Inhibition of cell proliferation by SARS-CoV infection in Vero E6 cells.
Identifieur interne : 001384 ( Ncbi/Checkpoint ); précédent : 001383; suivant : 001385Inhibition of cell proliferation by SARS-CoV infection in Vero E6 cells.
Auteurs : Tetsuya Mizutani [Japon] ; Shuetsu Fukushi ; Daisuke Iizuka ; Osamu Inanami ; Mikinori Kuwabara ; Hideaki Takashima ; Hiroshi Yanagawa ; Masayuki Saijo ; Ichiro Kurane ; Shigeru MorikawaSource :
- FEMS immunology and medical microbiology [ 0928-8244 ] ; 2006.
Descripteurs français
- KwdFr :
- Animaux, Apoptose, Cellules Vero, Glycogen Synthase Kinase 3 (génétique), Glycogen Synthase Kinase 3 (métabolisme), Humains, Phosphatidylinositol 3-kinases (génétique), Phosphatidylinositol 3-kinases (métabolisme), Phosphorylation, Prolifération cellulaire, Protéines proto-oncogènes c-akt (génétique), Protéines proto-oncogènes c-akt (métabolisme), Régulation de l'expression des gènes, Transduction du signal, Virus du SRAS (pathogénicité).
- MESH :
- génétique : Glycogen Synthase Kinase 3, Phosphatidylinositol 3-kinases, Protéines proto-oncogènes c-akt.
- métabolisme : Glycogen Synthase Kinase 3, Phosphatidylinositol 3-kinases, Protéines proto-oncogènes c-akt.
- pathogénicité : Virus du SRAS.
- Animaux, Apoptose, Cellules Vero, Humains, Phosphorylation, Prolifération cellulaire, Régulation de l'expression des gènes, Transduction du signal.
English descriptors
- KwdEn :
- Animals, Apoptosis, Cell Proliferation, Chlorocebus aethiops, Gene Expression Regulation, Glycogen Synthase Kinase 3 (genetics), Glycogen Synthase Kinase 3 (metabolism), Humans, Phosphatidylinositol 3-Kinases (genetics), Phosphatidylinositol 3-Kinases (metabolism), Phosphorylation, Proto-Oncogene Proteins c-akt (genetics), Proto-Oncogene Proteins c-akt (metabolism), SARS Virus (pathogenicity), Signal Transduction, Vero Cells.
- MESH :
- chemical , genetics : Glycogen Synthase Kinase 3, Proto-Oncogene Proteins c-akt.
- chemical , metabolism : Glycogen Synthase Kinase 3, Proto-Oncogene Proteins c-akt.
- genetics : Phosphatidylinositol 3-Kinases.
- metabolism : Phosphatidylinositol 3-Kinases.
- pathogenicity : SARS Virus.
- Animals, Apoptosis, Cell Proliferation, Chlorocebus aethiops, Gene Expression Regulation, Humans, Phosphorylation, Signal Transduction, Vero Cells.
Abstract
Severe acute respiratory syndrome (SARS) is caused by SARS-coronavirus (SARS-CoV). Infection of Vero E6 cells with SARS-CoV inhibits cell proliferation. Our previous study indicated that Akt, which is poorly phosphorylated in confluent cultures of Vero E6 cells, is phosphorylated and then dephosphorylated upon infection by SARS-CoV. In the present study, we showed that a serine residue of Akt was phosphorylated in Vero E6 cells in subconfluent culture and that Akt was dephosphorylated rapidly after SARS-CoV infection without up-regulation of its phosphorylation. Phosphorylation of glycogen synthase kinase-3beta, which is one of the downstream targets of Akt, was prevented in SARS-CoV-infected cells. However, treatment with glycogen synthase kinase-3beta small interfering RNA indicated that the glycogen synthase kinase-3beta signaling pathway was not related to inhibition of cell proliferation. Treatment of Vero E6 cells with the phosphatidylinositol 3'-kinase/Akt inhibitor, LY294002, which induces dephosphorylation of Akt, inhibited cell proliferation. As shown in our previous studies, apoptosis occurred in virus-infected cells within 18 h postinfection. Cellular mRNA transcription, which was reported to be up-regulated in SARS-CoV-infected Caco-2 cells, was not up-regulated in virus-infected Vero E6 cells, partially as a result of apoptosis. These results suggested that inhibition of cell proliferation is regulated by both the phosphatidylinositol 3'-kinase/Akt signaling pathway and by apoptosis in SARS-CoV-infected Vero E6 cells. This is the first study to analyze SARS-CoV-induced cell growth inhibition.
DOI: 10.1111/j.1574-695X.2005.00028.x
PubMed: 16487305
Affiliations:
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 002339
- to stream PubMed, to step Curation: 002339
- to stream PubMed, to step Checkpoint: 002138
- to stream Ncbi, to step Merge: 001384
- to stream Ncbi, to step Curation: 001384
Links to Exploration step
pubmed:16487305Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Inhibition of cell proliferation by SARS-CoV infection in Vero E6 cells.</title><author><name sortKey="Mizutani, Tetsuya" sort="Mizutani, Tetsuya" uniqKey="Mizutani T" first="Tetsuya" last="Mizutani">Tetsuya Mizutani</name><affiliation wicri:level="3"><nlm:affiliation>Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Tokyo, Japan. tmizutan@nih.go.jp</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Fukushi, Shuetsu" sort="Fukushi, Shuetsu" uniqKey="Fukushi S" first="Shuetsu" last="Fukushi">Shuetsu Fukushi</name></author><author><name sortKey="Iizuka, Daisuke" sort="Iizuka, Daisuke" uniqKey="Iizuka D" first="Daisuke" last="Iizuka">Daisuke Iizuka</name></author><author><name sortKey="Inanami, Osamu" sort="Inanami, Osamu" uniqKey="Inanami O" first="Osamu" last="Inanami">Osamu Inanami</name></author><author><name sortKey="Kuwabara, Mikinori" sort="Kuwabara, Mikinori" uniqKey="Kuwabara M" first="Mikinori" last="Kuwabara">Mikinori Kuwabara</name></author><author><name sortKey="Takashima, Hideaki" sort="Takashima, Hideaki" uniqKey="Takashima H" first="Hideaki" last="Takashima">Hideaki Takashima</name></author><author><name sortKey="Yanagawa, Hiroshi" sort="Yanagawa, Hiroshi" uniqKey="Yanagawa H" first="Hiroshi" last="Yanagawa">Hiroshi Yanagawa</name></author><author><name sortKey="Saijo, Masayuki" sort="Saijo, Masayuki" uniqKey="Saijo M" first="Masayuki" last="Saijo">Masayuki Saijo</name></author><author><name sortKey="Kurane, Ichiro" sort="Kurane, Ichiro" uniqKey="Kurane I" first="Ichiro" last="Kurane">Ichiro Kurane</name></author><author><name sortKey="Morikawa, Shigeru" sort="Morikawa, Shigeru" uniqKey="Morikawa S" first="Shigeru" last="Morikawa">Shigeru Morikawa</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="2006">2006</date><idno type="RBID">pubmed:16487305</idno><idno type="pmid">16487305</idno><idno type="doi">10.1111/j.1574-695X.2005.00028.x</idno><idno type="wicri:Area/PubMed/Corpus">002339</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">002339</idno><idno type="wicri:Area/PubMed/Curation">002339</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">002339</idno><idno type="wicri:Area/PubMed/Checkpoint">002138</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">002138</idno><idno type="wicri:Area/Ncbi/Merge">001384</idno><idno type="wicri:Area/Ncbi/Curation">001384</idno><idno type="wicri:Area/Ncbi/Checkpoint">001384</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Inhibition of cell proliferation by SARS-CoV infection in Vero E6 cells.</title><author><name sortKey="Mizutani, Tetsuya" sort="Mizutani, Tetsuya" uniqKey="Mizutani T" first="Tetsuya" last="Mizutani">Tetsuya Mizutani</name><affiliation wicri:level="3"><nlm:affiliation>Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Tokyo, Japan. tmizutan@nih.go.jp</nlm:affiliation><country xml:lang="fr">Japon</country><wicri:regionArea>Special Pathogens Laboratory, Department of Virology 1, National Institute of Infectious Diseases, Tokyo</wicri:regionArea><placeName><settlement type="city">Tokyo</settlement><region type="région">Région de Kantō</region></placeName></affiliation></author><author><name sortKey="Fukushi, Shuetsu" sort="Fukushi, Shuetsu" uniqKey="Fukushi S" first="Shuetsu" last="Fukushi">Shuetsu Fukushi</name></author><author><name sortKey="Iizuka, Daisuke" sort="Iizuka, Daisuke" uniqKey="Iizuka D" first="Daisuke" last="Iizuka">Daisuke Iizuka</name></author><author><name sortKey="Inanami, Osamu" sort="Inanami, Osamu" uniqKey="Inanami O" first="Osamu" last="Inanami">Osamu Inanami</name></author><author><name sortKey="Kuwabara, Mikinori" sort="Kuwabara, Mikinori" uniqKey="Kuwabara M" first="Mikinori" last="Kuwabara">Mikinori Kuwabara</name></author><author><name sortKey="Takashima, Hideaki" sort="Takashima, Hideaki" uniqKey="Takashima H" first="Hideaki" last="Takashima">Hideaki Takashima</name></author><author><name sortKey="Yanagawa, Hiroshi" sort="Yanagawa, Hiroshi" uniqKey="Yanagawa H" first="Hiroshi" last="Yanagawa">Hiroshi Yanagawa</name></author><author><name sortKey="Saijo, Masayuki" sort="Saijo, Masayuki" uniqKey="Saijo M" first="Masayuki" last="Saijo">Masayuki Saijo</name></author><author><name sortKey="Kurane, Ichiro" sort="Kurane, Ichiro" uniqKey="Kurane I" first="Ichiro" last="Kurane">Ichiro Kurane</name></author><author><name sortKey="Morikawa, Shigeru" sort="Morikawa, Shigeru" uniqKey="Morikawa S" first="Shigeru" last="Morikawa">Shigeru Morikawa</name></author></analytic><series><title level="j">FEMS immunology and medical microbiology</title><idno type="ISSN">0928-8244</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Apoptosis</term><term>Cell Proliferation</term><term>Chlorocebus aethiops</term><term>Gene Expression Regulation</term><term>Glycogen Synthase Kinase 3 (genetics)</term><term>Glycogen Synthase Kinase 3 (metabolism)</term><term>Humans</term><term>Phosphatidylinositol 3-Kinases (genetics)</term><term>Phosphatidylinositol 3-Kinases (metabolism)</term><term>Phosphorylation</term><term>Proto-Oncogene Proteins c-akt (genetics)</term><term>Proto-Oncogene Proteins c-akt (metabolism)</term><term>SARS Virus (pathogenicity)</term><term>Signal Transduction</term><term>Vero Cells</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Apoptose</term><term>Cellules Vero</term><term>Glycogen Synthase Kinase 3 (génétique)</term><term>Glycogen Synthase Kinase 3 (métabolisme)</term><term>Humains</term><term>Phosphatidylinositol 3-kinases (génétique)</term><term>Phosphatidylinositol 3-kinases (métabolisme)</term><term>Phosphorylation</term><term>Prolifération cellulaire</term><term>Protéines proto-oncogènes c-akt (génétique)</term><term>Protéines proto-oncogènes c-akt (métabolisme)</term><term>Régulation de l'expression des gènes</term><term>Transduction du signal</term><term>Virus du SRAS (pathogénicité)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Glycogen Synthase Kinase 3</term><term>Proto-Oncogene Proteins c-akt</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Glycogen Synthase Kinase 3</term><term>Proto-Oncogene Proteins c-akt</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Phosphatidylinositol 3-Kinases</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glycogen Synthase Kinase 3</term><term>Phosphatidylinositol 3-kinases</term><term>Protéines proto-oncogènes c-akt</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>Phosphatidylinositol 3-Kinases</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Glycogen Synthase Kinase 3</term><term>Phosphatidylinositol 3-kinases</term><term>Protéines proto-oncogènes c-akt</term></keywords><keywords scheme="MESH" qualifier="pathogenicity" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="pathogénicité" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Apoptosis</term><term>Cell Proliferation</term><term>Chlorocebus aethiops</term><term>Gene Expression Regulation</term><term>Humans</term><term>Phosphorylation</term><term>Signal Transduction</term><term>Vero Cells</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Apoptose</term><term>Cellules Vero</term><term>Humains</term><term>Phosphorylation</term><term>Prolifération cellulaire</term><term>Régulation de l'expression des gènes</term><term>Transduction du signal</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Severe acute respiratory syndrome (SARS) is caused by SARS-coronavirus (SARS-CoV). Infection of Vero E6 cells with SARS-CoV inhibits cell proliferation. Our previous study indicated that Akt, which is poorly phosphorylated in confluent cultures of Vero E6 cells, is phosphorylated and then dephosphorylated upon infection by SARS-CoV. In the present study, we showed that a serine residue of Akt was phosphorylated in Vero E6 cells in subconfluent culture and that Akt was dephosphorylated rapidly after SARS-CoV infection without up-regulation of its phosphorylation. Phosphorylation of glycogen synthase kinase-3beta, which is one of the downstream targets of Akt, was prevented in SARS-CoV-infected cells. However, treatment with glycogen synthase kinase-3beta small interfering RNA indicated that the glycogen synthase kinase-3beta signaling pathway was not related to inhibition of cell proliferation. Treatment of Vero E6 cells with the phosphatidylinositol 3'-kinase/Akt inhibitor, LY294002, which induces dephosphorylation of Akt, inhibited cell proliferation. As shown in our previous studies, apoptosis occurred in virus-infected cells within 18 h postinfection. Cellular mRNA transcription, which was reported to be up-regulated in SARS-CoV-infected Caco-2 cells, was not up-regulated in virus-infected Vero E6 cells, partially as a result of apoptosis. These results suggested that inhibition of cell proliferation is regulated by both the phosphatidylinositol 3'-kinase/Akt signaling pathway and by apoptosis in SARS-CoV-infected Vero E6 cells. This is the first study to analyze SARS-CoV-induced cell growth inhibition.</div></front></TEI><affiliations><list><country><li>Japon</li></country><region><li>Région de Kantō</li></region><settlement><li>Tokyo</li></settlement></list><tree><noCountry><name sortKey="Fukushi, Shuetsu" sort="Fukushi, Shuetsu" uniqKey="Fukushi S" first="Shuetsu" last="Fukushi">Shuetsu Fukushi</name><name sortKey="Iizuka, Daisuke" sort="Iizuka, Daisuke" uniqKey="Iizuka D" first="Daisuke" last="Iizuka">Daisuke Iizuka</name><name sortKey="Inanami, Osamu" sort="Inanami, Osamu" uniqKey="Inanami O" first="Osamu" last="Inanami">Osamu Inanami</name><name sortKey="Kurane, Ichiro" sort="Kurane, Ichiro" uniqKey="Kurane I" first="Ichiro" last="Kurane">Ichiro Kurane</name><name sortKey="Kuwabara, Mikinori" sort="Kuwabara, Mikinori" uniqKey="Kuwabara M" first="Mikinori" last="Kuwabara">Mikinori Kuwabara</name><name sortKey="Morikawa, Shigeru" sort="Morikawa, Shigeru" uniqKey="Morikawa S" first="Shigeru" last="Morikawa">Shigeru Morikawa</name><name sortKey="Saijo, Masayuki" sort="Saijo, Masayuki" uniqKey="Saijo M" first="Masayuki" last="Saijo">Masayuki Saijo</name><name sortKey="Takashima, Hideaki" sort="Takashima, Hideaki" uniqKey="Takashima H" first="Hideaki" last="Takashima">Hideaki Takashima</name><name sortKey="Yanagawa, Hiroshi" sort="Yanagawa, Hiroshi" uniqKey="Yanagawa H" first="Hiroshi" last="Yanagawa">Hiroshi Yanagawa</name></noCountry><country name="Japon"><region name="Région de Kantō"><name sortKey="Mizutani, Tetsuya" sort="Mizutani, Tetsuya" uniqKey="Mizutani T" first="Tetsuya" last="Mizutani">Tetsuya Mizutani</name></region></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Ncbi/Checkpoint
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 001384 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd -nk 001384 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Ncbi
|étape= Checkpoint
|type= RBID
|clé= pubmed:16487305
|texte= Inhibition of cell proliferation by SARS-CoV infection in Vero E6 cells.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Ncbi/Checkpoint/RBID.i -Sk "pubmed:16487305" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Ncbi/Checkpoint/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |