Investigation of the functional roles of host cell proteins involved in coronavirus infection using highly specific and scalable RNA interference (RNAi) approach.
Identifieur interne : 002A37 ( Ncbi/Checkpoint ); précédent : 002A36; suivant : 002A38Investigation of the functional roles of host cell proteins involved in coronavirus infection using highly specific and scalable RNA interference (RNAi) approach.
Auteurs : Jean Kaoru Millet [États-Unis] ; Béatrice NalSource :
- Methods in molecular biology (Clifton, N.J.) [ 1940-6029 ] ; 2015.
Descripteurs français
- KwdFr :
- MESH :
English descriptors
- KwdEn :
- MESH :
- chemical , physiology : Cytoskeletal Proteins.
- physiology : SARS Virus.
- Gene Knockdown Techniques, HeLa Cells, Host-Pathogen Interactions, Humans, RNA Interference, Virus Internalization.
Abstract
Since its identification in the 1990s, the RNA interference (RNAi) pathway has proven extremely useful in elucidating the function of proteins in the context of cells and even whole organisms. In particular, this sequence-specific and powerful loss-of-function approach has greatly simplified the study of the role of host cell factors implicated in the life cycle of viruses. Here, we detail the RNAi method we have developed and used to specifically knock down the expression of ezrin, an actin binding protein that was identified by yeast two-hybrid screening to interact with the Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) spike (S) protein. This method was used to study the role of ezrin, specifically during the entry stage of SARS-CoV infection.
DOI: 10.1007/978-1-4939-2438-7_19
PubMed: 25720484
Affiliations:
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pubmed:25720484Le document en format XML
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In particular, this sequence-specific and powerful loss-of-function approach has greatly simplified the study of the role of host cell factors implicated in the life cycle of viruses. Here, we detail the RNAi method we have developed and used to specifically knock down the expression of ezrin, an actin binding protein that was identified by yeast two-hybrid screening to interact with the Severe Acute Respiratory Syndrome Coronavirus (SARS-CoV) spike (S) protein. This method was used to study the role of ezrin, specifically during the entry stage of SARS-CoV infection. </div></front></TEI><affiliations><list><country><li>États-Unis</li></country><region><li>État de New York</li></region><settlement><li>Ithaca (New York)</li></settlement><orgName><li>Université Cornell</li></orgName></list><tree><noCountry><name sortKey="Nal, Beatrice" sort="Nal, Beatrice" uniqKey="Nal B" first="Béatrice" last="Nal">Béatrice Nal</name></noCountry><country name="États-Unis"><region name="État de New York"><name sortKey="Millet, Jean Kaoru" sort="Millet, Jean Kaoru" uniqKey="Millet J" first="Jean Kaoru" last="Millet">Jean Kaoru Millet</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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