SrasV1, Main, Merge, bibRecord, 006222

The Structural Characterization and Antigenicity of the S Protein of SARS-CoV

Identifieur interne : 006222 ( Main/Merge ); précédent : 006221; suivant : 006223

The Structural Characterization and Antigenicity of the S Protein of SARS-CoV

Source :

Genomics, Proteomics & Bioinformatics [ 1672-0229 ] ; 2003.

RBID : PMC:5172354

Descripteurs français

English descriptors

KwdEn :
- Amino Acid Sequence, Antigens, Viral (immunology), Base Composition, Computational Biology, Enzyme-Linked Immunosorbent Assay, Membrane Glycoproteins (genetics), Molecular Sequence Data, Mutation (genetics), Phylogeny, Protein Structure, Tertiary, SARS Virus (genetics), SARS Virus (immunology), Sequence Analysis, DNA, Sequence Homology, Spike Glycoprotein, Coronavirus, Viral Envelope Proteins (genetics), Viral Envelope Proteins (metabolism).
MESH :
- chemical , genetics : Membrane Glycoproteins, Viral Envelope Proteins.
- chemical , immunology : Antigens, Viral.
- genetics : Mutation, SARS Virus.
- immunology : SARS Virus.
- chemical , metabolism : Viral Envelope Proteins.
- Amino Acid Sequence, Base Composition, Computational Biology, Enzyme-Linked Immunosorbent Assay, Molecular Sequence Data, Phylogeny, Protein Structure, Tertiary, Sequence Analysis, DNA, Sequence Homology, Spike Glycoprotein, Coronavirus.

Abstract

The corona-like spikes or peplomers on the surface of the virion under electronic microscope are the most striking features of coronaviruses. The S (spike) protein is the largest structural protein, with 1,255 amino acids, in the viral genome. Its structure can be divided into three regions: a long N-terminal region in the exterior, a characteristic transmembrane (TM) region, and a short C-terminus in the interior of a virion. We detected fifteen substitutions of nucleotides by comparisons with the seventeen published SARS-CoV genome sequences, eight (53.3%) of which are non-synonymous mutations leading to amino acid alternations with predicted physiochemical changes. The possible antigenic determinants of the S protein are predicted, and the result is confirmed by ELISA (enzyme-linked immunosorbent assay) with synthesized peptides. Another profound finding is that three disulfide bonds are defined at the C-terminus with the N-terminus of the E (envelope) protein, based on the typical sequence and positions, thus establishing the structural connection with these two important structural proteins, if confirmed. Phylogenetic analysis reveals several conserved regions that might be potent drug targets.

Url:

http://www.ncbi.nlm.nih.gov/pmc/articles/PMC5172354

DOI: 10.1016/S1672-0229(03)01015-5
PubMed: 15626341
PubMed Central: 5172354

Links to Exploration step

PMC:5172354

Le document en format XML

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<author><name sortKey="Wang, Jing" sort="Wang, Jing" uniqKey="Wang J" first="Jing" last="Wang">Jing Wang</name>
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<orgName type="university">Université de Zhejiang</orgName>
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</affiliation>
</author>
<author><name sortKey="Tong, Wei" sort="Tong, Wei" uniqKey="Tong W" first="Wei" last="Tong">Wei Tong</name>
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<author><name sortKey="Wei, Wei" sort="Wei, Wei" uniqKey="Wei W" first="Wei" last="Wei">Wei Wei</name>
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<placeName><settlement type="city">Hangzhou</settlement>
<region type="province">Zhejiang</region>
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<orgName type="university">Université de Zhejiang</orgName>
<placeName><settlement type="city">Hangzhou</settlement>
<region type="province">Zhejiang</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Zhang, Qingrun" sort="Zhang, Qingrun" uniqKey="Zhang Q" first="Qingrun" last="Zhang">Qingrun Zhang</name>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
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<author><name sortKey="Li, Shengbin" sort="Li, Shengbin" uniqKey="Li S" first="Shengbin" last="Li">Shengbin Li</name>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="1"><nlm:aff id="aff0010">Medical College, Xi’an Jiaotong University, Xi’an 710049, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Medical College, Xi’an Jiaotong University, Xi’an 710049</wicri:regionArea>
<wicri:noRegion>Xi’an 710049</wicri:noRegion>
</affiliation>
</author>
<author><name sortKey="Li, Wei" sort="Li, Wei" uniqKey="Li W" first="Wei" last="Li">Wei Li</name>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
<placeName><settlement type="city">Pékin</settlement>
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<author><name sortKey="Li, Hongyan" sort="Li, Hongyan" uniqKey="Li H" first="Hongyan" last="Li">Hongyan Li</name>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Li, Yudong" sort="Li, Yudong" uniqKey="Li Y" first="Yudong" last="Li">Yudong Li</name>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Dong, Wei" sort="Dong, Wei" uniqKey="Dong W" first="Wei" last="Dong">Wei Dong</name>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Wang, Jian" sort="Wang, Jian" uniqKey="Wang J" first="Jian" last="Wang">Jian Wang</name>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="4"><nlm:aff id="aff0020">James D. Watson Institute of Genome Sciences, Zhijiang Campus, Zhejiang University, Hangzhou 310008, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>James D. Watson Institute of Genome Sciences, Zhijiang Campus, Zhejiang University, Hangzhou 310008</wicri:regionArea>
<placeName><settlement type="city">Hangzhou</settlement>
<region type="province">Zhejiang</region>
</placeName>
<orgName type="university">Université de Zhejiang</orgName>
<placeName><settlement type="city">Hangzhou</settlement>
<region type="province">Zhejiang</region>
</placeName>
</affiliation>
</author>
<author><name sortKey="Bi, Shengli" sort="Bi, Shengli" uniqKey="Bi S" first="Shengli" last="Bi">Shengli Bi</name>
<affiliation wicri:level="1"><nlm:aff id="aff0025">Center of Disease Control and Prevention, Beijing 100050, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Center of Disease Control and Prevention, Beijing 100050</wicri:regionArea>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
</author>
<author><name sortKey="Yang, Huanming" sort="Yang, Huanming" uniqKey="Yang H" first="Huanming" last="Yang">Huanming Yang</name>
<affiliation wicri:level="1"><nlm:aff id="aff0005">Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>Beijing Genomics Institute, Chinese Academy of Sciences, Beijing 101300</wicri:regionArea>
<placeName><settlement type="city">Pékin</settlement>
</placeName>
</affiliation>
<affiliation wicri:level="4"><nlm:aff id="aff0020">James D. Watson Institute of Genome Sciences, Zhijiang Campus, Zhejiang University, Hangzhou 310008, China</nlm:aff>
<country xml:lang="fr">République populaire de Chine</country>
<wicri:regionArea>James D. Watson Institute of Genome Sciences, Zhijiang Campus, Zhejiang University, Hangzhou 310008</wicri:regionArea>
<placeName><settlement type="city">Hangzhou</settlement>
<region type="province">Zhejiang</region>
</placeName>
<orgName type="university">Université de Zhejiang</orgName>
<placeName><settlement type="city">Hangzhou</settlement>
<region type="province">Zhejiang</region>
</placeName>
</affiliation>
</author>
</analytic>
<series><title level="j">Genomics, Proteomics & Bioinformatics</title>
<idno type="ISSN">1672-0229</idno>
<idno type="eISSN">2210-3244</idno>
<imprint><date when="2003">2003</date>
</imprint>
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<profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Antigens, Viral (immunology)</term>
<term>Base Composition</term>
<term>Computational Biology</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Membrane Glycoproteins (genetics)</term>
<term>Molecular Sequence Data</term>
<term>Mutation (genetics)</term>
<term>Phylogeny</term>
<term>Protein Structure, Tertiary</term>
<term>SARS Virus (genetics)</term>
<term>SARS Virus (immunology)</term>
<term>Sequence Analysis, DNA</term>
<term>Sequence Homology</term>
<term>Spike Glycoprotein, Coronavirus</term>
<term>Viral Envelope Proteins (genetics)</term>
<term>Viral Envelope Proteins (metabolism)</term>
</keywords>
<keywords scheme="KwdFr" xml:lang="fr"><term>Analyse de séquence d'ADN</term>
<term>Antigènes viraux (immunologie)</term>
<term>Biologie informatique</term>
<term>Composition en bases nucléiques</term>
<term>Données de séquences moléculaires</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Glycoprotéines membranaires (génétique)</term>
<term>Mutation (génétique)</term>
<term>Phylogénie</term>
<term>Protéines de l'enveloppe virale (génétique)</term>
<term>Protéines de l'enveloppe virale (métabolisme)</term>
<term>Similitude de séquences</term>
<term>Structure tertiaire des protéines</term>
<term>Séquence d'acides aminés</term>
<term>Test ELISA</term>
<term>Virus du SRAS (génétique)</term>
<term>Virus du SRAS (immunologie)</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="genetics" xml:lang="en"><term>Membrane Glycoproteins</term>
<term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="immunology" xml:lang="en"><term>Antigens, Viral</term>
</keywords>
<keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>Mutation</term>
<term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Glycoprotéines membranaires</term>
<term>Mutation</term>
<term>Protéines de l'enveloppe virale</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="immunologie" xml:lang="fr"><term>Antigènes viraux</term>
<term>Virus du SRAS</term>
</keywords>
<keywords scheme="MESH" qualifier="immunology" xml:lang="en"><term>SARS Virus</term>
</keywords>
<keywords scheme="MESH" type="chemical" qualifier="metabolism" xml:lang="en"><term>Viral Envelope Proteins</term>
</keywords>
<keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Protéines de l'enveloppe virale</term>
</keywords>
<keywords scheme="MESH" xml:lang="en"><term>Amino Acid Sequence</term>
<term>Base Composition</term>
<term>Computational Biology</term>
<term>Enzyme-Linked Immunosorbent Assay</term>
<term>Molecular Sequence Data</term>
<term>Phylogeny</term>
<term>Protein Structure, Tertiary</term>
<term>Sequence Analysis, DNA</term>
<term>Sequence Homology</term>
<term>Spike Glycoprotein, Coronavirus</term>
</keywords>
<keywords scheme="MESH" xml:lang="fr"><term>Analyse de séquence d'ADN</term>
<term>Biologie informatique</term>
<term>Composition en bases nucléiques</term>
<term>Données de séquences moléculaires</term>
<term>Glycoprotéine de spicule des coronavirus</term>
<term>Phylogénie</term>
<term>Similitude de séquences</term>
<term>Structure tertiaire des protéines</term>
<term>Séquence d'acides aminés</term>
<term>Test ELISA</term>
</keywords>
</textClass>
</profileDesc>
</teiHeader>
<front><div type="abstract" xml:lang="en"><p>The corona-like spikes or peplomers on the surface of the virion under electronic microscope are the most striking features of coronaviruses. The S (spike) protein is the largest structural protein, with 1,255 amino acids, in the viral genome. Its structure can be divided into three regions: a long N-terminal region in the exterior, a characteristic transmembrane (TM) region, and a short C-terminus in the interior of a virion. We detected fifteen substitutions of nucleotides by comparisons with the seventeen published SARS-CoV genome sequences, eight (53.3%) of which are non-synonymous mutations leading to amino acid alternations with predicted physiochemical changes. The possible antigenic determinants of the S protein are predicted, and the result is confirmed by ELISA (enzyme-linked immunosorbent assay) with synthesized peptides. Another profound finding is that three disulfide bonds are defined at the C-terminus with the N-terminus of the E (envelope) protein, based on the typical sequence and positions, thus establishing the structural connection with these two important structural proteins, if confirmed. Phylogenetic analysis reveals several conserved regions that might be potent drug targets.</p>
</div>
</front>
<back><div1 type="bibliography"><listBibl><biblStruct><analytic><author><name sortKey="Jackwood, M W" uniqKey="Jackwood M">M.W. Jackwood</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Frana, M F" uniqKey="Frana M">M.F. Frana</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Cavanagh, D" uniqKey="Cavanagh D">D. Cavanagh</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Abraham, S" uniqKey="Abraham S">S. Abraham</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Garoff, H" uniqKey="Garoff H">H. Garoff</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Gallagher, T M" uniqKey="Gallagher T">T.M. Gallagher</name>
</author>
<author><name sortKey="Buchmeier, M J" uniqKey="Buchmeier M">M.J. Buchmeier</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Bos, E C" uniqKey="Bos E">E.C. Bos</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ballesteros, M L" uniqKey="Ballesteros M">M.L. Ballesteros</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Marra, M A" uniqKey="Marra M">M.A. Marra</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ruan, Y J" uniqKey="Ruan Y">Y.J. Ruan</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Qin, E D" uniqKey="Qin E">E.D. Qin</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rota, P A" uniqKey="Rota P">P.A. Rota</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Ignjatovic, J" uniqKey="Ignjatovic J">J. Ignjatovic</name>
</author>
<author><name sortKey="Galli, L" uniqKey="Galli L">L. Galli</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Leparc Goffart, I" uniqKey="Leparc Goffart I">I. Leparc-Goffart</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Kusters, J G" uniqKey="Kusters J">J.G. Kusters</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="De Groot, R J" uniqKey="De Groot R">R.J. de Groot</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Rapaport, D" uniqKey="Rapaport D">D. Rapaport</name>
</author>
</analytic>
</biblStruct>
<biblStruct><analytic><author><name sortKey="Opstelten, D J" uniqKey="Opstelten D">D.J. Opstelten</name>
</author>
</analytic>
</biblStruct>
</listBibl>
</div1>
</back>
</TEI>
</record>

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The Structural Characterization and Antigenicity of the S Protein of SARS-CoV

The Structural Characterization and Antigenicity of the S Protein of SARS-CoV

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