Immunofluorescence assay for detection of the nucleocapsid antigen of the severe acute respiratory syndrome (SARS)-associated coronavirus in cells derived from throat wash samples of patients with SARS.
Identifieur interne : 000F31 ( Ncbi/Checkpoint ); précédent : 000F30; suivant : 000F32Immunofluorescence assay for detection of the nucleocapsid antigen of the severe acute respiratory syndrome (SARS)-associated coronavirus in cells derived from throat wash samples of patients with SARS.
Auteurs : I-Jung Liu [Taïwan] ; Pei-Jer Chen ; Shiou-Hwei Yeh ; Yu-Ping Chiang ; Li-Min Huang ; Ming-Fu Chang ; Shey-Ying Chen ; Pan-Chyr Yang ; Shan-Chwen Chang ; Wei-Kung WangSource :
- Journal of clinical microbiology [ 0095-1137 ] ; 2005.
Descripteurs français
- KwdFr :
- Antigènes viraux (analyse), Humains, Nucléocapside (analyse), Pharynx (anatomopathologie), Pharynx (virologie), Syndrome respiratoire aigu sévère (anatomopathologie), Syndrome respiratoire aigu sévère (diagnostic), Technique d'immunofluorescence, Virus du SRAS (immunologie), Virus du SRAS (isolement et purification).
- MESH :
- analyse : Antigènes viraux, Nucléocapside.
- anatomopathologie : Pharynx, Syndrome respiratoire aigu sévère.
- diagnostic : Syndrome respiratoire aigu sévère.
- immunologie : Virus du SRAS.
- isolement et purification : Virus du SRAS.
- virologie : Pharynx.
- Humains, Technique d'immunofluorescence.
English descriptors
- KwdEn :
- MESH :
- chemical , analysis : Antigens, Viral.
- analysis : Nucleocapsid.
- diagnosis : Severe Acute Respiratory Syndrome.
- immunology : SARS Virus.
- isolation & purification : SARS Virus.
- pathology : Pharynx, Severe Acute Respiratory Syndrome.
- virology : Pharynx.
- Fluorescent Antibody Technique, Humans.
Abstract
An antigen detection assay for severe acute respiratory syndrome (SARS) coronavirus was established in this study by an indirect immunofluorescence test, which utilized cells derived from throat wash samples of patients with SARS and a rabbit serum that recognized the nucleocapsid protein of SARS-associated coronavirus (SARS-CoV) but not that of other human coronavirus tested. It detected SARS-CoV in 11 of 17 (65%) samples from SARS patients as early as day 2 of illness but in none of the 10 samples from healthy controls. Compared with other diagnostic modalities for detecting SARS-CoV, this assay is simpler, more convenient, and economical. It could be an alternative for early and rapid diagnosis, should SARS return in the future.
DOI: 10.1128/JCM.43.5.2444-2448.2005
PubMed: 15872279
Affiliations:
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pubmed:15872279Le document en format XML
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SRAS (isolement et purification)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Antigens, Viral</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Antigènes viraux</term><term>Nucléocapside</term></keywords><keywords scheme="MESH" qualifier="analysis" xml:lang="en"><term>Nucleocapsid</term></keywords><keywords scheme="MESH" qualifier="anatomopathologie" xml:lang="fr"><term>Pharynx</term><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="diagnosis" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" qualifier="diagnostic" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" 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derived from throat wash samples of patients with SARS and a rabbit serum that recognized the nucleocapsid protein of SARS-associated coronavirus (SARS-CoV) but not that of other human coronavirus tested. It detected SARS-CoV in 11 of 17 (65%) samples from SARS patients as early as day 2 of illness but in none of the 10 samples from healthy controls. Compared with other diagnostic modalities for detecting SARS-CoV, this assay is simpler, more convenient, and economical. It could be an alternative for early and rapid diagnosis, should SARS return in the future.</div></front></TEI><affiliations><list><country><li>Taïwan</li></country></list><tree><noCountry><name sortKey="Chang, Ming Fu" sort="Chang, Ming Fu" uniqKey="Chang M" first="Ming-Fu" last="Chang">Ming-Fu Chang</name><name sortKey="Chang, Shan Chwen" sort="Chang, Shan Chwen" uniqKey="Chang S" first="Shan-Chwen" last="Chang">Shan-Chwen Chang</name><name sortKey="Chen, Pei Jer" sort="Chen, Pei Jer" uniqKey="Chen P" first="Pei-Jer" last="Chen">Pei-Jer Chen</name><name sortKey="Chen, Shey Ying" sort="Chen, Shey Ying" uniqKey="Chen S" first="Shey-Ying" last="Chen">Shey-Ying Chen</name><name sortKey="Chiang, Yu Ping" sort="Chiang, Yu Ping" uniqKey="Chiang Y" first="Yu-Ping" last="Chiang">Yu-Ping Chiang</name><name sortKey="Huang, Li Min" sort="Huang, Li Min" uniqKey="Huang L" first="Li-Min" last="Huang">Li-Min Huang</name><name sortKey="Wang, Wei Kung" sort="Wang, Wei Kung" uniqKey="Wang W" first="Wei-Kung" last="Wang">Wei-Kung Wang</name><name sortKey="Yang, Pan Chyr" sort="Yang, Pan Chyr" uniqKey="Yang P" first="Pan-Chyr" last="Yang">Pan-Chyr Yang</name><name sortKey="Yeh, Shiou Hwei" sort="Yeh, Shiou Hwei" uniqKey="Yeh S" first="Shiou-Hwei" last="Yeh">Shiou-Hwei Yeh</name></noCountry><country name="Taïwan"><noRegion><name sortKey="Liu, I Jung" sort="Liu, I Jung" uniqKey="Liu I" first="I-Jung" last="Liu">I-Jung Liu</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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