Pathways of Cross-Species Transmission of Synthetically Reconstructed Zoonotic Severe Acute Respiratory Syndrome Coronavirus▿
Identifieur interne : 001C85 ( Ncbi/Checkpoint ); précédent : 001C84; suivant : 001C86Pathways of Cross-Species Transmission of Synthetically Reconstructed Zoonotic Severe Acute Respiratory Syndrome Coronavirus▿
Auteurs : Timothy Sheahan ; Barry Rockx ; Eric Donaldson ; Davide Corti ; Ralph BaricSource :
- Journal of Virology [ 0022-538X ] ; 2008.
Descripteurs français
- KwdFr :
- Analyse de séquence d'ADN, Animaux, Anticorps monoclonaux (immunologie), Cellules Vero, Chine (épidémiologie), Chiroptera (virologie), Cinétique, Données de séquences moléculaires, Effet cytopathogène viral, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires (), Glycoprotéines membranaires (génétique), Glycoprotéines membranaires (métabolisme), Gènes viraux, Humains, Liaison aux protéines, Lignée cellulaire, Matrices (génétique), Modèles moléculaires, Mutation ponctuelle, Plasmides, Protéines de l'enveloppe virale (), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (métabolisme), Protéines à fluorescence verte (métabolisme), Récepteurs viraux (), Récepteurs viraux (génétique), Récepteurs viraux (métabolisme), Réservoirs d'agents pathogènes, Structure secondaire des protéines, Structure tertiaire des protéines, Syndrome respiratoire aigu sévère (anatomopathologie), Syndrome respiratoire aigu sévère (épidémiologie), Syndrome respiratoire aigu sévère (étiologie), Séquence d'acides aminés, Tests de neutralisation, Transfection, Virus du SRAS (croissance et développement), Virus du SRAS (isolement et purification), Virus du SRAS (pathogénicité), Viverridae (virologie), Zoonoses (transmission), Zoonoses (virologie), Zoonoses (épidémiologie).
- MESH :
- anatomopathologie : Syndrome respiratoire aigu sévère.
- croissance et développement : Virus du SRAS.
- génétique : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Récepteurs viraux.
- immunologie : Anticorps monoclonaux.
- isolement et purification : Virus du SRAS.
- métabolisme : Glycoprotéines membranaires, Protéines de l'enveloppe virale, Protéines à fluorescence verte, Récepteurs viraux.
- pathogénicité : Virus du SRAS.
- virologie : Chiroptera, Viverridae, Zoonoses.
- épidémiologie : Chine, Syndrome respiratoire aigu sévère, Zoonoses.
- étiologie : Syndrome respiratoire aigu sévère.
- Analyse de séquence d'ADN, Animaux, Cellules Vero, Cinétique, Données de séquences moléculaires, Effet cytopathogène viral, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires, Gènes viraux, Humains, Liaison aux protéines, Lignée cellulaire, Matrices (génétique), Modèles moléculaires, Mutation ponctuelle, Plasmides, Protéines de l'enveloppe virale, Récepteurs viraux, Réservoirs d'agents pathogènes, Structure secondaire des protéines, Structure tertiaire des protéines, Séquence d'acides aminés, Tests de neutralisation, Transfection.
- Wicri :
- geographic : République populaire de Chine.
English descriptors
- KwdEn :
- Amino Acid Sequence, Animals, Antibodies, Monoclonal (immunology), Cell Line, China (epidemiology), Chiroptera (virology), Chlorocebus aethiops, Cytopathogenic Effect, Viral, Disease Reservoirs, Genes, Viral, Green Fluorescent Proteins (metabolism), Humans, Kinetics, Membrane Glycoproteins (chemistry), Membrane Glycoproteins (genetics), Membrane Glycoproteins (metabolism), Models, Molecular, Molecular Sequence Data, Neutralization Tests, Plasmids, Point Mutation, Protein Binding, Protein Structure, Secondary, Protein Structure, Tertiary, Receptors, Virus (chemistry), Receptors, Virus (genetics), Receptors, Virus (metabolism), SARS Virus (growth & development), SARS Virus (isolation & purification), SARS Virus (pathogenicity), Sequence Analysis, DNA, Severe Acute Respiratory Syndrome (epidemiology), Severe Acute Respiratory Syndrome (etiology), Severe Acute Respiratory Syndrome (pathology), Spike Glycoprotein, Coronavirus, Templates, Genetic, Transfection, Vero Cells, Viral Envelope Proteins (chemistry), Viral Envelope Proteins (genetics), Viral Envelope Proteins (metabolism), Viverridae (virology), Zoonoses (epidemiology), Zoonoses (transmission), Zoonoses (virology).
- MESH :
- chemical , chemistry : Membrane Glycoproteins, Receptors, Virus, Viral Envelope Proteins.
- chemical , genetics : Membrane Glycoproteins, Receptors, Virus, Viral Envelope Proteins.
- chemical , immunology : Antibodies, Monoclonal.
- geographic , epidemiology : China.
- chemical , metabolism : Green Fluorescent Proteins, Membrane Glycoproteins, Receptors, Virus, Viral Envelope Proteins.
- epidemiology : Severe Acute Respiratory Syndrome, Zoonoses.
- etiology : Severe Acute Respiratory Syndrome.
- growth & development : SARS Virus.
- isolation & purification : SARS Virus.
- pathogenicity : SARS Virus.
- pathology : Severe Acute Respiratory Syndrome.
- transmission : Zoonoses.
- virology : Chiroptera, Viverridae, Zoonoses.
- Amino Acid Sequence, Animals, Cell Line, Chlorocebus aethiops, Cytopathogenic Effect, Viral, Disease Reservoirs, Genes, Viral, Humans, Kinetics, Models, Molecular, Molecular Sequence Data, Neutralization Tests, Plasmids, Point Mutation, Protein Binding, Protein Structure, Secondary, Protein Structure, Tertiary, Sequence Analysis, DNA, Spike Glycoprotein, Coronavirus, Templates, Genetic, Transfection, Vero Cells.
Abstract
Zoonotic severe acute respiratory syndrome coronavirus (SARS-CoV) likely evolved to infect humans by a series of transmission events between humans and animals in markets in China. Virus sequence data suggest that the palm civet served as an amplification host in which civet and human interaction fostered the evolution of the epidemic SARS Urbani strain. The prototypic civet strain of SARS-CoV, SZ16, was isolated from a palm civet but has not been successfully cultured in vitro. To propagate a chimeric recombinant SARS-CoV bearing an SZ16 spike (S) glycoprotein (icSZ16-S), we constructed cell lines expressing the civet ortholog (DBT-cACE2) of the SARS-CoV receptor (hACE2). Zoonotic SARS-CoV was completely dependent on ACE2 for entry. Urbani grew with similar kinetics in both the DBT-cACE2 and the DBT-hACE2 cells, while icSZ16-S only grew in DBT-cACE2 cells. The SZ16-S mutant viruses adapted to human airway epithelial cells and displayed enhanced affinity for hACE2 but exhibited severe growth defects in the DBT-cACE2 cells, suggesting that the evolutionary pathway that promoted efficient hACE2 interactions simultaneously abolished efficient cACE2 interactions. Structural modeling predicted two distinct biochemical interaction networks by which zoonotic receptor binding domain architecture can productively engage hACE2, but only the Urbani mutational repertoire promoted efficient usage of both hACE2 and cACE2 binding interfaces. Since dual species tropism was preserved in Urbani, it is likely that the virus evolved a high affinity for cACE2/hACE2 receptors through adaptation via repeated passages between human and civet hosts. Furthermore, zoonotic SARS-CoV was variably neutralized by antibodies that were effective against the epidemic strain, highlighting their utility for evaluating passive immunization efficacy.
Url:
DOI: 10.1128/JVI.00818-08
PubMed: 18579604
PubMed Central: 2519660
Affiliations:
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sortKey="Sheahan, Timothy" sort="Sheahan, Timothy" uniqKey="Sheahan T" first="Timothy" last="Sheahan">Timothy Sheahan</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Rockx, Barry" sort="Rockx, Barry" uniqKey="Rockx B" first="Barry" last="Rockx">Barry Rockx</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Donaldson, Eric" sort="Donaldson, Eric" uniqKey="Donaldson E" first="Eric" last="Donaldson">Eric Donaldson</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Corti, Davide" sort="Corti, Davide" uniqKey="Corti D" first="Davide" last="Corti">Davide Corti</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author><author><name sortKey="Baric, Ralph" sort="Baric, Ralph" uniqKey="Baric R" first="Ralph" last="Baric">Ralph Baric</name><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation><affiliation><nlm:aff id="aff1"></nlm:aff></affiliation></author></analytic><series><title level="j">Journal of Virology</title><idno type="ISSN">0022-538X</idno><idno type="eISSN">1098-5514</idno><imprint><date when="2008">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Antibodies, Monoclonal (immunology)</term><term>Cell Line</term><term>China (epidemiology)</term><term>Chiroptera (virology)</term><term>Chlorocebus aethiops</term><term>Cytopathogenic Effect, Viral</term><term>Disease Reservoirs</term><term>Genes, Viral</term><term>Green Fluorescent Proteins (metabolism)</term><term>Humans</term><term>Kinetics</term><term>Membrane Glycoproteins (chemistry)</term><term>Membrane Glycoproteins (genetics)</term><term>Membrane Glycoproteins (metabolism)</term><term>Models, Molecular</term><term>Molecular Sequence Data</term><term>Neutralization Tests</term><term>Plasmids</term><term>Point Mutation</term><term>Protein Binding</term><term>Protein Structure, Secondary</term><term>Protein Structure, Tertiary</term><term>Receptors, Virus (chemistry)</term><term>Receptors, Virus (genetics)</term><term>Receptors, Virus (metabolism)</term><term>SARS Virus (growth & development)</term><term>SARS Virus (isolation & purification)</term><term>SARS Virus (pathogenicity)</term><term>Sequence Analysis, DNA</term><term>Severe Acute Respiratory Syndrome (epidemiology)</term><term>Severe Acute Respiratory Syndrome (etiology)</term><term>Severe Acute Respiratory Syndrome (pathology)</term><term>Spike Glycoprotein, Coronavirus</term><term>Templates, Genetic</term><term>Transfection</term><term>Vero Cells</term><term>Viral Envelope Proteins (chemistry)</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (metabolism)</term><term>Viverridae (virology)</term><term>Zoonoses (epidemiology)</term><term>Zoonoses (transmission)</term><term>Zoonoses (virology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Animaux</term><term>Anticorps monoclonaux (immunologie)</term><term>Cellules Vero</term><term>Chine (épidémiologie)</term><term>Chiroptera (virologie)</term><term>Cinétique</term><term>Données de séquences moléculaires</term><term>Effet cytopathogène viral</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires ()</term><term>Glycoprotéines membranaires (génétique)</term><term>Glycoprotéines membranaires (métabolisme)</term><term>Gènes viraux</term><term>Humains</term><term>Liaison aux protéines</term><term>Lignée cellulaire</term><term>Matrices (génétique)</term><term>Modèles moléculaires</term><term>Mutation ponctuelle</term><term>Plasmides</term><term>Protéines de l'enveloppe virale ()</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Protéines de l'enveloppe virale (métabolisme)</term><term>Protéines à fluorescence verte (métabolisme)</term><term>Récepteurs viraux ()</term><term>Récepteurs viraux (génétique)</term><term>Récepteurs viraux (métabolisme)</term><term>Réservoirs d'agents pathogènes</term><term>Structure secondaire des protéines</term><term>Structure tertiaire des protéines</term><term>Syndrome respiratoire aigu sévère (anatomopathologie)</term><term>Syndrome respiratoire aigu sévère (épidémiologie)</term><term>Syndrome respiratoire aigu sévère (étiologie)</term><term>Séquence d'acides aminés</term><term>Tests de neutralisation</term><term>Transfection</term><term>Virus du SRAS (croissance et développement)</term><term>Virus du SRAS (isolement et purification)</term><term>Virus du SRAS (pathogénicité)</term><term>Viverridae (virologie)</term><term>Zoonoses (transmission)</term><term>Zoonoses (virologie)</term><term>Zoonoses (épidémiologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="chemistry" 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SRAS</term></keywords><keywords scheme="MESH" qualifier="epidemiology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term><term>Zoonoses</term></keywords><keywords scheme="MESH" qualifier="etiology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="growth & development" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Récepteurs viraux</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Anticorps monoclonaux</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glycoprotéines membranaires</term><term>Protéines de l'enveloppe virale</term><term>Protéines à fluorescence verte</term><term>Récepteurs viraux</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="pathology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="transmission" xml:lang="en"><term>Zoonoses</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Chiroptera</term><term>Viverridae</term><term>Zoonoses</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Chiroptera</term><term>Viverridae</term><term>Zoonoses</term></keywords><keywords scheme="MESH" qualifier="épidémiologie" xml:lang="fr"><term>Chine</term><term>Syndrome respiratoire aigu sévère</term><term>Zoonoses</term></keywords><keywords scheme="MESH" qualifier="étiologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term><term>Animals</term><term>Cell Line</term><term>Chlorocebus aethiops</term><term>Cytopathogenic Effect, Viral</term><term>Disease Reservoirs</term><term>Genes, Viral</term><term>Humans</term><term>Kinetics</term><term>Models, Molecular</term><term>Molecular Sequence Data</term><term>Neutralization Tests</term><term>Plasmids</term><term>Point Mutation</term><term>Protein Binding</term><term>Protein Structure, Secondary</term><term>Protein Structure, Tertiary</term><term>Sequence Analysis, DNA</term><term>Spike Glycoprotein, Coronavirus</term><term>Templates, Genetic</term><term>Transfection</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term><term>Animaux</term><term>Cellules Vero</term><term>Cinétique</term><term>Données de séquences moléculaires</term><term>Effet cytopathogène viral</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires</term><term>Gènes viraux</term><term>Humains</term><term>Liaison aux protéines</term><term>Lignée cellulaire</term><term>Matrices (génétique)</term><term>Modèles moléculaires</term><term>Mutation ponctuelle</term><term>Plasmides</term><term>Protéines de l'enveloppe virale</term><term>Récepteurs viraux</term><term>Réservoirs d'agents pathogènes</term><term>Structure secondaire des protéines</term><term>Structure tertiaire des protéines</term><term>Séquence d'acides aminés</term><term>Tests de neutralisation</term><term>Transfection</term></keywords><keywords scheme="Wicri" type="geographic" xml:lang="fr"><term>République populaire de Chine</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>Zoonotic severe acute respiratory syndrome coronavirus (SARS-CoV) likely evolved to infect humans by a series of transmission events between humans and animals in markets in China. Virus sequence data suggest that the palm civet served as an amplification host in which civet and human interaction fostered the evolution of the epidemic SARS Urbani strain. The prototypic civet strain of SARS-CoV, SZ16, was isolated from a palm civet but has not been successfully cultured in vitro. To propagate a chimeric recombinant SARS-CoV bearing an SZ16 spike (S) glycoprotein (icSZ16-S), we constructed cell lines expressing the civet ortholog (DBT-cACE2) of the SARS-CoV receptor (hACE2). Zoonotic SARS-CoV was completely dependent on ACE2 for entry. Urbani grew with similar kinetics in both the DBT-cACE2 and the DBT-hACE2 cells, while icSZ16-S only grew in DBT-cACE2 cells. The SZ16-S mutant viruses adapted to human airway epithelial cells and displayed enhanced affinity for hACE2 but exhibited severe growth defects in the DBT-cACE2 cells, suggesting that the evolutionary pathway that promoted efficient hACE2 interactions simultaneously abolished efficient cACE2 interactions. Structural modeling predicted two distinct biochemical interaction networks by which zoonotic receptor binding domain architecture can productively engage hACE2, but only the Urbani mutational repertoire promoted efficient usage of both hACE2 and cACE2 binding interfaces. Since dual species tropism was preserved in Urbani, it is likely that the virus evolved a high affinity for cACE2/hACE2 receptors through adaptation via repeated passages between human and civet hosts. Furthermore, zoonotic SARS-CoV was variably neutralized by antibodies that were effective against the epidemic strain, highlighting their utility for evaluating passive immunization efficacy.</p></div></front></TEI><affiliations><list></list><tree><noCountry><name sortKey="Baric, Ralph" sort="Baric, Ralph" uniqKey="Baric R" first="Ralph" last="Baric">Ralph Baric</name><name sortKey="Corti, Davide" sort="Corti, Davide" uniqKey="Corti D" first="Davide" last="Corti">Davide Corti</name><name sortKey="Donaldson, Eric" sort="Donaldson, Eric" uniqKey="Donaldson E" first="Eric" last="Donaldson">Eric Donaldson</name><name sortKey="Rockx, Barry" sort="Rockx, Barry" uniqKey="Rockx B" first="Barry" last="Rockx">Barry Rockx</name><name sortKey="Sheahan, Timothy" sort="Sheahan, Timothy" uniqKey="Sheahan T" first="Timothy" last="Sheahan">Timothy Sheahan</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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