Ji [États-Unis]Source :
- Measurement science & technology [ 0957-0233 ] ; 2006.
Abstract
This work demonstrated the feasibility of detecting severe acute respiratory syndrome associated coronavirus (SARS-CoV) using microcantilever technology by showing that the feline coronavirus (FIP) type I virus can be detected by a microcantilever modified by feline coronavirus (FIP) type I anti-viral antiserum. A microcantilever modified by FIP type I anti-viral antiserum was developed for the detection of FIP type I virus. When the FIP type I virus positive sample is injected into the fluid cell where the microcantilever is held, the microcantilever bends upon the recognition of the FIP type I virus by the antiserum on the surface of the microcantilever. A negative control sample that does not contain FIP type I virus did not cause any bending of the microcantilever. The detection limit of the sensor was 0.1 µg ml-1 when the assay time was <1 h.
DOI: 10.1088/0957-0233/17/11/015
PubMed: 32288326
Affiliations:
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<front><div type="abstract" xml:lang="en">This work demonstrated the feasibility of detecting severe acute respiratory syndrome associated coronavirus (SARS-CoV) using microcantilever technology by showing that the feline coronavirus (FIP) type I virus can be detected by a microcantilever modified by feline coronavirus (FIP) type I anti-viral antiserum. A microcantilever modified by FIP type I anti-viral antiserum was developed for the detection of FIP type I virus. When the FIP type I virus positive sample is injected into the fluid cell where the microcantilever is held, the microcantilever bends upon the recognition of the FIP type I virus by the antiserum on the surface of the microcantilever. A negative control sample that does not contain FIP type I virus did not cause any bending of the microcantilever. The detection limit of the sensor was 0.1 µg ml<sup>-1</sup>
when the assay time was <1 h.</div>
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