Using the TAP Component of the Antigen-Processing Machinery as a Molecular Adjuvant
Identifieur interne : 001328 ( Ncbi/Checkpoint ); précédent : 001327; suivant : 001329Using the TAP Component of the Antigen-Processing Machinery as a Molecular Adjuvant
Auteurs : Timothy Z. Vitalis [Canada] ; Qian-Jin Zhang [Canada] ; Judie Alimonti [Canada] ; Susan S. Chen [Canada] ; Genc Basha [Canada] ; Alex Moise [Canada] ; Jacqueline Tiong [Canada] ; Mei Mei Tian [Canada] ; Kyung Bok Choi [Canada] ; Douglas Waterfield [Canada] ; Andy Jeffries [Canada] ; Wilfred A. Jefferies [Canada]Source :
- PLoS Pathogens [ 1553-7366 ] ; 2005.
Abstract
We hypothesize that over-expression of transporters associated with antigen processing (TAP1 and TAP2), components of the major histocompatibility complex (MHC) class I antigen-processing pathway, enhances antigen-specific cytotoxic activity in response to viral infection. An expression system using recombinant vaccinia virus (VV) was used to over-express human TAP1 and TAP2 (VV-hTAP1,2) in normal mice. Mice coinfected with either vesicular stomatitis virus plus VV-hTAP1,2 or Sendai virus plus VV-hTAP1,2 increased cytotoxic lymphocyte (CTL) activity by at least 4-fold when compared to coinfections with a control vector, VV encoding the plasmid PJS-5. Coinfections with VV-hTAP1,2 increased virus-specific CTL precursors compared to control infections without VV-hTAP1,2. In an animal model of lethal viral challenge after vaccination, VV-hTAP1,2 provided protection against a lethal challenge of VV at doses 100-fold lower than control vector alone. Mechanistically, the total MHC class I antigen surface expression and the cross-presentation mechanism in spleen-derived dendritic cells was augmented by over-expression of TAP. Furthermore, VV-hTAP1,2 increases splenic TAP transport activity and endogenous antigen processing, thus rendering infected targets more susceptible to CTL recognition and subsequent killing. This is the first demonstration that over-expression of a component of the antigen-processing machinery increases endogenous antigen presentation and dendritic cell cross-presentation of exogenous antigens and may provide a novel and general approach for increasing immune responses against pathogens at low doses of vaccine inocula.
Url:
DOI: 10.1371/journal.ppat.0010036
PubMed: 16389301
PubMed Central: 1323471
Affiliations:
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Vitalis</name><affiliation wicri:level="1"><nlm:aff id="aff1"> The Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea> The Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia</wicri:regionArea><wicri:noRegion>British Columbia</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2"> Biomedical Research Centre, University of British Columbia, Vancouver, British Columbia, Canada</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea> Biomedical Research Centre, University of British Columbia, Vancouver, British Columbia</wicri:regionArea><wicri:noRegion>British Columbia</wicri:noRegion></affiliation><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Zhang, Qian Jin" sort="Zhang, Qian Jin" uniqKey="Zhang Q" first="Qian-Jin" last="Zhang">Qian-Jin Zhang</name><affiliation wicri:level="1"><nlm:aff id="aff1"> The Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea> The Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia</wicri:regionArea><wicri:noRegion>British Columbia</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2"> Biomedical Research Centre, University of British Columbia, Vancouver, British Columbia, Canada</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea> Biomedical Research Centre, University of British Columbia, Vancouver, British Columbia</wicri:regionArea><wicri:noRegion>British Columbia</wicri:noRegion></affiliation><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Alimonti, Judie" sort="Alimonti, Judie" uniqKey="Alimonti J" first="Judie" last="Alimonti">Judie Alimonti</name><affiliation wicri:level="1"><nlm:aff id="aff1"> The Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea> The Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia</wicri:regionArea><wicri:noRegion>British Columbia</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2"> Biomedical Research Centre, University of British Columbia, Vancouver, British Columbia, Canada</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea> Biomedical Research Centre, University of British Columbia, Vancouver, British Columbia</wicri:regionArea><wicri:noRegion>British Columbia</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff3"> Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, Canada</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea> Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia</wicri:regionArea><wicri:noRegion>British Columbia</wicri:noRegion></affiliation><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Chen, Susan S" sort="Chen, Susan S" uniqKey="Chen S" first="Susan S" last="Chen">Susan S. 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of Zoology, University of British Columbia, Vancouver, British Columbia</wicri:regionArea><wicri:noRegion>British Columbia</wicri:noRegion></affiliation><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Basha, Genc" sort="Basha, Genc" uniqKey="Basha G" first="Genc" last="Basha">Genc Basha</name><affiliation wicri:level="1"><nlm:aff id="aff1"> The Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea> The Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia</wicri:regionArea><wicri:noRegion>British Columbia</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2"> Biomedical Research Centre, University of British Columbia, Vancouver, British Columbia, Canada</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea> Biomedical Research Centre, University of British Columbia, Vancouver, 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Canada</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea> Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia</wicri:regionArea><wicri:noRegion>British Columbia</wicri:noRegion></affiliation><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Choi, Kyung Bok" sort="Choi, Kyung Bok" uniqKey="Choi K" first="Kyung Bok" last="Choi">Kyung Bok Choi</name><affiliation wicri:level="1"><nlm:aff id="aff1"> The Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea> The Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia</wicri:regionArea><wicri:noRegion>British Columbia</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2"> Biomedical Research Centre, University of British Columbia, Vancouver, British Columbia, Canada</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea> Biomedical Research Centre, University of British Columbia, Vancouver, British Columbia</wicri:regionArea><wicri:noRegion>British Columbia</wicri:noRegion></affiliation><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Waterfield, Douglas" sort="Waterfield, Douglas" uniqKey="Waterfield D" first="Douglas" last="Waterfield">Douglas Waterfield</name><affiliation wicri:level="1"><nlm:aff id="aff5"> Department of Oral Biology, Faculty of Dentistry, University of British Columbia, Vancouver, British Columbia, Canada</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea> Department of Oral Biology, Faculty of Dentistry, University of British Columbia, Vancouver, British Columbia</wicri:regionArea><wicri:noRegion>British Columbia</wicri:noRegion></affiliation><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Jeffries, Andy" sort="Jeffries, Andy" uniqKey="Jeffries A" first="Andy" last="Jeffries">Andy Jeffries</name><affiliation wicri:level="1"><nlm:aff id="aff1"> The Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea> The Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia</wicri:regionArea><wicri:noRegion>British Columbia</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2"> Biomedical Research Centre, University of British Columbia, Vancouver, British Columbia, Canada</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea> Biomedical Research Centre, University of British Columbia, Vancouver, British Columbia</wicri:regionArea><wicri:noRegion>British Columbia</wicri:noRegion></affiliation><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Jefferies, Wilfred A" sort="Jefferies, Wilfred A" uniqKey="Jefferies W" first="Wilfred A" last="Jefferies">Wilfred A. Jefferies</name><affiliation wicri:level="1"><nlm:aff id="aff1"> The Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia, Canada</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea> The Michael Smith Laboratories, University of British Columbia, Vancouver, British Columbia</wicri:regionArea><wicri:noRegion>British Columbia</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2"> Biomedical Research Centre, University of British Columbia, Vancouver, British Columbia, Canada</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea> Biomedical Research Centre, University of British Columbia, Vancouver, British Columbia</wicri:regionArea><wicri:noRegion>British Columbia</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff3"> Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia, Canada</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea> Department of Microbiology and Immunology, University of British Columbia, Vancouver, British Columbia</wicri:regionArea><wicri:noRegion>British Columbia</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff4"> Department of Zoology, University of British Columbia, Vancouver, British Columbia, Canada</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea> Department of Zoology, University of British Columbia, Vancouver, British Columbia</wicri:regionArea><wicri:noRegion>British Columbia</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff6"> Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia, Canada</nlm:aff><country xml:lang="fr">Canada</country><wicri:regionArea> Department of Medical Genetics, University of British Columbia, Vancouver, British Columbia</wicri:regionArea><wicri:noRegion>British Columbia</wicri:noRegion></affiliation><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author></analytic><series><title level="j">PLoS Pathogens</title><idno type="ISSN">1553-7366</idno><idno type="eISSN">1553-7374</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>We hypothesize that over-expression of transporters associated with antigen processing (TAP1 and TAP2), components of the major histocompatibility complex (MHC) class I antigen-processing pathway, enhances antigen-specific cytotoxic activity in response to viral infection. An expression system using recombinant vaccinia virus (VV) was used to over-express human TAP1 and TAP2 (VV-hTAP1,2) in normal mice. Mice coinfected with either vesicular stomatitis virus plus VV-hTAP1,2 or Sendai virus plus VV-hTAP1,2 increased cytotoxic lymphocyte (CTL) activity by at least 4-fold when compared to coinfections with a control vector, VV encoding the plasmid PJS-5. Coinfections with VV-hTAP1,2 increased virus-specific CTL precursors compared to control infections without VV-hTAP1,2. In an animal model of lethal viral challenge after vaccination, VV-hTAP1,2 provided protection against a lethal challenge of VV at doses 100-fold lower than control vector alone. Mechanistically, the total MHC class I antigen surface expression and the cross-presentation mechanism in spleen-derived dendritic cells was augmented by over-expression of TAP. Furthermore, VV-hTAP1,2 increases splenic TAP transport activity and endogenous antigen processing, thus rendering infected targets more susceptible to CTL recognition and subsequent killing. This is the first demonstration that over-expression of a component of the antigen-processing machinery increases endogenous antigen presentation and dendritic cell cross-presentation of exogenous antigens and may provide a novel and general approach for increasing immune responses against pathogens at low doses of vaccine inocula.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Zinkernagel, Rm" uniqKey="Zinkernagel R">RM Zinkernagel</name></author><author><name sortKey="Doherty, Pc" uniqKey="Doherty P">PC Doherty</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Townsend, Ar" uniqKey="Townsend A">AR Townsend</name></author><author><name sortKey="Rothbard, J" uniqKey="Rothbard J">J Rothbard</name></author><author><name sortKey="Gotch, Fm" uniqKey="Gotch F">FM Gotch</name></author><author><name sortKey="Bahadur, G" uniqKey="Bahadur G">G Bahadur</name></author><author><name sortKey="Wraith, D" uniqKey="Wraith D">D Wraith</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Suh, Wk" uniqKey="Suh W">WK Suh</name></author><author><name sortKey="Cohen Doyle, Mf" uniqKey="Cohen Doyle M">MF Cohen-Doyle</name></author><author><name sortKey="Fruh, K" uniqKey="Fruh K">K Fruh</name></author><author><name sortKey="Wang, K" uniqKey="Wang K">K Wang</name></author><author><name sortKey="Peterson, Pa" uniqKey="Peterson P">PA Peterson</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Frisan, T" uniqKey="Frisan T">T Frisan</name></author><author><name sortKey="Zhang, Qj" uniqKey="Zhang Q">QJ Zhang</name></author><author><name sortKey="Levitskaya, J" uniqKey="Levitskaya J">J Levitskaya</name></author><author><name sortKey="Coram, M" uniqKey="Coram M">M Coram</name></author><author><name sortKey="Kurilla, Mg" uniqKey="Kurilla M">MG Kurilla</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Alimonti, J" uniqKey="Alimonti J">J Alimonti</name></author><author><name sortKey="Zhang, Qj" uniqKey="Zhang Q">QJ Zhang</name></author><author><name sortKey="Gabathuler, R" uniqKey="Gabathuler R">R Gabathuler</name></author><author><name sortKey="Reid, G" uniqKey="Reid G">G Reid</name></author><author><name sortKey="Chen, Ss" uniqKey="Chen S">SS Chen</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Deverson, Ev" uniqKey="Deverson E">EV Deverson</name></author><author><name sortKey="Gow, Ir" uniqKey="Gow I">IR Gow</name></author><author><name sortKey="Coadwell, Wj" uniqKey="Coadwell W">WJ Coadwell</name></author><author><name sortKey="Monaco, Jj" uniqKey="Monaco J">JJ Monaco</name></author><author><name sortKey="Butcher, Gw" uniqKey="Butcher G">GW Butcher</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kelly, A" uniqKey="Kelly A">A Kelly</name></author><author><name sortKey="Powis, Sh" uniqKey="Powis S">SH Powis</name></author><author><name sortKey="Glynne, R" uniqKey="Glynne R">R Glynne</name></author><author><name sortKey="Radley, E" uniqKey="Radley E">E Radley</name></author><author><name sortKey="Beck, S" uniqKey="Beck S">S Beck</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Norbury, Cc" uniqKey="Norbury C">CC Norbury</name></author><author><name sortKey="Princiotta, Mf" uniqKey="Princiotta M">MF Princiotta</name></author><author><name sortKey="Bacik, I" uniqKey="Bacik I">I Bacik</name></author><author><name sortKey="Brutkiewicz, Rr" uniqKey="Brutkiewicz R">RR Brutkiewicz</name></author><author><name sortKey="Wood, P" uniqKey="Wood P">P Wood</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ortmann, B" uniqKey="Ortmann B">B Ortmann</name></author><author><name sortKey="Androlewicz, Mj" uniqKey="Androlewicz M">MJ Androlewicz</name></author><author><name sortKey="Cresswell, P" uniqKey="Cresswell P">P Cresswell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Belyakov, Im" uniqKey="Belyakov I">IM Belyakov</name></author><author><name sortKey="Earl, P" uniqKey="Earl P">P Earl</name></author><author><name sortKey="Dzutsev, A" uniqKey="Dzutsev A">A Dzutsev</name></author><author><name sortKey="Kuznetsov, Va" uniqKey="Kuznetsov V">VA Kuznetsov</name></author><author><name sortKey="Lemon, M" uniqKey="Lemon M">M Lemon</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Takahashi, H" uniqKey="Takahashi H">H Takahashi</name></author><author><name sortKey="Takeshita, T" uniqKey="Takeshita T">T Takeshita</name></author><author><name sortKey="Morein, B" uniqKey="Morein B">B Morein</name></author><author><name sortKey="Putney, S" uniqKey="Putney S">S Putney</name></author><author><name sortKey="Germain, Rn" uniqKey="Germain R">RN Germain</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Salter, Rd" uniqKey="Salter R">RD Salter</name></author><author><name sortKey="Cresswell, P" uniqKey="Cresswell P">P Cresswell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Spies, T" uniqKey="Spies T">T Spies</name></author><author><name sortKey="Demars, R" uniqKey="Demars R">R DeMars</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Smith, Gl" uniqKey="Smith G">GL Smith</name></author><author><name sortKey="Symons, Ja" uniqKey="Symons J">JA Symons</name></author><author><name sortKey="Khanna, A" uniqKey="Khanna A">A Khanna</name></author><author><name sortKey="Vanderplasschen, A" uniqKey="Vanderplasschen A">A Vanderplasschen</name></author><author><name sortKey="Alcami, A" uniqKey="Alcami A">A Alcami</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Hooper, Jw" uniqKey="Hooper J">JW Hooper</name></author><author><name sortKey="Custer, Dm" uniqKey="Custer D">DM Custer</name></author><author><name sortKey="Schmaljohn, Cs" uniqKey="Schmaljohn C">CS Schmaljohn</name></author><author><name sortKey="Schmaljohn, Al" uniqKey="Schmaljohn A">AL Schmaljohn</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Brandt, Ta" uniqKey="Brandt T">TA Brandt</name></author><author><name sortKey="Jacobs, Bl" uniqKey="Jacobs B">BL Jacobs</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Smee, Df" uniqKey="Smee D">DF Smee</name></author><author><name sortKey="Bailey, Kw" uniqKey="Bailey K">KW Bailey</name></author><author><name sortKey="Sidwell, Rw" uniqKey="Sidwell R">RW Sidwell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Reading, Pc" uniqKey="Reading P">PC Reading</name></author><author><name sortKey="Smith, Gl" uniqKey="Smith G">GL Smith</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bellone, M" uniqKey="Bellone M">M Bellone</name></author><author><name sortKey="Iezzi, G" uniqKey="Iezzi G">G Iezzi</name></author><author><name sortKey="Manfredi, Aa" uniqKey="Manfredi A">AA Manfredi</name></author><author><name sortKey="Protti, Mp" uniqKey="Protti M">MP Protti</name></author><author><name sortKey="Dellabona, P" uniqKey="Dellabona P">P Dellabona</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Momburg, F" uniqKey="Momburg F">F Momburg</name></author><author><name sortKey="Neefjes, Jj" uniqKey="Neefjes J">JJ Neefjes</name></author><author><name sortKey="Hammerling, Gj" uniqKey="Hammerling G">GJ Hammerling</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Uebel, S" uniqKey="Uebel S">S Uebel</name></author><author><name sortKey="Meyer, Th" uniqKey="Meyer T">TH Meyer</name></author><author><name sortKey="Kraas, W" uniqKey="Kraas W">W Kraas</name></author><author><name sortKey="Kienle, S" uniqKey="Kienle S">S Kienle</name></author><author><name sortKey="Jung, G" uniqKey="Jung G">G Jung</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Motta, I" uniqKey="Motta I">I Motta</name></author><author><name sortKey="Lone, Yc" uniqKey="Lone Y">YC Lone</name></author><author><name sortKey="Kourilsky, P" uniqKey="Kourilsky P">P Kourilsky</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Armandola, Ea" uniqKey="Armandola E">EA Armandola</name></author><author><name sortKey="Momburg, F" uniqKey="Momburg F">F Momburg</name></author><author><name sortKey="Nijenhuis, M" uniqKey="Nijenhuis M">M Nijenhuis</name></author><author><name sortKey="Bulbuc, N" uniqKey="Bulbuc N">N Bulbuc</name></author><author><name sortKey="Fruh, K" uniqKey="Fruh K">K Fruh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Van Endert, Pm" uniqKey="Van Endert P">PM van Endert</name></author><author><name sortKey="Riganelli, D" uniqKey="Riganelli D">D Riganelli</name></author><author><name sortKey="Greco, G" uniqKey="Greco G">G Greco</name></author><author><name sortKey="Fleischhauer, K" uniqKey="Fleischhauer K">K Fleischhauer</name></author><author><name sortKey="Sidney, J" uniqKey="Sidney J">J Sidney</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Momburg, F" uniqKey="Momburg F">F Momburg</name></author><author><name sortKey="Roelse, J" uniqKey="Roelse J">J Roelse</name></author><author><name sortKey="Howard, Jc" uniqKey="Howard J">JC Howard</name></author><author><name sortKey="Butcher, Gw" uniqKey="Butcher G">GW Butcher</name></author><author><name sortKey="Hammerling, Gj" uniqKey="Hammerling G">GJ Hammerling</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Lizee, G" uniqKey="Lizee G">G Lizee</name></author><author><name sortKey="Basha, G" uniqKey="Basha G">G Basha</name></author><author><name sortKey="Tiong, J" uniqKey="Tiong J">J Tiong</name></author><author><name sortKey="Julien, Jp" uniqKey="Julien J">JP Julien</name></author><author><name sortKey="Tian, M" uniqKey="Tian M">M Tian</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Guermonprez, P" uniqKey="Guermonprez P">P Guermonprez</name></author><author><name sortKey="Saveanu, L" uniqKey="Saveanu L">L Saveanu</name></author><author><name sortKey="Kleijmeer, M" uniqKey="Kleijmeer M">M Kleijmeer</name></author><author><name sortKey="Davoust, J" uniqKey="Davoust J">J Davoust</name></author><author><name sortKey="Van Endert, P" uniqKey="Van Endert P">P Van Endert</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, L" uniqKey="Chen L">L Chen</name></author><author><name sortKey="Jondal, M" uniqKey="Jondal M">M Jondal</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Chen, L" uniqKey="Chen L">L Chen</name></author><author><name sortKey="Jondal, M" uniqKey="Jondal M">M Jondal</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Seliger, B" uniqKey="Seliger B">B Seliger</name></author><author><name sortKey="Wollscheid, U" uniqKey="Wollscheid U">U Wollscheid</name></author><author><name sortKey="Momburg, F" uniqKey="Momburg F">F Momburg</name></author><author><name sortKey="Blankenstein, T" uniqKey="Blankenstein T">T Blankenstein</name></author><author><name sortKey="Huber, C" uniqKey="Huber C">C Huber</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Reits, Ea" uniqKey="Reits E">EA Reits</name></author><author><name sortKey="Vos, Jc" uniqKey="Vos J">JC Vos</name></author><author><name sortKey="Gromme, M" uniqKey="Gromme M">M Gromme</name></author><author><name sortKey="Neefjes, J" uniqKey="Neefjes J">J Neefjes</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Bacik, I" uniqKey="Bacik I">I Bacik</name></author><author><name sortKey="Cox, Jh" uniqKey="Cox J">JH Cox</name></author><author><name sortKey="Anderson, R" uniqKey="Anderson R">R Anderson</name></author><author><name sortKey="Yewdell, Jw" uniqKey="Yewdell J">JW Yewdell</name></author><author><name sortKey="Bennink, Jr" uniqKey="Bennink J">JR Bennink</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Ackerman, Al" uniqKey="Ackerman A">AL Ackerman</name></author><author><name sortKey="Kyritsis, C" uniqKey="Kyritsis C">C Kyritsis</name></author><author><name sortKey="Tampe, R" uniqKey="Tampe R">R Tampe</name></author><author><name sortKey="Cresswell, P" uniqKey="Cresswell P">P Cresswell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Houde, M" uniqKey="Houde M">M Houde</name></author><author><name sortKey="Bertholet, S" uniqKey="Bertholet S">S Bertholet</name></author><author><name sortKey="Gagnon, E" uniqKey="Gagnon E">E Gagnon</name></author><author><name sortKey="Brunet, S" uniqKey="Brunet S">S Brunet</name></author><author><name sortKey="Goyette, G" uniqKey="Goyette G">G Goyette</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Melnick, Jl" uniqKey="Melnick J">JL Melnick</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Dertzbaugh, Mt" uniqKey="Dertzbaugh M">MT Dertzbaugh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Singh, M" uniqKey="Singh M">M Singh</name></author><author><name sortKey="O Hagan, D" uniqKey="O Hagan D">D O'Hagan</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Kahn, P" uniqKey="Kahn P">P Kahn</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Russ, G" uniqKey="Russ G">G Russ</name></author><author><name sortKey="Esquivel, F" uniqKey="Esquivel F">F Esquivel</name></author><author><name sortKey="Yewdell, Jw" uniqKey="Yewdell J">JW Yewdell</name></author><author><name sortKey="Cresswell, P" uniqKey="Cresswell P">P Cresswell</name></author><author><name sortKey="Spies, T" uniqKey="Spies T">T Spies</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Norbury, Cc" uniqKey="Norbury C">CC Norbury</name></author><author><name sortKey="Princiotta, Mf" uniqKey="Princiotta M">MF Princiotta</name></author><author><name sortKey="Bacik, I" uniqKey="Bacik I">I Bacik</name></author><author><name sortKey="Brutkiewicz, Rr" uniqKey="Brutkiewicz R">RR Brutkiewicz</name></author><author><name sortKey="Wood, P" uniqKey="Wood P">P Wood</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Androlewicz, Mj" uniqKey="Androlewicz M">MJ Androlewicz</name></author><author><name sortKey="Anderson, Ks" uniqKey="Anderson K">KS Anderson</name></author><author><name sortKey="Cresswell, P" uniqKey="Cresswell P">P Cresswell</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Heemels, Mt" uniqKey="Heemels M">MT Heemels</name></author><author><name sortKey="Schumacher, Tn" uniqKey="Schumacher T">TN Schumacher</name></author><author><name sortKey="Wonigeit, K" uniqKey="Wonigeit K">K Wonigeit</name></author><author><name sortKey="Ploegh, Hl" uniqKey="Ploegh H">HL Ploegh</name></author></analytic></biblStruct><biblStruct><analytic><author><name sortKey="Porgador, A" uniqKey="Porgador A">A Porgador</name></author><author><name sortKey="Yewdell, Jw" uniqKey="Yewdell J">JW Yewdell</name></author><author><name sortKey="Deng, Y" uniqKey="Deng Y">Y Deng</name></author><author><name sortKey="Bennink, Jr" uniqKey="Bennink J">JR Bennink</name></author><author><name sortKey="Germain, Rn" uniqKey="Germain R">RN Germain</name></author></analytic></biblStruct></listBibl></div1></back></TEI><affiliations><list><country><li>Canada</li></country></list><tree><country name="Canada"><noRegion><name sortKey="Vitalis, Timothy Z" sort="Vitalis, Timothy Z" uniqKey="Vitalis T" first="Timothy Z" last="Vitalis">Timothy Z. Vitalis</name></noRegion><name sortKey="Alimonti, Judie" sort="Alimonti, Judie" uniqKey="Alimonti J" first="Judie" last="Alimonti">Judie Alimonti</name><name sortKey="Alimonti, Judie" sort="Alimonti, Judie" uniqKey="Alimonti J" first="Judie" last="Alimonti">Judie Alimonti</name><name sortKey="Alimonti, Judie" sort="Alimonti, Judie" uniqKey="Alimonti J" first="Judie" last="Alimonti">Judie Alimonti</name><name sortKey="Basha, Genc" sort="Basha, Genc" uniqKey="Basha G" first="Genc" last="Basha">Genc Basha</name><name sortKey="Basha, Genc" sort="Basha, Genc" uniqKey="Basha G" first="Genc" last="Basha">Genc Basha</name><name sortKey="Chen, Susan S" sort="Chen, Susan S" uniqKey="Chen S" first="Susan S" last="Chen">Susan S. Chen</name><name sortKey="Chen, Susan S" sort="Chen, Susan S" uniqKey="Chen S" first="Susan S" last="Chen">Susan S. Chen</name><name sortKey="Chen, Susan S" sort="Chen, Susan S" uniqKey="Chen S" first="Susan S" last="Chen">Susan S. Chen</name><name sortKey="Choi, Kyung Bok" sort="Choi, Kyung Bok" uniqKey="Choi K" first="Kyung Bok" last="Choi">Kyung Bok Choi</name><name sortKey="Choi, Kyung Bok" sort="Choi, Kyung Bok" uniqKey="Choi K" first="Kyung Bok" last="Choi">Kyung Bok Choi</name><name sortKey="Jefferies, Wilfred A" sort="Jefferies, Wilfred A" uniqKey="Jefferies W" first="Wilfred A" last="Jefferies">Wilfred A. Jefferies</name><name sortKey="Jefferies, Wilfred A" sort="Jefferies, Wilfred A" uniqKey="Jefferies W" first="Wilfred A" last="Jefferies">Wilfred A. Jefferies</name><name sortKey="Jefferies, Wilfred A" sort="Jefferies, Wilfred A" uniqKey="Jefferies W" first="Wilfred A" last="Jefferies">Wilfred A. Jefferies</name><name sortKey="Jefferies, Wilfred A" sort="Jefferies, Wilfred A" uniqKey="Jefferies W" first="Wilfred A" last="Jefferies">Wilfred A. Jefferies</name><name sortKey="Jefferies, Wilfred A" sort="Jefferies, Wilfred A" uniqKey="Jefferies W" first="Wilfred A" last="Jefferies">Wilfred A. Jefferies</name><name sortKey="Jeffries, Andy" sort="Jeffries, Andy" uniqKey="Jeffries A" first="Andy" last="Jeffries">Andy Jeffries</name><name sortKey="Jeffries, Andy" sort="Jeffries, Andy" uniqKey="Jeffries A" first="Andy" last="Jeffries">Andy Jeffries</name><name sortKey="Moise, Alex" sort="Moise, Alex" uniqKey="Moise A" first="Alex" last="Moise">Alex Moise</name><name sortKey="Moise, Alex" sort="Moise, Alex" uniqKey="Moise A" first="Alex" last="Moise">Alex Moise</name><name sortKey="Moise, Alex" sort="Moise, Alex" uniqKey="Moise A" first="Alex" last="Moise">Alex Moise</name><name sortKey="Tian, Mei Mei" sort="Tian, Mei Mei" uniqKey="Tian M" first="Mei Mei" last="Tian">Mei Mei Tian</name><name sortKey="Tian, Mei Mei" sort="Tian, Mei Mei" uniqKey="Tian M" first="Mei Mei" last="Tian">Mei Mei Tian</name><name sortKey="Tian, Mei Mei" sort="Tian, Mei Mei" uniqKey="Tian M" first="Mei Mei" last="Tian">Mei Mei Tian</name><name sortKey="Tiong, Jacqueline" sort="Tiong, Jacqueline" uniqKey="Tiong J" first="Jacqueline" last="Tiong">Jacqueline Tiong</name><name sortKey="Tiong, Jacqueline" sort="Tiong, Jacqueline" uniqKey="Tiong J" first="Jacqueline" last="Tiong">Jacqueline Tiong</name><name sortKey="Tiong, Jacqueline" sort="Tiong, Jacqueline" uniqKey="Tiong J" first="Jacqueline" last="Tiong">Jacqueline Tiong</name><name sortKey="Vitalis, Timothy Z" sort="Vitalis, Timothy Z" uniqKey="Vitalis T" first="Timothy Z" last="Vitalis">Timothy Z. Vitalis</name><name sortKey="Waterfield, Douglas" sort="Waterfield, Douglas" uniqKey="Waterfield D" first="Douglas" last="Waterfield">Douglas Waterfield</name><name sortKey="Zhang, Qian Jin" sort="Zhang, Qian Jin" uniqKey="Zhang Q" first="Qian-Jin" last="Zhang">Qian-Jin Zhang</name><name sortKey="Zhang, Qian Jin" sort="Zhang, Qian Jin" uniqKey="Zhang Q" first="Qian-Jin" last="Zhang">Qian-Jin Zhang</name></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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