Comprehensive Antibody Epitope Mapping of the Nucleocapsid Protein of Severe Acute Respiratory Syndrome (SARS) Coronavirus: Insight into the Humoral Immunity of SARS
Identifieur interne : 001022 ( Ncbi/Checkpoint ); précédent : 001021; suivant : 001023Comprehensive Antibody Epitope Mapping of the Nucleocapsid Protein of Severe Acute Respiratory Syndrome (SARS) Coronavirus: Insight into the Humoral Immunity of SARS
Auteurs : Yunfei Liang ; Ying Wan ; Li-Wen Qiu ; Jingran Zhou ; Bing Ni ; Bo Guo ; Qiang Zou ; Liyun Zou ; Wei Zhou ; Zhengcai Jia ; Xiao-Yan Che ; Yuzhang WuSource :
- Clinical Chemistry [ 0009-9147 ] ; 2005.
Descripteurs français
- KwdFr :
- Adulte, Animaux, Anticorps monoclonaux, Cartographie épitopique, Femelle, Humains, Lignée cellulaire, Mâle, Production d'anticorps, Protéines nucléocapside (génétique), Protéines nucléocapside (métabolisme), Saccharomyces cerevisiae (génétique), Souris, Souris de lignée BALB C, Syndrome respiratoire aigu sévère (immunologie), Virus du SRAS (immunologie).
- MESH :
- génétique : Protéines nucléocapside, Saccharomyces cerevisiae.
- immunologie : Syndrome respiratoire aigu sévère, Virus du SRAS.
- métabolisme : Protéines nucléocapside.
- Adulte, Animaux, Anticorps monoclonaux, Cartographie épitopique, Femelle, Humains, Lignée cellulaire, Mâle, Production d'anticorps, Souris, Souris de lignée BALB C.
English descriptors
- KwdEn :
- Adult, Animals, Antibodies, Monoclonal, Antibody Formation, Cell Line, Chlorocebus aethiops, Epitope Mapping, Female, Humans, Male, Mice, Mice, Inbred BALB C, Nucleocapsid Proteins (genetics), Nucleocapsid Proteins (metabolism), SARS Virus (immunology), Saccharomyces cerevisiae (genetics), Severe Acute Respiratory Syndrome (immunology).
- MESH :
- chemical , genetics : Nucleocapsid Proteins.
- chemical , metabolism : Nucleocapsid Proteins.
- chemical : Antibodies, Monoclonal.
- genetics : Saccharomyces cerevisiae.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome.
- Adult, Animals, Antibody Formation, Cell Line, Chlorocebus aethiops, Epitope Mapping, Female, Humans, Male, Mice, Mice, Inbred BALB C.
Abstract
Url:
DOI: 10.1373/clinchem.2005.051045
PubMed: 15976093
PubMed Central: 7108199
Affiliations:
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PMC:7108199Le document en format XML
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sort="Che, Xiao Yan" uniqKey="Che X" first="Xiao-Yan" last="Che">Xiao-Yan Che</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Wu, Yuzhang" sort="Wu, Yuzhang" uniqKey="Wu Y" first="Yuzhang" last="Wu">Yuzhang Wu</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author></titleStmt><publicationStmt><idno type="wicri:source">PMC</idno><idno type="pmid">15976093</idno><idno type="pmc">7108199</idno><idno type="url">http://www.ncbi.nlm.nih.gov/pmc/articles/PMC7108199</idno><idno type="RBID">PMC:7108199</idno><idno type="doi">10.1373/clinchem.2005.051045</idno><date when="2005">2005</date><idno type="wicri:Area/Pmc/Corpus">000E90</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Corpus" wicri:corpus="PMC">000E90</idno><idno type="wicri:Area/Pmc/Curation">000E90</idno><idno type="wicri:explorRef" wicri:stream="Pmc" wicri:step="Curation">000E90</idno><idno type="wicri:Area/Pmc/Checkpoint">001368</idno><idno type="wicri:explorRef" 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SARS</title><author><name sortKey="Liang, Yunfei" sort="Liang, Yunfei" uniqKey="Liang Y" first="Yunfei" last="Liang">Yunfei Liang</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Wan, Ying" sort="Wan, Ying" uniqKey="Wan Y" first="Ying" last="Wan">Ying Wan</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Qiu, Li Wen" sort="Qiu, Li Wen" uniqKey="Qiu L" first="Li-Wen" last="Qiu">Li-Wen Qiu</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Zhou, Jingran" sort="Zhou, Jingran" uniqKey="Zhou J" first="Jingran" last="Zhou">Jingran Zhou</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Ni, Bing" sort="Ni, Bing" uniqKey="Ni B" first="Bing" last="Ni">Bing Ni</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Guo, Bo" sort="Guo, Bo" uniqKey="Guo B" first="Bo" last="Guo">Bo Guo</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Zou, Qiang" sort="Zou, Qiang" uniqKey="Zou Q" first="Qiang" last="Zou">Qiang Zou</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Zou, Liyun" sort="Zou, Liyun" uniqKey="Zou L" first="Liyun" last="Zou">Liyun Zou</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Zhou, Wei" sort="Zhou, Wei" uniqKey="Zhou W" first="Wei" last="Zhou">Wei Zhou</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Jia, Zhengcai" sort="Jia, Zhengcai" uniqKey="Jia Z" first="Zhengcai" last="Jia">Zhengcai Jia</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Che, Xiao Yan" sort="Che, Xiao Yan" uniqKey="Che X" first="Xiao-Yan" last="Che">Xiao-Yan Che</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author><author><name sortKey="Wu, Yuzhang" sort="Wu, Yuzhang" uniqKey="Wu Y" first="Yuzhang" last="Wu">Yuzhang Wu</name><affiliation><nlm:aff>NONE</nlm:aff></affiliation></author></analytic><series><title level="j">Clinical Chemistry</title><idno type="ISSN">0009-9147</idno><idno type="eISSN">1530-8561</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adult</term><term>Animals</term><term>Antibodies, Monoclonal</term><term>Antibody Formation</term><term>Cell Line</term><term>Chlorocebus aethiops</term><term>Epitope Mapping</term><term>Female</term><term>Humans</term><term>Male</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Nucleocapsid Proteins (genetics)</term><term>Nucleocapsid Proteins (metabolism)</term><term>SARS Virus (immunology)</term><term>Saccharomyces cerevisiae (genetics)</term><term>Severe Acute Respiratory Syndrome (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adulte</term><term>Animaux</term><term>Anticorps monoclonaux</term><term>Cartographie épitopique</term><term>Femelle</term><term>Humains</term><term>Lignée cellulaire</term><term>Mâle</term><term>Production d'anticorps</term><term>Protéines nucléocapside (génétique)</term><term>Protéines nucléocapside (métabolisme)</term><term>Saccharomyces cerevisiae (génétique)</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Nucleocapsid Proteins</term></keywords><keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Nucleocapsid Proteins</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Antibodies, Monoclonal</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Protéines nucléocapside</term><term>Saccharomyces cerevisiae</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines nucléocapside</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adult</term><term>Animals</term><term>Antibody Formation</term><term>Cell Line</term><term>Chlorocebus aethiops</term><term>Epitope Mapping</term><term>Female</term><term>Humans</term><term>Male</term><term>Mice</term><term>Mice, Inbred BALB C</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adulte</term><term>Animaux</term><term>Anticorps monoclonaux</term><term>Cartographie épitopique</term><term>Femelle</term><term>Humains</term><term>Lignée cellulaire</term><term>Mâle</term><term>Production d'anticorps</term><term>Souris</term><term>Souris de lignée BALB C</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><title>Abstract</title><p><italic>Background:</italic> The epidemic outbreak of severe acute respiratory syndrome (SARS) posed a worldwide threat to public health and economic stability. Although the pandemic has been contained, concerns over its recurrence remain. It is essential to identify specific diagnostic agents and antiviral vaccine candidates to fight this highly contagious disease.</p><p><italic>Methods:</italic> We generated 14 monoclonal antibodies (mAbs) specific to the SARS coronavirus (SARS-CoV) nucleocapsid (N) protein and used these to thoroughly map the N protein antigenic determinants. We identified the immunodominant antigenic sites responsible for the antibodies in sera from SARS patients and antisera from small animals and differentiated the linear from the conformational antibody-combining sites comprising the natural epitopes by use of yeast surface display.</p><p><italic>Results:</italic> We identified 5 conformational and 3 linear epitopes within the entire N protein; 3 conformational and 3 linear epitopes were immunodominant. The antibody responses to the N protein fragments in mammalian sera revealed that 3 regions of the N protein are strong antigenic domains. We expanded the specificity of the N protein epitope and identified 4 novel conformational epitopes (amino acids 1–69, 68–213, 212–341, and 337–422).</p><p><italic>Conclusion:</italic> The antigenic structures identified for the SARS-CoV N protein, the epitope-specific mAbs, and the serum antibody profile in SARS patients have potential use in the clinical diagnosis and understanding of the protective immunity to SARS-CoV.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct><biblStruct></biblStruct></listBibl></div1></back></TEI><affiliations><list></list><tree><noCountry><name sortKey="Che, Xiao Yan" sort="Che, Xiao Yan" uniqKey="Che X" first="Xiao-Yan" last="Che">Xiao-Yan Che</name><name sortKey="Guo, Bo" sort="Guo, Bo" uniqKey="Guo B" first="Bo" last="Guo">Bo Guo</name><name sortKey="Jia, Zhengcai" sort="Jia, Zhengcai" uniqKey="Jia Z" first="Zhengcai" last="Jia">Zhengcai Jia</name><name sortKey="Liang, Yunfei" sort="Liang, Yunfei" uniqKey="Liang Y" first="Yunfei" last="Liang">Yunfei Liang</name><name sortKey="Ni, Bing" sort="Ni, Bing" uniqKey="Ni B" first="Bing" last="Ni">Bing Ni</name><name sortKey="Qiu, Li Wen" sort="Qiu, Li Wen" uniqKey="Qiu L" first="Li-Wen" last="Qiu">Li-Wen Qiu</name><name sortKey="Wan, Ying" sort="Wan, Ying" uniqKey="Wan Y" first="Ying" last="Wan">Ying Wan</name><name sortKey="Wu, Yuzhang" sort="Wu, Yuzhang" uniqKey="Wu Y" first="Yuzhang" last="Wu">Yuzhang Wu</name><name sortKey="Zhou, Jingran" sort="Zhou, Jingran" uniqKey="Zhou J" first="Jingran" last="Zhou">Jingran Zhou</name><name sortKey="Zhou, Wei" sort="Zhou, Wei" uniqKey="Zhou W" first="Wei" last="Zhou">Wei Zhou</name><name sortKey="Zou, Liyun" sort="Zou, Liyun" uniqKey="Zou L" first="Liyun" last="Zou">Liyun Zou</name><name sortKey="Zou, Qiang" sort="Zou, Qiang" uniqKey="Zou Q" first="Qiang" last="Zou">Qiang Zou</name></noCountry></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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