Preparation of armored RNA as a control for multiplex real-time reverse transcription-PCR detection of influenza virus and severe acute respiratory syndrome coronavirus.
Identifieur interne : 001B52 ( Ncbi/Checkpoint ); précédent : 001B51; suivant : 001B53Preparation of armored RNA as a control for multiplex real-time reverse transcription-PCR detection of influenza virus and severe acute respiratory syndrome coronavirus.
Auteurs : Xin-Fen Yu [République populaire de Chine] ; Jing-Cao Pan ; Rong Ye ; Hai-Qing Xiang ; Yu Kou ; Zhi-Cheng HuangSource :
- Journal of clinical microbiology [ 1098-660X ] ; 2008.
Descripteurs français
- KwdFr :
- ARN viral (analyse), ARN viral (génétique), Assemblage viral, Données de séquences moléculaires, Escherichia coli (génétique), Escherichia coli (métabolisme), Escherichia coli (virologie), Grippe humaine (diagnostic), Humains, Levivirus (génétique), Levivirus (métabolisme), Normes de référence, RT-PCR (), RT-PCR (normes), Syndrome respiratoire aigu sévère (diagnostic), Séquence nucléotidique, Virus de la grippe A (génétique), Virus de la grippe A (isolement et purification), Virus du SRAS (génétique), Virus du SRAS (isolement et purification), Virus influenza B (génétique), Virus influenza B (isolement et purification).
- MESH :
- analyse : ARN viral.
- diagnostic : Grippe humaine, Syndrome respiratoire aigu sévère.
- génétique : ARN viral, Escherichia coli, Levivirus, Virus de la grippe A, Virus du SRAS, Virus influenza B.
- isolement et purification : Virus de la grippe A, Virus du SRAS, Virus influenza B.
- métabolisme : Escherichia coli, Levivirus.
- normes : RT-PCR.
- virologie : Escherichia coli.
- Assemblage viral, Données de séquences moléculaires, Humains, Normes de référence, RT-PCR, Séquence nucléotidique.
English descriptors
- KwdEn :
- Base Sequence, Escherichia coli (genetics), Escherichia coli (metabolism), Escherichia coli (virology), Humans, Influenza A virus (genetics), Influenza A virus (isolation & purification), Influenza B virus (genetics), Influenza B virus (isolation & purification), Influenza, Human (diagnosis), Levivirus (genetics), Levivirus (metabolism), Molecular Sequence Data, RNA, Viral (analysis), RNA, Viral (genetics), Reference Standards, Reverse Transcriptase Polymerase Chain Reaction (methods), Reverse Transcriptase Polymerase Chain Reaction (standards), SARS Virus (genetics), SARS Virus (isolation & purification), Severe Acute Respiratory Syndrome (diagnosis), Virus Assembly.
- MESH :
- chemical , analysis : RNA, Viral.
- diagnosis : Influenza, Human, Severe Acute Respiratory Syndrome.
- genetics : Escherichia coli, Influenza A virus, Influenza B virus, Levivirus, RNA, Viral, SARS Virus.
- isolation & purification : Influenza A virus, Influenza B virus, SARS Virus.
- metabolism : Escherichia coli, Levivirus.
- methods : Reverse Transcriptase Polymerase Chain Reaction.
- standards : Reverse Transcriptase Polymerase Chain Reaction.
- virology : Escherichia coli.
- Base Sequence, Humans, Molecular Sequence Data, Reference Standards, Virus Assembly.
Abstract
The common respiratory viruses, including influenza A, influenza B, and newly emerging severe acute respiratory syndrome (SARS) viruses, may cause similar clinical symptoms. Therefore, differential diagnosis of these virus pathogens is frequently required for single clinical samples. In addition, there is an urgent need for noninfectious and stable RNA standards and controls for multivirus detection. In this study, reverse transcription-PCR (RT-PCR) targeting of the RNAs of influenza A and influenza B viruses and SARS coronavirus was performed, and the resulting products were spliced into a fragment which was packaged into armored RNA for use as a noninfectious, quantifiable synthetic substitute. Furthermore, in the present study we developed a multiplex real-time RT-PCR assay in which the armored RNA was used as an external positive control and the three RNA viruses could be detected simultaneously in a single reaction mix. The detection limit of the multiplex real-time PCR was 10 copies/microl of armored RNA.
DOI: 10.1128/JCM.01904-07
PubMed: 18160451
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 001C45
- to stream PubMed, to step Curation: 001C45
- to stream PubMed, to step Checkpoint: 001A60
- to stream Ncbi, to step Merge: 001B52
- to stream Ncbi, to step Curation: 001B52
Links to Exploration step
pubmed:18160451Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Preparation of armored RNA as a control for multiplex real-time reverse transcription-PCR detection of influenza virus and severe acute respiratory syndrome coronavirus.</title><author><name sortKey="Yu, Xin Fen" sort="Yu, Xin Fen" uniqKey="Yu X" first="Xin-Fen" last="Yu">Xin-Fen Yu</name><affiliation wicri:level="3"><nlm:affiliation>Microbiology Laboratory, Hangzhou Center for Disease Control and Prevention, No. 277, Wu Lin Road, Hangzhou, People's Republic of China. yuxinfen@hotmail.com</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Microbiology Laboratory, Hangzhou Center for Disease Control and Prevention, No. 277, Wu Lin Road, Hangzhou</wicri:regionArea><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName></affiliation></author><author><name sortKey="Pan, Jing Cao" sort="Pan, Jing Cao" uniqKey="Pan J" first="Jing-Cao" last="Pan">Jing-Cao Pan</name></author><author><name sortKey="Ye, Rong" sort="Ye, Rong" uniqKey="Ye R" first="Rong" last="Ye">Rong Ye</name></author><author><name sortKey="Xiang, Hai Qing" sort="Xiang, Hai Qing" uniqKey="Xiang H" first="Hai-Qing" last="Xiang">Hai-Qing Xiang</name></author><author><name sortKey="Kou, Yu" sort="Kou, Yu" uniqKey="Kou Y" first="Yu" last="Kou">Yu Kou</name></author><author><name sortKey="Huang, Zhi Cheng" sort="Huang, Zhi Cheng" uniqKey="Huang Z" first="Zhi-Cheng" last="Huang">Zhi-Cheng Huang</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2008">2008</date><idno type="RBID">pubmed:18160451</idno><idno type="pmid">18160451</idno><idno type="doi">10.1128/JCM.01904-07</idno><idno type="wicri:Area/PubMed/Corpus">001C45</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">001C45</idno><idno type="wicri:Area/PubMed/Curation">001C45</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">001C45</idno><idno type="wicri:Area/PubMed/Checkpoint">001A60</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">001A60</idno><idno type="wicri:Area/Ncbi/Merge">001B52</idno><idno type="wicri:Area/Ncbi/Curation">001B52</idno><idno type="wicri:Area/Ncbi/Checkpoint">001B52</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Preparation of armored RNA as a control for multiplex real-time reverse transcription-PCR detection of influenza virus and severe acute respiratory syndrome coronavirus.</title><author><name sortKey="Yu, Xin Fen" sort="Yu, Xin Fen" uniqKey="Yu X" first="Xin-Fen" last="Yu">Xin-Fen Yu</name><affiliation wicri:level="3"><nlm:affiliation>Microbiology Laboratory, Hangzhou Center for Disease Control and Prevention, No. 277, Wu Lin Road, Hangzhou, People's Republic of China. yuxinfen@hotmail.com</nlm:affiliation><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Microbiology Laboratory, Hangzhou Center for Disease Control and Prevention, No. 277, Wu Lin Road, Hangzhou</wicri:regionArea><placeName><settlement type="city">Hangzhou</settlement><region type="province">Zhejiang</region></placeName></affiliation></author><author><name sortKey="Pan, Jing Cao" sort="Pan, Jing Cao" uniqKey="Pan J" first="Jing-Cao" last="Pan">Jing-Cao Pan</name></author><author><name sortKey="Ye, Rong" sort="Ye, Rong" uniqKey="Ye R" first="Rong" last="Ye">Rong Ye</name></author><author><name sortKey="Xiang, Hai Qing" sort="Xiang, Hai Qing" uniqKey="Xiang H" first="Hai-Qing" last="Xiang">Hai-Qing Xiang</name></author><author><name sortKey="Kou, Yu" sort="Kou, Yu" uniqKey="Kou Y" first="Yu" last="Kou">Yu Kou</name></author><author><name sortKey="Huang, Zhi Cheng" sort="Huang, Zhi Cheng" uniqKey="Huang Z" first="Zhi-Cheng" last="Huang">Zhi-Cheng Huang</name></author></analytic><series><title level="j">Journal of clinical microbiology</title><idno type="eISSN">1098-660X</idno><imprint><date when="2008" type="published">2008</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Base Sequence</term><term>Escherichia coli (genetics)</term><term>Escherichia coli (metabolism)</term><term>Escherichia coli (virology)</term><term>Humans</term><term>Influenza A virus (genetics)</term><term>Influenza A virus (isolation & purification)</term><term>Influenza B virus (genetics)</term><term>Influenza B virus (isolation & purification)</term><term>Influenza, Human (diagnosis)</term><term>Levivirus (genetics)</term><term>Levivirus (metabolism)</term><term>Molecular Sequence Data</term><term>RNA, Viral (analysis)</term><term>RNA, Viral (genetics)</term><term>Reference Standards</term><term>Reverse Transcriptase Polymerase Chain Reaction (methods)</term><term>Reverse Transcriptase Polymerase Chain Reaction (standards)</term><term>SARS Virus (genetics)</term><term>SARS Virus (isolation & purification)</term><term>Severe Acute Respiratory Syndrome (diagnosis)</term><term>Virus Assembly</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>ARN viral (analyse)</term><term>ARN viral (génétique)</term><term>Assemblage viral</term><term>Données de séquences moléculaires</term><term>Escherichia coli (génétique)</term><term>Escherichia coli (métabolisme)</term><term>Escherichia coli (virologie)</term><term>Grippe humaine (diagnostic)</term><term>Humains</term><term>Levivirus (génétique)</term><term>Levivirus (métabolisme)</term><term>Normes de référence</term><term>RT-PCR ()</term><term>RT-PCR (normes)</term><term>Syndrome respiratoire aigu sévère (diagnostic)</term><term>Séquence nucléotidique</term><term>Virus de la grippe A (génétique)</term><term>Virus de la grippe A (isolement et purification)</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (isolement et purification)</term><term>Virus influenza B (génétique)</term><term>Virus influenza B (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>RNA, Viral</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>ARN viral</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Influenza, Human</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Grippe humaine</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Escherichia coli</term><term>Influenza A virus</term><term>Influenza B virus</term><term>Levivirus</term><term>RNA, Viral</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>ARN viral</term><term>Escherichia coli</term><term>Levivirus</term><term>Virus de la grippe A</term><term>Virus du SRAS</term><term>Virus influenza B</term></keywords><keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en"><term>Influenza A virus</term><term>Influenza B virus</term><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="isolement et purification" xml:lang="fr"><term>Virus de la grippe A</term><term>Virus du SRAS</term><term>Virus influenza B</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Escherichia coli</term><term>Levivirus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Escherichia coli</term><term>Levivirus</term></keywords><keywords scheme="MESH" qualifier="normes" xml:lang="fr"><term>RT-PCR</term></keywords><keywords scheme="MESH" qualifier="standards" xml:lang="en"><term>Reverse Transcriptase Polymerase Chain Reaction</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Escherichia coli</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Escherichia coli</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Base Sequence</term><term>Humans</term><term>Molecular Sequence Data</term><term>Reference Standards</term><term>Virus Assembly</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Assemblage viral</term><term>Données de séquences moléculaires</term><term>Humains</term><term>Normes de référence</term><term>RT-PCR</term><term>Séquence nucléotidique</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">The common respiratory viruses, including influenza A, influenza B, and newly emerging severe acute respiratory syndrome (SARS) viruses, may cause similar clinical symptoms. Therefore, differential diagnosis of these virus pathogens is frequently required for single clinical samples. In addition, there is an urgent need for noninfectious and stable RNA standards and controls for multivirus detection. In this study, reverse transcription-PCR (RT-PCR) targeting of the RNAs of influenza A and influenza B viruses and SARS coronavirus was performed, and the resulting products were spliced into a fragment which was packaged into armored RNA for use as a noninfectious, quantifiable synthetic substitute. Furthermore, in the present study we developed a multiplex real-time RT-PCR assay in which the armored RNA was used as an external positive control and the three RNA viruses could be detected simultaneously in a single reaction mix. The detection limit of the multiplex real-time PCR was 10 copies/microl of armored RNA.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country><region><li>Zhejiang</li></region><settlement><li>Hangzhou</li></settlement></list><tree><noCountry><name sortKey="Huang, Zhi Cheng" sort="Huang, Zhi Cheng" uniqKey="Huang Z" first="Zhi-Cheng" last="Huang">Zhi-Cheng Huang</name><name sortKey="Kou, Yu" sort="Kou, Yu" uniqKey="Kou Y" first="Yu" last="Kou">Yu Kou</name><name sortKey="Pan, Jing Cao" sort="Pan, Jing Cao" uniqKey="Pan J" first="Jing-Cao" last="Pan">Jing-Cao Pan</name><name sortKey="Xiang, Hai Qing" sort="Xiang, Hai Qing" uniqKey="Xiang H" first="Hai-Qing" last="Xiang">Hai-Qing Xiang</name><name sortKey="Ye, Rong" sort="Ye, Rong" uniqKey="Ye R" first="Rong" last="Ye">Rong Ye</name></noCountry><country name="République populaire de Chine"><region name="Zhejiang"><name sortKey="Yu, Xin Fen" sort="Yu, Xin Fen" uniqKey="Yu X" first="Xin-Fen" last="Yu">Xin-Fen Yu</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Ncbi/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001B52 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd -nk 001B52 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Ncbi
|étape= Checkpoint
|type= RBID
|clé= pubmed:18160451
|texte= Preparation of armored RNA as a control for multiplex real-time reverse transcription-PCR detection of influenza virus and severe acute respiratory syndrome coronavirus.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/RBID.i -Sk "pubmed:18160451" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |