Intracellular localization of the SARS coronavirus protein 9b: evidence of active export from the nucleus.
Identifieur interne : 001932 ( Ncbi/Checkpoint ); précédent : 001931; suivant : 001933Intracellular localization of the SARS coronavirus protein 9b: evidence of active export from the nucleus.
Auteurs : Igor Moshynskyy [Canada] ; Sathiyanarayanan Viswanathan ; Natalia Vasilenko ; Vladislav Lobanov ; Martin Petric ; Lorne A. Babiuk ; Alexander N. ZakhartchoukSource :
- Virus research [ 0168-1702 ] ; 2007.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (métabolisme), Cellules Vero, Lapins, Lignée cellulaire, Noyau de la cellule (virologie), Protéines de capside (génétique), Protéines de capside (métabolisme), Signaux de localisation nucléaire (métabolisme), Technique d'immunofluorescence directe, Transport biologique, Virus du SRAS (), Virus du SRAS (génétique), Virus du SRAS (immunologie), Virus du SRAS (métabolisme).
- MESH :
- génétique : Protéines de capside, Virus du SRAS.
- immunologie : Virus du SRAS.
- métabolisme : Anticorps antiviraux, Protéines de capside, Signaux de localisation nucléaire, Virus du SRAS.
- virologie : Noyau de la cellule.
- Animaux, Cellules Vero, Lapins, Lignée cellulaire, Technique d'immunofluorescence directe, Transport biologique, Virus du SRAS.
English descriptors
- KwdEn :
- Animals, Antibodies, Viral (metabolism), Biological Transport, Capsid Proteins (genetics), Capsid Proteins (metabolism), Cell Line, Cell Nucleus (virology), Chlorocebus aethiops, Fluorescent Antibody Technique, Direct, Nuclear Localization Signals (metabolism), Rabbits, SARS Virus (chemistry), SARS Virus (genetics), SARS Virus (immunology), SARS Virus (metabolism), Vero Cells.
- MESH :
- chemical , genetics : Capsid Proteins.
- chemical , metabolism : Antibodies, Viral, Capsid Proteins, Nuclear Localization Signals.
- chemistry : SARS Virus.
- genetics : SARS Virus.
- immunology : SARS Virus.
- metabolism : SARS Virus.
- virology : Cell Nucleus.
- Animals, Biological Transport, Cell Line, Chlorocebus aethiops, Fluorescent Antibody Technique, Direct, Rabbits, Vero Cells.
Abstract
Open reading frame 9b (ORF 9b) encodes a 98 amino acid group-specific protein of severe acute respiratory syndrome (SARS) coronavirus (CoV). It has no homology with known proteins and its function in SARS CoV replication has not been determined. The N-terminal part of the 9b protein was used to raise polyclonal antibodies in rabbits, and these antibodies could detect 9b protein in infected cells. We analyzed the sub-cellular localization of recombinant 9b protein using fluorescence microscopy of live transfected cells and indirect immunofluorescence of transfected fixed cells. Our findings indicate that the 9b protein is exported outside of a cell nucleus and localizes to the endoplasmic reticulum. Our data also suggest that the 46-LRLGSQLSL-54 amino acid sequence of 9b functions as a nuclear export signal (NES).
DOI: 10.1016/j.virusres.2007.03.011
PubMed: 17448558
Affiliations:
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pubmed:17448558Le document en format XML
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Babiuk</name></author><author><name sortKey="Zakhartchouk, Alexander N" sort="Zakhartchouk, Alexander N" uniqKey="Zakhartchouk A" first="Alexander N" last="Zakhartchouk">Alexander N. Zakhartchouk</name></author></analytic><series><title level="j">Virus research</title><idno type="ISSN">0168-1702</idno><imprint><date when="2007" type="published">2007</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Viral (metabolism)</term><term>Biological Transport</term><term>Capsid Proteins (genetics)</term><term>Capsid Proteins (metabolism)</term><term>Cell Line</term><term>Cell Nucleus (virology)</term><term>Chlorocebus aethiops</term><term>Fluorescent Antibody Technique, Direct</term><term>Nuclear Localization Signals (metabolism)</term><term>Rabbits</term><term>SARS Virus (chemistry)</term><term>SARS Virus (genetics)</term><term>SARS Virus (immunology)</term><term>SARS Virus (metabolism)</term><term>Vero Cells</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antiviraux (métabolisme)</term><term>Cellules Vero</term><term>Lapins</term><term>Lignée cellulaire</term><term>Noyau de la cellule (virologie)</term><term>Protéines de capside (génétique)</term><term>Protéines de capside (métabolisme)</term><term>Signaux de localisation nucléaire (métabolisme)</term><term>Technique d'immunofluorescence directe</term><term>Transport biologique</term><term>Virus du SRAS ()</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (immunologie)</term><term>Virus du SRAS (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Capsid Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Antibodies, Viral</term><term>Capsid Proteins</term><term>Nuclear Localization Signals</term></keywords><keywords scheme="MESH" qualifier="chemistry" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines de capside</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Anticorps antiviraux</term><term>Protéines de capside</term><term>Signaux de localisation nucléaire</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Noyau de la cellule</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Cell Nucleus</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Biological Transport</term><term>Cell Line</term><term>Chlorocebus aethiops</term><term>Fluorescent Antibody Technique, Direct</term><term>Rabbits</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Cellules Vero</term><term>Lapins</term><term>Lignée cellulaire</term><term>Technique d'immunofluorescence directe</term><term>Transport biologique</term><term>Virus du SRAS</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Open reading frame 9b (ORF 9b) encodes a 98 amino acid group-specific protein of severe acute respiratory syndrome (SARS) coronavirus (CoV). It has no homology with known proteins and its function in SARS CoV replication has not been determined. The N-terminal part of the 9b protein was used to raise polyclonal antibodies in rabbits, and these antibodies could detect 9b protein in infected cells. We analyzed the sub-cellular localization of recombinant 9b protein using fluorescence microscopy of live transfected cells and indirect immunofluorescence of transfected fixed cells. Our findings indicate that the 9b protein is exported outside of a cell nucleus and localizes to the endoplasmic reticulum. Our data also suggest that the 46-LRLGSQLSL-54 amino acid sequence of 9b functions as a nuclear export signal (NES).</div></front></TEI><affiliations><list><country><li>Canada</li></country></list><tree><noCountry><name sortKey="Babiuk, Lorne A" sort="Babiuk, Lorne A" uniqKey="Babiuk L" first="Lorne A" last="Babiuk">Lorne A. Babiuk</name><name sortKey="Lobanov, Vladislav" sort="Lobanov, Vladislav" uniqKey="Lobanov V" first="Vladislav" last="Lobanov">Vladislav Lobanov</name><name sortKey="Petric, Martin" sort="Petric, Martin" uniqKey="Petric M" first="Martin" last="Petric">Martin Petric</name><name sortKey="Vasilenko, Natalia" sort="Vasilenko, Natalia" uniqKey="Vasilenko N" first="Natalia" last="Vasilenko">Natalia Vasilenko</name><name sortKey="Viswanathan, Sathiyanarayanan" sort="Viswanathan, Sathiyanarayanan" uniqKey="Viswanathan S" first="Sathiyanarayanan" last="Viswanathan">Sathiyanarayanan Viswanathan</name><name sortKey="Zakhartchouk, Alexander N" sort="Zakhartchouk, Alexander N" uniqKey="Zakhartchouk A" first="Alexander N" last="Zakhartchouk">Alexander N. Zakhartchouk</name></noCountry><country name="Canada"><noRegion><name sortKey="Moshynskyy, Igor" sort="Moshynskyy, Igor" uniqKey="Moshynskyy I" first="Igor" last="Moshynskyy">Igor Moshynskyy</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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