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Severe Acute Respiratory Syndrome Coronavirus on Hospital Surfaces

Identifieur interne : 000A59 ( Ncbi/Checkpoint ); précédent : 000A58; suivant : 000A60

Severe Acute Respiratory Syndrome Coronavirus on Hospital Surfaces

Auteurs : Scott F. Dowell [Géorgie (pays), Thaïlande] ; James M. Simmerman [Géorgie (pays), Thaïlande] ; Dean D. Erdman [Thaïlande] ; Jiunn-Shyan Julian Wu [Thaïlande] ; Achara Chaovavanich [Géorgie (pays)] ; Massoud Javadi [Géorgie (pays), Thaïlande] ; Jyh-Yuan Yang [Thaïlande] ; Larry J. Anderson [Thaïlande] ; Suxiang Tong [Thaïlande] ; Mei Shang Ho [Taïwan]

Source :

RBID : PMC:7107915

Descripteurs français

English descriptors

Abstract

Abstract

Background. Health care workers continued to contract severe acute respiratory syndrome (SARS), even after barrier precautions were widely implemented.

Methods. We explored the possible contribution of contaminated hospital surfaces to SARS transmission by swabbing surfaces in 2 hospitals and testing the swab samples by reverse-transcriptase polymerase chain reaction (RT-PCR) and viral culture.

Results. Twenty-six of 94 swab samples tested positive for viral RNA. Swab samples of respiratory secretions from each of the 4 patients examined tested positive by RT-PCR, as were 12 of 43 swabs from patient rooms and 10 of 47 swabs from other parts of the hospital, including the computer mouses at 2 nursing stations and the handrail of the public elevator. Specimens from areas with patients with SARS in the most infectious phase of illness (days 5–15 after onset) were more likely to be RNA positive than were swab specimens from elsewhere (24 of 63 samples vs. 2 of 31 samples; P = .001). All cultures showed no growth.

Conclusions. Although the viruses identified may have been noninfectious, health care workers should be aware that SARS coronavirus can contaminate environmental surfaces in the hospital, and fomites should be considered to be a possible mode of transmission of SARS.


Url:
DOI: 10.1086/422652
PubMed: 15356778
PubMed Central: 7107915


Affiliations:


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PMC:7107915

Le document en format XML

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,
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<name sortKey="Chaovavanich, Achara" sort="Chaovavanich, Achara" uniqKey="Chaovavanich A" first="Achara" last="Chaovavanich">Achara Chaovavanich</name>
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,
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<name sortKey="Javadi, Massoud" sort="Javadi, Massoud" uniqKey="Javadi M" first="Massoud" last="Javadi">Massoud Javadi</name>
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<institution>International Emerging Infections Program, Centers for Disease Control and Prevention</institution>
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<name sortKey="Yang, Jyh Yuan" sort="Yang, Jyh Yuan" uniqKey="Yang J" first="Jyh-Yuan" last="Yang">Jyh-Yuan Yang</name>
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<institution>Department of Disease Control, Ministry of Public Health</institution>
,
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<name sortKey="Tong, Suxiang" sort="Tong, Suxiang" uniqKey="Tong S" first="Suxiang" last="Tong">Suxiang Tong</name>
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,
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</nlm:aff>
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<name sortKey="Ho, Mei Shang" sort="Ho, Mei Shang" uniqKey="Ho M" first="Mei Shang" last="Ho">Mei Shang Ho</name>
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,
<addr-line>Taipei, Taiwan</addr-line>
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<country xml:lang="fr">Taïwan</country>
<wicri:regionArea>Taipei</wicri:regionArea>
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,
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<term>Cross Infection (virology)</term>
<term>Disease Transmission, Infectious</term>
<term>Environmental Microbiology</term>
<term>Equipment and Supplies, Hospital (virology)</term>
<term>Genome, Viral</term>
<term>Hospitals (trends)</term>
<term>Humans</term>
<term>RNA, Viral (isolation & purification)</term>
<term>SARS Virus (genetics)</term>
<term>SARS Virus (isolation & purification)</term>
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<term>ARN viral (isolement et purification)</term>
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<term>Hôpitaux (tendances)</term>
<term>Infection croisée (virologie)</term>
<term>Maladies transmissibles émergentes (virologie)</term>
<term>Microbiologie de l'environnement</term>
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<term>Syndrome respiratoire aigu sévère (transmission)</term>
<term>Transmission de maladie infectieuse</term>
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<term>Virus du SRAS (isolement et purification)</term>
<term>Équipement et fournitures hospitaliers (virologie)</term>
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<term>RNA, Viral</term>
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<term>SARS Virus</term>
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<term>Virus du SRAS</term>
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<keywords scheme="MESH" qualifier="isolation & purification" xml:lang="en">
<term>SARS Virus</term>
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<term>Virus du SRAS</term>
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<term>Communicable Disease Control</term>
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<term>Prélèvement biologique</term>
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<term>Specimen Handling</term>
</keywords>
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<term>Hôpitaux</term>
</keywords>
<keywords scheme="MESH" qualifier="transmission" xml:lang="en">
<term>Severe Acute Respiratory Syndrome</term>
</keywords>
<keywords scheme="MESH" qualifier="trends" xml:lang="en">
<term>Hospitals</term>
</keywords>
<keywords scheme="MESH" qualifier="virologie" xml:lang="fr">
<term>Infection croisée</term>
<term>Maladies transmissibles émergentes</term>
<term>Équipement et fournitures hospitaliers</term>
</keywords>
<keywords scheme="MESH" qualifier="virology" xml:lang="en">
<term>Communicable Diseases, Emerging</term>
<term>Cross Infection</term>
<term>Equipment and Supplies, Hospital</term>
</keywords>
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<term>Disease Transmission, Infectious</term>
<term>Environmental Microbiology</term>
<term>Genome, Viral</term>
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<term>Génome viral</term>
<term>Humains</term>
<term>Microbiologie de l'environnement</term>
<term>Syndrome respiratoire aigu sévère</term>
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<div type="abstract" xml:lang="en">
<title>Abstract</title>
<p>
<bold>
<italic>Background.</italic>
</bold>
Health care workers continued to contract severe acute respiratory syndrome (SARS), even after barrier precautions were widely implemented.</p>
<p>
<bold>
<italic>Methods.</italic>
</bold>
We explored the possible contribution of contaminated hospital surfaces to SARS transmission by swabbing surfaces in 2 hospitals and testing the swab samples by reverse-transcriptase polymerase chain reaction (RT-PCR) and viral culture.</p>
<p>
<bold>
<italic>Results.</italic>
</bold>
Twenty-six of 94 swab samples tested positive for viral RNA. Swab samples of respiratory secretions from each of the 4 patients examined tested positive by RT-PCR, as were 12 of 43 swabs from patient rooms and 10 of 47 swabs from other parts of the hospital, including the computer mouses at 2 nursing stations and the handrail of the public elevator. Specimens from areas with patients with SARS in the most infectious phase of illness (days 5–15 after onset) were more likely to be RNA positive than were swab specimens from elsewhere (24 of 63 samples vs. 2 of 31 samples;
<italic>P</italic>
= .001). All cultures showed no growth.</p>
<p>
<bold>
<italic>Conclusions.</italic>
</bold>
Although the viruses identified may have been noninfectious, health care workers should be aware that SARS coronavirus can contaminate environmental surfaces in the hospital, and fomites should be considered to be a possible mode of transmission of SARS.</p>
</div>
</front>
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<name sortKey="Olsen, S" uniqKey="Olsen S">S Olsen</name>
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<name sortKey="Chang, H" uniqKey="Chang H">H Chang</name>
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<author>
<name sortKey="Cheung, T" uniqKey="Cheung T">T Cheung</name>
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<name sortKey="Simmerman, James M" sort="Simmerman, James M" uniqKey="Simmerman J" first="James M." last="Simmerman">James M. Simmerman</name>
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<name sortKey="Wu, Jiunn Shyan Julian" sort="Wu, Jiunn Shyan Julian" uniqKey="Wu J" first="Jiunn-Shyan Julian" last="Wu">Jiunn-Shyan Julian Wu</name>
<name sortKey="Yang, Jyh Yuan" sort="Yang, Jyh Yuan" uniqKey="Yang J" first="Jyh-Yuan" last="Yang">Jyh-Yuan Yang</name>
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</record>

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