Intranasal immunization with inactivated SARS-CoV (SARS-associated coronavirus) induced local and serum antibodies in mice.
Identifieur interne : 000C60 ( Ncbi/Checkpoint ); précédent : 000C59; suivant : 000C61Intranasal immunization with inactivated SARS-CoV (SARS-associated coronavirus) induced local and serum antibodies in mice.
Auteurs : Di Qu [République populaire de Chine] ; Bojian Zheng ; Xin Yao ; Yi Guan ; Zheng-Hong Yuan ; Nan-Shan Zhong ; Li-Wei Lu ; Jian-Ping Xie ; Yu-Mei WenSource :
- Vaccine [ 0264-410X ] ; 2005.
Descripteurs français
- KwdFr :
- Administration par voie nasale, Animaux, Anticorps antiviraux (biosynthèse), Anticorps antiviraux (sang), Cellules Vero, Femelle, Humains, Mâle, Poumon (immunologie), Poumon (métabolisme), Souris, Souris de lignée BALB C, Vaccins antiviraux (administration et posologie), Vaccins antiviraux (immunologie), Vaccins inactivés (administration et posologie), Vaccins inactivés (immunologie), Virus du SRAS (immunologie).
- MESH :
- administration et posologie : Vaccins antiviraux, Vaccins inactivés.
- biosynthèse : Anticorps antiviraux.
- immunologie : Poumon, Vaccins antiviraux, Vaccins inactivés, Virus du SRAS.
- métabolisme : Poumon.
- sang : Anticorps antiviraux.
- Administration par voie nasale, Animaux, Cellules Vero, Femelle, Humains, Mâle, Souris, Souris de lignée BALB C.
English descriptors
- KwdEn :
- Administration, Intranasal, Animals, Antibodies, Viral (biosynthesis), Antibodies, Viral (blood), Chlorocebus aethiops, Female, Humans, Lung (immunology), Lung (metabolism), Male, Mice, Mice, Inbred BALB C, SARS Virus (immunology), Vaccines, Inactivated (administration & dosage), Vaccines, Inactivated (immunology), Vero Cells, Viral Vaccines (administration & dosage), Viral Vaccines (immunology).
- MESH :
- chemical , administration & dosage : Vaccines, Inactivated, Viral Vaccines.
- chemical , biosynthesis : Antibodies, Viral.
- chemical , blood : Antibodies, Viral.
- immunology : Lung, SARS Virus, Vaccines, Inactivated, Viral Vaccines.
- metabolism : Lung.
- Administration, Intranasal, Animals, Chlorocebus aethiops, Female, Humans, Male, Mice, Mice, Inbred BALB C, Vero Cells.
Abstract
SARS-CoV (severe acute respiratory syndrome-associated coronavirus) strain GZ50 was partially purified and inactivated with 1:2000 formaldehyde. In cell culture the inactivated virus blocked the replication of live virus by decreasing the TCID(5.0) of the live virus 10(3.6) to 10(4.6) times. Inactivated GZ50 was used to immunize mice intranasally either alone, or after precipitation with polyethylene glycol (PEG), or with CpG, or CTB as an adjuvant. The titer of serum neutralizing antibodies was up to 1:640. In mice immunized with adjuvants or PEG precipitated GZ50, specific IgA was detected in tracheal-lung wash fluid by immunofluorescence. Though serum antibodies were detected, no anti-SARS-IgA could be detected in mice immunized only with inactivated GZ50. The roles of adjuvants in intranasal immunization with inactivated. SARS-CoV is discussed.
DOI: 10.1016/j.vaccine.2004.07.031
PubMed: 15603894
Affiliations:
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pubmed:15603894Le document en format XML
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first="Zheng-Hong" last="Yuan">Zheng-Hong Yuan</name></author><author><name sortKey="Zhong, Nan Shan" sort="Zhong, Nan Shan" uniqKey="Zhong N" first="Nan-Shan" last="Zhong">Nan-Shan Zhong</name></author><author><name sortKey="Lu, Li Wei" sort="Lu, Li Wei" uniqKey="Lu L" first="Li-Wei" last="Lu">Li-Wei Lu</name></author><author><name sortKey="Xie, Jian Ping" sort="Xie, Jian Ping" uniqKey="Xie J" first="Jian-Ping" last="Xie">Jian-Ping Xie</name></author><author><name sortKey="Wen, Yu Mei" sort="Wen, Yu Mei" uniqKey="Wen Y" first="Yu-Mei" last="Wen">Yu-Mei Wen</name></author></analytic><series><title level="j">Vaccine</title><idno type="ISSN">0264-410X</idno><imprint><date when="2005" type="published">2005</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Administration, Intranasal</term><term>Animals</term><term>Antibodies, Viral (biosynthesis)</term><term>Antibodies, Viral (blood)</term><term>Chlorocebus 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xml:lang="en">SARS-CoV (severe acute respiratory syndrome-associated coronavirus) strain GZ50 was partially purified and inactivated with 1:2000 formaldehyde. In cell culture the inactivated virus blocked the replication of live virus by decreasing the TCID(5.0) of the live virus 10(3.6) to 10(4.6) times. Inactivated GZ50 was used to immunize mice intranasally either alone, or after precipitation with polyethylene glycol (PEG), or with CpG, or CTB as an adjuvant. The titer of serum neutralizing antibodies was up to 1:640. In mice immunized with adjuvants or PEG precipitated GZ50, specific IgA was detected in tracheal-lung wash fluid by immunofluorescence. Though serum antibodies were detected, no anti-SARS-IgA could be detected in mice immunized only with inactivated GZ50. The roles of adjuvants in intranasal immunization with inactivated. SARS-CoV is discussed.</div></front></TEI><affiliations><list><country><li>République populaire de Chine</li></country></list><tree><noCountry><name sortKey="Guan, Yi" sort="Guan, Yi" uniqKey="Guan Y" first="Yi" last="Guan">Yi Guan</name><name sortKey="Lu, Li Wei" sort="Lu, Li Wei" uniqKey="Lu L" first="Li-Wei" last="Lu">Li-Wei Lu</name><name sortKey="Wen, Yu Mei" sort="Wen, Yu Mei" uniqKey="Wen Y" first="Yu-Mei" last="Wen">Yu-Mei Wen</name><name sortKey="Xie, Jian Ping" sort="Xie, Jian Ping" uniqKey="Xie J" first="Jian-Ping" last="Xie">Jian-Ping Xie</name><name sortKey="Yao, Xin" sort="Yao, Xin" uniqKey="Yao X" first="Xin" last="Yao">Xin Yao</name><name sortKey="Yuan, Zheng Hong" sort="Yuan, Zheng Hong" uniqKey="Yuan Z" first="Zheng-Hong" last="Yuan">Zheng-Hong Yuan</name><name sortKey="Zheng, Bojian" sort="Zheng, Bojian" uniqKey="Zheng B" first="Bojian" last="Zheng">Bojian Zheng</name><name sortKey="Zhong, Nan Shan" sort="Zhong, Nan Shan" uniqKey="Zhong N" first="Nan-Shan" last="Zhong">Nan-Shan Zhong</name></noCountry><country name="République populaire de Chine"><noRegion><name sortKey="Qu, Di" sort="Qu, Di" uniqKey="Qu D" first="Di" last="Qu">Di Qu</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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