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Detection of feline coronavirus using microcantilever sensors.

Identifieur interne : 003986 ( Ncbi/Checkpoint ); précédent : 003985; suivant : 003987

Detection of feline coronavirus using microcantilever sensors.

Auteurs : Sreepriya Velanki [États-Unis] ; Hai-Feng Ji [États-Unis]

Source :

RBID : pubmed:32288326

Abstract

This work demonstrated the feasibility of detecting severe acute respiratory syndrome associated coronavirus (SARS-CoV) using microcantilever technology by showing that the feline coronavirus (FIP) type I virus can be detected by a microcantilever modified by feline coronavirus (FIP) type I anti-viral antiserum. A microcantilever modified by FIP type I anti-viral antiserum was developed for the detection of FIP type I virus. When the FIP type I virus positive sample is injected into the fluid cell where the microcantilever is held, the microcantilever bends upon the recognition of the FIP type I virus by the antiserum on the surface of the microcantilever. A negative control sample that does not contain FIP type I virus did not cause any bending of the microcantilever. The detection limit of the sensor was 0.1 µg ml-1 when the assay time was <1 h.

DOI: 10.1088/0957-0233/17/11/015
PubMed: 32288326


Affiliations:


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pubmed:32288326

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