Mouse hepatitis virus spike and nucleocapsid proteins expressed by adenovirus vectors protect mice against a lethal infection.
Identifieur interne : 006B02 ( Main/Merge ); précédent : 006B01; suivant : 006B03Mouse hepatitis virus spike and nucleocapsid proteins expressed by adenovirus vectors protect mice against a lethal infection.
Auteurs : J G Wesseling [Pays-Bas] ; G J Godeke ; V E Schijns ; L. Prevec ; F L Graham ; M C Horzinek ; P J RottierSource :
- The Journal of general virology [ 0022-1317 ] ; 1993.
Descripteurs français
- KwdFr :
- Adénovirus humains, Animaux, Anticorps antiviraux (sang), Capside (génétique), Capside (immunologie), Cellules HeLa, Clonage moléculaire, Femelle, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires, Gènes viraux (génétique), Humains, Hépatite virale animale (immunologie), Infections à coronavirus (immunologie), Infections à coronavirus (médecine vétérinaire), Protéines de l'enveloppe virale (génétique), Protéines de l'enveloppe virale (immunologie), Protéines du core viral (génétique), Protéines du core viral (immunologie), Recombinaison génétique, Souris, Souris de lignée BALB C, Vecteurs génétiques, Virus de l'hépatite murine (génétique), Virus de l'hépatite murine (immunologie).
- MESH :
- génétique : Capside, Gènes viraux, Protéines de l'enveloppe virale, Protéines du core viral, Virus de l'hépatite murine.
- immunologie : Capside, Hépatite virale animale, Infections à coronavirus, Protéines de l'enveloppe virale, Protéines du core viral, Virus de l'hépatite murine.
- médecine vétérinaire : Infections à coronavirus.
- sang : Anticorps antiviraux.
- Adénovirus humains, Animaux, Cellules HeLa, Clonage moléculaire, Femelle, Glycoprotéine de spicule des coronavirus, Glycoprotéines membranaires, Humains, Recombinaison génétique, Souris, Souris de lignée BALB C, Vecteurs génétiques.
English descriptors
- KwdEn :
- Adenoviruses, Human, Animals, Antibodies, Viral (blood), Capsid (genetics), Capsid (immunology), Cloning, Molecular, Coronavirus Infections (immunology), Coronavirus Infections (veterinary), Female, Genes, Viral (genetics), Genetic Vectors, HeLa Cells, Hepatitis, Viral, Animal (immunology), Humans, Membrane Glycoproteins, Mice, Mice, Inbred BALB C, Murine hepatitis virus (genetics), Murine hepatitis virus (immunology), Recombination, Genetic, Spike Glycoprotein, Coronavirus, Viral Core Proteins (genetics), Viral Core Proteins (immunology), Viral Envelope Proteins (genetics), Viral Envelope Proteins (immunology).
- MESH :
- chemical , blood : Antibodies, Viral.
- genetics : Capsid, Genes, Viral, Murine hepatitis virus, Viral Core Proteins, Viral Envelope Proteins.
- immunology : Capsid, Coronavirus Infections, Hepatitis, Viral, Animal, Murine hepatitis virus, Viral Core Proteins, Viral Envelope Proteins.
- veterinary : Coronavirus Infections.
- Adenoviruses, Human, Animals, Cloning, Molecular, Female, Genetic Vectors, HeLa Cells, Humans, Membrane Glycoproteins, Mice, Mice, Inbred BALB C, Recombination, Genetic, Spike Glycoprotein, Coronavirus.
Abstract
Infection with the mouse hepatitis coronavirus (MHV) provides an excellent model for the study of viral diseases of the central nervous system and the gastrointestinal tract. With the ultimate aim of studying mucosal immunity to MHV we have cloned the genes encoding the structural proteins of MHV strain A59 (MHV-A59) into the E3 region of a human adenovirus type 5 vector. Infection of HeLa cells with the resulting recombinant adenoviruses AdMHVS, AdMHVN and AdMHVM revealed the correct expression of the spike (S), nucleocapsid (N) and membrane (M) proteins, respectively. Intraperitoneal inoculation of BALB/c mice with the recombinant viruses elicited serum antibodies which specifically recognized the respective MHV proteins in an immunoprecipitation assay. Only antibodies to the S protein neutralized MHV-A59 in vitro but titres were low. When analysed by ELISA or by immunofluorescence only the antibody response to the N protein was significant; weak responses or no detectable response at all were found for S and M, respectively. Upon intracerebral challenge with a lethal dose of MHV-A59 we found that a significant fraction of animals vaccinated with adenovirus vectors expressing either the S protein or N protein were protected. This protective effect was significantly stronger when the animals were given a booster immunization with the same vector prior to challenge. No protection was induced by AdMHVM. Interestingly, enhanced protection resulted when AdMHVS and AdMHVN were applied in combination as compared to survival after single immunizations. The results indicate that both the N and S proteins generate a protective immune response and suggest that this response is enhanced by combined expression of the two proteins.
DOI: 10.1099/0022-1317-74-10-2061
PubMed: 8409930
Links toward previous steps (curation, corpus...)
- to stream PubMed, to step Corpus: 003536
- to stream PubMed, to step Curation: 003536
- to stream PubMed, to step Checkpoint: 003427
- to stream Ncbi, to step Merge: 003C33
- to stream Ncbi, to step Curation: 003C33
- to stream Ncbi, to step Checkpoint: 003C33
Links to Exploration step
pubmed:8409930Le document en format XML
<record><TEI><teiHeader><fileDesc><titleStmt><title xml:lang="en">Mouse hepatitis virus spike and nucleocapsid proteins expressed by adenovirus vectors protect mice against a lethal infection.</title><author><name sortKey="Wesseling, J G" sort="Wesseling, J G" uniqKey="Wesseling J" first="J G" last="Wesseling">J G Wesseling</name><affiliation wicri:level="4"><nlm:affiliation>Institute of Virology, Veterinary Faculty, Utrecht University, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Institute of Virology, Veterinary Faculty, Utrecht University</wicri:regionArea><placeName><settlement type="city">Utrecht</settlement><region nuts="2">Utrecht (province)</region></placeName><orgName type="university">Université d'Utrecht</orgName></affiliation></author><author><name sortKey="Godeke, G J" sort="Godeke, G J" uniqKey="Godeke G" first="G J" last="Godeke">G J Godeke</name></author><author><name sortKey="Schijns, V E" sort="Schijns, V E" uniqKey="Schijns V" first="V E" last="Schijns">V E Schijns</name></author><author><name sortKey="Prevec, L" sort="Prevec, L" uniqKey="Prevec L" first="L" last="Prevec">L. Prevec</name></author><author><name sortKey="Graham, F L" sort="Graham, F L" uniqKey="Graham F" first="F L" last="Graham">F L Graham</name></author><author><name sortKey="Horzinek, M C" sort="Horzinek, M C" uniqKey="Horzinek M" first="M C" last="Horzinek">M C Horzinek</name></author><author><name sortKey="Rottier, P J" sort="Rottier, P J" uniqKey="Rottier P" first="P J" last="Rottier">P J Rottier</name></author></titleStmt><publicationStmt><idno type="wicri:source">PubMed</idno><date when="1993">1993</date><idno type="RBID">pubmed:8409930</idno><idno type="pmid">8409930</idno><idno type="doi">10.1099/0022-1317-74-10-2061</idno><idno type="wicri:Area/PubMed/Corpus">003536</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Corpus" wicri:corpus="PubMed">003536</idno><idno type="wicri:Area/PubMed/Curation">003536</idno><idno type="wicri:explorRef" wicri:stream="PubMed" wicri:step="Curation">003536</idno><idno type="wicri:Area/PubMed/Checkpoint">003427</idno><idno type="wicri:explorRef" wicri:stream="Checkpoint" wicri:step="PubMed">003427</idno><idno type="wicri:Area/Ncbi/Merge">003C33</idno><idno type="wicri:Area/Ncbi/Curation">003C33</idno><idno type="wicri:Area/Ncbi/Checkpoint">003C33</idno><idno type="wicri:doubleKey">0022-1317:1993:Wesseling J:mouse:hepatitis:virus</idno><idno type="wicri:Area/Main/Merge">006B02</idno></publicationStmt><sourceDesc><biblStruct><analytic><title xml:lang="en">Mouse hepatitis virus spike and nucleocapsid proteins expressed by adenovirus vectors protect mice against a lethal infection.</title><author><name sortKey="Wesseling, J G" sort="Wesseling, J G" uniqKey="Wesseling J" first="J G" last="Wesseling">J G Wesseling</name><affiliation wicri:level="4"><nlm:affiliation>Institute of Virology, Veterinary Faculty, Utrecht University, The Netherlands.</nlm:affiliation><country xml:lang="fr">Pays-Bas</country><wicri:regionArea>Institute of Virology, Veterinary Faculty, Utrecht University</wicri:regionArea><placeName><settlement type="city">Utrecht</settlement><region nuts="2">Utrecht (province)</region></placeName><orgName type="university">Université d'Utrecht</orgName></affiliation></author><author><name sortKey="Godeke, G J" sort="Godeke, G J" uniqKey="Godeke G" first="G J" last="Godeke">G J Godeke</name></author><author><name sortKey="Schijns, V E" sort="Schijns, V E" uniqKey="Schijns V" first="V E" last="Schijns">V E Schijns</name></author><author><name sortKey="Prevec, L" sort="Prevec, L" uniqKey="Prevec L" first="L" last="Prevec">L. Prevec</name></author><author><name sortKey="Graham, F L" sort="Graham, F L" uniqKey="Graham F" first="F L" last="Graham">F L Graham</name></author><author><name sortKey="Horzinek, M C" sort="Horzinek, M C" uniqKey="Horzinek M" first="M C" last="Horzinek">M C Horzinek</name></author><author><name sortKey="Rottier, P J" sort="Rottier, P J" uniqKey="Rottier P" first="P J" last="Rottier">P J Rottier</name></author></analytic><series><title level="j">The Journal of general virology</title><idno type="ISSN">0022-1317</idno><imprint><date when="1993" type="published">1993</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Adenoviruses, Human</term><term>Animals</term><term>Antibodies, Viral (blood)</term><term>Capsid (genetics)</term><term>Capsid (immunology)</term><term>Cloning, Molecular</term><term>Coronavirus Infections (immunology)</term><term>Coronavirus Infections (veterinary)</term><term>Female</term><term>Genes, Viral (genetics)</term><term>Genetic Vectors</term><term>HeLa Cells</term><term>Hepatitis, Viral, Animal (immunology)</term><term>Humans</term><term>Membrane Glycoproteins</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Murine hepatitis virus (genetics)</term><term>Murine hepatitis virus (immunology)</term><term>Recombination, Genetic</term><term>Spike Glycoprotein, Coronavirus</term><term>Viral Core Proteins (genetics)</term><term>Viral Core Proteins (immunology)</term><term>Viral Envelope Proteins (genetics)</term><term>Viral Envelope Proteins (immunology)</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Adénovirus humains</term><term>Animaux</term><term>Anticorps antiviraux (sang)</term><term>Capside (génétique)</term><term>Capside (immunologie)</term><term>Cellules HeLa</term><term>Clonage moléculaire</term><term>Femelle</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires</term><term>Gènes viraux (génétique)</term><term>Humains</term><term>Hépatite virale animale (immunologie)</term><term>Infections à coronavirus (immunologie)</term><term>Infections à coronavirus (médecine vétérinaire)</term><term>Protéines de l'enveloppe virale (génétique)</term><term>Protéines de l'enveloppe virale (immunologie)</term><term>Protéines du core viral (génétique)</term><term>Protéines du core viral (immunologie)</term><term>Recombinaison génétique</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Vecteurs génétiques</term><term>Virus de l'hépatite murine (génétique)</term><term>Virus de l'hépatite murine (immunologie)</term></keywords><keywords scheme="MESH" type="chemical" qualifier="blood" xml:lang="en"><term>Antibodies, Viral</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Capsid</term><term>Genes, Viral</term><term>Murine hepatitis virus</term><term>Viral Core Proteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Capside</term><term>Gènes viraux</term><term>Protéines de l'enveloppe virale</term><term>Protéines du core viral</term><term>Virus de l'hépatite murine</term></keywords><keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Capside</term><term>Hépatite virale animale</term><term>Infections à coronavirus</term><term>Protéines de l'enveloppe virale</term><term>Protéines du core viral</term><term>Virus de l'hépatite murine</term></keywords><keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>Capsid</term><term>Coronavirus Infections</term><term>Hepatitis, Viral, Animal</term><term>Murine hepatitis virus</term><term>Viral Core Proteins</term><term>Viral Envelope Proteins</term></keywords><keywords scheme="MESH" qualifier="médecine vétérinaire" xml:lang="fr"><term>Infections à coronavirus</term></keywords><keywords scheme="MESH" qualifier="sang" xml:lang="fr"><term>Anticorps antiviraux</term></keywords><keywords scheme="MESH" qualifier="veterinary" xml:lang="en"><term>Coronavirus Infections</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Adenoviruses, Human</term><term>Animals</term><term>Cloning, Molecular</term><term>Female</term><term>Genetic Vectors</term><term>HeLa Cells</term><term>Humans</term><term>Membrane Glycoproteins</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Recombination, Genetic</term><term>Spike Glycoprotein, Coronavirus</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Adénovirus humains</term><term>Animaux</term><term>Cellules HeLa</term><term>Clonage moléculaire</term><term>Femelle</term><term>Glycoprotéine de spicule des coronavirus</term><term>Glycoprotéines membranaires</term><term>Humains</term><term>Recombinaison génétique</term><term>Souris</term><term>Souris de lignée BALB C</term><term>Vecteurs génétiques</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">Infection with the mouse hepatitis coronavirus (MHV) provides an excellent model for the study of viral diseases of the central nervous system and the gastrointestinal tract. With the ultimate aim of studying mucosal immunity to MHV we have cloned the genes encoding the structural proteins of MHV strain A59 (MHV-A59) into the E3 region of a human adenovirus type 5 vector. Infection of HeLa cells with the resulting recombinant adenoviruses AdMHVS, AdMHVN and AdMHVM revealed the correct expression of the spike (S), nucleocapsid (N) and membrane (M) proteins, respectively. Intraperitoneal inoculation of BALB/c mice with the recombinant viruses elicited serum antibodies which specifically recognized the respective MHV proteins in an immunoprecipitation assay. Only antibodies to the S protein neutralized MHV-A59 in vitro but titres were low. When analysed by ELISA or by immunofluorescence only the antibody response to the N protein was significant; weak responses or no detectable response at all were found for S and M, respectively. Upon intracerebral challenge with a lethal dose of MHV-A59 we found that a significant fraction of animals vaccinated with adenovirus vectors expressing either the S protein or N protein were protected. This protective effect was significantly stronger when the animals were given a booster immunization with the same vector prior to challenge. No protection was induced by AdMHVM. Interestingly, enhanced protection resulted when AdMHVS and AdMHVN were applied in combination as compared to survival after single immunizations. The results indicate that both the N and S proteins generate a protective immune response and suggest that this response is enhanced by combined expression of the two proteins.</div></front></TEI></record>Pour manipuler ce document sous Unix (Dilib)
EXPLOR_STEP=$WICRI_ROOT/Sante/explor/SrasV1/Data/Main/Merge
HfdSelect -h $EXPLOR_STEP/biblio.hfd -nk 006B02 | SxmlIndent | more
Ou
HfdSelect -h $EXPLOR_AREA/Data/Main/Merge/biblio.hfd -nk 006B02 | SxmlIndent | more
Pour mettre un lien sur cette page dans le réseau Wicri
{{Explor lien
|wiki= Sante
|area= SrasV1
|flux= Main
|étape= Merge
|type= RBID
|clé= pubmed:8409930
|texte= Mouse hepatitis virus spike and nucleocapsid proteins expressed by adenovirus vectors protect mice against a lethal infection.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Merge/RBID.i -Sk "pubmed:8409930" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Merge/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |