Mutagenesis of the transmembrane domain of the SARS coronavirus spike glycoprotein: refinement of the requirements for SARS coronavirus cell entry.
Identifieur interne : 002054 ( Ncbi/Checkpoint ); précédent : 002053; suivant : 002055Mutagenesis of the transmembrane domain of the SARS coronavirus spike glycoprotein: refinement of the requirements for SARS coronavirus cell entry.
Auteurs : Jeroen Corver [Pays-Bas] ; Rene Broer ; Puck Van Kasteren ; Willy SpaanSource :
- Virology journal [ 1743-422X ] ; 2009.
Descripteurs français
- KwdFr :
- Données de séquences moléculaires, Fusion cellulaire, Fusion membranaire, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (), Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (métabolisme), Humains, Interactions hydrophobes et hydrophiles, Lignée cellulaire, Mutagenèse, Protéines de l'enveloppe virale (), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (métabolisme), Séquence d'acides aminés, Virus du SRAS (génétique), Virus du SRAS (pathogénicité).
- MESH :
- génétique : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Virus du SRAS.
- métabolisme : Glycoprotéines membranaires, Protéines de l'enveloppe virale.
- pathogénicité : Virus du SRAS.
- Données de séquences moléculaires, Fusion cellulaire, Fusion membranaire, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires, Humains, Interactions hydrophobes et hydrophiles, Lignée cellulaire, Mutagenèse, Protéines de l'enveloppe virale, Séquence d'acides aminés.
English descriptors
- KwdEn :
- Amino Acid Sequence, Cell Fusion, Cell Line, Humans, Hydrophobic and Hydrophilic Interactions, Membrane Fusion, Membrane Glycoproteins (chemistry), Membrane Glycoproteins (genetics), Membrane Glycoproteins (metabolism), Molecular Sequence Data, Mutagenesis, SARS Virus (genetics), SARS Virus (pathogenicity), Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (chemistry), Viral Envelope Proteins (genetics), Viral Envelope Proteins (metabolism).
- MESH :
- chemical , chemistry : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , genetics : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , metabolism : Membrane Glycoproteins, Viral Envelope Proteins.
- genetics : SARS Virus.
- pathogenicity : SARS Virus.
- Amino Acid Sequence, Cell Fusion, Cell Line, Humans, Hydrophobic and Hydrophilic Interactions, Membrane Fusion, Molecular Sequence Data, Mutagenesis, Spike Glycoprotein, Coronavirus.
Abstract
The spike protein (S) of SARS Coronavirus (SARS-CoV) mediates entry of the virus into target cells, including receptor binding and membrane fusion. Close to or in the viral membrane, the S protein contains three distinct motifs: a juxtamembrane aromatic part, a central highly hydrophobic stretch and a cysteine rich motif. Here, we investigate the role of aromatic and hydrophobic parts of S in the entry of SARS CoV and in cell-cell fusion. This was investigated using the previously described SARS pseudotyped particles system (SARSpp) and by fluorescence-based cell-cell fusion assays.
DOI: 10.1186/1743-422X-6-230
PubMed: 20034394
Affiliations:
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pubmed:20034394Le document en format XML
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aminés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The spike protein (S) of SARS Coronavirus (SARS-CoV) mediates entry of the virus into target cells, including receptor binding and membrane fusion. Close to or in the viral membrane, the S protein contains three distinct motifs: a juxtamembrane aromatic part, a central highly hydrophobic stretch and a cysteine rich motif. Here, we investigate the role of aromatic and hydrophobic parts of S in the entry of SARS CoV and in cell-cell fusion. This was investigated using the previously described SARS pseudotyped particles system (SARSpp) and by fluorescence-based cell-cell fusion assays.</div></front></TEI><affiliations><list><country><li>Pays-Bas</li></country></list><tree><noCountry><name sortKey="Broer, Rene" sort="Broer, Rene" uniqKey="Broer R" first="Rene" last="Broer">Rene Broer</name><name sortKey="Spaan, Willy" sort="Spaan, Willy" uniqKey="Spaan W" first="Willy" last="Spaan">Willy Spaan</name><name sortKey="Van Kasteren, Puck" sort="Van Kasteren, Puck" uniqKey="Van Kasteren P" first="Puck" last="Van Kasteren">Puck Van Kasteren</name></noCountry><country name="Pays-Bas"><noRegion><name sortKey="Corver, Jeroen" sort="Corver, Jeroen" uniqKey="Corver J" first="Jeroen" last="Corver">Jeroen Corver</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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