Cell culture and electron microscopy for identifying viruses in diseases of unknown cause.
Identifieur interne : 002684 ( Ncbi/Checkpoint ); précédent : 002683; suivant : 002685Cell culture and electron microscopy for identifying viruses in diseases of unknown cause.
Auteurs : Cynthia S. Goldsmith [États-Unis] ; Thomas G. Ksiazek ; Pierre E. Rollin ; James A. Comer ; William L. Nicholson ; Teresa C T. Peret ; Dean D. Erdman ; William J. Bellini ; Brian H. Harcourt ; Paul A. Rota ; Julu Bhatnagar ; Michael D. Bowen ; Bobbie R. Erickson ; Laura K. Mcmullan ; Stuart T. Nichol ; Wun-Ju Shieh ; Christopher D. Paddock ; Sherif R. ZakiSource :
- Emerging infectious diseases [ 1080-6059 ] ; 2013.
Descripteurs français
- KwdFr :
- Arenaviridae (isolement et purification), Arenaviridae (ultrastructure), Bunyaviridae (isolement et purification), Bunyaviridae (ultrastructure), Coronaviridae (isolement et purification), Coronaviridae (ultrastructure), Flaviviridae (isolement et purification), Flaviviridae (ultrastructure), Humains, Maladies virales (diagnostic), Maladies virales (virologie), Maladies virales (épidémiologie), Microscopie électronique, Paramyxoviridae (isolement et purification), Paramyxoviridae (ultrastructure), Techniques de culture cellulaire, Virus (isolement et purification), Virus (ultrastructure), États-Unis d'Amérique (épidémiologie).
- MESH :
- diagnostic : Maladies virales.
- isolement et purification : Arenaviridae, Bunyaviridae, Coronaviridae, Flaviviridae, Paramyxoviridae, Virus.
- virologie : Maladies virales.
- épidémiologie : Arenaviridae, Bunyaviridae, Coronaviridae, Flaviviridae, Maladies virales, Paramyxoviridae, Virus, États-Unis d'Amérique.
- Humains, Microscopie électronique, Techniques de culture cellulaire.
- Wicri :
- geographic : États-Unis.
English descriptors
- KwdEn :
- Arenaviridae (isolation & purification), Arenaviridae (ultrastructure), Bunyaviridae (isolation & purification), Bunyaviridae (ultrastructure), Cell Culture Techniques, Coronaviridae (isolation & purification), Coronaviridae (ultrastructure), Flaviviridae (isolation & purification), Flaviviridae (ultrastructure), Humans, Microscopy, Electron, Paramyxoviridae (isolation & purification), Paramyxoviridae (ultrastructure), United States (epidemiology), Virus Diseases (diagnosis), Virus Diseases (epidemiology), Virus Diseases (virology), Viruses (isolation & purification), Viruses (ultrastructure).
- MESH :
- geographic , epidemiology : United States.
- diagnosis : Virus Diseases.
- epidemiology : Virus Diseases.
- isolation & purification : Arenaviridae, Bunyaviridae, Coronaviridae, Flaviviridae, Paramyxoviridae, Viruses.
- ultrastructure : Arenaviridae, Bunyaviridae, Coronaviridae, Flaviviridae, Paramyxoviridae, Viruses.
- virology : Virus Diseases.
- Cell Culture Techniques, Humans, Microscopy, Electron.
Abstract
During outbreaks of infectious diseases or in cases of severely ill patients, it is imperative to identify the causative agent. This report describes several events in which virus isolation and identification by electron microscopy were critical to initial recognition of the etiologic agent, which was further analyzed by additional laboratory diagnostic assays. Examples include severe acute respiratory syndrome coronavirus, and Nipah, lymphocytic choriomeningitis, West Nile, Cache Valley, and Heartland viruses. These cases illustrate the importance of the techniques of cell culture and electron microscopy in pathogen identification and recognition of emerging diseases.
DOI: 10.3201/eid1906.130173
PubMed: 23731788
Affiliations:
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- to stream Ncbi, to step Merge: 002684
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Links to Exploration step
pubmed:23731788Le document en format XML
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<term>Bunyaviridae (isolation & purification)</term>
<term>Bunyaviridae (ultrastructure)</term>
<term>Cell Culture Techniques</term>
<term>Coronaviridae (isolation & purification)</term>
<term>Coronaviridae (ultrastructure)</term>
<term>Flaviviridae (isolation & purification)</term>
<term>Flaviviridae (ultrastructure)</term>
<term>Humans</term>
<term>Microscopy, Electron</term>
<term>Paramyxoviridae (isolation & purification)</term>
<term>Paramyxoviridae (ultrastructure)</term>
<term>United States (epidemiology)</term>
<term>Virus Diseases (diagnosis)</term>
<term>Virus Diseases (epidemiology)</term>
<term>Virus Diseases (virology)</term>
<term>Viruses (isolation & purification)</term>
<term>Viruses (ultrastructure)</term>
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<term>Arenaviridae (ultrastructure)</term>
<term>Bunyaviridae (isolement et purification)</term>
<term>Bunyaviridae (ultrastructure)</term>
<term>Coronaviridae (isolement et purification)</term>
<term>Coronaviridae (ultrastructure)</term>
<term>Flaviviridae (isolement et purification)</term>
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<front><div type="abstract" xml:lang="en">During outbreaks of infectious diseases or in cases of severely ill patients, it is imperative to identify the causative agent. This report describes several events in which virus isolation and identification by electron microscopy were critical to initial recognition of the etiologic agent, which was further analyzed by additional laboratory diagnostic assays. Examples include severe acute respiratory syndrome coronavirus, and Nipah, lymphocytic choriomeningitis, West Nile, Cache Valley, and Heartland viruses. These cases illustrate the importance of the techniques of cell culture and electron microscopy in pathogen identification and recognition of emerging diseases.</div>
</front>
</TEI>
<affiliations><list><country><li>États-Unis</li>
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<region><li>Géorgie (États-Unis)</li>
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<tree><noCountry><name sortKey="Bellini, William J" sort="Bellini, William J" uniqKey="Bellini W" first="William J" last="Bellini">William J. Bellini</name>
<name sortKey="Bhatnagar, Julu" sort="Bhatnagar, Julu" uniqKey="Bhatnagar J" first="Julu" last="Bhatnagar">Julu Bhatnagar</name>
<name sortKey="Bowen, Michael D" sort="Bowen, Michael D" uniqKey="Bowen M" first="Michael D" last="Bowen">Michael D. Bowen</name>
<name sortKey="Comer, James A" sort="Comer, James A" uniqKey="Comer J" first="James A" last="Comer">James A. Comer</name>
<name sortKey="Erdman, Dean D" sort="Erdman, Dean D" uniqKey="Erdman D" first="Dean D" last="Erdman">Dean D. Erdman</name>
<name sortKey="Erickson, Bobbie R" sort="Erickson, Bobbie R" uniqKey="Erickson B" first="Bobbie R" last="Erickson">Bobbie R. Erickson</name>
<name sortKey="Harcourt, Brian H" sort="Harcourt, Brian H" uniqKey="Harcourt B" first="Brian H" last="Harcourt">Brian H. Harcourt</name>
<name sortKey="Ksiazek, Thomas G" sort="Ksiazek, Thomas G" uniqKey="Ksiazek T" first="Thomas G" last="Ksiazek">Thomas G. Ksiazek</name>
<name sortKey="Mcmullan, Laura K" sort="Mcmullan, Laura K" uniqKey="Mcmullan L" first="Laura K" last="Mcmullan">Laura K. Mcmullan</name>
<name sortKey="Nichol, Stuart T" sort="Nichol, Stuart T" uniqKey="Nichol S" first="Stuart T" last="Nichol">Stuart T. Nichol</name>
<name sortKey="Nicholson, William L" sort="Nicholson, William L" uniqKey="Nicholson W" first="William L" last="Nicholson">William L. Nicholson</name>
<name sortKey="Paddock, Christopher D" sort="Paddock, Christopher D" uniqKey="Paddock C" first="Christopher D" last="Paddock">Christopher D. Paddock</name>
<name sortKey="Peret, Teresa C T" sort="Peret, Teresa C T" uniqKey="Peret T" first="Teresa C T" last="Peret">Teresa C T. Peret</name>
<name sortKey="Rollin, Pierre E" sort="Rollin, Pierre E" uniqKey="Rollin P" first="Pierre E" last="Rollin">Pierre E. Rollin</name>
<name sortKey="Rota, Paul A" sort="Rota, Paul A" uniqKey="Rota P" first="Paul A" last="Rota">Paul A. Rota</name>
<name sortKey="Shieh, Wun Ju" sort="Shieh, Wun Ju" uniqKey="Shieh W" first="Wun-Ju" last="Shieh">Wun-Ju Shieh</name>
<name sortKey="Zaki, Sherif R" sort="Zaki, Sherif R" uniqKey="Zaki S" first="Sherif R" last="Zaki">Sherif R. Zaki</name>
</noCountry>
<country name="États-Unis"><region name="Géorgie (États-Unis)"><name sortKey="Goldsmith, Cynthia S" sort="Goldsmith, Cynthia S" uniqKey="Goldsmith C" first="Cynthia S" last="Goldsmith">Cynthia S. Goldsmith</name>
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