Multiplexed detection of pathogen DNA with DNA-based fluorescence nanobarcodes.
Identifieur interne : 001007 ( Ncbi/Checkpoint ); précédent : 001006; suivant : 001008Multiplexed detection of pathogen DNA with DNA-based fluorescence nanobarcodes.
Auteurs : Yougen Li [États-Unis] ; Yen Thi Hong Cu ; Dan LuoSource :
- Nature biotechnology [ 1087-0156 ] ; 2005.
Descripteurs français
- KwdFr :
- ADN bactérien (analyse), ADN viral (analyse), Bacillus anthracis (génétique), Bacillus anthracis (isolement et purification), Colorants fluorescents (), Colorants fluorescents (analyse), Cytométrie en flux, Données de séquences moléculaires, Fluorescence, Francisella tularensis (génétique), Francisella tularensis (isolement et purification), Hybridation d'acides nucléiques (), Nanostructures (), Nanotechnologie, Sondes d'ADN (), Sondes d'ADN (analyse), Sondes d'ADN (synthèse chimique), Séquence nucléotidique, Virus du SRAS (génétique), Virus du SRAS (isolement et purification).
- MESH :
- analyse : ADN bactérien, ADN viral, Colorants fluorescents, Sondes d'ADN.
- génétique : Bacillus anthracis, Francisella tularensis, Virus du SRAS.
- isolement et purification : Bacillus anthracis, Francisella tularensis, Virus du SRAS.
- synthèse chimique : Sondes d'ADN.
- Colorants fluorescents, Cytométrie en flux, Données de séquences moléculaires, Fluorescence, Hybridation d'acides nucléiques, Nanostructures, Nanotechnologie, Sondes d'ADN, Séquence nucléotidique.
English descriptors
- KwdEn :
- Bacillus anthracis (genetics), Bacillus anthracis (isolation & purification), Base Sequence, DNA Probes (analysis), DNA Probes (chemical synthesis), DNA Probes (chemistry), DNA, Bacterial (analysis), DNA, Viral (analysis), Flow Cytometry, Fluorescence, Fluorescent Dyes (analysis), Fluorescent Dyes (chemistry), Francisella tularensis (genetics), Francisella tularensis (isolation & purification), Molecular Sequence Data, Nanostructures (chemistry), Nanotechnology, Nucleic Acid Hybridization (methods), SARS Virus (genetics), SARS Virus (isolation & purification).
- MESH :
- chemical , analysis : DNA Probes, DNA, Bacterial, DNA, Viral, Fluorescent Dyes.
- chemical , chemical synthesis : DNA Probes.
- chemical , chemistry : DNA Probes, Fluorescent Dyes.
- chemistry : Nanostructures.
- genetics : Bacillus anthracis, Francisella tularensis, SARS Virus.
- isolation & purification : Bacillus anthracis, Francisella tularensis, SARS Virus.
- methods : Nucleic Acid Hybridization.
- Base Sequence, Flow Cytometry, Fluorescence, Molecular Sequence Data, Nanotechnology.
Abstract
Rapid, multiplexed, sensitive and specific molecular detection is of great demand in gene profiling, drug screening, clinical diagnostics and environmental analysis. One of the major challenges in multiplexed analysis is to identify each specific reaction with a distinct label or 'code'. Two encoding strategies are currently used: positional encoding, in which every potential reaction is preassigned a particular position on a solid-phase support such as a DNA microarray, and reaction encoding, where every possible reaction is uniquely tagged with a code that is most often optical or particle based. The micrometer size, polydispersity, complex fabrication process and nonbiocompatibility of current codes limit their usability. Here we demonstrate the synthesis of dendrimer-like DNA-based, fluorescence-intensity-coded nanobarcodes, which contain a built-in code and a probe for molecular recognition. Their application to multiplexed detection of the DNA of several pathogens is first shown using fluorescence microscopy and dot blotting, and further demonstrated using flow cytometry that resulted in detection that was sensitive (attomole) and rapid.
DOI: 10.1038/nbt1106
PubMed: 15951805
Affiliations:
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pubmed:15951805Le document en format XML
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