SARS-CoV regulates immune function-related gene expression in human monocytic cells.
Identifieur interne : 002547 ( Ncbi/Checkpoint ); précédent : 002546; suivant : 002548SARS-CoV regulates immune function-related gene expression in human monocytic cells.
Auteurs : Wanchung Hu [République populaire de Chine] ; Yu-Ting Yen ; Sher Singh ; Chuan-Liang Kao ; Betty A. Wu-HsiehSource :
- Viral immunology [ 1557-8976 ] ; 2012.
Descripteurs français
- KwdFr :
- ARN messager (génétique), ARN messager (métabolisme), Analyse de profil d'expression de gènes, Coronavirus humain 229E (immunologie), Coronavirus humain 229E (physiologie), Humains, Interleukine-8 (génétique), Interleukine-8 (métabolisme), Monocytes (immunologie), Monocytes (métabolisme), Monocytes (virologie), Proteasome endopeptidase complex (génétique), Proteasome endopeptidase complex (métabolisme), Régulation de l'expression des gènes (immunologie), Réseaux de régulation génique, Syndrome respiratoire aigu sévère, Séquençage par oligonucléotides en batterie, Virus du SRAS (immunologie), Virus du SRAS (physiologie).
- MESH :
- génétique : ARN messager, Interleukine-8, Proteasome endopeptidase complex.
- immunologie : Coronavirus humain 229E, Monocytes, Régulation de l'expression des gènes, Virus du SRAS.
- métabolisme : ARN messager, Interleukine-8, Monocytes, Proteasome endopeptidase complex.
- physiologie : Coronavirus humain 229E, Virus du SRAS.
- virologie : Monocytes.
- Analyse de profil d'expression de gènes, Humains, Réseaux de régulation génique, Syndrome respiratoire aigu sévère, Séquençage par oligonucléotides en batterie.
English descriptors
- KwdEn :
- Coronavirus 229E, Human (immunology), Coronavirus 229E, Human (physiology), Gene Expression Profiling, Gene Expression Regulation (immunology), Gene Regulatory Networks, Humans, Interleukin-8 (genetics), Interleukin-8 (metabolism), Monocytes (immunology), Monocytes (metabolism), Monocytes (virology), Oligonucleotide Array Sequence Analysis, Proteasome Endopeptidase Complex (genetics), Proteasome Endopeptidase Complex (metabolism), RNA, Messenger (genetics), RNA, Messenger (metabolism), SARS Virus (immunology), SARS Virus (physiology), Severe Acute Respiratory Syndrome.
- MESH :
- chemical , genetics : Interleukin-8, Proteasome Endopeptidase Complex, RNA, Messenger.
- immunology : Coronavirus 229E, Human, Gene Expression Regulation, Monocytes, SARS Virus.
- chemical , metabolism : Interleukin-8, Monocytes, Proteasome Endopeptidase Complex, RNA, Messenger.
- physiology : Coronavirus 229E, Human, SARS Virus.
- virology : Monocytes.
- Gene Expression Profiling, Gene Regulatory Networks, Humans, Oligonucleotide Array Sequence Analysis, Severe Acute Respiratory Syndrome.
Abstract
Severe acute respiratory syndrome (SARS) is characterized by acute respiratory distress syndrome (ARDS) and pulmonary fibrosis, and monocytes/macrophages are the key players in the pathogenesis of SARS. In this study, we compared the transcriptional profiles of SARS coronavirus (SARS-CoV)-infected monocytic cells against that infected by coronavirus 229E (CoV-229E). Total RNA was extracted from infected DC-SIGN-transfected monocytes (THP-1-DC-SIGN) at 6 and 24 h after infection, and the gene expression was profiled in oligonucleotide-based microarrays. Analysis of immune-related gene expression profiles showed that at 24 h after SARS-CoV infection: (1) IFN-α/β-inducible and cathepsin/proteasome genes were downregulated; (2) hypoxia/hyperoxia-related genes were upregulated; and (3) TLR/TLR-signaling, cytokine/cytokine receptor-related, chemokine/chemokine receptor-related, lysosome-related, MHC/chaperon-related, and fibrosis-related genes were differentially regulated. These results elucidate that SARS-CoV infection regulates immune-related genes in monocytes/macrophages, which may be important to the pathogenesis of SARS.
DOI: 10.1089/vim.2011.0099
PubMed: 22876772
Affiliations:
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pubmed:22876772Le document en format XML
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In this study, we compared the transcriptional profiles of SARS coronavirus (SARS-CoV)-infected monocytic cells against that infected by coronavirus 229E (CoV-229E). Total RNA was extracted from infected DC-SIGN-transfected monocytes (THP-1-DC-SIGN) at 6 and 24 h after infection, and the gene expression was profiled in oligonucleotide-based microarrays. Analysis of immune-related gene expression profiles showed that at 24 h after SARS-CoV infection: (1) IFN-α/β-inducible and cathepsin/proteasome genes were downregulated; (2) hypoxia/hyperoxia-related genes were upregulated; and (3) TLR/TLR-signaling, cytokine/cytokine receptor-related, chemokine/chemokine receptor-related, lysosome-related, MHC/chaperon-related, and fibrosis-related genes were differentially regulated. These results elucidate that SARS-CoV infection regulates immune-related genes in monocytes/macrophages, which may be important to the pathogenesis of SARS.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Kao, Chuan Liang" sort="Kao, Chuan Liang" uniqKey="Kao C" first="Chuan-Liang" last="Kao">Chuan-Liang Kao</name><name sortKey="Singh, Sher" sort="Singh, Sher" uniqKey="Singh S" first="Sher" last="Singh">Sher Singh</name><name sortKey="Wu Hsieh, Betty A" sort="Wu Hsieh, Betty A" uniqKey="Wu Hsieh B" first="Betty A" last="Wu-Hsieh">Betty A. Wu-Hsieh</name><name sortKey="Yen, Yu Ting" sort="Yen, Yu Ting" uniqKey="Yen Y" first="Yu-Ting" last="Yen">Yu-Ting Yen</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Hu, Wanchung" sort="Hu, Wanchung" uniqKey="Hu W" first="Wanchung" last="Hu">Wanchung Hu</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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