Immune responses in Balb/c mice induced by a candidate SARS-CoV inactivated vaccine prepared from F69 strain
Identifieur interne : 000F02 ( Ncbi/Checkpoint ); précédent : 000F01; suivant : 000F03Immune responses in Balb/c mice induced by a candidate SARS-CoV inactivated vaccine prepared from F69 strain
Auteurs : Chuan-Hai Zhang [République populaire de Chine] ; Jia-Hai Lu [République populaire de Chine] ; Yi-Fei Wang [République populaire de Chine] ; Huan-Ying Zheng [République populaire de Chine] ; Sheng Xiong [République populaire de Chine] ; Mei-Ying Zhang [République populaire de Chine] ; Xin-Jian Liu [République populaire de Chine] ; Jiu-Xiang Li [République populaire de Chine] ; Zhuo-Yue Wan [République populaire de Chine] ; Xin-Ge Yan [République populaire de Chine] ; Shu-Yuan Qi [République populaire de Chine] ; Zhiyong Cui [États-Unis] ; Biliang Zhang [République populaire de Chine, États-Unis]Source :
- Vaccine [ 0264-410X ] ; 2005.
Descripteurs français
- KwdFr :
- Animaux, Anticorps antiviraux (analyse), Anticorps antiviraux (biosynthèse), Données de séquences moléculaires, Immunisation, Immunoglobuline G (analyse), Immunoglobuline G (biosynthèse), Immunoglobuline M (analyse), Immunoglobuline M (biosynthèse), Souris, Souris de lignée BALB C, Spécificité des anticorps, Syndrome respiratoire aigu sévère (immunologie), Syndrome respiratoire aigu sévère (virologie), Technique de Western, Test ELISA, Tests de neutralisation, Vaccins antiviraux (immunologie), Vaccins inactivés, Virus du SRAS (immunologie).
- MESH :
- analyse : Anticorps antiviraux, Immunoglobuline G, Immunoglobuline M.
- biosynthèse : Anticorps antiviraux, Immunoglobuline G, Immunoglobuline M.
- immunologie : Syndrome respiratoire aigu sévère, Vaccins antiviraux, Virus du SRAS.
- virologie : Syndrome respiratoire aigu sévère.
- Animaux, Données de séquences moléculaires, Immunisation, Souris, Souris de lignée BALB C, Spécificité des anticorps, Technique de Western, Test ELISA, Tests de neutralisation, Vaccins inactivés.
English descriptors
- KwdEn :
- Animals, Antibodies, Viral (analysis), Antibodies, Viral (biosynthesis), Antibody Specificity, Blotting, Western, Enzyme-Linked Immunosorbent Assay, Immunization, Immunoglobulin G (analysis), Immunoglobulin G (biosynthesis), Immunoglobulin M (analysis), Immunoglobulin M (biosynthesis), Mice, Mice, Inbred BALB C, Molecular Sequence Data, Neutralization Tests, SARS Virus (immunology), Severe Acute Respiratory Syndrome (immunology), Severe Acute Respiratory Syndrome (virology), Vaccines, Inactivated, Viral Vaccines (immunology).
- MESH :
- chemical , analysis : Antibodies, Viral, Immunoglobulin G, Immunoglobulin M.
- chemical , biosynthesis : Antibodies, Viral, Immunoglobulin G, Immunoglobulin M.
- immunology : SARS Virus, Severe Acute Respiratory Syndrome, Viral Vaccines.
- virology : Severe Acute Respiratory Syndrome.
- Animals, Antibody Specificity, Blotting, Western, Enzyme-Linked Immunosorbent Assay, Immunization, Mice, Mice, Inbred BALB C, Molecular Sequence Data, Neutralization Tests, Vaccines, Inactivated.
Abstract
The immunogenicity of a candidate-inactivated vaccine prepared from SARS-CoV F69 strain was evaluated in Balb/c mice. Potent humoral immune responses were induced under the elicitation of three times of immunizations at 2-week intervals with this vaccine, combined with three types of adjuvants (Freund's adjuvant, Al(OH)3 adjuvant and CpG adjuvant). Titers of specific IgG antibodies in three test groups all peaked in the sixth week after first vaccination, but significant differences existed in the kinetics of specific IgG antibody levels. The strong neutralizing capacity exhibited in micro-cytopathic effect neutralization tests indicated the specific antibodies are protective. Western blot assay further demonstrated the specificity of the induced serum antibodies.
Url:
DOI: 10.1016/j.vaccine.2004.11.073
PubMed: 15837220
PubMed Central: 7115646
Affiliations:
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Zhang</name><affiliation wicri:level="1"><nlm:aff id="aff1">Biomedicine Research and Development Center, Jinan University, Guangzhou 510632, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Biomedicine Research and Development Center, Jinan University, Guangzhou 510632</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation><affiliation wicri:level="1"><nlm:aff id="aff2">West Anhui University, Lu-an 237012, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>West Anhui University, Lu-an 237012</wicri:regionArea><wicri:noRegion>Lu-an 237012</wicri:noRegion></affiliation><affiliation wicri:level="1"><nlm:aff id="aff6">South China Institute of Botany, Graduate School of the Chinese Academy of Sciences, Guangzhou 510650, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>South China Institute of Botany, Graduate School of the Chinese Academy of Sciences, Guangzhou 510650</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Lu, Jia Hai" sort="Lu, Jia Hai" uniqKey="Lu J" first="Jia-Hai" last="Lu">Jia-Hai Lu</name><affiliation wicri:level="1"><nlm:aff id="aff3">School of Public Health, Sun Yat-sen University, Guangzhou 510080, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>School of Public Health, Sun Yat-sen University, Guangzhou 510080</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Wang, Yi Fei" sort="Wang, Yi Fei" uniqKey="Wang Y" first="Yi-Fei" last="Wang">Yi-Fei Wang</name><affiliation wicri:level="1"><nlm:aff id="aff1">Biomedicine Research and Development Center, Jinan University, Guangzhou 510632, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Biomedicine Research and Development Center, Jinan University, Guangzhou 510632</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Zheng, Huan Ying" sort="Zheng, Huan Ying" uniqKey="Zheng H" first="Huan-Ying" last="Zheng">Huan-Ying Zheng</name><affiliation wicri:level="1"><nlm:aff id="aff5">Center for Diseases Control and Prevention of Guangdong Province, Guangzhou 510300, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Center for Diseases Control and Prevention of Guangdong Province, Guangzhou 510300</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Xiong, Sheng" sort="Xiong, Sheng" uniqKey="Xiong S" first="Sheng" last="Xiong">Sheng Xiong</name><affiliation wicri:level="1"><nlm:aff id="aff1">Biomedicine Research and Development Center, Jinan University, Guangzhou 510632, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Biomedicine Research and Development Center, Jinan University, Guangzhou 510632</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Zhang, Mei Ying" sort="Zhang, Mei Ying" uniqKey="Zhang M" first="Mei-Ying" last="Zhang">Mei-Ying Zhang</name><affiliation wicri:level="1"><nlm:aff id="aff1">Biomedicine Research and Development Center, Jinan University, Guangzhou 510632, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Biomedicine Research and Development Center, Jinan University, Guangzhou 510632</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Liu, Xin Jian" sort="Liu, Xin Jian" uniqKey="Liu X" first="Xin-Jian" last="Liu">Xin-Jian Liu</name><affiliation wicri:level="1"><nlm:aff id="aff1">Biomedicine Research and Development Center, Jinan University, Guangzhou 510632, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Biomedicine Research and Development Center, Jinan University, Guangzhou 510632</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Li, Jiu Xiang" sort="Li, Jiu Xiang" uniqKey="Li J" first="Jiu-Xiang" last="Li">Jiu-Xiang Li</name><affiliation wicri:level="1"><nlm:aff id="aff1">Biomedicine Research and Development Center, Jinan University, Guangzhou 510632, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Biomedicine Research and Development Center, Jinan University, Guangzhou 510632</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Wan, Zhuo Yue" sort="Wan, Zhuo Yue" uniqKey="Wan Z" first="Zhuo-Yue" last="Wan">Zhuo-Yue Wan</name><affiliation wicri:level="1"><nlm:aff id="aff5">Center for Diseases Control and Prevention of Guangdong Province, Guangzhou 510300, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Center for Diseases Control and Prevention of Guangdong Province, Guangzhou 510300</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Yan, Xin Ge" sort="Yan, Xin Ge" uniqKey="Yan X" first="Xin-Ge" last="Yan">Xin-Ge Yan</name><affiliation wicri:level="1"><nlm:aff id="aff5">Center for Diseases Control and Prevention of Guangdong Province, Guangzhou 510300, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Center for Diseases Control and Prevention of Guangdong Province, Guangzhou 510300</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Qi, Shu Yuan" sort="Qi, Shu Yuan" uniqKey="Qi S" first="Shu-Yuan" last="Qi">Shu-Yuan Qi</name><affiliation wicri:level="1"><nlm:aff id="aff6">South China Institute of Botany, Graduate School of the Chinese Academy of Sciences, Guangzhou 510650, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>South China Institute of Botany, Graduate School of the Chinese Academy of Sciences, Guangzhou 510650</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation></author><author><name sortKey="Cui, Zhiyong" sort="Cui, Zhiyong" uniqKey="Cui Z" first="Zhiyong" last="Cui">Zhiyong Cui</name><affiliation wicri:level="2"><nlm:aff id="aff7">Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605</wicri:regionArea><placeName><region type="state">Massachusetts</region></placeName></affiliation></author><author><name sortKey="Zhang, Biliang" sort="Zhang, Biliang" uniqKey="Zhang B" first="Biliang" last="Zhang">Biliang Zhang</name><affiliation wicri:level="1"><nlm:aff id="aff4">Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou International Business Incubator, Guangzhou Science Park, Guangzhou 510663, China</nlm:aff><country xml:lang="fr">République populaire de Chine</country><wicri:regionArea>Guangzhou Institute of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou International Business Incubator, Guangzhou Science Park, Guangzhou 510663</wicri:regionArea><placeName><settlement type="city">Jiangmen</settlement><region type="province">Guangdong</region></placeName></affiliation><affiliation wicri:level="2"><nlm:aff id="aff7">Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605, USA</nlm:aff><country xml:lang="fr">États-Unis</country><wicri:regionArea>Program in Molecular Medicine, University of Massachusetts Medical School, Worcester, MA 01605</wicri:regionArea><placeName><region type="state">Massachusetts</region></placeName></affiliation></author></analytic><series><title level="j">Vaccine</title><idno type="ISSN">0264-410X</idno><idno type="eISSN">1873-2518</idno><imprint><date when="2005">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Antibodies, Viral (analysis)</term><term>Antibodies, Viral (biosynthesis)</term><term>Antibody Specificity</term><term>Blotting, Western</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Immunization</term><term>Immunoglobulin G (analysis)</term><term>Immunoglobulin G (biosynthesis)</term><term>Immunoglobulin M (analysis)</term><term>Immunoglobulin M (biosynthesis)</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Molecular Sequence Data</term><term>Neutralization Tests</term><term>SARS Virus (immunology)</term><term>Severe Acute Respiratory Syndrome (immunology)</term><term>Severe Acute Respiratory Syndrome (virology)</term><term>Vaccines, Inactivated</term><term>Viral Vaccines (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Anticorps antiviraux (analyse)</term><term>Anticorps antiviraux (biosynthèse)</term><term>Données de séquences moléculaires</term><term>Immunisation</term><term>Immunoglobuline G (analyse)</term><term>Immunoglobuline G (biosynthèse)</term><term>Immunoglobuline M (analyse)</term><term>Immunoglobuline M (biosynthèse)</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Spécificité des anticorps</term><term>Syndrome respiratoire aigu sévère (immunologie)</term><term>Syndrome respiratoire aigu sévère (virologie)</term><term>Technique de Western</term><term>Test ELISA</term><term>Tests de neutralisation</term><term>Vaccins antiviraux (immunologie)</term><term>Vaccins inactivés</term><term>Virus du SRAS (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="analysis" xml:lang="en"><term>Antibodies, Viral</term><term>Immunoglobulin G</term><term>Immunoglobulin M</term></keywords><keywords scheme="MESH" type="chemical" qualifier="biosynthesis" xml:lang="en"><term>Antibodies, Viral</term><term>Immunoglobulin G</term><term>Immunoglobulin M</term></keywords><keywords scheme="MESH" qualifier="analyse" xml:lang="fr"><term>Anticorps antiviraux</term><term>Immunoglobuline G</term><term>Immunoglobuline M</term></keywords><keywords scheme="MESH" qualifier="biosynthèse" xml:lang="fr"><term>Anticorps antiviraux</term><term>Immunoglobuline G</term><term>Immunoglobuline M</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term><term>Vaccins antiviraux</term><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term><term>Severe Acute Respiratory Syndrome</term><term>Viral Vaccines</term></keywords><keywords scheme="MESH" qualifier="virologie" xml:lang="fr"><term>Syndrome respiratoire aigu sévère</term></keywords><keywords scheme="MESH" qualifier="virology" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Antibody Specificity</term><term>Blotting, Western</term><term>Enzyme-Linked Immunosorbent Assay</term><term>Immunization</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Molecular Sequence Data</term><term>Neutralization Tests</term><term>Vaccines, Inactivated</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Données de séquences moléculaires</term><term>Immunisation</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Spécificité des anticorps</term><term>Technique de Western</term><term>Test ELISA</term><term>Tests de neutralisation</term><term>Vaccins inactivés</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en"><p>The immunogenicity of a candidate-inactivated vaccine prepared from SARS-CoV F69 strain was evaluated in Balb/c mice. Potent humoral immune responses were induced under the elicitation of three times of immunizations at 2-week intervals with this vaccine, combined with three types of adjuvants (Freund's adjuvant, Al(OH)<sub>3</sub> adjuvant and CpG adjuvant). Titers of specific IgG antibodies in three test groups all peaked in the sixth week after first vaccination, but significant differences existed in the kinetics of specific IgG antibody levels. The strong neutralizing capacity exhibited in micro-cytopathic effect neutralization tests indicated the specific antibodies are protective. Western blot assay further demonstrated the specificity of the induced serum antibodies.</p></div></front><back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Zhang, C H" uniqKey="Zhang C">C.H. Zhang</name></author><author><name sortKey="Wang, Y F" uniqKey="Wang Y">Y.F. Wang</name></author><author><name sortKey="Zhang, M Y" uniqKey="Zhang M">M.Y. 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